Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,015,891	C→T	intergenic (+9/+61)	rmf → / ← fabA	ribosome modulation factor/beta‑hydroxyacyl‑acyl carrier protein dehydratase/isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,015,891	0	C	T	100.0%	110.5 / NA	34	intergenic (+9/+61)	rmf/fabA	ribosome modulation factor/beta‑hydroxyacyl‑acyl carrier protein dehydratase/isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/16);  total (18/16)

CACATTTAGTAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGACCAGTGAAT  >  NC_000913/1015657‑1016132                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  cacaTTTAGTAATCACTGTTTTCTTTTCCTCCAGAAACCAGAATGAGGGAAACGAGGCATGAAGAGAGAAAAACGAGATCGACTGGAACGGGCCCATCAACGTGGTTATCAGGCCGGCATCGCCGGACGATCAAACGACATGTGTCCCTATCAGACGCTGACTAAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTACTGGCCTGATTCTGTCTTTTTAAAAAGAAACCTc                                                                                                                                                                                                                                   >  2:575700/1‑251 (MQ=255)   caTTTAGTAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACATCCg                                                                                                                                                                                                                                 >  2:136584/1‑251 (MQ=255)    aTTTAGTAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGc                                                                                                                                                                                                                                >  1:470226/1‑251 (MQ=255)                         tttcCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCtttt                                                                                                                                                                                                           >  2:413389/1‑251 (MQ=255)                                 aGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATActct                                                                                                                                                                                                   >  1:327422/1‑251 (MQ=255)                                 aGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATActct                                                                                                                                                                                                   <  1:501086/251‑1 (MQ=255)                                        aGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGGACATCAACGTGGTTAGCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGATGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCTGACAGGGTAGGAATGGGCTGATTCTGTCTTTTTAAAAATAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGAt                                                                                                                                                                                            <  1:553318/251‑1 (MQ=255)                                        aGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGAt                                                                                                                                                                                            <  1:208688/251‑1 (MQ=255)                                                           aTGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACg                                                                                                                                                                         >  1:441764/1‑251 (MQ=255)                                                            tGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGt                                                                                                                                                                        >  2:424537/1‑251 (MQ=255)                                                                    cAAAAACGAGATCGCCTGGAAAGGGCACATCAACGTGGTTATCAGGCCGGCATCCCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGAAGTAATGGCATGCTTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCCGATTATAATAAGAAGGCAGACGTATCCTgg                                                                                                                                                                 >  2:519131/1‑250 (MQ=255)                                                                          cGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGAc                                                                                                                                                          <  1:7497/251‑1 (MQ=255)                                                                             gaTCGCCTGGATCGTGCACATCAACGTGGTTATCAGGCCGACATCGCCGGACGCTCAAAAGGAAGATGTCCCTATCAGACGCTGAATCAAAGGGCACAATGGCTGGGAGACTGTCGATAAGCCATGGCGGACTGGGTAGTAGTTGCCTGATTCTGTCTTTTTAAAAAGAAACCGCGCCATTGCGTAGGTTTCGCCTTTTGATACTTTGTCTGATTATAAGCAGAAGGCAGACGTATCCTGGAACAGACCGa                                                                                                                                                       <  1:237137/251‑1 (MQ=255)                                                                                   cTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTc                                                                                                                                                  >  2:387439/1‑250 (MQ=255)                                                                                     ggAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAg                                                                                                                                                <  1:413389/250‑1 (MQ=255)                                                                                             cacaTCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGc                                                                                                                                       <  2:327422/251‑1 (MQ=255)                                                                                                    aCGTGGTTATCAGGCCGGCATCGACGGTCGCTCAACCGAAATGTGTCGCTATCAGACGCTGAATCAAAGGTCAAAATGGCTGGGAGGATGGCGAGAAGCCGTGGCGGACAGGGGAGTAATGGCCTGATTCTGTCTTTTTAACAAGAAACCTCCGCATTGCGGCGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGCAGGCCGACGTATCCTGGAACAGACCGACTTTCAGGTGGCTGGCgggtta                                                                                                                                 >  2:284665/1‑246 (MQ=255)                                                                                                                ggCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGGATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCa                                                                                                                    <  2:285438/251‑1 (MQ=255)                                                                                                                              gACGCTCAAAAGACATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGCACAGACCGCCTTTCAGGTCGCTGGCGGTATAGATCCGCCGCCCATCAACCCGCACTTc                                                                                                      >  2:79739/1‑251 (MQ=255)                                                                                                                                 gCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGTGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCGGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGcc                                                                                                   <  1:491078/251‑1 (MQ=255)                                                                                                                                     aaaaGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATcc                                                                                               <  1:424537/251‑1 (MQ=255)                                                                                                                                     aaaaGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATAAGACGACCATCAACCAGCACTTCGCCATcc                                                                                               >  1:349348/1‑251 (MQ=255)                                                                                                                                         gAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCa                                                                                           <  1:503404/251‑1 (MQ=255)                                                                                                                                                      aTCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAg                                                                              <  1:346447/251‑1 (MQ=255)                                                                                                                                                        cAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGACTTTTTAAAAAGAAACCTCAGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGTACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGGACTTCGCCATCCGCCAGGCCCCTAATCAgcc                                                                            >  2:397098/1‑249 (MQ=255)                                                                                                                                                                           cacaATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGtt                                                          <  1:136584/250‑1 (MQ=255)                                                                                                                                                                              aaTGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTGTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTaaa                                                      <  1:387439/251‑1 (MQ=255)                                                                                                                                                                                                            cGGACAGGGTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGATTACGGTAGGTCACTTTTTTCGCTGt                        >  2:188925/1‑251 (MQ=255)                                                                                                                                                                                                                  gggTAGTAATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGc                    >  2:140675/1‑249 (MQ=255)                                                                                                                                                                                                                    gtagtaATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGGTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGt                 >  2:416387/1‑250 (MQ=255)                                                                                                                                                                                                                      agtaATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTAcc              >  1:136698/1‑251 (MQ=255)                                                                                                                                                                                                                        taATGGCCTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTACGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTATCGCTGTCGGCAGTACCTg            >  1:188489/1‑251 (MQ=255)                                                                                                                                                                                                                                 gATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGACCAGTGaa   <  2:470226/251‑1 (MQ=255)                                                                                                                                                                                                                                   ttCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACTACGGTTAACAATGCGTTTAAAGTGAATTCGGTAGGGCACTTTTTTCGCTGTCGGCAGTACCTGACCAGTGAAt  <  2:546948/250‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  CACATTTAGTAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGACCAGTGAAT  >  NC_000913/1015657‑1016132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑