| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,138,130 | C→T | P254S (CCG→TCG) | flgJ → | putative peptidoglycan hydrolase FlgJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,138,130 | 0 | C | T | 96.5% | 142.9 / NA | 59 | P254S (CCG→TCG) | flgJ | putative peptidoglycan hydrolase FlgJ |
| Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (27/29); minor base G (0/2); total (27/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACC‑ACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAG > NC_000913/1137885‑1138369 | atTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACC‑ACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCgcgc > 1:461778/1‑251 (MQ=255)atTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACC‑ACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCgcgc > 1:328580/1‑251 (MQ=255) tCCTCGCGCAGGCTGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACC‑ACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTCTCTGGTAGCCTTGTCGGATTACGTTGGGATGTTAACGC‑GTAACTCGCGCTCcgcc > 1:2077/1‑251 (MQ=255) cccTCGCTCAGGCGGCCCTGGAATTTGGTGGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACC‑ACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTAcgcc < 2:511495/250‑1 (MQ=255) ccccagccggcACGGGAATTTGGTGGGGGGAAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAACCACC‑ACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCTCGCTACGCCGCCGt < 2:563106/244‑1 (MQ=255) cggcACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACC‑ACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGAcc > 1:536369/1‑251 (MQ=255) ggattttgtttGGGGGCAAGGGCAAACCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCCCC‑ACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTAAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGa < 2:177084/242‑1 (MQ=255) ttGGGGGCAACGGCAACTCCGCCGCGAAAACGGCGAGACGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACC‑ACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTAAAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGAGCTACGCCGCCGTGACGCCCGCCGCGCATGCGGAAc > 2:396538/1‑251 (MQ=255) ggCACCGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGGGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAAGCACC‑ACGACGGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTCCAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAgggg < 1:354493/251‑1 (MQ=255) gacaccGGCAAACCCCCCGCAAAAACGGCGAGCCGAGCTATACCCTGTTTGGTGTCAAAGCCTCTGGCAACGGGAAAGGGCCAGTTACGGAAATCACC‑ACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAGGCGAAGTGGCGCGTCTACAGCTCGTGTCTGGAAGCCTTGTCGGATTACGTTGGGCTTTTAACGC‑GTAACTCGCGCTACGCCGCTGTGACGGCCGCCGCTAGTGCGGAAGAgggg < 2:87233/246‑1 (MQ=255) cagcgtggagtCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACC‑ACGACTGAATATGAAAACAGCGTAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTCCAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGCGTGCGGCACAGGTGgc > 2:182859/11‑251 (MQ=255) cgcgAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACC‑ACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAgg > 1:358054/1‑250 (MQ=255) cgcgAAAACGGCGAGCCGAGCTATAACCTGTGTGGTTTCAAAGCCTCTGGAAACTGGAAAGGGCCAGTTACGGAAATCTCC‑ACGACTGAATATGAAACCGGCGAAGCGAAGAAAGGAAAAGCGAAGTTCCGCGTCTACAGCTCGTGTCTTGAAGCCTTGTCGGATTACGTTGGGCGGTTAACTC‑GTAACCCGCGCTACTGCGCCGGTACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGa < 1:43076/251‑1 (MQ=255) gcgAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACC‑ACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAGCTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGCACAGGGGGCGCAGGCCCTACAGGAc > 2:236343/1‑251 (MQ=255) aaaCGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACC‑ACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGCGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCgg > 2:520783/1‑251 (MQ=255) ggtgacaagctcctgggCAACTGGAAAGGCACTGTT‑CTGTAATGACCCACGCCTGAAGTTGAAAATGGAGAATCGATGAAATTAAAAGCGAATTTTCGCGTTTCCAGCTCGTATCTGTAGGCCTTGCCGTATTACGTTGGGCT‑TTAACCCCGAAACTCGCGCTACGCCGCCGGGACGACCGCCGCGAGTGCGGAACAGGGGGCTCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCGCTCTATGCCCg < 2:17429/236‑1 (MQ=255) gtCAAAGCCTCTGGCAGCGGGAAAGGGCCATGTACTGAAATCACC‑CCGTCTGAATGTGAAAAGGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCTTATCTGGAAGCCTTGTCGGATTACGTTGGCCTGTTAACGC‑GTACCTGGCGCTGCGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGGTATGC‑CACCGAGCCTCACTATGCCCGCaa < 2:250386/251‑1 (MQ=255) cAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACC‑ACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAAc > 2:436635/1‑251 (MQ=255) ccTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACC‑ACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCCCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCAcc < 1:345648/251‑1 (MQ=255) ctGGCAACTGGAAAGGGCCAGTTACTGAAATCACC‑ACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAAc > 2:460624/1‑251 (MQ=255) aaCTGGAAAGGGCCAGTTACTGAAATCACC‑ACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACTTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGGGCGGAACAGGGGGCGCAGGCACTACAGGGCGCGGGCTCTGC‑GACCGTACCTCAATATGCCCGCACAATCACACACATGAt > 2:324690/1‑251 (MQ=255) aaCTGGAAAGGGACAGTTACTGAAATCACC‑ACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAAGGGGCGCAGGCCCTACAGGACGCGGGCTCTGC‑AACCGATCCTCACTATGCCCGCAAAATAACCAACATGAt > 2:324705/1‑251 (MQ=255) aaCTGAAAAGGGCCAGTTACTGACATCAAC‑ACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGTCCAGGCCCTACACGACGCGGGCTATGCTCAC‑GCCCCTCCCTATGCCAGCAAACTCACCAACATGAt > 2:587338/1‑251 (MQ=255) atGGAAAGGGCCAGTTACTGAAATCACC‑ACGACTGAATATGAAAACGGCTAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGTATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTc < 1:156533/250‑1 (MQ=255) gggAAAGGGCCAGTTACTGAAATCACC‑ACGACTGAATATGAAAACGGCGAAGCAAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCCCGTATCTGGAAGCCTTGTCGGATTACGTGGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTca < 1:520783/250‑1 (MQ=255) aaGGTCCAGTTACTGAACTCACC‑CCGACTGAATATGAAAACGGCGAAGCGAACAAAGAAAAAGCGAGGTTTCGCGTCAACAGCCCGTATCTGGAAGCTTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTCCGCCGCCGTGACGCCCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTcagcag < 2:407098/251‑1 (MQ=255) ggCCAGTTACTGAAATCCCC‑CCGATAGAATATGAAAGCGGCGAAGCGAAGAAAGTAAAAGCGAAGTTCCCGGTCTACAGCTCGTATCTGGAAGCCTGGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATg < 2:488627/251‑1 (MQ=255) gAAATCACC‑ACGACGGAATAGGAAAACGGCGAAGCGAAGAAAGGAAATGCGAAGTTGCGCGTCGACAGCTCGTATCTGTAAGCCTTGTCGGATTCCGTTGGGCTGTGAACGC‑GTAACTCGCGCTACGCCGCCGGGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGGCCTACAGGACGCGGGGTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGAGTTAACAGATGAAATCGATAAg < 2:583441/251‑1 (MQ=255) aaTCACC‑ACGACTAAATATGAAAACGGCAAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCg < 2:423913/251‑1 (MQ=255) cc‑acGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGGCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAg < 2:164422/251‑1 (MQ=255) gAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGc < 1:517815/249‑1 (MQ=255) gAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCa > 1:486130/1‑250 (MQ=255) aaTATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCaaa < 1:250797/251‑1 (MQ=255) ggagaagcgaggAAAGTAAAAGGGAAGTTTCGCGACTACAGCTTGTTGCTGGAAGCTTTGCGGGATTACGTGGGGCTGTTACCGC‑GAAACTGGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAACCCTACAGTAt < 2:576056/248‑1 (MQ=255) gcgaagcgaaGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATg < 1:217431/251‑1 (MQ=255) aagcgaagAAAGGAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAAc > 2:462144/1‑251 (MQ=255) aGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATc > 2:226345/1‑251 (MQ=255) agggAAGTTTCGCGTCTACAGCTCTTATCTGGAAGCCTTGGCGGATTACGTGGGGCTGTTAACGC‑GTAACTCGCGCCACCCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATGCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTTATTATCTGTTCt < 2:350949/248‑1 (MQ=255) gCGAAGTTTCGCGTCTACATCTCGTATCGGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTg < 1:380061/251‑1 (MQ=255) cgTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCa > 1:77352/1‑249 (MQ=255) gTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGt > 2:94658/1‑251 (MQ=255) gTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGt > 1:152638/1‑251 (MQ=255) gTATCTGGAAGCCTTGTCGGATTACGATGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGAGGGTCg > 1:333180/1‑251 (MQ=255) tATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGc > 1:288560/1‑251 (MQ=255) aTCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCt > 2:114749/1‑251 (MQ=255) tCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCACGCGAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTACGGCGGGACGCtc > 1:566324/1‑250 (MQ=255) ggAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCACGTCCGGCGGGTCGCTGCCg > 2:469803/1‑251 (MQ=255) gaaagcttggccGGATTACGTTGGTCTTTTAACGC‑GGAACGCGCGCTACCCCGCCGTGCCGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGGCCGGCGTGTCGCTGcc < 2:265493/239‑1 (MQ=255) gTCGGATTACGTGGGGCTGTAACCGC‑GTAACTCGCGCACCCCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATAct < 2:486130/250‑1 (MQ=255) cGGATTCCGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATActct < 1:253343/250‑1 (MQ=255) cGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGTTAATACTCTg > 1:136763/1‑251 (MQ=255) cGGATTACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGGCGCTGCCGATAATACTCTg < 1:213801/251‑1 (MQ=255) atttcgtggggctttgaccgc‑gTAACGCGCGCTACCCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTa < 2:328580/232‑1 (MQ=255) tACGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAAt < 1:460624/250‑1 (MQ=255) aCGTTGGGCTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAAtt < 1:392259/250‑1 (MQ=255) gTTGGGTTGTTAACGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGaa < 1:574344/251‑1 (MQ=255) ggCTGTTAACGC‑GTAACTCGAGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCACGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGACTAACTCAAGTCCGGGGGGTCGCTGACGATAATACTCTGTAATTGAAGGCt > 2:189355/1‑251 (MQ=255) aaCGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCTATAATACTCTGGAATTGAAGGCTTATAAg < 1:165192/250‑1 (MQ=255) aaCGC‑GTAACTCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAg < 1:270005/250‑1 (MQ=255) | ATTTGATCCTCGCTCAGGCGGCACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACC‑ACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGC‑GTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGC‑CACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAG > NC_000913/1137885‑1138369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |