Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,297,496	G→A	A209V (GCT→GTT)	adhE ←	fused acetaldehyde‑CoA dehydrogenase and iron‑dependent alcohol dehydrogenasealdehyde/alcohol dehydrogenase AdhE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,297,496	0	G	A	97.9%	145.1 / NA	47	A209V (GCT→GTT)	adhE	fused acetaldehyde‑CoA dehydrogenase and iron‑dependent alcohol dehydrogenasealdehyde/alcohol dehydrogenase AdhE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (27/19);  minor base C (1/0);  total (28/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGAGAAGATAATGGCGTTACGGGTCTTC  >  NC_000913/1297255‑1297738                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   gTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGCTAACTGGTTTAc                                                                                                                                                                                                                                           >  1:4108/1‑251 (MQ=255)       cAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGc                                                                                                                                                                                                                                     <  1:84660/251‑1 (MQ=255)             aaCATCCGGAACAGCTTTCAGCTCTTTGCCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGGCGAAGGTTTTGGACATCAGTACAGATGCAACGGCACGGTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACGCCGATAACTGGTTTACCGGAGCTGTATg                                                                                                                                                                                                                               <  2:354130/251‑1 (MQ=255)              aCATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCACGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGc                                                                                                                                                                                                                              >  2:213074/1‑251 (MQ=255)                     gAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCttt                                                                                                                                                                                                                        <  1:143386/250‑1 (MQ=255)                              tCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCAAGTACAGCGTCATAAACGGCGACAACAACAACAACAGCCTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGAGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGccc                                                                                                                                                                                                              >  1:237982/1‑251 (MQ=255)                                   tctTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTCCGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGacc                                                                                                                                                                                                         >  1:357255/1‑251 (MQ=255)                                          ccTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTc                                                                                                                                                                                                  >  2:187189/1‑251 (MQ=255)                                                  aGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCATGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGCCCAACAGTCGCGAgg                                                                                                                                                                                            >  1:257585/1‑249 (MQ=255)                                                          gccgTGGGCTGCAAAACGTTCACGTACAGCGTCATAAACCGCGTCCACAACCACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTg                                                                                                                                                                                   >  2:287039/1‑250 (MQ=255)                                                                      aaaaCGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGggtggt                                                                                                                                                                      >  2:40882/1‑251 (MQ=255)                                                                         aCGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGc                                                                                                                                                                    >  1:587594/1‑250 (MQ=255)                                                                           gTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATACCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATc                                                                                                                                                                 >  2:311457/1‑251 (MQ=255)                                                                               aCGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTGTTGGACATCAGTACAGATGCAACTGCACGTTTGATAACAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACTCCGATAACTGGTTTACCGGAGCTGTAGGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTTATGTCTGGGTGGTGCATCAGTg                                                                                                                                                             <  2:237019/251‑1 (MQ=255)                                                                                         tCATAAACAGAGTCAACAACAACCACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCCGGTTGTTGTCTGGGTGGTGCATCAGTGCGTTAGAc                                                                                                                                                     >  2:119469/1‑249 (MQ=255)                                                                                              aaCAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGCGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGAGCGTTAGACAGTTCa                                                                                                                                               >  2:120992/1‑250 (MQ=255)                                                                                              aaCAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCAGTTGTCGAAGGCTTTGGACATCAGTAAAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGACAACAACTGGAGAGTTGCCCGCGCCTCCACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTAAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCa                                                                                                                                               >  1:76619/1‑250 (MQ=255)                                                                                                cAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCTATAACAACGGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTATCCATGGCCGGACCACCAGTCGCGAGGTTCATTTTGATGTCTGCGTGGTGCATCAGTGTGTTAGTCAGTTCAACa                                                                                                                                            <  1:542742/251‑1 (MQ=255)                                                                                                 agagTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGAt                                                                                                                                                                                 <  1:348738/213‑1 (MQ=255)                                                                                                      caacaacaacaaCAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCAGCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAgg                                                                                                                                       <  2:587594/250‑1 (MQ=255)                                                                                                                   aGACTGTTCATAAGCACAGATTACGCCGGTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGCTAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGAGGTCTGGGTGGTGCGTCAGTGCGTTAGACAGTTCAACAGAAGGATAATCGATCCAGc                                                                                                                         >  2:317820/1‑251 (MQ=255)                                                                                                                        tttaaGAAGCCCAGATTATGCCGTTTTCGAAGGTTTTGGACATCAGTACAGATGCAACTCCCCGTTGGATTTCAGCAGTTTCATCGGTAACAAGGGGAGTGTTGCCCGCGCCTACACCTAGAACTGGTTTACCGGAGCTGTATGCGTCTTTAACCATGCCGGGACCACGAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTTCATCAGTGCGTTGGACTGTGGAACAGAAGGGTTATCTATCCAGCCGAt                                                                                                                     <  2:586307/246‑1 (MQ=255)                                                                                                                           cAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGa                                                                                                                 <  1:555142/251‑1 (MQ=255)                                                                                                                            aGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCACGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGAt                                                                                                                <  1:488415/251‑1 (MQ=255)                                                                                                                              aaGCACAGGTTACGCCGTTATCGGAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAGCTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCACGAGCCACCAGTCGCGAGGATCAGGTTGATGTCTGTGTGGTGCATCAGTGCGTTAGACAGTTCAAAAGAAGGTTGATCGATCCAGCCGATCCGATCt                                                                                                              >  2:116051/1‑251 (MQ=255)                                                                                                                                           gCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCgg                                                                                                 >  1:226869/1‑251 (MQ=255)                                                                                                                                                tGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGc                                                                                             <  1:190212/250‑1 (MQ=255)                                                                                                                                                 gTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTGAACCATGCCCGGACCACCAGTCGCGAGGAGCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCTGCAGCg                                                                                            <  2:273431/250‑1 (MQ=255)                                                                                                                                                       ggTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATagc                                                                                       >  1:204907/1‑249 (MQ=255)                                                                                                                                                             ggACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGc                                                                                >  1:32758/1‑250 (MQ=255)                                                                                                                                                             ggACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGc                                                                                <  1:187189/250‑1 (MQ=255)                                                                                                                                                                     gTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGTTCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGat                                                                        >  2:493917/1‑250 (MQ=255)                                                                                                                                                                           aTGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGAGCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGcc                                                                 <  1:213074/251‑1 (MQ=255)                                                                                                                                                                              cAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTCTGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCCTCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCt                                                              >  1:380275/1‑251 (MQ=255)                                                                                                                                                                                       gTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTtggtg                                                     <  1:311457/251‑1 (MQ=255)                                                                                                                                                                                       gTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTtggtg                                                     <  1:287039/251‑1 (MQ=255)                                                                                                                                                                                        tttGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGATGATGTCTGGGTGGTGCCTCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTtggtgg                                                    >  2:87070/1‑251 (MQ=255)                                                                                                                                                                                         ttGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCAGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCCGAACGATATCAGCCGATTTGTTGGTGGc                                                   >  2:17021/1‑251 (MQ=255)                                                                                                                                                                                           gATATCAGCAGTTTCATCGATAACAACTGGAGTGTCGCCCGAGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGGGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCAt                                                 >  1:163362/1‑251 (MQ=255)                                                                                                                                                                                                   cagTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGc                                          >  1:19126/1‑250 (MQ=255)                                                                                                                                                                                                    agTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCAc                                        >  1:450590/1‑251 (MQ=255)                                                                                                                                                                                                      tttCATCGATAGGAAATGGATTGTTGCCCGAACCCACACAGATAACACGTTTACAGGAGCTGTATGCGACTTTAACCATGCCCCGACAACCAGTCGCGAGGATCAGGCTGATGTCTGGCTGGTGCATCAGTACGTTAGACAGTTCAAAAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCCGCCTGCAGAACGATATAAGACGCTTTGTTGGTGGCATCTTATGCAcgc                                      >  2:65456/1‑251 (MQ=255)                                                                                                                                                                                                         cATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCggg                                   >  2:358119/1‑251 (MQ=255)                                                                                                                                                                                                                              tGCCCGCGCCGACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTGTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGGCTGGGTGGTTCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGAGAAGATAATGGc               <  2:357255/250‑1 (MQ=255)                                                                                                                                                                                                                                    cgcCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGAGAAGATAATGGCGTTACgg        >  2:228358/1‑251 (MQ=255)                                                                                                                                                                                                                                          cacCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGAGAAGATAATGGCGTTACGGGTCTTc  <  2:424894/251‑1 (MQ=255)                                                                                                                                                                                                                                          cacCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGAGAAGATAATGGCGTTACGGGTCTTc  <  1:40882/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   GTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATCAGCCGCTTTGTTGGTGGCATCTTTTGCACGCGGGTGCGGGGAGAAGATAATGGCGTTACGGGTCTTC  >  NC_000913/1297255‑1297738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑