Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,387,908	C→T	P397S (CCG→TCG)	tyrR →	DNA‑binding transcriptional dual regulator TyrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,387,908	0	C	T	98.8%	282.8 / NA	83	P397S (CCG→TCG)	tyrR	DNA‑binding transcriptional dual regulator TyrR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (32/49);  minor base G (0/1);  total (32/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCG‑AACTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCG  >  NC_000913/1387666‑1388152                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     atgatgGCACTTTCCGTCGGGTTGGCGAAGACAATGAGGTGCCTGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATATGGTCG‑AACTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTg                                                                                                                                                                                                                                              >  1:512847/1‑251 (MQ=255)      ggCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGCAGCATCTGGTCG‑AACTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGc                                                                                                                                                                                                                                         >  1:269907/1‑251 (MQ=255)           tttCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCG‑AACTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTgccc                                                                                                                                                                                                                                    >  1:411783/1‑248 (MQ=255)            ttCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGAGATTTGCGCTACGCAGAAGAATCTGGTCG‑AACTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGAccc                                                                                                                                                                                                                                   >  1:69534/1‑250 (MQ=255)              ccGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCG‑AACTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCCACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTg                                                                                                                                                                                                                                  >  1:547126/1‑250 (MQ=255)                gTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCG‑AACTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGact                                                                                                                                                                                                                               >  2:359857/1‑249 (MQ=255)                           gAAGACCATGGGATGCGTGTAGGTGTGCGGGTGATTTGCGCTACGCAGAAGAGTCTGGTCC‑AACTGGTGCCAGACG‑GCCTGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGCGCTGTTCGTCGCCCGCTTTGCCGACGAGCCGGGCGCGCCGCGTTCGAAACTGGCCGCTGCCCTGACTCCTGTACTTAc                                                                                                                                                                                                                    >  2:62320/1‑251 (MQ=255)                                ccATGAGGTGCATGTCGATGGGCGGGTGATTTGCGCTACGCAGAAGAACCTGGTCG‑AACTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGtt                                                                                                                                                                                                               <  2:145681/251‑1 (MQ=255)                                 cATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCG‑AACTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGtt                                                                                                                                                                                                               >  1:128413/1‑250 (MQ=255)                                       gTGCATGTCGTGGTGCGGGTGATTTGCGCTACGCAGAAGAATCGGGTCG‑AACTGGGGCAAAAAG‑GCATGTTCCGGGAAGATCTCTATTATCGTCGGAACGTGTGGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTg                                                                                                                                                                                                        <  1:576117/251‑1 (MQ=255)                                       gTGCATGTCGATGTGCGTGTGATTTGCGCTACGCAGAAGAATCTGGTCG‑AACTGGTGCAAAAAG‑GCATGTTCCGGGAAGATCTCTATTACCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTg                                                                                                                                                                                                        <  1:96572/251‑1 (MQ=255)                                           cggtgtaTGTGCGGGTGATGTCCGCGACGCGAAAGAATCTGGTCG‑AAATGGTTCACTAAGTGCGT‑TTCCGTTTGTATCTCGATTAGCGGCTGACCGGGTTGACGCTCATTCTACCGCCGTTACTGGACTGT‑CCGCATGACATCATTCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGTGCAGGGTGTGCAGCGTTCGAAACTTGCTTCTGACCTGAATACTTTACTTACGCGTTATGCGTGGCCg                                                                                                                                                                                                    <  2:531637/245‑1 (MQ=255)                                             gtcgtgttgCGGGTGATTTGCGTTACGCAGAAGAATCTGGTCG‑AACTGGTGCAAAAAG‑GCATGTTCAGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCgg                                                                                                                                                                                                   <  2:330943/243‑1 (MQ=255)                                             gTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCG‑AACTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCggg                                                                                                                                                                                                  >  1:114958/1‑251 (MQ=255)                                               cGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCG‑AACTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGa                                                                                                                                                                                                 >  2:169405/1‑250 (MQ=255)                                                  tgtgCGGGGGATTGGCGCTACGCAGAAGAATATGGTCG‑AAATGGTGCAAAAAG‑GCATGATCCGTGAAGATATCTATTCTCGGATGAAAGTGTTGACGCTCAATCTGCCGCCGCTACGTTACTGT‑CAGCAGGACATCATGCCGTTAACTGAGCTGTGCGTCGCACGCTTTGACGACGAGCAGGGCGTGCCGCGTTCGAACCTCGCCGCTGACCTGCATCCTGGACTTACGCGTTATGCGTGGCCGGGACAtg                                                                                                                                                                                             >  2:464462/1‑251 (MQ=255)                                                   gtgCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCG‑AACTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAAtg                                                                                                                                                                                             <  1:169405/250‑1 (MQ=255)                                                   gggCGGGTGATTTGCTCTACGCAGAAGAATCTGGTCG‑AACTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCGGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGGTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAAtgt                                                                                                                                                                                            <  2:257611/249‑1 (MQ=255)                                                     gCGGGGGATTTGCGCTACGCAGAAGAATC‑GGTGGGAACTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCGCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGc                                                                                                                                                                                          <  2:479619/251‑1 (MQ=255)                                                       gggTGATTTGCGCTACGCAGAAGAATCTGGTCG‑AACTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCgg                                                                                                                                                                                        <  1:358375/251‑1 (MQ=255)                                                         gtgattgtcgcgacgaggaagaaTCTGGTCG‑AACTGGTGAAAAAAG‑GCATTTCCCTTAAAGATCCTTATAATCGCCGGAACGTGTGGGCGCTCAAGCGGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACGGGCCGCTGACCGGAATACTTTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCa                                                                                                                                                                                      <  2:536868/234‑1 (MQ=255)                                                                        cAGAAGAATCTGGTCG‑ATCTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCGCGATTATCGTCGGAACGTGTTGTCGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGGTCGTCGCCCGCTTTGGCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGGACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGctat                                                                                                                                                                       <  1:81033/251‑1 (MQ=255)                                                                           aagaaTCTGGTCG‑AACTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCGGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGctatcta                                                                                                                                                                    <  1:44742/251‑1 (MQ=255)                                                                                  tGGTCG‑AACTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAAATGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCgcgcc                                                                                                                                                             >  2:516474/1‑250 (MQ=255)                                                                                   ggtgg‑AACTGGTTCAAAAAG‑GCATGTTCCGGGAAGATCTCTATTATCGTCGGAACGTGTTGACGCCCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCGGGGCGTGCCGCGTTCGAAACGGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCAc                                                                                                                                                            <  2:244840/247‑1 (MQ=255)                                                                                       g‑AACTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTAcg                                                                                                                                                                                                                   <  2:103499/192‑1 (MQ=255)                                                                                       g‑AACTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTAcg                                                                                                                                                                                                                   >  1:103499/1‑192 (MQ=255)                                                                                       g‑AACTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGac                                                                                                                                                        >  1:136606/1‑251 (MQ=255)                                                                                         aaCTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CAGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGGTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTGAAGAACGCTATCTATCGCGCACtcac                                                                                                                                                        >  1:365248/1‑247 (MQ=255)                                                                                          acgggggaaaaaaG‑GCAGGTTCGGTGAAGATCTCTATTAGCGTCTGAACGGGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGacac                                                                                                                                                      <  2:461273/243‑1 (MQ=255)                                                                                             ggggCAAAAAG‑TCATGTTCCGGGAAGATCCCTTTGTTCTTCTGAACGTGTTGGCGCTCAAGCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTAGCGCGCACTGACACAAc                                                                                                                                                   <  2:512833/248‑1 (MQ=255)                                                                                             ggTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAAc                                                                                                                                                   <  1:64117/251‑1 (MQ=255)                                                                                                  aaaaaG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑GCGCAGGAAATCATGCCGTTAACTGAGCTATTCGTCGCCCGCTTTGCCGACGAGCAGGACGTGCCGCGTTCGAAACTGGCCGCTGCCCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGCACGCTATCTATCGCGCACTGACACAACTGGAc                                                                                                                                              >  1:520963/1‑251 (MQ=255)                                                                                                      aG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGtt                                                                                                                                          <  1:411480/251‑1 (MQ=255)                                                                                                       aggaattttccgagaggttctcTATTGTCGTCTGAGCGTTTTGACGCGCATTCTGCCGCCGCTACGTGACGGT‑CCGCAGGACATCATGCCGTTAACCGAGCGGTTCGTCGCCCGCTTTGCCGACGAGCAGAGCGTGCCGCGTACGAAACTGGCCGCTGACCTGAATACGTTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCGCTGACAGAACTGGACGGtt                                                                                                                                          <  2:489512/233‑1 (MQ=255)                                                                                                         gCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTAt                                                                                                                                        <  1:2112/251‑1 (MQ=255)                                                                                                           aTGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATg                                                                                                                                       >  2:205573/1‑250 (MQ=255)                                                                                                               cccGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGTACGGTTATGAGCTg                                                                                                                                  <  1:165313/250‑1 (MQ=255)                                                                                                                  gTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGGCGGTTATGAGCTGCGt                                                                                                                               >  1:402358/1‑251 (MQ=255)                                                                                                                  gTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGt                                                                                                                               >  1:175725/1‑251 (MQ=255)                                                                                                                   gaaagagcgctATTATCGTCGGAACGGGTTGACGCTCATCCTGCCGCCGCTACGTGAC‑GTGCCGCAGGAGATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATGCTGTACTTACGCGTTATGCGTGGCCGGTAAATGTGCGGCAGGTAAAGAACGTTATCTTTTGCGCACTTACTCAACGGGATGTATATTAGCTGCGTc                                                                                                                              <  2:547126/242‑1 (MQ=255)                                                                                                                     aaGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGCATACTGTACTTACGCGTTATGCGTGGCCGGGCAATGTGCGGCAGTTAAAGCACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTcc                                                                                                                             >  1:585419/1‑250 (MQ=255)                                                                                                                     aaGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTcc                                                                                                                             >  1:424674/1‑250 (MQ=255)                                                                                                                            cttttatCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACGTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTGAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAgg                                                                                                                        <  1:40572/245‑1 (MQ=255)                                                                                                                            ctgataccGTCGGAACGTGTTGACGCTCAATCTGCCGCCTCTACGTTACTGT‑CCGCGGGCCATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAgg                                                                                                                        <  2:139691/241‑1 (MQ=255)                                                                                                                              attatCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGat                                                                                                                      <  1:570535/248‑1 (MQ=255)                                                                                                                                tatCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATAtttt                                                                                                                 >  1:472049/1‑251 (MQ=255)                                                                                                                                 atCGTCTGAACGTGTTGACGCTCAATCGGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACATGAGATTttg                                                                                                                <  1:461403/251‑1 (MQ=255)                                                                                                                                   cGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTttgtt                                                                                                              <  1:359857/251‑1 (MQ=255)                                                                                                                                        gAACGGGTTGACGCGCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGcc                                                                                                           <  2:303033/249‑1 (MQ=255)                                                                                                                                            gtgtTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGAtt                                                                                                      >  1:348320/1‑250 (MQ=255)                                                                                                                                              gtTGCCGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTAt                                                                                                    <  1:465202/250‑1 (MQ=255)                                                                                                                                                tGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGa                                                                                                  <  1:159484/250‑1 (MQ=255)                                                                                                                                                  aCGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTACCTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACCCAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGCTTATGAcgc                                                                                               >  1:250907/1‑251 (MQ=255)                                                                                                                                                       cAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCa                                                                                           >  1:523093/1‑250 (MQ=255)                                                                                                                                                           cTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGCAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCCCTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTCACGGATTATGACGCCGCAACggg                                                                                      >  2:320442/1‑250 (MQ=255)                                                                                                                                                            tGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGTAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACt                                                                                                                                                  >  2:187221/1‑190 (MQ=255)                                                                                                                                                            tGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGTAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACt                                                                                                                                                  <  1:187221/190‑1 (MQ=255)                                                                                                                                                               cgccgcgtcggtgcTGT‑CCGCAGGACAGCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGcc                                                                                  <  2:269907/238‑1 (MQ=255)                                                                                                                                                                gccgcTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATCCTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCg                                                                                 >  2:281475/1‑251 (MQ=255)                                                                                                                                                                   gctccgggacggT‑CCGCAGGCGAGCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGGGCCGCGTTCGAAACTGGCCGCTGACCTGAATGCTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCGATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGAGTATGACGCCGCAACGGTAGCCGTgg                                                                              <  2:132405/240‑1 (MQ=255)                                                                                                                                                                      ccgggACTGG‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACGTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGc                                                                            <  2:53674/246‑1 (MQ=255)                                                                                                                                                                           ccTGT‑CCGCAGGACAGCATGCCGTTAACTGAGCTGTTCGGCGCCCGCTTTGCCGACGAGCAGGGCGGGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCTGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGAt                                                                      <  2:143016/250‑1 (MQ=255)                                                                                                                                                                            cTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATg                                                                     >  2:14276/1‑251 (MQ=255)                                                                                                                                                                                   gCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGGTAAAGAACGCTATCTATCGCGCACTGACACAACGGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACTGTATCCGTGGGCGAAGATGCGATgg                                                               <  2:585419/251‑1 (MQ=255)                                                                                                                                                                                     aGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGaa                                                             <  1:199371/251‑1 (MQ=255)                                                                                                                                                                                      ggACTTCATTCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTGCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAg                                                            <  2:411783/251‑1 (MQ=255)                                                                                                                                                                                       gACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAgg                                                           <  1:112987/251‑1 (MQ=255)                                                                                                                                                                                               gCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGTTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTg                                                    <  1:57529/250‑1 (MQ=255)                                                                                                                                                                                               gCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTgg                                                   <  2:114958/251‑1 (MQ=255)                                                                                                                                                                                                ccggtAACTGAGCTGTTCGGCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCTCTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTAGGACGCGGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGa                                                  <  1:516474/247‑1 (MQ=255)                                                                                                                                                                                                         taGCTGTTCGACACCCGCATAACCGACGACCAGAGCGTGCCGCGTTCGAAACTGACCGCTGCCCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATCTGCGGCAGTTAAAGAACGCCATCTCTCGCGCACTGCCACAACAAGACGGGTATGCGCTGCGTCCACAGGCTATTTTGTTGCCGGATTAGGACGCCGCAACGGTAGACGTGGGCGAAGCTGCGCAGGAAGGTTCGCGGGACGAAAacac                                         >  2:272384/2‑251 (MQ=255)                                                                                                                                                                                                             ggTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAAGGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAg                                      <  2:321325/249‑1 (MQ=255)                                                                                                                                                                                                                tcgtcgCCCGCTTGGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCg                                   <  2:472049/250‑1 (MQ=255)                                                                                                                                                                                                                gcttcgCCCGCTTTGCCGACGAGCAGGGCGGGCCGCGTTCTAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGATCGCTATCTATCGCGCACTGACACACCTGGACGGTTATGAGCGGCGTCCACAGGATATGTTGTTGCCGGATTATGACGCCGCAAGGGTAGCCGTGGGCGAAGATGCGATGTAAGGTTCGCTGGACGAAATCACCAGCCGt                                  <  2:384679/248‑1 (MQ=255)                                                                                                                                                                                                                 cgtcgCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGt                                  <  2:128413/250‑1 (MQ=255)                                                                                                                                                                                                                    cgCCCGCTTTGCCGACGAGCTGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGtttt                               <  2:471809/250‑1 (MQ=255)                                                                                                                                                                                                                    cgCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGtttt                               <  1:205573/250‑1 (MQ=255)                                                                                                                                                                                                                       ccGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAAc                           <  1:14276/251‑1 (MQ=255)                                                                                                                                                                                                                                      gCAGGGCGTGCCTCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAAccc            <  1:464462/251‑1 (MQ=255)                                                                                                                                                                                                                                        aGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAg          <  1:448361/251‑1 (MQ=255)                                                                                                                                                                                                                                         gggCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGc         >  2:4896/1‑251 (MQ=255)                                                                                                                                                                                                                                                ccGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATATGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGCCGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGATTTATCg  >  2:277339/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     ATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTGGTCG‑AACTGGTGCAAAAAG‑GCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGT‑CCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCG  >  NC_000913/1387666‑1388152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑