Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,147,196	C→T	P135S (CCG→TCG)	yceD →	DUF177 domain‑containing protein YceD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,147,196	0	C	T	100.0%	217.3 / NA	65	P135S (CCG→TCG)	yceD	DUF177 domain‑containing protein YceD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/29);  total (36/29)

AACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTCCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGT  >  NC_000913/1146956‑1147440                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     aaCCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATg                                                                                                                                                                                                                                             >  1:420945/1‑250 (MQ=255)    cAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTc                                                                                                                                                                                                                                         >  1:43213/1‑251 (MQ=255)        gTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGa                                                                                                                                                                                                                                      >  1:78229/1‑250 (MQ=255)               aGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACtgtg                                                                                                                                                                                                                              >  2:170339/1‑251 (MQ=255)                  gtTAAACGGCGATGCGCAGGTGACGGTAAAGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGCCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGGTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAACTCATCCTCGCCTTGCCGGTAGTTTAGGTGCATGATTATGCACACtgaga                                                                                                                                                                                                                             >  2:343585/1‑246 (MQ=255)                      aaCGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTc                                                                                                                                                                                                                       >  1:285074/1‑251 (MQ=255)                       aCGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTcc                                                                                                                                                                                                                      <  2:319782/251‑1 (MQ=255)                         ggCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCg                                                                                                                                                                                                                     >  1:408211/1‑250 (MQ=255)                             aTGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCg                                                                                                                                                                                                                <  1:414966/251‑1 (MQ=255)                               gCGAAGGTGACGGTAACGCTCGATTGTCAGCTTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAATCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTTCTTGCAATGGTTGAAGATGAAATCATGCTCGCCTTGCCGGTAGGTTCGGTGCATGATTCGGAACACTGTGAAGTGTCCGAAGCGGa                                                                                                                                                                                                              <  2:60984/251‑1 (MQ=255)                                cGAAGGAGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGGGCATGATTCTGAACACTGTGACGTGTCCGAAGCGGAc                                                                                                                                                                                                             >  1:579945/1‑251 (MQ=255)                                    ggTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATgg                                                                                                                                                                                                         <  1:170339/251‑1 (MQ=255)                                        aCGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCtt                                                                                                                                                                                                     <  1:551751/251‑1 (MQ=255)                                               cGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGaa                                                                                                                                                                                              <  2:178889/251‑1 (MQ=255)                                                  tCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTg                                                                                                                                                                                           >  1:462285/1‑251 (MQ=255)                                                        gtCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGACTTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTATTTGGTGACCTGCCTga                                                                                                                                                                                      >  1:376896/1‑250 (MQ=255)                                                             cGTTGCGGGAAGCCGATTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAAACGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAgc                                                                                                                                                                                >  1:319758/1‑251 (MQ=255)                                                              gTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAgcg                                                                                                                                                                               <  1:15398/251‑1 (MQ=255)                                                                   gggAAGCCGTTTACTCATCCGGTCTACACACCGTCTTGTTTTAGTCCTGTGCGTTCAGCCGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGCTTCTGAACACTGTGAAGTGTCCGACGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCaaa                                                                                                                                                                           >  2:82772/1‑250 (MQ=255)                                                                    ggAAGCCGTTTACTCATCAGGGCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCGTGCAATGTGTGAAGATGAAATCATCCTCGCCTTGCCTGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAATAAGCGCAAAAg                                                                                                                                                                         <  2:566268/251‑1 (MQ=255)                                                                       aGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCa                                                                                                                                                                      <  1:475502/251‑1 (MQ=255)                                                                         ccGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCaa                                                                                                                                                                     >  1:423199/1‑250 (MQ=255)                                                                           gTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAAcc                                                                                                                                                                  >  1:320109/1‑251 (MQ=255)                                                                                      aGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTc                                                                                                                                                                                                                       <  1:268855/187‑1 (MQ=255)                                                                                      aGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTc                                                                                                                                                                                                                       >  2:268855/1‑187 (MQ=255)                                                                                        gTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTAtt                                                                                                                                                     <  1:216677/251‑1 (MQ=255)                                                                                               caACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGc                                                                                                                                              <  1:103304/251‑1 (MQ=255)                                                                                               caACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGATAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGGGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGc                                                                                                                                              <  1:296671/251‑1 (MQ=255)                                                                                                         ttttAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCAGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTCGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACACATTTGCCGTATTAGCCAGCTTAAAGCgt                                                                                                                                     >  2:489366/1‑250 (MQ=255)                                                                                                             aGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCgtaagt                                                                                                                                 <  2:261045/250‑1 (MQ=255)                                                                                                               tCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAAt                                                                                                                              >  1:204184/1‑251 (MQ=255)                                                                                                                ccTGTGCGTTGAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCTTAAGTTAtt                                                                                                                             <  2:330640/251‑1 (MQ=255)                                                                                                                  tgtgCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTgg                                                                                                                           >  1:313490/1‑251 (MQ=255)                                                                                                                   gtgCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCCATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTACAGCGTAAGTAATTGGt                                                                                                                          >  2:268957/1‑251 (MQ=255)                                                                                                                      cggtCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGAGTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGca                                                                                                                       <  2:423199/248‑2 (MQ=255)                                                                                                                         tCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTccc                                                                                                                    >  1:350244/1‑251 (MQ=255)                                                                                                                              cGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTg                                                                                                               <  2:285074/251‑1 (MQ=255)                                                                                                                               gAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTgg                                                                                                              <  1:545468/251‑1 (MQ=255)                                                                                                                                  cAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATc                                                                                                           <  2:19866/251‑1 (MQ=255)                                                                                                                                      cTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGATGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTACGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCAAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCgggg                                                                                                       >  2:543013/1‑251 (MQ=255)                                                                                                                                           gCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATaaa                                                                                                  <  2:31925/251‑1 (MQ=255)                                                                                                                                             aCTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAAcc                                                                                                <  1:403556/251‑1 (MQ=255)                                                                                                                                                   ggAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGAACTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGAGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATACACCGTAAtt                                                                                          >  1:544819/1‑251 (MQ=255)                                                                                                                                                    gAAGCGTATGAACCGATTGAGGTTAACGACTTCGGTGAAATCGCTCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACAATGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAACAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTg                                                                                         >  2:586708/1‑251 (MQ=255)                                                                                                                                                          tATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATACTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTgcggtgt                                                                                   >  2:480357/1‑245 (MQ=255)                                                                                                                                                               aCCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGt                                                                              <  2:320109/251‑1 (MQ=255)                                                                                                                                                                      gAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATgg                                                                        <  2:43213/250‑1 (MQ=255)                                                                                                                                                                      gAGGTCAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCACGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGCGAAGTGACCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCCGCTTAAAGAGTAAGTAATTGGTGCTCCCCGTTGGCTCGGGGATAAACCGTAATTgagtagaaagtgccatgg                                                                        >  2:282006/1‑235 (MQ=255)                                                                                                                                                                         gTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACGGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGt                                                                    <  1:586708/251‑1 (MQ=255)                                                                                                                                                                                aaTTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGCAATCATCCTCGCCTTGCCGGGAGTTTCGGGGCATGATTCTGAACTCTGTGAAGTGGCCGAGGCGGGCAGGGGCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTGAAGGCGTAAGTAATAGGTGCTCCCCGTTGGATCGGGGGTAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTacaac                                                               >  2:14869/1‑249 (MQ=255)                                                                                                                                                                                aaTTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTaaa                                                                                                                                         >  2:581746/1‑175 (MQ=255)                                                                                                                                                                                aaTTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTaaa                                                                                                                                         <  1:581746/175‑1 (MQ=255)                                                                                                                                                                                      gTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCTTGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAATCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACTGTAATTGAGGATTAAGGTCCATGGCCGTACAACAGAATaaa                                                       <  1:201264/251‑1 (MQ=255)                                                                                                                                                                                        gAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTCGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCAGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAaacc                                                     >  2:34906/1‑251 (MQ=255)                                                                                                                                                                                                 cTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTc                                            >  2:437924/1‑251 (MQ=255)                                                                                                                                                                                                   gCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAAccc                                                <  2:408211/245‑1 (MQ=255)                                                                                                                                                                                                   gCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCa                                          <  1:422179/251‑1 (MQ=255)                                                                                                                                                                                                                gAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGcg                             >  2:537149/1‑251 (MQ=255)                                                                                                                                                                                                                              ccTCGCCTTGCCGGTAGTTTCGGTTCATTATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAATTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAGTAGAATAAACCAGCCCGTTCCAAACGTGGCATGCGTCGTTCCCATGAc                <  2:414849/250‑1 (MQ=255)                                                                                                                                                                                                                              ccTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATACACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCACACGTGGCATGCGTCGTTCCCATGAcg               >  1:112652/1‑251 (MQ=255)                                                                                                                                                                                                                                  gCCTTGCCGGGAGTTTCGGTGCATGATTCTGAACACTGTGAAGGGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAACGCCAAACCCATTTGCCGAATTAGCCAGCTTAAAGCGTAAGTAATTGGCGCTCCCCGTTGGATCGGGGATAAACCGTGATTGAGGAGTAAGGCCCATGGCCGTACAACAGACTAAACCAACACGTTCCAAACGAGGCATGCGTCGTTCCCATGACGCGCt           >  1:308326/1‑251 (MQ=255)                                                                                                                                                                                                                                      tGCCGGTCGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCCAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGAcc       >  2:431380/1‑251 (MQ=255)                                                                                                                                                                                                                                      tGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGAcc       >  2:36559/1‑251 (MQ=255)                                                                                                                                                                                                                                         cGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGc     >  1:324711/1‑250 (MQ=255)                                                                                                                                                                                                                                           gTAGTTTCGGAGCATGATTCTAAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTAAAACAGAATAAACCAACCAGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGccgt  >  2:272414/1‑248 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     AACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTCCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGT  >  NC_000913/1146956‑1147440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑