Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,337,394	A→G	S522G (AGC→GGC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,337,394	0	A	G	100.0%	153.8 / NA	47	S522G (AGC→GGC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/20);  total (27/20)

ACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCAC  >  NC_000913/1337149‑1337638                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     aCCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGAt                                                                                                                                                                                                                                                 >  1:286452/1‑251 (MQ=255)   ccAAGTCTGATGATGGCCGCAGGCTTCCTGCCGAAAAAAGCCGTACCTCTGGGCCTCATGCGGCAACCATGGGTAAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACATTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGCttt                                                                                                                                                                                                                                               >  2:104290/1‑251 (MQ=255)    cAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTa                                                                                                                                                                                                                                              <  1:94174/251‑1 (MQ=255)             tGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcggt                                                                                                                                                                                                                                     >  1:174743/1‑251 (MQ=255)              gATGGCCGCAGGCTTGCTGGCGAAAACGGCAGTAACTCTGGGCCTCATGCGGCACCCATGGGTCAAAGCGTCGCTGGAACCGGGTTCGAAAGTCGTTTCTGCTTATCTGGCAAAAGCGAAACTGAAACCGCATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCCATCAAAAAAGGCGATTTCACCGTcggcg                                                                                                                                                                                                                                    >  2:525650/1‑249 (MQ=255)              gATGGCCGCAGGCTTGCGGGCGTAAAAAGCCGTAACTCGGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATGTATCCGTcggt                                                                                                                                                                                                                                     <  2:70687/250‑1 (MQ=255)                     gCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCt                                                                                                                                                                                                                             >  1:316707/1‑251 (MQ=255)                     gCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCt                                                                                                                                                                                                                             >  1:218362/1‑251 (MQ=255)                      cAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAATCTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTAGTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTg                                                                                                                                                                                                                            <  1:564365/251‑1 (MQ=255)                      aaGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCATCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGGTTCTGATTAGCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCGGTATTGGTAACTCTGGGCCGCGGCCCGATCCTATCTAAACGGCAATCAAAAAAGGCGAGTTAACCGTCGGTGCCGTGCTg                                                                                                                                                                                                                            <  2:374047/250‑1 (MQ=255)                       aGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGACAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAACAAGGCGATTTAACCGTCGGTGCGGTGCTGt                                                                                                                                                                                                                           >  2:546866/1‑251 (MQ=255)                        ggCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTc                                                                                                                                                                                                                          <  1:62897/251‑1 (MQ=255)                             gCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCa                                                                                                                                                                                                                     <  2:432225/251‑1 (MQ=255)                                  cgaaaaccgcagtacctctgccccTCAAGCGGGAACCTTGGGGCAACGCGCCGCTGGCACAGGGTTCGACAGTCGTTTCTGCTTATCTGGCAAAAGCGAAACTGACACCGTATCTCACCGCACTGGGGTTTAACCTTGCGGGATACGGTTGTAACACCTGACTTGGTAACTCTCGGCCGCTGCCAGATCCTATCGAAACGGCCATCAACAAAGGCGATTTAAACGACGGTGCGGCGCTGTCCGGCAACCgc                                                                                                                                                                                                                >  2:556782/23‑250 (MQ=255)                                                     gggCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTa                                                                                                                                                                                                <  2:218362/248‑1 (MQ=255)                                                            aaGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCt                                                                                                                                                                                      <  1:230695/251‑1 (MQ=255)                                                               cGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATACGCTGGt                                                                                                                                                                                   >  1:353705/1‑251 (MQ=255)                                                                      aaTGGGTCAAAGCGTCGCTGGAACCGGGTTCGAAAGTCGTTTCTGCTTATCTGGCAAAAGCGAAACTGATACCGTATCTCGACGACCTGGGGCTTACCCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCACGATCCTATAGAAACGGCACTCAAACAAGGCGATTTAACCGACGGTGAGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTACaact                                                                                                                                                                            >  1:348023/2‑251 (MQ=255)                                                                                  cGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGcc                                                                                                                                                                >  1:286419/1‑251 (MQ=255)                                                                                  cGTCGCTGGCACCGGGTTCGAAAGTCGTTTATGATTATATGGCCAAAGCGAAACTGACCCCGTATCTCGCAGAACTGGGGTTTAACATTGTGGGATACGGTTGTAACACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGCTTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGcc                                                                                                                                                                >  2:240402/1‑251 (MQ=255)                                                                                           cACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTcgccgccg                                                                                                                                                       >  2:306916/1‑251 (MQ=255)                                                                                           cACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGGCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTcgccgccg                                                                                                                                                       <  2:333690/251‑1 (MQ=255)                                                                                               ggggtcgcaagtctgttCTTATTATCTGGCAAAAGGTATACGGACACCTTAGCCCGACGAGCTGGGGTTTAACCGTGTGGGGTACGGTTGTTCCACCTGTATAGGTAACTCTGGGCCGCTGCCCGATCCGATCGAAACGGCAATCAAAAAAGGCGATTGAACCGTCGGGGTGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCtcg                                                                                                                                                   <  2:384716/236‑3 (MQ=255)                                                                                                    cGAAAGTCGTTTCTGAATATCTGGCAAAAGCGAAGCTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCAACTGTACTGGTAACTCTGGGCCGCTGCCCGATCCTATCCAGACGGCACTCAAAAAAGGCGATTTAACCGTCGGAGCGGCGCTGTCCGGCAACCGTAACTTTGAAGGCCGTCTCCGTCCGCTGGTTAACACTAACTGGCTGGCCTCGCCGCCGCTGGCGGtt                                                                                                                                              >  2:114854/1‑251 (MQ=255)                                                                                                       aaGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGcc                                                                                                                                           >  1:289011/1‑251 (MQ=255)                                                                                                        aGTCGTTTCTGATTATCTGGCAAAAACGAAACTGACACCGTATATCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGACCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAAACGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCt                                                                                                                                          >  2:494484/1‑251 (MQ=255)                                                                                                                      aTCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATCCGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCACCCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGTCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCggg                                                                                                                             <  2:286419/250‑1 (MQ=255)                                                                                                                            cAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTACCCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGTTTGTTGCCTATGCGCTGGCGGGAAATATg                                                                                                                      <  2:350825/251‑1 (MQ=255)                                                                                                                            cAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCAGCCGCTGGTGGTTGCCTATGCGCTGGCGGGCAATATg                                                                                                                      >  2:42037/1‑251 (MQ=255)                                                                                                                              aaaGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAatatta                                                                                                                     >  2:584081/1‑248 (MQ=255)                                                                                                                                  cGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATc                                                                                                                <  1:306916/251‑1 (MQ=255)                                                                                                                                          acacCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATGCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGc                                                                                                        <  1:521861/251‑1 (MQ=255)                                                                                                                                                tATCTAGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTACAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGACATATGCATATCAACCTGGCttttg                                                                                                   >  2:156118/1‑247 (MQ=255)                                                                                                                                                      gacgaACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTAt                                                                                            >  2:326183/1‑251 (MQ=255)                                                                                                                                                           aCTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGcc                                                                                       >  2:141602/1‑251 (MQ=255)                                                                                                                                                              ggggTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCGCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATg                                                                                    <  1:33237/251‑1 (MQ=255)                                                                                                                                                                     aaCCTTGTGGGAGACGGTTGTACCACCTGTATGGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAGCCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTAGCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCGGGTGGTTGCCTATGCGCTTGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCa                                                                              <  1:336492/250‑1 (MQ=255)                                                                                                                                                                       ccTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAg                                                                           <  2:174743/251‑1 (MQ=255)                                                                                                                                                                                            caccTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAg                                                      <  2:435196/251‑1 (MQ=255)                                                                                                                                                                                                         aaCTCTGGGCCGCTGCCCGATCCTAGCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATc                                         <  2:555943/251‑1 (MQ=255)                                                                                                                                                                                                                         ccGATCCTATCGCAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCACAGGCGATCCGGTTTTTCTGAAAGATATCTGGCCATCGGCACAAAAAATTGcc                         >  2:304664/1‑251 (MQ=255)                                                                                                                                                                                                                           gATCCTATCGAAACGGCAATCAAAAAAGGCGTTTTAACCGTCGGTGAGGTGCTGTCCGGCAACCGTACCTTTGAAGGCCGTATCCATCCGCTGGTTAACACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCACCCTGGCTTCTTAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAACGCTATCTGGCCATCGGCACAAGAAATTGCCCg                       >  1:526741/1‑251 (MQ=255)                                                                                                                                                                                                                                     aaaCGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGa              >  1:101804/1‑250 (MQ=255)                                                                                                                                                                                                                                      caCGGCAGTCAAAAAAGGCGATTTAACCGTCGGTGCGGAGCTGCCCGGCAACCGACACTTTGAAGGCCGTATCCATCAGCTGGATAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGCATATCAACCTGGCTTCTGAGCCTCTCGGCCATGATCGCAAATGCGATCCGGTTTATCTGAAAGATATCTGGCCCTCAGCACAAGCAATTTCCCGGGGGGTAGaa             >  2:381890/2‑250 (MQ=255)                                                                                                                                                                                                                                        cGGCAATCAAAAAAGGCGATTTACCCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCCCAAGAAATTGCCCGTGCGGTAGAACaa          <  2:286452/251‑1 (MQ=255)                                                                                                                                                                                                                                             aTCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACCAGtctc     >  1:372065/1‑251 (MQ=255)                                                                                                                                                                                                                                               cAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTcc    >  1:406424/1‑250 (MQ=255)                                                                                                                                                                                                                                                aaaaaagGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAACACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCCTGAACGCAAAGGCGACCCGGTTTATCTGACAGATATCTGGCCATCGGCACAAAAAATTGACCGAGCGGTAGAACAAGTCTCcac  >  2:539726/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     ACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCAC  >  NC_000913/1337149‑1337638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑