Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,257,135	C→T	A226A (GCG→GCA)	ychF ←	redox‑responsive ATPase YchF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,257,135	0	C	T	100.0%	154.5 / NA	45	A226A (GCG→GCA)	ychF	redox‑responsive ATPase YchF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/20);  total (25/20)

GCCTGCGGCGCGGTTGCTCCAACCGGAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCCGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATACCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACG  >  NC_000913/1256902‑1257375                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 gcctgcGGCGCGGTTGCTCCAACCGGAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCTCGGATCACACGGTTCAGGCGCGGCGCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCCTTCGCTGCGATTTCACGCACCt                                                                                                                                                                                                                                  <  1:380687/250‑1 (MQ=255)  cctgcGGCGCGGTTGCTCCAACCGGAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTgg                                                                                                                                                                                                                                >  1:186525/1‑251 (MQ=255)     gcggcgCGGTTGCTCCAACCGGAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCa                                                                                                                                                                                                                             <  2:373290/251‑1 (MQ=255)       ggcgCGGTTGCTCCAACCGGAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCa                                                                                                                                                                                                                             >  2:112892/1‑249 (MQ=255)                        cGGAATGGTCAATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCa                                                                                                                                                                                                           >  2:553227/1‑250 (MQ=255)                         ggAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCaaa                                                                                                                                                                                                         <  1:133079/251‑1 (MQ=255)                          gAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCaaa                                                                                                                                                                                                         >  1:221658/1‑250 (MQ=255)                           aaTGGTCCATGCACGCTCTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAAc                                                                                                                                                                                                       <  1:209075/251‑1 (MQ=255)                                    tGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGATTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGATCTTCCAGCCCCAGCCCCTGCATAAACTCGTAACGTTCCTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACCGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTTGTCAAGATATGGTTTGTTTTCAAAACCTTCTTCGt                                                                                                                                                                                              >  1:252175/1‑251 (MQ=255)                                     gcacgcacTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGtt                                                                                                                                                                                             >  2:386856/1‑251 (MQ=255)                                                  tCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCCGCCCACGCTCCTGCATACACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCCTCGTTGACGTTGGCGATg                                                                                                                                                                                >  2:282724/1‑251 (MQ=255)                                                   cACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGGCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTGCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCTTTGACGTTGGCGATGt                                                                                                                                                                               <  2:295923/251‑1 (MQ=255)                                                      cccAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACa                                                                                                                                                                            <  1:160820/251‑1 (MQ=255)                                                          gCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACAttgt                                                                                                                                                                        >  1:500268/1‑251 (MQ=255)                                                               gAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGtt                                                                                                                                                                   <  1:466908/251‑1 (MQ=255)                                                                  gtaagtTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTc                                                                                                                                                                 >  1:469642/1‑250 (MQ=255)                                                                      gtTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGt                                                                                                                                                            >  2:125193/1‑251 (MQ=255)                                                                         tGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTc                                                                                                                                                           >  2:514644/1‑249 (MQ=255)                                                                         tGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCa                                                                                                                                                          <  1:65166/250‑1 (MQ=255)                                                                                 ccgcagTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCTCGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTc                                                                                                                                                  >  2:528466/3‑250 (MQ=255)                                                                                        ttATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAAc                                                                                                                                           <  2:186525/250‑1 (MQ=255)                                                                                                   aCGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCtt                                                                                                                               <  1:514644/251‑1 (MQ=255)                                                                                                     ggATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCtttt                                                                                                                             >  2:485168/1‑250 (MQ=255)                                                                                                       aTCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTtctc                                                                                                                           <  2:248063/251‑1 (MQ=255)                                                                                                       aTCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGACAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTATTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTtctc                                                                                                                           >  1:338381/1‑251 (MQ=255)                                                                                                          acacGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTc                                                                                                                        >  2:211852/1‑251 (MQ=255)                                                                                                                      cccGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATc                                                                                                            <  1:168235/251‑1 (MQ=255)                                                                                                                          gCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAg                                                                                                                                                               >  2:478662/1‑196 (MQ=255)                                                                                                                          gCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAg                                                                                                                                                               <  1:478662/196‑1 (MQ=255)                                                                                                                                   gCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCgcagc                                                                                               <  1:544912/251‑1 (MQ=255)                                                                                                                                         gCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATACCt                                                                                         <  1:112892/251‑1 (MQ=255)                                                                                                                                         gCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATACCt                                                                                         >  2:401327/1‑251 (MQ=255)                                                                                                                                           tCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACCTTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATACCTGc                                                                                       >  2:27308/1‑251 (MQ=255)                                                                                                                                            ccTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATACCTGc                                                                                       >  1:62853/1‑250 (MQ=255)                                                                                                                                                            aCGTTCTTCGTCGTCCAGTTCGGCAAGGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTGCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATACCTGCATTTTCCAACTGGggca                                                                      <  2:469642/251‑1 (MQ=255)                                                                                                                                                                                    aaTGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATACCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCcagct                                              >  1:475962/1‑251 (MQ=255)                                                                                                                                                                                       gTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATACCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCcagctca                                            <  2:15529/250‑1 (MQ=255)                                                                                                                                                                                            cTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATACCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTc                                      >  1:409903/1‑251 (MQ=255)                                                                                                                                                                                                   agcagcACAAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATACCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCtt                               >  1:409567/1‑251 (MQ=255)                                                                                                                                                                                                        cacaAACCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATACCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCg                          <  1:7466/251‑1 (MQ=255)                                                                                                                                                                                                           taaCCGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATACCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGcc                        >  2:148405/2‑250 (MQ=255)                                                                                                                                                                                                               cGGAACCACAACAGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATACCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCtt                   >  1:488434/1‑251 (MQ=255)                                                                                                                                                                                                                ggAACCACAACATAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGAGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATACCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCttt                  <  2:261818/251‑1 (MQ=255)                                                                                                                                                                                                                       caacaGAACCTTCTTTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATACCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTttc           >  1:203461/1‑251 (MQ=255)                                                                                                                                                                                                                                  tcttTCGCTGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGGTGGCGAGGTACATTGTTGGTTTCAGCGGCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATACCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTAcg  <  1:528466/249‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 GCCTGCGGCGCGGTTGCTCCAACCGGAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCAGCCCAAGCTCCTGCATAAACTCGTCACGTTCTTCGTCGTCCAGTTCGGCAATGTCTGCTTCAACAGCAGCACAAACCGGAACCACAACAGAACCTTCTTTCGCCGCGATTTCACGCACCTGGTCAAGATATGGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATACCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACG  >  NC_000913/1256902‑1257375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑