Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,197,637	C→T	G201S (GGT→AGT)	ymfE ←	uncharacterized protein YmfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,197,637	0	C	T	100.0%	70.7 / NA	21	G201S (GGT→AGT)	ymfE	uncharacterized protein YmfE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/13);  total (8/13)

TTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATT  >  NC_000913/1197416‑1197883                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                       tttCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACaaaa                                                                                                                                                                                                                            <  1:83134/250‑1 (MQ=255)        aTGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATaa                                                                                                                                                                                                                    <  1:393303/251‑1 (MQ=255)             tCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGAc                                                                                                                                                                                                                >  2:156371/1‑250 (MQ=255)               tAATATACTCGCAAAGATCTCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATCGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGc                                                                                                                                                                                                             >  1:180638/1‑251 (MQ=255)                aaTATACTCTCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTTCCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGACAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACa                                                                                                                                                                                                               <  1:70301/248‑1 (MQ=255)                                     agagGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGaaaaa                                                                                                                                                                                       <  1:450158/251‑1 (MQ=255)                                       agGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAat                                                                                                                                                                                      >  2:148237/1‑250 (MQ=255)                                          gCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATcc                                                                                                                                                                                  >  1:58562/1‑251 (MQ=255)                                            aTCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCAt                                                                                                                                                                                <  2:396100/251‑1 (MQ=255)                                                                      tGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCCGTTCTGGGCTTAACTGTCAAAGAAAAGATAATACTACTAACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTACAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTagg                                                                                                                                                       >  1:333953/1‑250 (MQ=255)                                                                                 tAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTaa                                                                                                                                            <  1:413333/250‑1 (MQ=255)                                                                                       cGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAAGTAATGCAAtt                                                                                                                                     <  1:156371/251‑1 (MQ=255)                                                                                        gTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTa                                                                                                                                    <  1:417789/251‑1 (MQ=255)                                                                                          gCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATc                                                                                                                                  <  2:462395/251‑1 (MQ=255)                                                                                                  taGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCAGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTc                                                                                                                          >  2:392282/1‑251 (MQ=255)                                                                                                                               gTTATTTTAAGAGAAAAATTCTTTTATTTTATCATCCGGGATATTTAACACTTGACTTTCCGTTATGCGCTTAACTGTCAAATCAAAGATCATAATCCTCACTGCGAATGATAAACTTACATAACAGAATAAAGCCAGCCAAGAACATTCACCATGAAATATATACATTTATTTGCATAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCTAAAAATATCCAAAAATATTATACTTTAt                                                                                             >  2:214877/1‑251 (MQ=255)                                                                                                                                                     tttattttatCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTAGTAGGAAGGAAAGTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTAGTCTTTATTCCAGAAGAGTTCAATATAAt                                                                        <  2:242360/250‑1 (MQ=255)                                                                                                                                                                             acGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGAGAATACTACTCACTGAGAATGATAAAATTCCAAAAAAGAATAAAGCCAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACtt                                                <  1:18914/249‑1 (MQ=255)                                                                                                                                                                                       tttCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAatat                                     >  1:11721/1‑251 (MQ=255)                                                                                                                                                                                                  ggCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCAt                          <  1:392282/251‑1 (MQ=255)                                                                                                                                                                                                                          ataCTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCtttatt  <  1:148237/251‑1 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                       TTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATT  >  NC_000913/1197416‑1197883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑