Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,269,702	C→T	intergenic (+313/+1)	rdlA → / ← ldrB	putative antisense regulatory RNA RdlA/small toxic polypeptide LdrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,269,702	0	C	T	100.0%	87.0 / NA	32	intergenic (+313/+1)	rdlA/ldrB	putative antisense regulatory RNA RdlA/small toxic polypeptide LdrB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/12);  total (20/12)

ATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACC  >  NC_000913/1269466‑1269945                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    aTACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTAc                                                                                                                                                                                                                                       >  1:421906/1‑251 (MQ=255)                                  tGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGCCAATCGCTGCGGTAATAATTc                                                                                                                                                                                                      >  1:20194/1‑251 (MQ=255)                                         tttGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGcc                                                                                                                                                                                                 >  2:58802/1‑249 (MQ=255)                                                tGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGt                                                                                                                                                                                        >  2:286377/1‑251 (MQ=255)                                                       tGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCCGCCTGACCCCCGTTTTCACTTTACTTCCGGTTACACCACCAGCCGCCAATCGCTGCGGTAATAATTACCGCCAGGATCGGTgccgccc                                                                                                                                                                                 >  1:31910/1‑250 (MQ=255)                                                         cATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAgg                                                                                                                                                                               >  1:545746/1‑251 (MQ=255)                                                                      cAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTGGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTTCCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGaaa                                                                                                                                                                   <  1:271337/250‑1 (MQ=255)                                                                                tACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGACGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGAATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCGGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGCTCGGTGCCGCCAGTCCGTTCCAGaacaacatggaaa                                                                                                                                                         >  2:435654/1‑239 (MQ=11)                                                                                                   cgcgggATGAGGCTTCCAGCCCGGGTTGCCAATATTTACTTGTGGAAGGGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCCCCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAg                                                                                                                                     <  2:11109/247‑1 (MQ=255)                                                                                                      tGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGGAGGCTATTACCGCTGCGGAGGGATTGAGGCGAATTTTGCGATCACGGCAGCCTGTCGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCACAATGCGCGAGCGTCATATCgccg                                                                                                                                  >  2:246331/1‑251 (MQ=14)                                                                                                                     gCTCGGGTTGCCAATATTTACTTGTGGAAGGGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCCCCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGac                                                                                                                   <  1:226142/251‑1 (MQ=21)                                                                                                                        cGGGTTGCTAATATTTACTGGTGGAAGTGATAAAGACAAAAAGGGCCGCAGGCTGTTCCCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGGCGCCCGTTTTCACTTTACTTCCGGTTACGCCCCCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGacaac                                                                                                                <  1:175144/251‑1 (MQ=11)                                                                                                                                             tgGAAGTGCTAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCATGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGACTTGTTGTAATGACAACATTTTGCGGCTATTCTTGa                                                                                             >  1:189361/1‑249 (MQ=255)                                                                                                                                                aaGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAgt                                                                                          >  1:239506/1‑249 (MQ=37)                                                                                                                                                aaGTGAAAAAGACAAAAAGGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGGTGTAATGACAACATTTTGCGGCTATTCTTGAAgt                                                                                          <  2:239506/249‑1 (MQ=255)                                                                                                                                                   tGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTg                                                                                     >  1:435480/1‑251 (MQ=25)                                                                                                                                                     aTAAAGACAAAAATGGCAGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAAAATTTTGCGGCTATTCTTGAAGTGTCTGGt                                                                                   >  2:270597/1‑251 (MQ=21)                                                                                                                                                       aaaGACAAAAACGGCTGAAGGCTTTTACCCCTGCGGCCGTTTTCGGGCGCATATTGCCATCACGGCACCCTGACGCCCGTTTCCACTTTACTTCCGGTTACGCCCCCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGttt                                                                                 <  1:286377/251‑1 (MQ=11)                                                                                                                                                                     ccGCAGGATGTTACACCTGAGTCCGGTTTTGGGCGCATATTGCCATAACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGCGCCGCAAGGTCGTGCCAGAAAATCACGGCAAACGGCGCGAGCGTCATATAGCAGCCTTGTTGTAATGAAAACATTTTGCGGCTATTCTTGAAGTGTATGGTTTCaaattggaagcgc                                                                   >  2:389706/1‑240 (MQ=11)                                                                                                                                                                        cAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGtt                                                                >  2:217578/1‑251 (MQ=21)                                                                                                                                                                           gCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCACCCTGCCGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGCCAATCGCTGCGGTACTAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGATGTGTCTGGTTTTCAGATTAGCCCCCGTTCtg                                                             >  1:67926/1‑251 (MQ=11)                                                                                                                                                                                        cGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTACCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGtttt                                                 >  1:341747/1‑250 (MQ=18)                                                                                                                                                                                         ggCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTAc                                               >  1:178642/1‑251 (MQ=37)                                                                                                                                                                                          gCCGGTTTCGGGCGAATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGCCAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCAGGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATGAGCCCCCGTTCTGTTGTCAGGTTTTAcc                                              <  2:20194/251‑1 (MQ=255)                                                                                                                                                                                                 gcGGGCGCATATTGCCATCACGGCAGCCGGACGCCCGTTTTCACTTTACTTCCGGTTACGCCCCCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAAc                                       <  1:270597/250‑1 (MQ=255)                                                                                                                                                                                                       gcATATTGCCATCACGGCAGCCTGACGCCCGTTTTCTCTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGAGCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCgg                                 <  1:320522/251‑1 (MQ=255)                                                                                                                                                                                                         atatTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCgggg                               >  2:60596/1‑251 (MQ=37)                                                                                                                                                                                                                   cccGGCAGCCGGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATGCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTtctc                       <  2:435480/247‑1 (MQ=255)                                                                                                                                                                                                                      ggCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTcc                  >  1:98099/1‑251 (MQ=37)                                                                                                                                                                                                                                   ccGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGcc     <  1:447691/251‑1 (MQ=37)                                                                                                                                                                                                                                     gTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCACCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCTGGGGTTTTCTCTTTCCAGCAACCAATTccac   >  1:331688/1‑251 (MQ=255)                                                                                                                                                                                                                                       tttCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGGTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGccacc  <  1:58802/250‑1 (MQ=37)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    ATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACC  >  NC_000913/1269466‑1269945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑