Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	784,677	G→A	A49A (GCC→GCT)	zitB ←	Zn(2(+))/Cd(2(+))/Ni(2(+))/Cu(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	784,677	0	G	A	100.0%	158.8 / NA	47	A49A (GCC→GCT)	zitB	Zn(2(+))/Cd(2(+))/Ni(2(+))/Cu(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/31);  total (16/31)

CAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATT  >  NC_000913/784433‑784921                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     cAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATG‑TGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCa                                                                                                                                                                                                                                               <  2:453896/251‑1 (MQ=255)    ccACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAAt                                                                                                                                                                                                                                             <  1:207532/251‑1 (MQ=255)    ccACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAAt                                                                                                                                                                                                                                             <  1:523860/251‑1 (MQ=255)      ccGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCTTCAGCCAGCAATGc                                                                                                                                                                                                                                           <  2:115443/250‑1 (MQ=255)        ggCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCa                                                                                                                                                                                                                                         <  2:217395/251‑1 (MQ=255)         gCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAAGGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCag                                                                                                                                                                                                                                        <  2:337328/251‑1 (MQ=255)                ccATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGAGAGTAGGGCGAAAAGCAGGGCGGCAGTATCTGTCAACATATGAACCCAATCAGCCAGCAATGCCAGAGAAcc                                                                                                                                                                                                                                  >  1:541756/1‑250 (MQ=255)                    catcatGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGACCATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAAcc                                                                                                                                                                                                                                  >  2:583926/1‑246 (MQ=255)                    catcatGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAAcc                                                                                                                                                                                                                                  <  1:583926/246‑1 (MQ=255)                    catcatGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCagaa                                                                                                                                                                                                                              <  2:131746/250‑1 (MQ=255)                       catGCCGCCCTCGACCGGACGCGGAGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGAGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCagaaaga                                                                                                                                                                                                                           >  2:66694/1‑250 (MQ=255)                                   gACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACg                                                                                                                                                                                                               >  1:514061/1‑250 (MQ=255)                                      cgggcgcgGCGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACtt                                                                                                                                                                                                           <  1:327074/247‑1 (MQ=255)                                                gTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCAt                                                                                                                                                                                                 >  1:267375/1‑251 (MQ=255)                                                     gAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAAcc                                                                                                                                                                                            >  1:123837/1‑251 (MQ=255)                                                        gCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCgg                                                                                                                                                                                         <  1:560001/251‑1 (MQ=255)                                                          gTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGc                                                                                                                                                                                        >  2:329095/1‑250 (MQ=255)                                                                         aGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCAGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACACGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGc                                                                                                                                                                         >  2:478149/1‑250 (MQ=255)                                                                                 aaaaTGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATAcaacaa                                                                                                                                                                <  1:173690/251‑1 (MQ=255)                                                                                             aCCACGCGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCatt                                                                                                                                                    >  1:458678/1‑251 (MQ=255)                                                                                             aCCACGAGTGCTATGTCATGCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGGGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCATCATAATCCCGGCAGTCACGGCGAAGGCATacagcattcgtctagcctt                                                                                                                                                    <  2:56223/251‑17 (MQ=255)                                                                                               cACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCattat                                                                                                                                                  >  1:93328/1‑251 (MQ=255)                                                                                                           ggCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCa                                                                                                                                      <  2:39383/251‑1 (MQ=255)                                                                                                            gCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTAc                                                                                                                                                                                                       >  2:97564/1‑185 (MQ=255)                                                                                                            gCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTAc                                                                                                                                                                                                       <  1:97564/185‑1 (MQ=255)                                                                                                                                    gTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGATGTGAAAATTGCAAGGCGAGTAGGGCGAAAAGCAGGGCTGCAGTATCGGTCAACTTATGACCCGCATCAGCCAGGAATGCCAGATAACCAGAAAGAAAACCACCAACTGCGTCTACCAGCATAAGCCCGGCAGTCTCGCCGAAATCATTCAGCAAGCGTCGATCATTATTAGCTTCTGGCAGGTGTGAGGCTGTgtgtgagtgtga                                                                                                             <  2:524020/251‑1 (MQ=255)                                                                                                                                             aGCCAACCGAAAGGGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCAt                                                                                                    <  2:184389/251‑1 (MQ=255)                                                                                                                                                      aaaGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCa                                                                                           <  2:301244/251‑1 (MQ=255)                                                                                                                                                             ggCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCtt                                                                                     <  1:478149/250‑1 (MQ=255)                                                                                                                                                               cGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAat                                                                                  <  1:248476/251‑1 (MQ=255)                                                                                                                                                                 aaTGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAatta                                                                                <  1:488044/251‑1 (MQ=255)                                                                                                                                                                       gggAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCa                                                                          <  1:71770/251‑1 (MQ=255)                                                                                                                                                                         gaggaCGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTTAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACt                                                                        <  2:28851/251‑1 (MQ=255)                                                                                                                                                                          aggaCGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACtt                                                                       <  1:63532/251‑1 (MQ=255)                                                                                                                                                                              cgacgTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACt                                                                   <  1:473654/251‑1 (MQ=255)                                                                                                                                                                                       aaTTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGAcat                                                          <  2:123837/251‑1 (MQ=255)                                                                                                                                                                                        aTTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGCCCCGCATCAGCCAGCAATGACAGAGAACCAGCAAGAAAACCACCAACGACTTCTCCCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGAAGGAGTGAGGACGTGTGTGCGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGAcatc                                                         >  1:298879/1‑251 (MQ=255)                                                                                                                                                                                          tGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGAcatcat                                                       >  1:309859/1‑251 (MQ=255)                                                                                                                                                                                                gCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCt                                                 >  1:353000/1‑251 (MQ=255)                                                                                                                                                                                                      aGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGGCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTGCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCGTTTAg                                            <  2:267375/250‑1 (MQ=255)                                                                                                                                                                                                        ggcgacacgCAGGGCGGCAGTATCGGTCAACATATGAACCGCATCAGCCACCAATGCCAGAGAACCAGAAAGACAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGCAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGa                                           >  2:77641/9‑249 (MQ=255)                                                                                                                                                                                                         gCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACg                                                                                                >  1:167732/1‑195 (MQ=255)                                                                                                                                                                                                         gCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACg                                                                                                <  2:167732/195‑1 (MQ=255)                                                                                                                                                                                                         gCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGt                                        <  2:173551/251‑1 (MQ=255)                                                                                                                                                                                                                             aTCGGTCAACAGATGACCCGCAGCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCGTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTGATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGgagag                     <  2:364318/250‑1 (MQ=255)                                                                                                                                                                                                                                     aCATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGctc            <  2:436321/251‑1 (MQ=255)                                                                                                                                                                                                                                               cGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCattatt  <  2:581668/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     CAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATT  >  NC_000913/784433‑784921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑