Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	502,232	G→A	T336I (ACC→ATC)	ybaL ←	putative transporter YbaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	502,232	0	G	A	100.0%	191.1 / NA	60	T336I (ACC→ATC)	ybaL	putative transporter YbaL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/27);  total (33/27)

CAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTC‑TGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGG  >  NC_000913/501989‑502469                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              cAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTC‑TGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGACCAGTACCGGGTTGCGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGACATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGc                                                                                                                                                                                                                                        >  1:379693/1‑251 (MQ=255)  aaaTATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTC‑TGCTCTTCCAGCGTTTCGGTCTTCGACAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTACGGCCTGCGGCAGAAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGcc                                                                                                                                                                                                                                       >  1:32434/1‑251 (MQ=255)   aaTATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTC‑TGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCg                                                                                                                                                                                                                                      <  1:109837/251‑1 (MQ=255)      atCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTC‑TGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTAc                                                                                                                                                                                                                                   >  2:216514/1‑251 (MQ=255)             gggATCTGCTTCTCTTCTTCTATTGCCTCTTCCAGCGTC‑TGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTCCCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGa                                                                                                                                                                                                                            <  1:207625/251‑1 (MQ=255)                  cgtctgctcttctGCGATTGCCTCTTCCAG‑GTCCTGCTCTTCCAGCGTTTCTGTCTTCGCCGGAAAGTTCTCCAGTAGGGGGAACAGTACCGGGTTGAGCAAAATCGAAGGGATCGCCCCTCCCGGGTCCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATGCCCAGTCCC‑GCCAGGATAAACGCGAACGCGCCAATCTGCGGCAGGCTGGCGGCGATGATTGATGCCGTACGTTGGGAGTGa                                                                                                                                                                                                                        <  2:28070/244‑1 (MQ=255)                   tGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTC‑TGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGAcc                                                                                                                                                                                                                      >  1:293113/1‑251 (MQ=255)                            tcttcGTTTGCCTCTCCCAGCGTC‑TGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTCCTCCAGTATTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTCCCAGTTCCAGGTTTTCTCCGGCCTGCGGCAGTAAATTCAATGCCATGCCCAGTCCC‑GCCAGTATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGt                                                                                                                                                                                                               <  1:80612/249‑1 (MQ=255)                                  aTTGCCTCTTCCAGCGTC‑TGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCAcc                                                                                                                                                                                                         >  1:260073/1‑249 (MQ=255)                                     cccttttCCAGCGTC‑TGCCTTTCCATCTTTTTGGTCTCCTACAGATATTTCTCCAGTAGTTCGAACAGTACCGGGTTAACCATAATCGACAGGAGCGCCCCTGCCAGTCTCAGGTTTTGTCCGGCATGCGGCAGTAAATTCAATGCCATTCCCACTCCC‑GCCAGTATATACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTATTGCCGTACGGTGGGAGTTACCAAACAGTCTCACCAGGaa                                                                                                                                                                                                    <  2:380732/246‑1 (MQ=255)                                             cAGCGTC‑TGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCgg                                                                                                                                                                                            >  2:404114/1‑251 (MQ=255)                                               gCGTC‑TGCTCTCCCAGCGTTCCGGTCTCCGCCAGATATTTCTCCAGTAGTGCGAACATTACCGGGTTGAGCATAATCGACAGAATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCt                                                                                                                                                                                          <  2:293113/251‑1 (MQ=255)                                                 gTC‑TGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTa                                                                                                                                                                                         >  2:53987/1‑250 (MQ=255)                                                 gTC‑TGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCCCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTaa                                                                                                                                                                                        <  1:404114/251‑1 (MQ=255)                                                     tGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCg                                                                                                                                                                                             >  1:91503/1‑243 (MQ=255)                                                     ggCTCTTCCAGCGTTCCGGTCTTCGCCAGATATTTCGCCAGAAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCg                                                                                                                                                                                             <  2:91503/242‑1 (MQ=255)                                                         ctTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGCACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACtt                                                                                                                                                                                  >  2:368581/1‑250 (MQ=255)                                                         ctTCCAGCGTTTCGGTCTTCGCCAGATATTTCCCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAGCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACt                                                                                                                                                                                   <  1:193652/249‑1 (MQ=255)                                                                 gTTTCGGTCTGCGCCGGATTTTTCTCCAGTAGGGCAAAAAGTACCGGGTTGGGCATAATCGAAAGGATCGCCCCTGCCAGTCCCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAg                                                                                                                                                                         <  2:135862/251‑1 (MQ=255)                                                                     cGGTCTCCGCCAGATATTTCCCCAGTAGTGCGAAAAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGGCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGaat                                                                                                                                                                      <  2:202532/250‑1 (MQ=255)                                                                           tCGCCAGATATTTCTCCAGTAGGGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATc                                                                                                                                                                  <  2:7398/248‑1 (MQ=255)                                                                               cAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGCTCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATCCCATTCCCAGTCCC‑GCCCGGATACACGCGACCTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGCGTGACCAAACAGTCGCCCCAGCACAAATGCGGCTCACGACTTACCAAACAGAATAATCGCCAGcc                                                                                                                                                           >  1:296927/1‑250 (MQ=255)                                                                               cAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCg                                                                                                                                                           >  1:178140/1‑251 (MQ=255)                                                                                   tatTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGc                                                                                                                                                       >  1:163101/1‑251 (MQ=255)                                                                                       tatcCAGCAGTGCGAACCGTACCGCGTTGCGCATACTCGACAGGAACGCACCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GACAGGATAAACGCGAACTCACCAGTCTGCGCCAGGCTGGCGGCGATGATTCATGCCGTACGTTGAGAGTGACCAAACAGTCGCCCCATGAGCAATGCTGCTAACGACTTACCACACAAACTACTCGCCAGCGTCGCCAGc                                                                                                                                                   >  2:307521/3‑251 (MQ=255)                                                                                           cAGTAGTGCGAACATTACCGGGTTGAGCATAATCGCCAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTg                                                                                                                                               <  1:86469/251‑1 (MQ=255)                                                                                             ggagtGCGAACAGTACCGGGTTGTCAATAAGCGCAAGAATCCCCCGTGCCAGTCCCAGGTTTTGTCCGCCCTGTGGCAGTAAACGCAATGCCTTTCCCAGTCCC‑GCCATGATAAACGCGAACTCACCAAGCTGCGCCAGGCTGGCGGCGATGATTAATGCCTGACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAACAATCGCCAGCGTCGCCAGCGCTGcc                                                                                                                                             <  2:259265/249‑1 (MQ=255)                                                                                                    gAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCggat                                                                                                                                      >  2:428742/1‑249 (MQ=255)                                                                                                    gAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGtt                                                                                                                                      >  2:267513/1‑251 (MQ=255)                                                                                                    gAACAGTACAGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGACTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGCTAAGCGCGAACACACCAATCTGCGCCAGGCTGGCGGCGATGATTACTGCCGGACGTTGGGAGAGACAAAACAGTCGCCCCAGGCAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCACCGCCAGCACTGCAAGCGGtt                                                                                                                                      >  2:252075/1‑251 (MQ=255)                                                                                                          tACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGCTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACtt                                                                                                                                                                                  >  1:369239/1‑201 (MQ=255)                                                                                                           ccgggtttgggcatcatggccAGGATCACCCCTGCCAGTACCAGGTTTGGTCCGGCATGCGGCAGAAAATACAATGCCATTCCCAGTCCC‑GCCATGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGGCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAAt                                                                                                                               <  2:48928/231‑1 (MQ=255)                                                                                                                   gAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTa                                                                                                                       <  1:577856/251‑1 (MQ=255)                                                                                                                          aTCGACAGGATCGCCCCTGCCAGTCCCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATc                                                                                                                <  1:53987/251‑1 (MQ=255)                                                                                                                          aTCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGaac                                                                                                                >  1:489303/1‑249 (MQ=255)                                                                                                                                 ggATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAAt                                                                                                                                                                                                >  2:379995/1‑164 (MQ=255)                                                                                                                                 ggATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAAGCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAAt                                                                                                                                                                                                <  1:379995/164‑1 (MQ=255)                                                                                                                                   aTCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACAt                                                                                                       >  1:302445/1‑251 (MQ=255)                                                                                                                                     cgcactttccAGTACCAGGTTTTGTCCGGCCGGCGGCAGTAAATACAATGCCTTCCCCGGTCCC‑GCCAGGATAAACGCGAACTCTCCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAAACGCAAGGGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACTGCTCAaacaacccca                                                                                                     <  2:1705/243‑5 (MQ=255)                                                                                                                                              cAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAACCGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACgg                                                                                               >  2:251943/1‑248 (MQ=255)                                                                                                                                              cAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACgg                                                                                               >  1:237878/1‑248 (MQ=255)                                                                                                                                                 caccgggTTTGGTCCGGCCTGCGGCTGTAAATTCAAGGCCATTCCC‑GTCCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCGGAATCAGAATTAACGGATCAAACAACAGCCCGACGTAGACaa                                                                                         <  2:248839/246‑1 (MQ=255)                                                                                                                                                                      cGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCCCCAATCTGCGCCAGGCTAGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAAAAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACAACCAGACGGGGCAAAAAAACAGCACCGCCAAcgcg                                                                     >  1:540391/1‑250 (MQ=255)                                                                                                                                                                         cAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTcgc                                                                 >  1:553015/1‑251 (MQ=255)                                                                                                                                                                           gTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGCGCCAAAAAACAGCACCGCAAACGCGTCgcgc                                                               >  1:354739/1‑251 (MQ=255)                                                                                                                                                                              aaTTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAAt                                                            <  1:347201/251‑1 (MQ=255)                                                                                                                                                                                        cATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCaaa                                                                                                             >  1:251103/1‑192 (MQ=255)                                                                                                                                                                                        cATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAAGCAGAATTAACGGATCaaa                                                                                                             <  2:251103/192‑1 (MQ=255)                                                                                                                                                                                         agtCCCAGGCCC‑GCCAGGAAAAACGCGAACTCACCAATCAGCGACGGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGGCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCTCCAGCGTCGCCAGCGCTGCCAGCGGTTGCTGAATCAGAATTAACGGAGCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCATACGAGGCGCTCAATGGCAGCGtttc                                                 <  2:28240/249‑4 (MQ=255)                                                                                                                                                                                               aGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACCTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGggc                                           >  1:402172/1‑251 (MQ=255)                                                                                                                                                                                                        cAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGAAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACggg                                   >  1:282025/1‑250 (MQ=255)                                                                                                                                                                                                           gATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGAc                                <  1:53826/251‑1 (MQ=255)                                                                                                                                                                                                               aaCGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGCCAGCGTAACGTGGGCGGCACGGTGACTc                              >  2:128020/1‑249 (MQ=255)                                                                                                                                                                                                                 cgcgAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGtt                          <  1:101460/251‑1 (MQ=255)                                                                                                                                                                                                                    gAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGGATCAGAATTAACGGATCAAACAACATCCCGACGGCGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTc                         >  2:349664/1‑249 (MQ=255)                                                                                                                                                                                                                    gAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGAGACTCAGTTc                         >  1:469049/1‑249 (MQ=255)                                                                                                                                                                                                                    gAACTCACCAATCTGCGCCAGGCTGGCGGCGAGGATTAATGCCGTACGGTGGGAGTGACCAAACAGTCGCACCAGGAAAAAGGCGGCTAACGACTTGCCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATGAACGGATCAAACAACATCCCGACGGAGAAAGAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAg                       <  1:68780/251‑1 (MQ=255)                                                                                                                                                                                                                              aTCTGCGCCAGGCTGGCGGCGATGATTACTGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGCATCGTTc               >  2:485548/1‑249 (MQ=255)                                                                                                                                                                                                                                         gCTGGCGGCGATGATTAATGCCGGACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAAGTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGGTCAGACTCGGTCAGTACCATCCCgg  <  2:565228/251‑1 (MQ=255)                                                                                                                                                                                                                                         gCTGGAGGCGATGATTAATGCCGTACGTTGGGAGAGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAAAAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATAAGAATTAACGGATAAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGGGCATCAGTTCAGACTCGTTCAGTACCATCCCgg  >  2:232684/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              CAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTC‑TGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCC‑GCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGG  >  NC_000913/501989‑502469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑