Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	881,531	A→T	I269F (ATC→TTC)	dacC →	D‑alanyl‑D‑alanine carboxypeptidase DacC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	881,531	0	A	T	97.6%	124.2 / NA	41	I269F (ATC→TTC)	dacC	D‑alanyl‑D‑alanine carboxypeptidase DacC
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (16/24);  total (17/24)
Fisher's exact test for biased strand distribution p-value = 4.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

GGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGCGAGTTATACGTTAACGGAACCGCAGCTTACCGCACCGCTGAAAAAAGGTCAGGT  >  NC_000913/881287‑881775                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     ggCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGtt                                                                                                                                                                                                                                                <  2:72545/251‑1 (MQ=255) ggCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTAATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGtt                                                                                                                                                                                                                                                <  1:133151/251‑1 (MQ=255)       gCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAAt                                                                                                                                                                                                                                           <  2:267519/250‑1 (MQ=255)              aaaGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAACCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTg                                                                                                                                                                                                                                    >  1:199714/1‑250 (MQ=255)                 gCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGGTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGAGATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGaa                                                                                                                                                                                                                                <  1:484027/251‑1 (MQ=255)                   aTTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAat                                                                                                                                                                                                                              >  1:277819/1‑251 (MQ=255)                      gATCAACGATGTGCCGGAAGAGTAAGCCATTCAAAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAAACGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGTTACCCAGGGCGATATGCGTTTAATCTCCGGAGTGTTGGGGGCGAAAACCGTCAGTTTCCGTTTTAATGAGTCTGAGAAattat                                                                                                                                                                                                                           <  1:436436/251‑1 (MQ=255)                                        agagTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGGTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGct                                                                                                                                                                                                         >  2:131136/1‑251 (MQ=255)                                                   ttCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAAcc                                                                                                                                                                                              >  2:417543/1‑251 (MQ=255)                                                          agagaaagagTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCAAGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGATGGGGGCGAAACCGGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGaggc                                                                                                                                                                                       >  1:516795/1‑248 (MQ=255)                                                               aagagTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATTTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAAtt                                                                                                                                                                                  <  1:25818/251‑1 (MQ=255)                                                                 gagTTCACCTTCAGCACAATTCGGCCGCCTAACCGTAACCGTCTGCTGCGGCCCAGCAATCTGAATGCTGATGGCATGAAAACCGGAACCACGGCAGGCACGGGCTATAATCTGGTTGCTTCGGCTACACAGGGCGATATGCGTTTAATCTCCGTAGTGATGGGGGCGAAAACCGAACGTTTCCCTTTTAATGAGTCTGCGAAATTATTGACCTGGGGTTTCCGCTTCTTTGACACCGTGCCGCCAATTa                                                                                                                                                                                 >  2:296961/1‑250 (MQ=255)                                                                                 aaatctggcAGCCTAACCGTATCTGTCTGCTGTGGAGCAGCAATCTGAATGTTGAGTGCATGAAGACAGGAACCACGGCATGCGCGGTATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTATTGTTGGGGGCTAAAACCGTCCGTTTCCGTTTTAATGATTCTGAGAAATAATTGACCTGGCGTTTCCTCTTCTTTGAAACCGTGACGCTAATTAAACCTGAtcccccctct                                                                                                                                                                <  2:433819/243‑10 (MQ=255)                                                                                 aaagtcggcAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCttt                                                                                                                                                                <  2:277819/243‑1 (MQ=255)                                                                                  aaTTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTtg                                                                                                                                                               <  1:117710/251‑1 (MQ=255)                                                                                   aTTCGCCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTtgt                                                                                                                                                              <  1:361708/251‑1 (MQ=255)                                                                                           gCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGTGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAgc                                                                                                                                                      <  2:481462/251‑1 (MQ=255)                                                                                                 ccgtaaccgtCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCt                                                                                                                                                >  1:492131/1‑251 (MQ=255)                                                                                                       ccgtCTGCTTTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCTAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCCCCTTTGTGACTCAGCGCGTCTGGtttt                                                                                                                                          <  2:317417/251‑2 (MQ=255)                                                                                                       ccgtCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTg                                                                                                                                          <  1:177356/251‑1 (MQ=255)                                                                                                              ctgTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATa                                                                                                                                    >  2:81985/1‑250 (MQ=255)                                                                                                                  ggAGCAGCAATCTGAATGTTGATGGCATGACGACAGGAACCACGGCCGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCCGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGt                                                                                                                                             >  1:521877/1‑237 (MQ=255)                                                                                                                  ggAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGTGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTGTAATGAGTCTTAGAAATTAGTGTCCTGGGGTTGCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGGGACCCAGCGCGGCTGGt                                                                                                                                             <  2:521877/237‑1 (MQ=255)                                                                                                                   gAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCg                                                                                                                              <  1:553155/251‑1 (MQ=255)                                                                                                                       agcaATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGt                                                                                                                          >  1:26764/1‑251 (MQ=255)                                                                                                                                aaTGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATg                                                                                                                                                                                                                                          >  2:196752/1‑130 (MQ=255)                                                                                                                                aaTGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATg                                                                                                                                                                                                                                          <  1:196752/130‑1 (MQ=255)                                                                                                                                aaTGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCgg                                                                                                                 <  1:401746/251‑1 (MQ=255)                                                                                                                                   gTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATACGAGCGAAGAGAATCTCgggg                                                                                                               >  1:83143/1‑250 (MQ=255)                                                                                                                                                 gACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCt                                                                                                >  1:401939/1‑251 (MQ=255)                                                                                                                                                                      gggATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGAGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGCCCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGCACATACCGCGTGGGc                                                                           >  2:556336/1‑251 (MQ=255)                                                                                                                                                                                       cTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGaa                                                           >  1:41329/1‑250 (MQ=255)                                                                                                                                                                                               aCCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAGCCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGCGAGt                                                    <  1:118128/249‑1 (MQ=255)                                                                                                                                                                                                 ccAGGGTGATATGCGTTGAAGCTCCGTGTTGTTGGGGGCGAAAACCGACCGTTGCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAGCCGTGACGCCAATTAAACCTGAGGCCACCTTTGTGACTCAGCGCGTGTGGGTTGGTGATAAGAGCGAAGTGAAGCTCGGGGCAGGTGAAGCGGGCGCAGTGACCATACCGCGTGGGCAGCGGAAAAACCTGAAAGCGAGTtata                                                <  2:471404/251‑1 (MQ=255)                                                                                                                                                                                                                 ttAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGCGAGTTATACGTTAACGGAACCGc                                 <  1:131136/250‑1 (MQ=255)                                                                                                                                                                                                                        ccGTAGTGTTGGGGGCGAAACCCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGCGAGTTATACGTTAACGGAACCGCAGCtt                            <  2:128782/248‑1 (MQ=255)                                                                                                                                                                                                                        ccGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGCGAGTTATACGTTAACGGAACCGCAGCt                             <  1:81808/247‑1 (MQ=255)                                                                                                                                                                                                                         cGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGCGAGTTATACGTTAACGGAACCGCAGCTTaccg                        <  1:56986/251‑1 (MQ=255)                                                                                                                                                                                                                                 tGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGCGAGTTATACGTTAACGGAACCGCAGCTTACCGCACCGCTg                >  2:402053/1‑251 (MQ=255)                                                                                                                                                                                                                                              cgaccgTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGCGAGTTATACGGTAACGGAACCGCAGCTTACCGCACCGCTGAAAAAAGGTCAgg   >  1:122442/1‑251 (MQ=255)                                                                                                                                                                                                                                               gaccgTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGCGAGTTATACGTTAACGGAACCGCAGCTTACCGCACCGCTGAAAAAAGGTCAGGt  <  1:556336/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     GGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGCGAGTTATACGTTAACGGAACCGCAGCTTACCGCACCGCTGAAAAAAGGTCAGGT  >  NC_000913/881287‑881775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑