Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	367,812	G→A	G203G (GGC→GGT)	mhpR ←	DNA‑binding transcriptional activator MhpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	367,812	0	G	A	98.4%	201.0 / ‑4.3	65	G203G (GGC→GGT)	mhpR	DNA‑binding transcriptional activator MhpR
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (30/32);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.07e-01

GTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGA  >  NC_000913/367566‑368049                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              gTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACcgc                                                                                                                                                                                                                                           <  1:458740/251‑1 (MQ=255)      gtcccagtacaTGGTGTCAACGTAAATGCATGCCGTTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCACGCCCTTCTTCGAGCTGTTTTGCTGCCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTTCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTa                                                                                                                                                                                                                                       <  2:489852/240‑1 (MQ=255)      gcgcCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTa                                                                                                                                                                                                                                       <  2:195027/250‑1 (MQ=255)                  ggtctCAACGTCAATGCATTCCGCTAAGCCTTCCTGCCACCAGAATAGCCTGCGATTCAACCCCTTCGTCGATCTGTTTGACTCCCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGGTCACTGCGCAGCGGTACGGCAATAGAGGCCATCTTCTCCTCCTGATCCCATCCGCGGTAGTTCTGTCCGTaa                                                                                                                                                                                                                          <  1:496536/247‑1 (MQ=255)                     gtCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAAcc                                                                                                                                                                                                                        <  1:270673/250‑1 (MQ=255)                       cAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCctc                                                                                                                                                                                                                     <  2:67560/251‑1 (MQ=255)                            tAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTgc                                                                                                                                                                                                                >  2:184390/1‑251 (MQ=255)                               atgcatgcCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcg                                                                                                                                                                                                             <  2:112569/251‑1 (MQ=255)                               atgcatgcCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcg                                                                                                                                                                                                             >  1:523738/1‑251 (MQ=255)                                 gcatgcAGCTTCGCCTTCCAGCCACCAGAATAGCCGGAGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGCTGCTTTTACGCTGCCTGTTCAATGGTCATAGCGCTCGCCATATACACAAGATTCAGACAGCCAATCACCCGTTTTTCACTGCGCAGCGGTACGGCGATAGAGGCAGTATTCTCCTCCTGATACCAACCGCGGTAGTTCTGGCCGTAACCCTCTTTgcccgcg                                                                                                                                                                                                           >  2:375449/1‑251 (MQ=255)                                    tgcCGCTTCGCCTTCCGGCCACCAGAATAACCTGCGATTCAACCCCTTCTTCGCTCTGTTTTGCTACCCGTTGTAGCGCCGGACGCTGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCTCCATATACACCAGATTCAGCCAGCCAATCACCCGGTGTTCCCTGCGCAGCAGTCCGGCGATAGACGCGATCTTCTCCTCCTGATCCCAACCGCGTGAGTTCTGTCCGTAACCCCCTTTgcgctcgcgc                                                                                                                                                                                                        >  2:541158/1‑251 (MQ=255)                                     gcCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcgcgcg                                                                                                                                                                                                       >  2:441341/1‑251 (MQ=255)                                               cctCCGGCCACCTGTATAGCCTGCGATTCAACCCCTCCTTCGATCTGTTGTGCTACCCGTTGTATCGCCTGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACTGCCAATCTCCCGGTGTTCACTGCGCAGCGGTACGGCGATAGAGTCGATCTGCTCCTCCTGATCCCGGCCGCGGTAGTTCGGTCCGTAGCCGGCTTTgcgcgcgcgctcacgaatggc                                                                                                                                                                                             <  2:250239/249‑12 (MQ=255)                                                ttCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCt                                                                                                                                                                                            >  1:230301/1‑251 (MQ=255)                                                 tCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGTTCTGTTTTGCTACCCGTTGGAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTTGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCTCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCt                                                                                                                                                                                            <  1:266266/250‑1 (MQ=255)                                                     gCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCAGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGACAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCCACCGCGGTAGTTCTATCCGTAACCCTCTTTGCGCGCGCGCGCCAGCATGGCTTcc                                                                                                                                                                                         >  1:582533/1‑249 (MQ=255)                                                       cgccaGCATAGCCTGCGCTTCAACCCCCTCCTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTCCCGCTGCCTGTGCAATGCTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAAGCACCCGTTGTTCACTGCGCAGCGGTCCTGCGATAGAGGCGAGCTTCTTCACCTGATCCCAACCGCGTTAGCTCTGTCCGTAGCCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCt                                                                                                                                                                                     <  1:300348/249‑1 (MQ=255)                                                                        aTTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGcc                                                                                                                                                                    <  1:158444/251‑1 (MQ=255)                                                                                ccTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTACCGGTTCACGTGCCAGTTGAt                                                                                                                                                             >  2:211456/1‑250 (MQ=255)                                                                                  ttcttcGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGAtcgt                                                                                                                                                          >  2:467719/1‑248 (MQ=255)                                                                                   tcttcGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtc                                                                                                                                                         >  1:323002/1‑251 (MQ=255)                                                                                          tCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATCGTCCTCACCg                                                                                                                                                  >  2:265999/1‑251 (MQ=255)                                                                                          ggtGTTTTGCTACCCGTTGTAGGGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCAGTGCGCTCGCCATATAGACCAGATTCAGACAGCCAATCAGCCGTGGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTGCTGTCCGGAACGCTCGTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCg                                                                                                                                                  <  1:531319/249‑1 (MQ=255)                                                                                                       ccGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCCCCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTa                                                                                                                                     <  2:5248/251‑1 (MQ=255)                                                                                                         gttctAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCTCCCGTTGTTCACTGCGCAGCGGTACGGCGATATAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCTCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGGTCCCGTGCCAg                                                                                                                                                                  <  2:432299/216‑1 (MQ=255)                                                                                                         gTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATATAGGCGATCTTCTCCTCCTGATCACAACCGCGGTAGTTCTGTCCGTAACACTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAg                                                                                                                                                                  >  1:432299/1‑220 (MQ=255)                                                                                                         gTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAAc                                                                                                                                   >  2:125499/1‑251 (MQ=255)                                                                                                              aGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTc                                                                                                                              >  2:462856/1‑251 (MQ=255)                                                                                                              aGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCGCGCCATATACACCAGATTCAGACAGCCAATCGCCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCGTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGGCATCACCGGGGCGGGAGGCTAACATTTc                                                                                                                              <  2:367505/251‑1 (MQ=255)                                                                                                                       aGATTCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGTCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATATTCTCCTCCTGATCCCACCCGCGGTAGTTCTGTCCGTAAACCTCTTTGCTCGCGCGCGCCAGAATGGCTTCTAGCTTTAACGGTTCCCGAGCCAGATGATAGTCCTCAACGGGGCGGGAGGCCAACCTTTAGACTAAttt                                                                                                                     >  2:359427/1‑250 (MQ=255)                                                                                                                            cTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGc                                                                                                                >  1:340343/1‑251 (MQ=255)                                                                                                                                tCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTACTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTc                                                                                                            >  2:2687/1‑251 (MQ=255)                                                                                                                                  cGCTGCCTCTTAAATGGTCATTACGCTCGGCATATACACAAGATTCAGACAGCCAATCACCCGTTGTTCCCTGCGCAGCGGTACGGCGATAGAGGAGATCTTCTCCTCCTGATCCCAACCGCGGTAGCTCTGTCCGTACCCCTCTTTGCGCGAGAGCGCCAGAATGGCTTCCAGCTTTCACGCTTCCCGTGCCAGTTGGTAGTCATCACCGGCGCGAGAGGCTAACATTTCCTTTAATTCCtttccgactt                                                                                                          >  2:214999/1‑243 (MQ=255)                                                                                                                                      gCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCTATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGt                                                                                                        <  1:367649/249‑1 (MQ=255)                                                                                                                                        cTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCg                                                                                                    >  1:73286/1‑251 (MQ=255)                                                                                                                                          gTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCg                                                                                                    <  2:511978/249‑1 (MQ=255)                                                                                                                                            tCAATGGTCATTGCGCGCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGc                                                                                                 <  1:487435/250‑1 (MQ=255)                                                                                                                                                        gcgcTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagcca                                                                                    <  1:184390/251‑1 (MQ=255)                                                                                                                                                        gcgcTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTGCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagcc                                                                                     <  1:432872/250‑1 (MQ=255)                                                                                                                                                           cTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATATTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGt                                                                                 >  1:79427/1‑251 (MQ=255)                                                                                                                                                             cGCCATATACTCCAGATTCAGATAGCCAATCACCCGTGGGTCACTGCGCAGCGGTACGGCGATAGACGCGATCTTCGCCTCCTGATCCCAACTGTGGTAGTTCTGTCCGTAACTCTCGGTGCGCGTGCGCGCCAGAATGGATTCCAGCTTTAACGGCGCCCGTGCCAGTGGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCa                                                                               <  1:541158/251‑1 (MQ=255)                                                                                                                                                             cGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTGGTTCCGGGCAAAAGGCCAGCCAGGt                                                                                 <  2:79427/249‑1 (MQ=255)                                                                                                                                                                  tataCACAAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATCGAGGAGCTCTTCTCCACCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCCAAAGGCCAGCCAGGTccagccc                                                                          >  2:248675/1‑245 (MQ=255)                                                                                                                                                                   ataCACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGccc                                                                          <  1:193355/250‑1 (MQ=255)                                                                                                                                                                   ataCACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCg                                                                         <  1:441341/251‑1 (MQ=255)                                                                                                                                                                       ccgaGATTCAGACAGCCAATCACCCGTTGTTCCCTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCTCGGTAGTTCTGCCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCGTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATGAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAgga                                                                     <  1:2881/248‑2 (MQ=255)                                                                                                                                                                        ccatactcataCAGCCAATAACCCTTTGTACGCTGCGCAGCGGTACGGAGATAGAGGTGCTCTTCTCCCCCTGGGCCCAACCGCGGTAGTTCGGTACGTAACCCTCTTTGCG‑G‑GCGCGCCAGAATGGCTGCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGCCACCACCGGGGCGGGAGGCTAACATAGCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGt                                                                  <  2:313416/241‑1 (MQ=255)                                                                                                                                                                               cAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCCGTCTTGTTCCGGGCAAAAGGCCAGCCAGATCAGGCCCGGGGCGGttttt                                                              >  1:421788/1‑249 (MQ=255)                                                                                                                                                                               cAGACAGCAAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCCGCCAGGTCAGGCCCGGGGCGGGTTTc                                                              >  2:267134/1‑250 (MQ=255)                                                                                                                                                                                      ccAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCgg                                                      <  1:267134/251‑1 (MQ=255)                                                                                                                                                                                      ccAATCACCCGGTGTTCACTGCGCAGCGGTCCGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCGCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCgg                                                      <  2:323002/251‑1 (MQ=255)                                                                                                                                                                                        aaTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGc                                                     >  1:263450/1‑250 (MQ=255)                                                                                                                                                                                            aCCCGTTGTTCACTGCGCAGCGGTACGGCGATAGCGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTTAGGAGCGGGAAACg                                                >  2:382257/1‑251 (MQ=255)                                                                                                                                                                                                 ttgttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTTAGAAGCGGCAAAccacgcc                                           >  1:257362/1‑245 (MQ=255)                                                                                                                                                                                                  tgttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGGAGCGGCAAACGTCGccc                                          >  1:140051/1‑251 (MQ=255)                                                                                                                                                                                                  tgttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGAAAACGTCGccc                                          >  1:555143/1‑251 (MQ=255)                                                                                                                                                                                                     tCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTGGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGa                                        <  2:263450/250‑1 (MQ=255)                                                                                                                                                                                                      cACTGCGCAGCGGTACGGCGATAGAGGCGATATTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCCAACGTCGCCCGAcc                                      >  1:493165/1‑251 (MQ=255)                                                                                                                                                                                                                  gTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGa                          <  1:125499/251‑1 (MQ=255)                                                                                                                                                                                                                    aCGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTTCGAAGCGGCAAACGGCGCCCGGCAAATGCCCggggga                         >  2:548658/1‑246 (MQ=255)                                                                                                                                                                                                                            agagGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGc                <  1:462856/251‑1 (MQ=255)                                                                                                                                                                                                                             gagGCGATCCTCTCCTCCTGGACCCAACCGGGGTTGGTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCg               <  2:163076/251‑1 (MQ=255)                                                                                                                                                                                                                                          cctcctGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGGTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATGCCTTGCGGTCTTGTGCCGGGCAAAAGGCCATCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGa  <  1:274455/251‑1 (MQ=255)                                                                                                                                                                                                                                          cctcctGATCCCAACCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGCGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGCCGCCCCACCATTGCCCGGTGAAAGGATAAGCGGCGGaaaaggcgg  >  1:361410/1‑245 (MQ=255)                                                                                                                                                                                                                                          cctcctGATCCCAACCGCGGTAGTTCGGTCCGTAACCCCCTGTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACAGTGCGAGTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCGAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTTAAACGGTGa  <  1:342666/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              GTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGA  >  NC_000913/367566‑368049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑