Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,335,123	C→T	intergenic (+294/‑25)	cysB → / → ymiA	DNA‑binding transcriptional dual regulator CysB/uncharacterized protein YmiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,335,123	0	C	T	100.0%	70.5 / NA	22	intergenic (+294/‑25)	cysB/ymiA	DNA‑binding transcriptional dual regulator CysB/uncharacterized protein YmiA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/5);  total (17/5)

ATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGCCAGCATAGTGACAATGAAACAGAAAC  >  NC_000913/1334903‑1335355                                                                                                                                                                                                                             |                                                                                                                                                                                                                                         ataCCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAgg                                                                                                                                                                                                            <  1:557532/251‑1 (MQ=255)  taCCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGt                                                                                                                                                                                                           >  1:34833/1‑251 (MQ=255)                    tATCCATCATATTTATAGGGTACATTACCTGTAAAGTAGTGTCTCATTACCCGCATCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGAGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGa                                                                                                                                                                                          >  2:112412/1‑250 (MQ=255)                     aTCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGaa                                                                                                                                                                                         <  2:194405/250‑1 (MQ=255)                              atTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAAcc                                                                                                                                                                               >  1:317370/1‑251 (MQ=255)                                                   tAAAGAAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTaa                                                                                                                                                           >  2:78424/1‑250 (MQ=255)                                                                                  ccccAGTTTTCAAATATCGATTACAGGTAAAAAGATTAAATTATTCACTGGAGACGATTCGTAAATTCGCTGTATTTTTGACTAAAGTTCCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAAGCGTTCAACTTTTTGCTGATAAGTGATGGCGTTTATATGAGGTTAGCAATGCCTTCTGAAAATCGGGACCCCCGCCGCGATCCTGAATTAAAACGTAAAGTCTGGCTGGCGGTTTTTCTTGGt                                                                                                                           <  2:580851/249‑1 (MQ=255)                                                                                        ttttCAAATATCTATTTCAGTTCAAAAGATTGAATTATTCACTGTACACGATTCGTACTTTCGCTGGATTTTTGACTAAAGTTTCTGTAGGATTTGTCTAATCGTATAATTACTTAAATCTATCGATCAAAGTTTTGCTGTTAAGTGATGGTTTTTATATGAGGTTAGCAATGCCTTCTGGATATAAGGAACCTGGTCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGTCGGTTTTTTTTTGTTCtccc                                                                                                                     >  2:535224/1‑248 (MQ=255)                                                                                                tatCTATTTCAGGTCAAAAGATTGAATTATTAATTGGTGGCGATTCGTAAATTAGCTGGTTTTTTGATTAAAGCTTCTTTAGGCTTTGTCTCATCGTATGATTAATAACACCAATCTTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAATTCAGGAACCCCGCCGCGATCATGAATAAAAACTTAACGCCTGGCTGGCGGGGTTCCTTGGCTGTGCACTTTTCtt                                                                                                             >  2:159380/1‑250 (MQ=255)                                                                                                  tCTATTTCAGGTCAACAGATTGCATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGACTTAAACCGTAAAGACTGGCTGGCGGTTTTTCTTGGTTCTGCCCTTTTCTggg                                                                                                           >  2:56106/1‑251 (MQ=255)                                                                                                            gTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCACTGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGGTCTGCACTTTTCTGGGGGGTTGTCGc                                                                                                 >  2:372398/1‑251 (MQ=255)                                                                                                              cAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCa                                                                                                <  2:245168/250‑1 (MQ=255)                                                                                                                               cACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGATTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAgtg                                                                              >  1:24763/1‑251 (MQ=255)                                                                                                                                     agaCGCTTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTTGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGGGTggggg                                                                        >  1:158478/1‑250 (MQ=255)                                                                                                                                         gATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGGGGTTGTCGACCTGCTGATTTGGAAAGTGTGGGGATAAc                                                                    >  2:430007/1‑251 (MQ=255)                                                                                                                                                     cGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTGGGGATAACTATGGTCGGTCa                                                        <  1:78424/251‑1 (MQ=255)                                                                                                                                                        tGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTGGGGATAACTATGGTCGGTCAggg                                                     >  1:378670/1‑250 (MQ=255)                                                                                                                                                                                            aTCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGCCAGCATAGTg                  >  1:37677/1‑250 (MQ=255)                                                                                                                                                                                             tCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGCCAGCATAGTGAc                >  2:506065/1‑251 (MQ=255)                                                                                                                                                                                                     ttaattaCACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTGGGGGTAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGCCAGCATAGTGACAATGAAAc        >  1:456345/1‑251 (MQ=255)                                                                                                                                                                                                         ttaCACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGCCAGCATAGTGACAATGAAACAga     >  1:226727/1‑250 (MQ=255)                                                                                                                                                                                                           acacCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGCCAGCATAGTGACAATGAAACAgaaac  >  1:313043/1‑251 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                         ATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGCCAGCATAGTGACAATGAAACAGAAAC  >  NC_000913/1334903‑1335355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑