| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,174,883 | C→T | A95T (GCG→ACG) | ycfT ← | inner membrane protein YcfT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,174,883 | 0 | C | T | 90.9% | 89.7 / NA | 34 | A95T (GCG→ACG) | ycfT | inner membrane protein YcfT |
| Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (8/22); minor base G (3/0); total (12/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.02e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATCACCACCAGACAAATA > NC_000913/1174637‑1175121 | ccACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGTCAGc < 2:529468/251‑1 (MQ=255) acaaacaaGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACCGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGtt < 2:176531/251‑1 (MQ=255) caaGGCGAATAGTGGCAGCGCCAGACTGCTAAAAATTTTACATACCACGAAATAGACAAATAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGa < 1:225410/251‑1 (MQ=255) aaGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCCGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGGCAGCACCCAGAAGATGTTcc > 1:382904/1‑247 (MQ=255) ggCGAATAGTGGCAGCGCCAGACGGCTAAAGATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGGGGAAACGGCATAAGCGGCATTGGAAGCATTGCTTAAATCGCGCTCAGGTGCCCGCCCCTGGGTCAGCGCACTTAACGCCCACCACTGCCCCCCGCCCCCAAGCGGCAGCCCCCAGAAGATGTTCCCGATcc > 1:130516/1‑251 (MQ=255) gggCAGCGCCAGACGGCTAAAAATTT‑ACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCgag < 2:572182/250‑1 (MQ=255) cAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCCTACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCTCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAATCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGAc < 1:420764/251‑1 (MQ=255) cataCGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCCCCGGTGGAATCGGCATAAGCGGCCTTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCCCTGCACCACGCCCCCAAGCGTCAGCACCCAGAAGATGTTCCCGATCCGTTTATCGAGACCATTTc > 2:144667/4‑251 (MQ=255) gACGGCTAAAAATTTTACATACCACGACATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATAATCCCGTGCAGGAACTCACCGGAGGAATCGGCATAAGAGGCATTGGAGGCATTGCTTAAAACGCGCGCAGGTGCCAGCCACTGGTTCAGCGCACTTAAAGCCAGCCAATGCACCACGCCCCAAAGCGCCAGCACCCCGAAGGTATTCCCGAACCGCTTATCGAGACAAttttcc > 1:307009/1‑248 (MQ=255) cTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACACGCTGGTGCTGGCGGGGATTATACCCTGCAGGAACCCACCCGGGGAATCGGCCTAAGAGGCATTGGAGGCATTTCTTAAATCGCGCTAAGGGGCCACCCACTGGTTCCGCGCAATTAACACCAGCCCCTGCACCACGCCCCCAAACGTCAGCACCCAGAAAGTGTTACAGATCCGTTTGTCGAAACAATTTcccccc > 2:308784/1‑247 (MQ=255) ttACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCTGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGt < 1:206076/250‑1 (MQ=255) aaaTAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAgcggcg < 2:569980/251‑1 (MQ=255) aCAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCa > 1:142174/1‑250 (MQ=255) aattaccccATACAGATACCACAGGCTGGTGCTGGCGTTGATCATCCCGTGCAGGAACTCACCGGTTGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGTTTCCAGCCACTGGTTCAGCGCACTTAACTCCAGCCACTGCACCACGCCCCAAATCGTCAGCACCCAGAAGATGTTCCAGATCCTTTTATCGAGACAATTTCCCCACGGCACGCTGTCTATATAGCTGCGTATCAAAt < 2:291604/243‑1 (MQ=255) aaTTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAAt < 2:4818/251‑1 (MQ=255) caAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGGGGGATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCCCGCCCCAAAGCCGCAGCACCCCGAAGATGTTCCCGATCCGTTTATCGAGACAATTTCCCCCCGGGACGCCGTCGATATAGCGGGGAATCAAATAGCCGGGTATAAAGgaaaaa > 2:321433/1‑250 (MQ=255) aaGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGGTATAAAGAAAAAAAc > 1:23586/1‑251 (MQ=255) gggTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAg < 2:146372/250‑1 (MQ=255) gATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAggggg < 1:504002/251‑1 (MQ=255) caggaatccACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGAt < 2:130516/243‑1 (MQ=255) aGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAgc > 1:220592/1‑187 (MQ=255) aGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAgc < 2:220592/187‑1 (MQ=255) gAACTCTCCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGa < 2:138218/251‑1 (MQ=255) cGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCAttt < 1:562058/250‑1 (MQ=255) ggCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGAAAGCTGTCGATATAGCGGCGACTCAAATAGCCGGATATAAAGAAAAAAACAGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGc > 1:314067/1‑251 (MQ=255) cgcgCTCAGGTGCCAGCCACTGGTTCAACGCACGTAACGCCAGCCACTGCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTg < 1:308784/251‑1 (MQ=255) gcgcTCAGGTGCCAGCCACTGGTTCAGCGCACTTATCGCCAGCCACTGCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTgg < 2:208269/251‑1 (MQ=255) gcTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGGCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGTGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTc < 2:586040/251‑1 (MQ=255) ggTTAAGCGCACTTAACGCCAGCCAATGCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAAGCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAgg < 2:118238/249‑1 (MQ=255) cgcACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCg < 1:250013/251‑1 (MQ=255) aaCGCCAGCCACTGCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAACAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGttat > 2:68604/1‑247 (MQ=255) gCACCACGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATCACCACCAg < 1:68604/250‑1 (MQ=255) ccacGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATCACCACCAGACa > 2:522868/1‑250 (MQ=255) cGCCCCAAAGCGTCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCAGTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATCACCACCATTCAAATa < 2:235594/251‑1 (MQ=255) | CCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATCACCACCAGACAAATA > NC_000913/1174637‑1175121 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |