Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	390,087	G→A	intergenic (‑360/‑164)	hemB ← / → yaiT	porphobilinogen synthase/putative autotransporter YaiT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	390,087	0	G	A	100.0%	81.9 / NA	24	intergenic (‑360/‑164)	hemB/yaiT	porphobilinogen synthase/putative autotransporter YaiT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/11);  total (13/11)

AAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTAGCGCGAAACATAATATGTTTTCTATGCATTGATAATTGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGC  >  NC_000913/389855‑390333                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       aaaCAGCAATAATTTTATATTCACTGAAAATATTTTTAATATTTATTTATAGCTGATGGTTATTATTTATTGTAGTTTGTTTTCGCTGCTATAAATTGAATAATTAAAATTTTGTTGCATATTCATAGGATATTTATATGATTTATGTTTGTGATATTTATTATGCGTATACTTAACAAACAAATTTATATGCTACATGTTGAACTACTGTGCTTTTATCACTTTAATGCGTATTTTTCTCCAttacttat                                                                                                                                                                                                                                      >  2:496913/1‑244 (MQ=255)                                  ttttAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTATTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGaat                                                                                                                                                                                                      >  1:254565/1‑250 (MQ=255)                                          tttatttaTAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATCAATTGAATAATTAAAACTTTGTTGCATATTCATAGAATATTTATCTGGTTTGTGTTTGTTATATTTATTATCCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTAATGCGTATTTTTCTCCATAAATTATATATGTCAACATTCGTACTTAGGGGAAAGAATAATTGaccc                                                                                                                                                                                             >  1:302842/1‑248 (MQ=255)                                                    gCTGTTGGTTATTATTGTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGGATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATGTGATTCGTATTGTTCTGCATAAATTATATATGTCCACATTCGGCCTTAGGGGAAAGAAGAATTGAACCATTTGTCTGt                                                                                                                                                                                   <  2:378885/251‑1 (MQ=255)                                                          ggTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTATTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTTATCCATTCGTCTGTAActca                                                                                                                                                                             <  2:373973/251‑4 (MQ=255)                                                                                        gctATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTATTTTTCTCCATAAAGTATATATGTCCACATGCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTAGCGCGAAACATAATATg                                                                                                                                                <  2:19232/250‑1 (MQ=255)                                                                                                   aataatTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTATTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTAGCGCGAAACATAATATGTTTTCTATGCAt                                                                                                                                    >  1:181128/1‑251 (MQ=255)                                                                                                   aataagTAAAACTTTGTTGCATATTCATAGGAGATGTATCTTGTTTGTGGTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATATTGTGCTTGTATGAATTTGATGCGTATTTTTCGCCATAAATTATATATGTGCACATTCGGACTTAGGGGAAAGAATAATTGATCCATTCGTCTGTAACGCAGGAGAATCGTGAGCGCGAAGCATAATATGTTTTTTATGCAt                                                                                                                                    <  2:538852/251‑1 (MQ=255)                                                                                                             aCTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTATTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTAGCGCGAAACATAATATGTTTTCTATGCATTGATAATTGa                                                                                                                          <  2:254565/251‑1 (MQ=255)                                                                                                                  gttgCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTATTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTAGCGCGAAACATAATATGTTTTCTATGCATTGATAATTGATGGAt                                                                                                                     >  1:308208/1‑251 (MQ=255)                                                                                                                     gCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTATTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTAGCGCGAAACATAATATGTTTTCTATGCATTGATAATTGATGGATCaa                                                                                                                  <  2:331058/251‑1 (MQ=255)                                                                                                                             aTAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTATTTTTCTCCATAAATTATATATGTCCACGTTCGGACTTAGGGTAAAGAATAATTGAACCATTAGTCTGTAACGCAGCATAATCGTTAGCGCGAAACATAATATGTTGGCTATGCAATGATAATTGATAGATCAACTTATTAc                                                                                                          <  2:204343/251‑1 (MQ=255)                                                                                                                             aTAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGAGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTATTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTAGCGCGAAACATAATATGTTTTCTATGCATTGATAATTGATGGATCAACTTATTAc                                                                                                          >  2:422847/1‑251 (MQ=255)                                                                                                                                     tttATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTATTTTTCTCCATACATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTAGCGCGAAACATAATATGTTTTCTATGCATTGATAATTGATGGATCAACTTATTACGTCCCTg                                                                                                   >  2:483225/1‑250 (MQ=255)                                                                                                                                                          atatTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTATTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTAGCGCGAAACATAATATGTTTTCTATGCATTGATAATTGATGGATCAACTTATTACGTCCATGAGGAGGGATGACAAATGCACTc                                                                             >  2:51563/1‑251 (MQ=255)                                                                                                                                                                 ttatGCGTATGCTTCAAAAACCAAATTATCTGCAACATGTTGAAATACTGTGCATTTATGAATTTGATGCGTATTTTTCTCCATAAATTATATATGTCCACATTCGTACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGATAGCGCGAAACATAATATGTTTTCTATGCATTGATACTTGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCCTTCCTGGaa                                                                       >  1:78749/1‑250 (MQ=255)                                                                                                                                                                         aTGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTATTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTAGCGCGa                                                                                                                                                           <  1:163602/158‑1 (MQ=255)                                                                                                                                                                         aTGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTATTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTAGCGCGa                                                                                                                                                           >  2:163602/1‑158 (MQ=255)                                                                                                                                                                           gCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTATTTTTCTCCATAAATTATATATGTCCACATTCGGCCTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTAGCGCGAAACATAATATGTTTTCTATGCATTGATAATTGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACttt                                                            >  2:247472/1‑250 (MQ=255)                                                                                                                                                                           gCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTATTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGTGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTAGCGCGAAACATAATATGTTTTCTATGCATTGATAATTGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGTACTCCTGGAAAAAGAAACtt                                                             <  1:226423/250‑1 (MQ=255)                                                                                                                                                                                       aaaTTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTATTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTAGCGCGAAACATAATATGTTTTCTATGCATTGATAATTGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAAt                                                <  1:247472/251‑1 (MQ=255)                                                                                                                                                                                                                       tttATGAATTTGATGCGTATTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTAGCGCGAAACATAATATGTTTTCTATGCATTGATAATTGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACctct                >  1:302543/1‑251 (MQ=255)                                                                                                                                                                                                                                   aTGCGTATTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTAGCGCGAAACATAATATGTTTTCTATGCATTGATAATTGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATgca    <  2:181128/251‑1 (MQ=255)                                                                                                                                                                                                                                     gCGTATTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTAGCGCGAAACATAATATGTTTTCTATGCATTGATAATTGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATgcagc  >  2:96926/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       AAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACTGTGCTTTTATGAATTTGATGCGTGTTTTTCTCCATAAATTATATATGTCCACATTCGGACTTAGGGGAAAGAATAATTGAACCATTCGTCTGTAACGCAGCATAATCGTTAGCGCGAAACATAATATGTTTTCTATGCATTGATAATTGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGC  >  NC_000913/389855‑390333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑