Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	88,571	G→A	V182M (GTG→ATG)	cra →	DNA‑binding transcriptional dual regulator Cra

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	88,571	0	G	A	100.0%	324.8 / NA	100	V182M (GTG→ATG)	cra	DNA‑binding transcriptional dual regulator Cra
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (54/46);  total (54/46)

AGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGGTGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGCTGCGTCGCGACGGC  >  NC_000913/88324‑88813                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   agaagaTCCGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGGGCCGATCAGGATGATGCCGAAATGCTGGCGGAGGAGTTACGTAAGTTTCCC‑GCCGCGACGATGc                                                                                                                                                                                                                                                 >  2:346942/1‑251 (MQ=255)  gaagaTCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTCCAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTGTCCC‑GCCGAGACGATGCt                                                                                                                                                                                                                                                <  1:163651/251‑1 (MQ=255)  gaagaTCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCt                                                                                                                                                                                                                                                >  1:274444/1‑251 (MQ=255)    agaccAGCCAGACAACGAAATGGGGTGAATTGAGCCCCTTTTACGGCGGCGGGTTGGTGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGG‑CTAACGACCGGTC‑CCCGATTGTCGCGCTGGCCCGC‑GCCCTCGATCGGGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGAGGCCGAAATGCTGGCGGAAGAGTTACGTATGTTTCCC‑GCCGAGACGATGCttt                                                                                                                                                                                                                                              <  2:324792/247‑1 (MQ=255)           ccAGACAACGAAATGCGGGGCATTGAGCCCCTTTTACAGCGTCAGGTTGATGCCATTATTGTTCCGACGTCGTTGCCTCCTGAGCATCCTTTTTAGCAGCGCGGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGCCCGC‑GCCCTCGATCGTGAACACTTCCCCAGCGTGGGTGGTGCCTATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTgg                                                                                                                                                                                                                                       <  1:229626/251‑1 (MQ=255)            cAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGt                                                                                                                                                                                                                                      >  2:131247/1‑251 (MQ=255)                               cATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGCGACGATGCTTTATCTTGGTGCGCTACCGGCGCTTTCTg                                                                                                                                                                                                                   >  1:367063/1‑251 (MQ=255)                               cATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTg                                                                                                                                                                                                                   >  2:42055/1‑251 (MQ=255)                                aTTGAGCACCTTTTACAGCGTCAGGTTGCTGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCCTCCTTTTTATCAACGCTGGG‑CTAACGCCCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCCGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGATTCCC‑TCCGCGACGATGCTTTATCTTGGTGCGCTACCGGAGCttaccgt                                                                                                                                                                                                                  >  2:198426/1‑246 (MQ=255)                                    aGCACCTTTTACAGCGTCAGGTTGATGCCATTCTTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGCCGATGCTTTATCTTGGTGCTCTACCGGAGCTTTCTGTCAGc                                                                                                                                                                                                              >  2:66183/1‑251 (MQ=255)                                     gCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGACATGCTGGCGGGAGAGTTACGAAAGTTTCCC‑GCCGTGACGATGCTTTATCTTGGTGTGCTAACGGAGCTTTCTGTCAGCt                                                                                                                                                                                                             >  2:314818/1‑251 (MQ=255)                                     gCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCt                                                                                                                                                                                                             >  1:18678/1‑251 (MQ=255)                                       cccTTTTACCGCGTAAGGTTGATTCCTTTATTGTTTCGACGTCGTTGCCTCCTTAGCATCCTTTTTATCAACGCTGGG‑CTAACGCCCCGTT‑CCCGATTGCCGCGCGGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTTCGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTc                                                                                                                                                                                                           <  1:163403/250‑1 (MQ=255)                                           tttACAGCGTCAGGTTGATGCCATTATTGTTTCGACGCCGTTGCCCCCTGAGCACCCTTTTTATCAACGCTGGG‑CTAACGCCCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTg                                                                                                                                                                                                        <  1:16961/250‑1 (MQ=255)                                             tACAGCGTCAGGTTGATGCCATTATTTTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATAAACGCTGGG‑CTACCGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCg                                                                                                                                                                                                      <  1:447185/250‑1 (MQ=255)                                             tACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCGGGG‑CTAACGCCCCGTT‑CCCGATTGTCGCGCTGGGCCGG‑GCCCTCGATCGTGAACACTTCACCAGCTTGTTTGGTGCGGAGCAGAATGAGGCCGAAAGGCTGGCGGAAGAGTTGCGAAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCg                                                                                                                                                                                                      <  1:338089/250‑1 (MQ=255)                                              aCAGCGTCAGGTTGATGCCTTTTTTGTTTCGAGTTCGTGTCCTCTTGAGCGTCCTTTTTATCAACGCTGGC‑CTAACGGCCCGTT‑CCCGATTGTCCCCCTGGTCCGC‑CCCCTCTATCGTGAACACTTCACCAGCGTGGGTGGTGCCGAACAGGATGATGCCAAAATTCTGGCGGAAGAGTGACTTAGGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCGACCGGAGCTTTCTGTCAGCTTCCTGCGTg                                                                                                                                                                                                    <  1:494657/251‑1 (MQ=255)                                               cAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCGGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGACCACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTg                                                                                                                                                                                                    >  1:222059/1‑250 (MQ=255)                                                  cGTCAGGTTGATGCCATTATTGTTTCGCCGTCGTTGCCTCCTGAGCATCCTTTTTACCAACGCTGGG‑CTAACGCCCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCCCCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACa                                                                                                                                                                                                <  1:396545/251‑1 (MQ=255)                                                  cGTCAGGTTGATGCCATTATTGTTTCGACGTCGATGCCTCCTGAGCATCCTTTTTATCAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGCGACGATTCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTgcac                                                                                                                                                                                                 >  2:130559/1‑247 (MQ=255)                                                     cAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATc                                                                                                                                                                                                                                           >  1:582301/1‑205 (MQ=255)                                                          tGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTcc                                                                                                                                                                                        >  1:292708/1‑251 (MQ=255)                                                           gATGCCATTATTGTTTCGACGTCGTTGCCTCCTGCGCATCCTTTTTATCAAAGCTGGG‑CTAACGATCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAGCACTTCACCAGCGTGGTTGGTGCCGATCAGGCTGAGGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGGGCGCAACCGGAGCTTTCTGTCAGCTTCATGCGTGAACAAGGTTTCCg                                                                                                                                                                                       >  2:95589/1‑251 (MQ=255)                                                             ggCCATTTTTGTTTCGACGTCTTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGG‑CTAACGACCCGTC‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCTATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGTATGATGCCGAAATGCTGGCGGAAGAGTTACGTAATTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGt                                                                                                                                                                                      <  1:432419/249‑1 (MQ=255)                                                              gtcATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCAGAAATGCGGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTAc                                                                                                                                                                                    <  1:125308/249‑1 (MQ=255)                                                                 attattGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGG‑CTACCGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGc                                                                                                                                                                                 >  2:330251/1‑251 (MQ=255)                                                                       gTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGACCACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGACGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGa                                                                                                                                                                            >  1:565210/1‑250 (MQ=255)                                                                         ttCGACGTCGTTGCCCCCTGAGCATCCTTTTTATCAACGCGGGG‑CTAACGCCCCGTT‑CCGGATTGTCGCGCTGGCCCGC‑GCCCTCGATCGTGAACACTCCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGaaa                                                                                                                                                                          <  1:472521/250‑1 (MQ=255)                                                                          tCGACGTCGTTGCCTCCTGAGCTCCCTTTTTATCAACGCTGGG‑CTAACGCCCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCCCCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTTGAAAg                                                                                                                                                                         <  2:123750/250‑1 (MQ=255)                                                                            gACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAgatg                                                                                                                                                                      >  1:17137/1‑251 (MQ=255)                                                                               gtcgtTGCCTCCTGAGCATCCTTTTTATCCACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCCGCGTGGTTGGTGCCGATCAGGCTGATGCCGAAATGCTGGCGGAAGAGGTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGCAAGATGATc                                                                                                                                                                   >  2:495476/1‑251 (MQ=255)                                                                               ggcgtTGCCTCCTGAGCATCCTTTTTATCAACGCTGGG‑CTAACGACCCGTT‑CCCGCTTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGGTGATGCCGAAATGCTGGCGGCAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTCCCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTGCCGTACTGCCTGGACAgatgctc                                                                                                                                                                   >  2:432422/3‑248 (MQ=255)                                                                                  gtTGCCTCCTGAGCATCCTTTTTATCAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTcc                                                                                                                                                                                                          >  1:483845/1‑209 (MQ=255)                                                                                   ttGCCTCCTGAGCATCCTTTTTATAAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCACTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGCTGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCcgcg                                                                                                                                                               >  2:295633/1‑251 (MQ=255)                                                                                   ttGCCCCCTGCGCATCTTTTTTACCAACGCGGGG‑CTAACGCCCCGTT‑CCGCATGGTCGGGCTGGACCGC‑GCCCCCGATCGGGAACCCTTCCCCAGCGTGGTGGGGGCCGATCAGGATGATGCCGAAAGGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCcgc                                                                                                                                                                <  2:380358/250‑1 (MQ=255)                                                                                    ggccccctGAGCACTCTTTTTAT‑AACCGTGGGTCTAACGACCCGGT‑CCCGATTGGGGCGCGGGCCCGC‑GCCCTCGATGGAGAACACTTCACCAGGGTGTTTGGTGCGGATCAGGATGAGGCCGAAATGCTGGTGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCGTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGGTTCCGTACTGCCTGGAAAGATGATCcgcg                                                                                                                                                               <  2:483280/245‑1 (MQ=255)                                                                                     gCCTCCTGAGAATCCTTTTTATCAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCcgcgc                                                                                                                                                              <  1:185639/250‑1 (MQ=255)                                                                                        tcctGAGCATCCTTTTTATCAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGa                                                                                                                                                            >  1:81086/1‑249 (MQ=255)                                                                                            gggCATCCTTTTTACCAACGCGGGG‑CTAACGCCCCGTT‑CCCGATTGTCGCGCGGGCCCGC‑GCCCGCTATCGTGAACACTTCCCCAGGTTGGTTGGGCCCGAGCAGGATGGTGCCAAAATGCTGGCGGAAGAGTGACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACGGCCTGGAAAGAGGATCCGCGCGAAGTg                                                                                                                                                        <  2:339546/247‑1 (MQ=255)                                                                                              gCATCTTTTTTATCAACGCGGGT‑ATAACGACCCTTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACCCTTCACCAGCGTGGTTGGTGCCGATCAGGATGATCCCAAAATGCTGGCGGAAGATTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCTCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCAtt                                                                                                                                                    <  1:283655/251‑1 (MQ=255)                                                                                                     tgttATCAACGCTGGG‑CTAACGCCCCGTT‑CCCGATTGTCGCGGTGGACCGC‑GCCCGGGTGCGTGAACACTCCACCAGCGTGGTTGGTGCCGATCAGGAGGATGCCGAAATGCGGGCGGAAGAGTTAGGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGGTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGt                                                                                                                                              <  1:363130/248‑1 (MQ=255)                                                                                                       ttATCAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGCCCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGGGCCGATCAGGATGATGCCGAAATGCTGGCGGCAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATg                                                                                                                                           >  2:433221/1‑251 (MQ=255)                                                                                                           cAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCa                                                                                                                                        >  1:129094/1‑250 (MQ=255)                                                                                                           cAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAAAAAGGTTTCCGTACTGCCTGGAAAGCTGATCCGCGCGAAGTGCATTTCCTGTATGCCa                                                                                                                                        >  2:345281/1‑250 (MQ=255)                                                                                                            aaCGCGGGG‑CTAGCGCCCCGTT‑CCCGATTGTCGCGCGGGCCCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGTTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTA‑GTTTCCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAAc                                                                                                                                      <  2:222059/251‑1 (MQ=255)                                                                                                               gcgggg‑CTAGCGCCCCGTC‑CCCGTTTGTCGGGCTGG‑CCGCCTCCCGCGATCGGGACCACTTCCCCACCTTGGTGGGCGCCTATCAGGAGTTTGCCGAAATGCTGGCGGAAGAGTTCCGTAATTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATCATCCGCGCGAAGTGCATTTCCTGTAGGCCAACAGc                                                                                                                                   <  2:377757/248‑1 (MQ=255)                                                                                                               ccTGGG‑CTAA‑GGCCCGTTTCCCGTTTGCCGCGCTGGCCCGC‑GCCCCCGATCGTAAACATTTCACCAGCGTGGTTGGTGCCGACCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGGAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGc                                                                                                                                   <  2:340968/250‑1 (MQ=255)                                                                                                                cTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGCACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGCTCAGGATGATGCCGAAATGCTGGCGGAAGCGTTCCGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCCTGCACAAGGTTTCCGTACTGCCTGGAACGATGATCCGCGCGCAGTGCATGTCCTGTATGCCAACAGCt                                                                                                                                  >  2:374023/1‑251 (MQ=255)                                                                                                                  ggg‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGACCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTa                                                                                                                                 <  1:16029/250‑1 (MQ=255)                                                                                                                       tAACGACCCGAT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGACATGCTGACGGAAGAGTTACGTAAGTTTCCC‑GCCGAGTCGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGACGTGCATTTCCTGAATGCCAACAGCTATGAGc                                                                                                                            >  2:400430/1‑251 (MQ=255)                                                                                                                        aaCGACCGGTT‑CCCGATTGCCGCGCGGGACCGC‑GCCCTCGATCGGGAACACTCCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCGGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGc                                                                                                                            <  1:77832/250‑1 (MQ=255)                                                                                                                           gACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCCGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGCAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCgggg                                                                                                                        >  2:364243/1‑250 (MQ=255)                                                                                                                           gACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGa                                                                                                                              >  1:13472/1‑245 (MQ=255)                                                                                                                           gACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCggg                                                                                                                         >  1:181059/1‑250 (MQ=255)                                                                                                                           gACCCGTC‑CCCGATTGCCGCGCGGGACCGC‑GCCCTCGATCGGGAACACTTCACCAGCGGGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGa                                                                                                                              <  2:13472/245‑1 (MQ=255)                                                                                                                            ccccttt‑CCCGTTTGCCGCGTTGGCCCGC‑GCCCGCAGCCGTGAACTCTTCACCAGCGTGGTTGGTGCCGATCAGGATGCTGCCGAATTTCTGGCGGAAGAGTTACGTATGTTCCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCGATGAGCGCGAg                                                                                                                       <  2:383345/246‑1 (MQ=255)                                                                                                                            ccccGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAg                                                                                                                       <  1:42055/250‑1 (MQ=255)                                                                                                                              ccGTT‑CCCGATTGTCGCGCTGGCCCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCTGGAggc                                                                                                                     <  1:438455/251‑1 (MQ=255)                                                                                                                              ccGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACCCTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGCTCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGGggc                                                                                                                     >  2:378519/1‑251 (MQ=255)                                                                                                                              ccGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGAGGACGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGATCTTTCTGTCAGCTTCCTGCGTGCACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTCTGAGCGGGAggc                                                                                                                     >  2:215759/1‑251 (MQ=255)                                                                                                                               cGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAggcg                                                                                                                    >  2:459500/1‑251 (MQ=255)                                                                                                                                  t‑CCCGATTGTCGCGCTGGAACGC‑GCCCTCGCTCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGATGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTCTGCCAACAGCTATGAGCGGGCGGCGGCt                                                                                                                 >  2:551/1‑251 (MQ=255)                                                                                                                                           gccgcgtgggcccGC‑GCCCCCGATGGTTAACACTGTACCAGTGTGTGTGGTGCTGTTCAGGAGGATCCGGAATTGTGGGGGGAAGAGTTACGTAAGGTTACC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCTCAGtt                                                                                                         <  2:309137/240‑1 (MQ=255)                                                                                                                                             cgcgCTGGACCGC‑GCCCTCGATCGTGAACCTTTCCCCAGGGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTa                                                                                                        <  1:390827/250‑1 (MQ=255)                                                                                                                                              gcgcTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTAtt                                                                                                      >  1:198789/1‑251 (MQ=255)                                                                                                                                               cgcTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTc                                                                                                     <  1:217162/251‑1 (MQ=255)                                                                                                                                                         c‑gcCCTCGATCGGGAGCGCCTAACCAGCGCGGTCGCAGCCGTTCAGGACCATGCCGACATGCTGGCGGAAGACATACGTAAGTTTCCC‑ACCGTGACGATGATTTACCTTGGTGCGATCCCGGAGGTTTCTGACCGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGGTCAGCGCGCAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATACGAAAAATGGc                                                                                           >  2:302237/1‑251 (MQ=255)                                                                                                                                                           gcCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCACTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCt                                                                                          >  2:485543/1‑251 (MQ=255)                                                                                                                                                            cccTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTg                                                                                         >  1:325333/1‑251 (MQ=255)                                                                                                                                                                cGATCGTGACCACTTTCCCAGCGGGGTTGGTGCCAATCAGGTTGTTGCCGAAAGTCTGGCGAAAGATTGACGTAATTTGCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGaa                                                                                      <  1:517825/250‑1 (MQ=255)                                                                                                                                                                 gATCGTGAACACTTCCCCAGCGGGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACTTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCt                                                                                                                                                <  2:125246/191‑1 (MQ=255)                                                                                                                                                                 gATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCt                                                                                                                                                >  1:125246/1‑191 (MQ=255)                                                                                                                                                                     gTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCt                                                                                                                 >  1:21864/1‑218 (MQ=255)                                                                                                                                                                      tGAACACTTCACCAGCGTGGTTGATGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGGTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAACATGGCTGGGAACGCATc                                                                               >  2:377035/1‑251 (MQ=255)                                                                                                                                                                       gAACACTTCACCAGCGTGGTTGGGGCCGATAGGGATGAGGCCGAAAGGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATcc                                                                              <  2:178238/251‑1 (MQ=255)                                                                                                                                                                        aaCACATCACCGGCGGGGTTGGGGCCGAGAAGGATGAGGCCGAAAGGTTGGCGGAAGAGTTAGGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGAGCCGCGCGAAGTGCATTTCTTGTATGCCAACAGCTATGTGCGGGAGTCGGCTGCCCAGTTATGCGAAAAATGGCTGGAAACGCATCCg                                                                             <  2:306385/251‑1 (MQ=255)                                                                                                                                                                               cACCAGCGGGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTCCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCg                                                                       <  1:138731/250‑1 (MQ=255)                                                                                                                                                                                   aGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGgc                                                                  >  2:400442/1‑251 (MQ=255)                                                                                                                                                                                       tggttggtGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTg                                                              >  2:546441/1‑251 (MQ=255)                                                                                                                                                                                        gtttggtGCCTAACAGGAGGATGGCGAAATTCTGGCGGAAGAGTTATGTAAGTTTCCC‑GCCGAGACGATTCGTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATTCCAACAGCTATGATCGGGAGGCGGCTGCCCAGTTATTCGAACAATTGCTGGAAACGCATCCGATGCCGCAGTCGCTGt                                                             <  2:365334/249‑1 (MQ=255)                                                                                                                                                                                        ggttggtGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGt                                                             >  1:172678/1‑251 (MQ=255)                                                                                                                                                                                              ggCCGACCAGGATGAGGGCGAATGGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACaa                                                       <  2:391473/250‑1 (MQ=255)                                                                                                                                                                                                 cGATCAGGATGATGCCGAAATGCTGGCGGAAGTGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACAGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGAATCCGATGCCGCAGGCGCTGTTCACAAcgt                                                    >  2:563026/1‑251 (MQ=255)                                                                                                                                                                                                 cGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAAcgt                                                    >  1:4703/1‑251 (MQ=255)                                                                                                                                                                                                     cAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCCTCCGCTGCCGCAGGCGCTGTTCACAACGTCGtt                                                >  2:463849/1‑251 (MQ=255)                                                                                                                                                                                                      aGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGttt                                               >  1:372468/1‑251 (MQ=255)                                                                                                                                                                                                      aGGATGATGCCAAAAGGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGttt                                               <  1:76208/251‑1 (MQ=255)                                                                                                                                                                                                           gatgCCGAAAGGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCgtt                                          <  1:131247/251‑1 (MQ=255)                                                                                                                                                                                                              gCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCgttgtt                                       <  1:185177/251‑1 (MQ=255)                                                                                                                                                                                                                 aaaaggCTGGCGGAAGAGTGACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCa                                    <  1:346942/246‑1 (MQ=255)                                                                                                                                                                                                                    aTGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAgg                                 >  1:129669/1‑251 (MQ=255)                                                                                                                                                                                                                        ggcgtaaatagtgacatAAGTTTCCC‑GCCGTGACGATGCTTTACCTTGGTGCGCTACCGGAGCTTTCTGGCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCCTTTCCCGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCTGAAGCTGCAGGCGCTGTTCACAACGTCGTTTGCGGTGTTGTAAGGAGTg                             <  2:323091/235‑1 (MQ=255)                                                                                                                                                                                                                         ggCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGt                              <  1:105970/249‑1 (MQ=255)                                                                                                                                                                                                                           gggAAGAGTTATGTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTgatg                          <  2:181059/250‑1 (MQ=255)                                                                                                                                                                                                                                       gTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGCAAGATGATCCGCGCGCAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGCAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGCTg              >  2:156433/1‑251 (MQ=255)                                                                                                                                                                                                                                       gTAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGCTg              >  2:456551/1‑251 (MQ=255)                                                                                                                                                                                                                                        tAAGTTTCCC‑GCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGCTGc             >  1:446792/1‑251 (MQ=255)                                                                                                                                                                                                                                                 c‑gccgAGACGATGCTTTATCTTGGTGCGCTACCGGAGATTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGTCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGCTGCGTCGCGACg    <  2:450277/251‑1 (MQ=255)                                                                                                                                                                                                                                                   gccgAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGATGCGTCGCGACgg   >  1:394914/1‑251 (MQ=255)                                                                                                                                                                                                                                                     cgAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATACGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGCTGCGTCGCGCCGGc  >  2:560381/1‑250 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   AGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGG‑CTAACGACCCGTT‑CCCGATTGTCGCGCTGGACCGC‑GCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCC‑GCCGAGACGGTGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGCTGCGTCGCGACGGC  >  NC_000913/88324‑88813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑