Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	365,307	G→A	F333F (TTC→TTT)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	365,307	0	G	A	99.0%	335.5 / NA	99	F333F (TTC→TTT)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (44/54);  minor base C (1/0);  total (45/54)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGT  >  NC_000913/365072‑365545                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                ccaccaCATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCa                                                                                                                                                                                                                                   <  2:148013/250‑1 (MQ=255)  caccccATACAGGCCGTAGCGGTCGCACAGCGTGTTCCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCAGCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAgg                                                                                                                                                                                                                                 <  1:384348/251‑1 (MQ=255)      acaTAGAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAAAGCGAACAGCGCACGGCGGTAAAGATGTTCTGCTTCATCAGCAGGATATACTGCACCAGCGTCAGCTCATCCATGACCTGACCATGCAGAGGATGAAGCTCGTGTCGGTTAACGCTTCGTATTAGCAACGGCTTGCCGAT‑CAGCAGCAGCAGCCCATTTTCAATCCGAACCTCGCGAAAACCGAAATCGCAggactc                                                                                                                                                                                                                             >  2:280930/1‑247 (MQ=255)        aTACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCCTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTg                                                                                                                                                                                                                           <  1:162628/251‑1 (MQ=255)          aCAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCt                                                                                                                                                                                                                         <  2:102793/251‑1 (MQ=255)          aCAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGAACCTCGCGAAAACCGACATCGCAGGCTTCTGCt                                                                                                                                                                                                                         >  1:388156/1‑251 (MQ=255)            aGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTc                                                                                                                                                                                                                       <  1:37289/251‑1 (MQ=255)             ggCCGTAGCGGTCGCACAGCGTTTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGGTGTTCTGCTTCAGCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGAAACGGCTTGCCGTT‑CAGCAGCAGCAGACCAGTTTCAATCCGCACCGCGCGAAAACCGACATCGCAGGCTTCTGCTTCa                                                                                                                                                                                                                      <  2:479187/251‑1 (MQ=255)             ggCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCa                                                                                                                                                                                                                      <  1:57050/251‑1 (MQ=255)                cGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATc                                                                                                                                                                                                                   <  2:28357/251‑1 (MQ=255)                 gTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATc                                                                                                                                                                                                                   >  2:101402/1‑250 (MQ=255)                 gTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATc                                                                                                                                                                                                                   >  2:11341/1‑250 (MQ=255)                     cGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGt                                                                                                                                                                                                              <  2:528393/251‑1 (MQ=255)                      ggTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCCTCAGCAGGATATCCTGCACCATCGTCTGCTCGTCCATGACCTGCCCATGCAGAGGATGATGCTCGTGACGGTTAAAGACTCGCATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCCATCAACGTg                                                                                                                                                                                                             >  2:362803/1‑251 (MQ=255)                          gCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGt                                                                                                                                                                                                         <  1:26494/251‑1 (MQ=255)                           cacaGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAACACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTc                                                                                                                                                                                                        >  1:568140/1‑251 (MQ=255)                             caGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTcgg                                                                                                                                                                                                      <  1:524356/251‑1 (MQ=255)                             caGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTcgg                                                                                                                                                                                                      >  2:82559/1‑251 (MQ=255)                             caGCGGGTGCCACAGCGGATGGTTCGGATAATGCGAAGAGCGCACGGCGGTAAAGTTGTTCTGCTTCATCAGCAGGATAGCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTcgg                                                                                                                                                                                                      <  2:48043/251‑1 (MQ=255)                               gCGTGTACCACAGCGGATGGTTCGGATACTGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTcggcg                                                                                                                                                                                                    >  2:306971/1‑251 (MQ=255)                                   gtACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTGCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGGTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCATCAGCAGACCAGTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGgtgt                                                                                                                                                                                                <  1:575238/251‑1 (MQ=255)                                      ccACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGCCGGCGGTGTG‑c                                                                                                                                                                                             >  2:382599/1‑250 (MQ=255)                                       cacaGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑c                                                                                                                                                                                             >  1:396796/1‑249 (MQ=255)                                        acaGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGt                                                                                                                                                                                          <  1:91981/251‑1 (MQ=255)                                          aGCGGATGGTTCGGAGAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTc                                                                                                                                                                                        <  2:258481/251‑1 (MQ=255)                                            cGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑AAGTTCa                                                                                                                                                                                       >  1:303404/1‑250 (MQ=255)                                             tgATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAac                                                                                                                                                                                     >  2:63342/2‑251 (MQ=255)                                                     cGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCa                                                                                                                                                                              <  1:518882/250‑1 (MQ=255)                                                      ggATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACg                                                                                                                                                                            <  2:117415/251‑1 (MQ=255)                                                             gCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTCCAACCACCGCACGATAgaga                                                                                                                                                                     <  1:144935/251‑1 (MQ=255)                                                               gAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCAGTCGGCGGTGTG‑CAATTCAACCACCGCACGATagagctt                                                                                                                                                                   >  2:353153/1‑248 (MQ=255)                                                                  cAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCgg                                                                                                                                                                <  2:111869/251‑1 (MQ=255)                                                                       cACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGAttt                                                                                                                                                           <  1:316481/251‑1 (MQ=255)                                                                         cGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGCTATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCTGGATTTCg                                                                                                                                                         >  1:466607/1‑251 (MQ=255)                                                                            cGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGgcg                                                                                                                                                      <  2:31755/251‑1 (MQ=255)                                                                            cGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGgcg                                                                                                                                                      >  1:505876/1‑251 (MQ=255)                                                                            cGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGGCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGgcg                                                                                                                                                      <  1:64148/251‑1 (MQ=255)                                                                             gTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGgcgc                                                                                                                                                     <  1:227044/251‑1 (MQ=255)                                                                              ttAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCt                                                                                                                                                    >  2:468080/1‑251 (MQ=255)                                                                               tAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCt                                                                                                                                                    <  1:194545/250‑1 (MQ=255)                                                                                      gttcggcgtcatcaGCAGGATATCCTGCACCATCGGCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTGGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCGCCTCGCGAAATCCGTCATCGCAGGCTTCTGCTTCAATCAGCGGGCCGTCGGCTGTGTG‑CAGTGCAACCACTGCACGGTGGAGGTTCGGGATTTCGGGGCTCCGCAGtt                                                                                                                                            <  2:578589/243‑1 (MQ=255)                                                                                      gttCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGtt                                                                                                                                            <  2:12496/251‑1 (MQ=255)                                                                                          tGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGAACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCgg                                                                                                                                        >  2:159619/1‑251 (MQ=255)                                                                                            cTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCCTGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGG‑GTGGCAGTTCAACCACCGCACGGTAGAGATTCGGGATTTCGGCGCCCCCCAGTTTCgagt                                                                                                                                      >  2:216552/1‑248 (MQ=255)                                                                                                   agcagGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTc                                                                                                                                                          >  1:255502/1‑224 (MQ=255)                                                                                                   agcagGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGAc                                                                                                                               <  2:419061/251‑1 (MQ=255)                                                                                                   agcagGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTGCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTc                                                                                                                                                          <  2:255502/224‑1 (MQ=255)                                                                                                    gcagGATATCCTGCACCATCGTCTGCTCATCCCTGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCGCCACAGTTTCGGGTTTTCGACg                                                                                                                              >  2:57554/1‑251 (MQ=255)                                                                                                        gATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGAt                                                                                                                                                                          <  2:261524/203‑1 (MQ=255)                                                                                                        gATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGAt                                                                                                                                                                          >  1:261524/1‑203 (MQ=255)                                                                                                         atatCCTGCCCCATCGTCTGCTCATCCATGACCGGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAg                                                                                                                         <  2:396796/251‑1 (MQ=255)                                                                                                         atatCCTGCCCCACCGTCTGCTCATCCAAGACCTGACCATGCAGCGGATGATGCTCGTGCCGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGCCCATTTTCAATCCGCACCTCGCGACCACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGCTAGAGATTAGGGATTTCGGAGCTCCCCAGTTTCGGGTTTTCGAcctcc                                                                                                                           >  2:301904/1‑245 (MQ=255)                                                                                                                gCACCATCTTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAg                                                                                                                   <  2:473381/250‑1 (MQ=255)                                                                                                                     aTCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGAc                                                                                                             >  1:34504/1‑251 (MQ=255)                                                                                                                     aTCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATGCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGAc                                                                                                             <  2:382295/251‑1 (MQ=255)                                                                                                                       cGTCTGCTCATCCATGACCTGAACATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGGGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCGCCCCAGTTTCGGGTTTTCCACGTTCAGACGTAGTGTGAcgc                                                                                                           >  2:61334/1‑251 (MQ=255)                                                                                                                        gTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGAcgcg                                                                                                          >  1:547884/1‑251 (MQ=255)                                                                                                                         tCTGCTCATCCATGAACTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTAGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CCGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGAcgcg                                                                                                          >  2:84063/1‑250 (MQ=255)                                                                                                                         tCTGCTCATACATGACATGACAATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGACAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCCCGATAGAGATTCGGGGTTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCCGACGTAgtgt                                                                                                                >  2:544231/1‑244 (MQ=255)                                                                                                                         tCTGCTCAGCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTGCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAgtgt                                                                                                                <  1:544231/244‑1 (MQ=255)                                                                                                                          cTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGCTTTCGGCGCTCCACAGTTTCGGGGTTTCGACGTTCAGACGTAGTGTGACGCGAt                                                                                                        >  2:23371/1‑251 (MQ=255)                                                                                                                           tGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATc                                                                                                       >  1:505193/1‑251 (MQ=255)                                                                                                                            gCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCg                                                                                                      >  1:2695/1‑251 (MQ=255)                                                                                                                               cATCCATGAACTGACCATGCAGAGGATGATGCTCGTGCCGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATTAGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCTTGCCGTCGGCGGGGTG‑AAGTTCAACCACCGCACGCTAGAGATTCGGGAGTTAGGCGCTTCACAGTTTCGGGTTTTCGACGTTCAGAAGTAGGGTGCCGCGATccgc                                                                                                    >  2:311113/1‑247 (MQ=255)                                                                                                                                           gaccATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACg                                                                                        <  1:351158/250‑1 (MQ=255)                                                                                                                                           gaacATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGAT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTT‑CGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCt                                                                                      <  2:12493/248‑1 (MQ=255)                                                                                                                                                    gagGATGATGCTCGTGACGGTTAACGCCTCGACTCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTTTGACGCGATCGGCATAACCACCACGCTCATCGAt                                                                               >  1:501264/1‑250 (MQ=255)                                                                                                                                                     agGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATa                                                                              >  1:178546/1‑250 (MQ=255)                                                                                                                                                       gatgatGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGGG‑CAGTTCAACCGCCGCACGATAGAGATGCGGGATTTCGGCTCTCCACAGTGTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAAtt                                                                           <  1:306726/251‑1 (MQ=255)                                                                                                                                                       gatgatGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTCGCCGTT‑CAGCAGCAGCAGACTATTTTCAATCCGCACCTCGCGAAAACCGGCATCGCAGGCTTCTGCGTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGAGTCGGGATTTCGGCGCTCCACATTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCGTCGATAAtt                                                                           <  2:47968/251‑1 (MQ=255)                                                                                                                                                                 gTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCCccgccg                                                                  >  1:123724/1‑250 (MQ=255)                                                                                                                                                                 gTGACGGATAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGCTAGCGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCCCCACGCTCATCGATAATTTCAccgccg                                                                  >  1:497728/1‑250 (MQ=255)                                                                                                                                                                         tacccccTCGAATCACCAACG‑CCTGACGTTTCAGCACCAGCAGACCATTTTCAACCCCCCCCTCGCTAAAACCTACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCACCCCCCGCGAGTAAGAGATTCGGCATTTCGGCGCTCCACGATTTCTGGTTTTCGGCGTTCAGTCGTTGTGTGACCCGATCGCCATAACCACCACGCTCTTCGATAATTTCGCCGCCGAAAGacgc                                                          <  2:437436/245‑1 (MQ=255)                                                                                                                                                                              ccTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGcc                                                    >  1:427900/1‑251 (MQ=255)                                                                                                                                                                               cccgaatacgCAACGGCTTGCCGTG‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTCCAACCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCCCCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCg                                                   <  2:462957/242‑1 (MQ=255)                                                                                                                                                                               cTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCg                                                   >  2:141288/1‑251 (MQ=255)                                                                                                                                                                                  cgaaccagcaccggttggccttccagcagcaccagCCCATTTTCAATCCGCCCCTCGCGAAACCCGACATCGCGGGCTTCTGCTTCAATCAGCGTGCCGGCGGGGGTGTG‑CAGTTCAACCACCGCACGAAAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTGCGACGTTCAGACGTAGTGTGACGCGAGCGGCATAACCACCACGCTCATGGATAATTTCACCGCCGAAAGGCGCGGTGCCGCt                                                 <  2:436975/228‑1 (MQ=255)                                                                                                                                                                                   aagcAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATACGCACCTCGCGCAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CCGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCCGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCAGCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTgg                                               >  2:386522/4‑251 (MQ=255)                                                                                                                                                                                    aTCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGc                                              >  1:12034/1‑251 (MQ=255)                                                                                                                                                                                         cAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCt                                         >  1:9111/1‑251 (MQ=255)                                                                                                                                                                                          aaCGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCgg                                                                                                     <  1:385808/190‑1 (MQ=255)                                                                                                                                                                                            cgccttcccGTT‑CAGCAGCAGCAGACCATTTTCAACCCGCCCCTCGCAAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTTTG‑CAGTTCAACCCCCGCACGATAGAGATTCGGGATTTCGGCGCCCCACAGTTGCGGGTTTTCGACGTTCAGACGTGGTGTGGCGCGATCGGCATACCCACCGCGCTCATCGAGAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCg                                      <  2:547884/244‑1 (MQ=255)                                                                                                                                                                                             ggCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGCG‑CAGATCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGCTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAAGCGCGGTGCCGCTGGCGACCTGCGt                                     >  1:40748/1‑251 (MQ=255)                                                                                                                                                                                              gctggCCGTT‑CAGCAGAAGAAGCCCATTTTCAACCCGCCCCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCCCCACGCTCATCGTTAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGtt                                    <  2:418680/247‑1 (MQ=255)                                                                                                                                                                                                 ggCCGTT‑CAGCAGCAGCAGCCCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGGGCCGTCGGCGGTGTG‑CATTTCAACCCCCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGAGCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGttt                                   <  1:306971/248‑1 (MQ=255)                                                                                                                                                                                                   ccGTT‑CAGCAGCAGCAGACCATTTTCAAGCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGGGCCGTCGGCGGTGGG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCAcc                               <  1:188812/251‑1 (MQ=255)                                                                                                                                                                                                     gTT‑CAGCAGCAGCAGCCCATTTTCAATCCGCCCCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGGGCCGTCGGCGGTGTG‑CAGTTCAACCCCCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGCCGCGATCGGCATACCCCCCACGCTCATCGATATTTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCt                             <  2:137761/251‑1 (MQ=255)                                                                                                                                                                                                         cagaagcagcagACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCCCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGcc                          <  2:505876/247‑1 (MQ=255)                                                                                                                                                                                                             agcagcagACCAGTTTCAAGCCCCACCCCGCGAAACCCGACATCGCAGGCTTCGGCTTCAAGCAGCTTGCCGTCGGCGTTGTG‑CAGTTCAACCTCCGCACGATAGAGATTCGGGATTTCGGCGCTCCTCATTTTCGGGTTTTCGCCGTTCAGACGTAGTGTGCCGCGATCGGCATAACCACCACGCTCATCGATAATTCCCCCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATaa                      <  1:423810/251‑1 (MQ=255)                                                                                                                                                                                                               cagcagACCATTTTCAATCCGCCCCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAACCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGGGACGCGATCGGCATAACCCCCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAg                    <  1:359392/251‑1 (MQ=255)                                                                                                                                                                                                                  cagcccATTTTCAACCCGCACCGCGCGAAAACCGCCAGCGCAGGTTGCTGCTTCAATCAGCGGGCCGTCGGGGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACATTGTCGGGTTTTCGACGTTCAGACGTAGTGGGACGCGATCGGCATAACCACCGCGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGaaa                 <  1:305652/247‑1 (MQ=255)                                                                                                                                                                                                                  cagACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGaa                  >  2:432031/1‑250 (MQ=255)                                                                                                                                                                                                                    ggccATTTTCAATCCGCACCTCGCGAAAACCGACACCGCAGGCTTCTGCTTAAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCCCCGCACGAAAGAGATTGGGTATTGCGGCGCTCCACTGTTTCGGGTTTTCGACGTCAAGACGTAGTGTGCCGCGATCGGCAAAACCCCCACGCTCATCGAAAATTCCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACt               <  2:3095/249‑1 (MQ=255)                                                                                                                                                                                                                            tCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCCCCGCACGATAGAGATTCGGGATTTCGGCGCTCCCCAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATACCCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTAccc        <  1:11341/250‑1 (MQ=255)                                                                                                                                                                                                                            tCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCg       >  2:503454/1‑251 (MQ=255)                                                                                                                                                                                                                             cAATCCGCACCTCGCGAAAACCGACATCGAAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGCTAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCgt      >  2:74330/1‑251 (MQ=255)                                                                                                                                                                                                                             cAATCCGCACCCCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCg       <  1:101402/250‑1 (MQ=255)                                                                                                                                                                                                                               acccGCACCCCGCGAAAACCGACGTCGCTGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCCGGATAGAGATTCGGTATTCGGGCGCTCAACAGTTTCGGGTTTCCGACGTTCAGACTTAGTGTGACGCGACCGGCATAACCACCACGCCCATCGATAATTCCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCgtag    <  2:103587/249‑1 (MQ=255)                                                                                                                                                                                                                                   gCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCgtaggt  >  1:427843/1‑249 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                CCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTG‑CAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGT  >  NC_000913/365072‑365545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑