Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,235,755	C→T	S496N (AGC→AAC)	ycgB ←	PF04293 family protein YcgB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,235,755	0	C	T	100.0%	86.4 / NA	31	S496N (AGC→AAC)	ycgB	PF04293 family protein YcgB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/18);  total (13/18)

CCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTT‑GTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTTATATTGCGACGATAACCGGTTACGGATCTCCCGATAACCTTCTTCA  >  NC_000913/1235516‑1235988                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                          ccACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTT‑GTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCg                                                                                                                                                                                                                                >  2:4124/1‑251 (MQ=255)      aaCTGACGGCGTTGTGGAGTGAAGGCGCGCCCGACCAGGTGTACGAAACAGTGAGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGACATTCAGGGGCGATACTCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTT‑GTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGTTATTGACGTCTTCGttttt                                                                                                                                                                                                                           >  2:549827/1‑250 (MQ=255)                 ttGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTT‑GTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGc                                                                                                                                                                                                                 >  1:440329/1‑250 (MQ=255)                      cAGTGAGGGCGCGCACGATCAGGTGTCCGAAACAGTGCGTCGCTCTGGGCATGAGAGTGGCGAGATTTGGCAACGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTT‑GTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGCTGTTTCCGTCTTCGTTTTGCTGTTCGAGcatcac                                                                                                                                                                                                            >  2:355941/1‑250 (MQ=255)                                      gATCAGGTGTACGAAACAGGGCGTCGCTATGTGCATGAGAGTGGCGAGATTTGGCACCGGATGCATAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTT‑GTTGTTTTTACAGAGTTCCCATTCTTGGCGTGCAACGTTCCAGCAACTCGATGTTGCCGTCTGCGGTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGc                                                                                                                                                                                           <  2:34747/251‑1 (MQ=255)                                       aTCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTT‑GTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCg                                                                                                                                                                                          >  1:427412/1‑251 (MQ=255)                                                       aGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTT‑GTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACCTCAAATCCCCACAGGCGATGCAAATGCTTCAgg                                                                                                                                                                          >  2:482549/1‑251 (MQ=255)                                                                    gggCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTT‑GTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGAAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGAAGCACATGCTTCAGGACTTCTTTGCGcc                                                                                                                                                             >  1:392012/1‑251 (MQ=255)                                                                                ggCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTGTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTT‑GTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCTAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGc                                                                                                                                                  <  1:227310/250‑1 (MQ=255)                                                                                  cGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCGAACCCCTCAAACGAGGGGTTTTTG‑GTTGTTTTTACAGATTGCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAACCCCCACAGGCGATGCACATGCTGCAGGACGTCTTTGCGCCCCCGATCCAGCGGt                                                                                                                                               <  2:406725/251‑1 (MQ=255)                                                                                  cGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTT‑GTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGt                                                                                                                                               <  1:128419/251‑1 (MQ=255)                                                                                                                      tGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCACACCCCTCAAACGAGGGGTTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGTTCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCg                                                                                                             >  1:41788/1‑250 (MQ=255)                                                                                                                            aGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTT‑GTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTc                                                                                                      <  1:519289/250‑1 (MQ=255)                                                                                                                                                cccTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTT‑GTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCa                                                                                 <  2:183694/251‑1 (MQ=255)                                                                                                                                                cccTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTT‑GTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCa                                                                                 <  2:260939/251‑1 (MQ=255)                                                                                                                                                        tttAAAGAGCAAACCCCTCAAACGAGGGGTTTTTT‑GTTGTTTTTACAGATTTCCCATTCTTGGCGGGCACCGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGa                                                                          >  1:306187/1‑250 (MQ=255)                                                                                                                                                         ttAAAGAGCAAACCCCTCAAACGAGGGGTTTTTT‑GTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCGTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGGCGCCGCGCAAATcc                                                                                         <  1:88609/234‑1 (MQ=255)                                                                                                                                                         ttAAAGAGCAAACCCCTCAAACGAGGGGTTTTTT‑GTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTAGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATACCCACAGGCGCTGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGAGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATcc                                                                                         >  2:88609/1‑234 (MQ=255)                                                                                                                                                              gagCAAACCCCTCAAACGAGGGGTTTTTT‑GTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCg                                                                   <  1:164842/251‑1 (MQ=255)                                                                                                                                                               agCAAACGCCTCAAACGAGGGGTTTTGT‑GTTGTTTTTATAGATTTTCCATGCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCATCTTCGTTTTGCTGGTTGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGGGGAATATAACGCAGCGTCAGCGAACGGTCGATGCGCAAATCCACGTTCCAGATATGAATATTctg                                                                  <  2:331639/251‑3 (MQ=255)                                                                                                                                                               agCAAACCCCTCAAACGAGGGGTTTTTT‑GTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTTCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATTCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCgg                                                                  <  1:332813/251‑1 (MQ=255)                                                                                                                                                                    aCCCCTCAAACGAGGGGTTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGTTCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTcc                                                              <  2:41788/251‑1 (MQ=255)                                                                                                                                                                     ccccTCAAACGAGGGGTTTTTT‑GTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGCGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGTTCTGAATATTCGGCTcc                                                              >  1:49615/1‑249 (MQ=255)                                                                                                                                                                     ccccTCAAACGAGGGGTTTTTT‑GTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTTTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTcc                                                              >  1:436122/1‑249 (MQ=255)                                                                                                                                                                                        ttt‑GTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGAATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTTATATTg                                          <  1:219165/250‑1 (MQ=255)                                                                                                                                                                                                       aGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTTATATTGCGACGATAACCGGtt                           <  2:440329/251‑1 (MQ=255)                                                                                                                                                                                                                aTTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGGTATATTGCGACGATAACCGGTTACGGAtctc                  >  1:519225/1‑251 (MQ=255)                                                                                                                                                                                                                     tGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTTATATTGCGACGATAACCGGTTACGGATCTccc                <  1:262772/248‑1 (MQ=255)                                                                                                                                                                                                                     tGGCGGGCAACGTTCCAGCAACGCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTTATATTGCGACGATAACCGGTTACGGATCTCCCg               <  2:308139/249‑1 (MQ=255)                                                                                                                                                                                                                          ggCAACGTTCCAGCATCTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTTATATTGCGACGATAACCGGTTACGGATCTCCCGATAACct        <  2:578409/251‑1 (MQ=255)                                                                                                                                                                                                                                gtcccAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTTATATTGCGACGATAACCGGTTACGGATCTCCCGATAACCTTCTTCa  <  2:25715/248‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                          CCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTT‑GTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTTATATTGCGACGATAACCGGTTACGGATCTCCCGATAACCTTCTTCA  >  NC_000913/1235516‑1235988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑