Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,175,517	A→G	T31A (ACC→GCC)	lolC →	lipoprotein release complex ‑ inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,175,517	0	A	G	96.4%	195.7 / ‑4.2	55	T31A (ACC→GCC)	lolC	lipoprotein release complex ‑ inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (37/16);  total (39/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

AGGCGGCAAGATTGATGATAAAACATGGCAATTTAGCCGATTGATTTACGGGGGCTTTTCAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGG  >  NC_000913/1175272‑1175763                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       aGGCGGCAAGATTGTTGCTAAAACATGGCAATTTAGCCGATTGATTTACGGGGGCTTTTCAGATTAGACCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGTTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATc                                                                                                                                                                                                                                                   >  2:519562/1‑251 (MQ=255)  ggcggcAAGATTGATGATAAAACATGGCAATTTAGCCGATTGATTTACGGAGGCTTTTCAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCg                                                                                                                                                                                                                                                  <  2:201657/251‑1 (MQ=255)    cggcAAGATTGATGATAAAACATGGCAATTTAGCCGATTGATTTACGGGGGCTTTTCAGATTAGCCCTGCCGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGc                                                                                                                                                                                                                                                >  1:553864/1‑251 (MQ=255)     ggcAAGATTGATGATAAAACATGGCAATTTAGCCGATTGATTTACGGGGGCTTTTAAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGc                                                                                                                                                                                                                                                >  2:192005/1‑250 (MQ=255)      gcAAGATTGATGATAAAACATGGCAATTTAGCCGATTGATTTACGGGGGCTTTTCAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCAt                                                                                                                                                                                                                                              <  1:28319/251‑1 (MQ=255)             tgatgaTAAAACATGGCAATTTAGCCGATTGATTTACGGGGGCTTTTCAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCt                                                                                                                                                                                                                                        <  2:163162/250‑1 (MQ=255)              gattatAAAACATGGCAATTTAGCCGATTGATTTACGGGGGCTTTTCAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCg                                                                                                                                                                                                                                      >  2:140054/5‑251 (MQ=255)              gatgatAAAAGATGGCAATATAGCAGATCGATTTACGGGGGCTTTTCAGATTAGCCCTGACGATCAGTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCg                                                                                                                                                                                                                                      >  2:356095/1‑251 (MQ=255)               atgatAAAACATGGCCATTTAGCCGATTGATTTACGGGGGCTTTTCAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCgg                                                                                                                                                                                                                                     >  2:372040/1‑251 (MQ=255)                     aaaCATGGCAATTTAGCCGATTGATTTACGGGGGCTTTTCAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGAt                                                                                                                                                                                                                               >  1:430289/1‑251 (MQ=255)                       aCATGGCAGTTTAGCCGATTGATTTACGGGGGCTTTTCAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACACATCTTGCTTTCGCTTATATACTCGTGGCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATgg                                                                                                                                                                                                                             >  1:529378/1‑251 (MQ=255)                              aaTTTAGCCGATTGAGTTACGGGGGCTTTTCAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGt                                                                                                                                                                                                                      <  1:44476/251‑1 (MQ=255)                                     ccGATTGATTTACGGGGGCTTTTCAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTCCCCTCGGGGTGATGGCGCTGGTCACAGt                                                                                                                                                                                                                >  2:184252/1‑250 (MQ=255)                                               tACGGGGGCTTTTCAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGtg                                                                                                                                                                                                      >  1:295146/1‑250 (MQ=255)                                                        ttttCAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGCGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCt                                                                                                                                                                                            >  1:146077/1‑251 (MQ=255)                                                            cAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTg                                                                                                                                                                                         >  1:176423/1‑250 (MQ=255)                                                                  aGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCACCCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGCATTGTCAGTGATGAACGGCTTTGAGcgcg                                                                                                                                                                                   >  1:545298/1‑250 (MQ=255)                                                                  aGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGcgcg                                                                                                                                                                                   <  1:163387/250‑1 (MQ=255)                                                                        gACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTg                                                                                                                                                                              <  1:365743/249‑1 (MQ=255)                                                                            aTCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAaac                                                                                                                                                                        >  1:352544/1‑251 (MQ=255)                                                                                        tCAGACGTTTACCAATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGCCGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGc                                                                                                                                                            >  2:47990/1‑251 (MQ=255)                                                                                        tCAGACGTTTACCAATCATACTTTCGCTTATATACTCGTGTATTTGCTACAGCAACCTTTCGTTTTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGATACAGGCGAGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTCCCATAGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGATTTGAGCGCGCGCTGCAAaacacaatcctgggc                                                                                                                                                            >  2:415751/1‑240 (MQ=255)                                                                                              gTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTgctg                                                                                                                                                      >  2:119774/1‑248 (MQ=255)                                                                                                 tACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGccc                                                                                                                                                   >  2:13301/1‑250 (MQ=255)                                                                                                  aCCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCcac                                                                                                                                                  >  1:327302/1‑251 (MQ=255)                                                                                                          tGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAAtt                                                                                                                                          >  1:157202/1‑251 (MQ=255)                                                                                                                  tttATATACTCGTGACTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGGGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCATGGCCTGATGCCACAGGCAATTCTCtcttc                                                                                                                                  >  2:327321/2‑251 (MQ=255)                                                                                                                     atataCTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTTCCAACCTGTCGCTCTATTTATTGGCCTGCGTTACTTGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCGTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTGTTGTCAGGGATTAACGGCTGTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTg                                                                                                                                <  1:92001/250‑1 (MQ=255)                                                                                                                        taCTCGTGTCTTTGCTACAGAAACCAGCCGGATTTCATGTACCCACCTTTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGGGCTGGCGCTGGTCACAGTATTGTCAGGGATGAACGGCTTTGAGCGCGAGCTGCAAACCAACCTCCTTGGCCTGATGCCACAGGCCATTCTCTCTTCTGAGc                                                                                                                             >  2:189608/1‑250 (MQ=255)                                                                                                                         aCTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCa                                                                                                                            <  2:352544/250‑1 (MQ=255)                                                                                                                          cTCGTGTATTTGCTACAGGAACAAGAAGGACTTCATATAGCAACCTGCCGCACTCTTTATTGCCCTACGTTACATGCGTGGGCGTGCAGCGGATAGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGTGCGAGCTCCCAACCAACATCCTTGGCCTGATGCGACAGGCACTTCTCCTTTCTGAACAtc                                                                                                                          >  2:371319/1‑250 (MQ=255)                                                                                                                             gtgtCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTAGGCGTACAGCGGATCGCTTCGGTCGTTTCGTATCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGTTGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAAAATCCTTGGCCTGATGCCACAGGAAATTCTATATTCTGAGCATGGct                                                                                                                       >  2:30887/1‑251 (MQ=255)                                                                                                                                                cAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAAcc                                                                                                    >  2:254282/1‑250 (MQ=255)                                                                                                                                                     ggATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTccc                                                                                                 >  1:561406/1‑249 (MQ=255)                                                                                                                                                                       tGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTgg                                                                             >  1:397587/1‑251 (MQ=255)                                                                                                                                                                             tctATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCg                                                                       >  1:399161/1‑251 (MQ=255)                                                                                                                                                                                 tttattGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTa                                                                    >  1:187780/1‑250 (MQ=255)                                                                                                                                                                                  ttattGGCCTGCGTTACAGGCGTGGGCGTGCAGCGGATCGCTTCGTTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTTTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCACGTCGGAGCATGGCTCTCTTAACCCTCAGCAACTCCCAGAAACGGCATTCAAACTCGACGGCGTTaa                                                                   <  2:234980/250‑1 (MQ=255)                                                                                                                                                                                    attGGCCTGCGGTGCATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTGCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATc                                                                 <  2:167007/250‑1 (MQ=255)                                                                                                                                                                                         ccTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCg                                                           <  2:430289/251‑1 (MQ=255)                                                                                                                                                                                           tGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGc                                                          >  2:254744/1‑250 (MQ=255)                                                                                                                                                                                                 aCATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAATCTGGACGGCGTTAATCGCGTCGCACCTAtt                                                   <  1:162104/251‑1 (MQ=255)                                                                                                                                                                                                   aTGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATtac                                                 >  2:85146/1‑251 (MQ=255)                                                                                                                                                                                                   aTGCGCGGGCGTGCAGCAGATCGCTTCGGTCGTATAGTCTCATGGCTTTCTGCCACAGGCATTACCCTGGGGGTGATGGCGATGGTCACAGTATTGTCAGTGATGCAAGGCTTTGAGCGCGCGCTGCAAAACAACATCATTGGAATGAGGCCAAAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACAGGCCGGAGTTCATCGAGTCGCACCTATtac                                                 >  2:46579/1‑251 (MQ=255)                                                                                                                                                                                                      cGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATtactac                                              >  1:128790/1‑251 (MQ=255)                                                                                                                                                                                                       gTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCgcagc                                                                                                        >  2:118797/1‑192 (MQ=255)                                                                                                                                                                                                       gTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCgcagc                                                                                                        <  1:118797/192‑1 (MQ=255)                                                                                                                                                                                                         gggCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCgg                                           <  1:267786/251‑1 (MQ=255)                                                                                                                                                                                                            cGTGCAGCGGATCACTCCGGTCGTTTCGTCCCCTGGCTTTATACCATCAGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTg                                         >  2:107280/1‑250 (MQ=255)                                                                                                                                                                                                             gTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGAt                                       <  1:120135/251‑1 (MQ=255)                                                                                                                                                                                                              tGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGAtg                                      >  1:394600/1‑251 (MQ=255)                                                                                                                                                                                                                 aGCGGACCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCCTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTgg                                   <  1:303570/251‑1 (MQ=255)                                                                                                                                                                                                                                    ttCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAgcgcgcgc                  >  2:154688/1‑249 (MQ=255)                                                                                                                                                                                                                                         ctcCTGGCTTTCTGCCATCGGCATTACACTCGGGGTGATGGCGCTGGTCACCGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTCCTACCGGCGATGTGGTACTGCAAAGCGCGCGCAGCGTgg           >  2:358977/1‑251 (MQ=255)                                                                                                                                                                                                                                                  ttCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCgggg  >  1:381017/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       AGGCGGCAAGATTGATGATAAAACATGGCAATTTAGCCGATTGATTTACGGGGGCTTTTCAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGG  >  NC_000913/1175272‑1175763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑