Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	343,523	G→A	A214T (GCA→ACA)	yahK →	aldehyde reductase, NADPH‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	343,523	0	G	A	93.2%	168.9 / ‑3.4	60	A214T (GCA→ACA)	yahK	aldehyde reductase, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base A (21/34);  total (25/35)
Fisher's exact test for biased strand distribution p-value = 2.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGG‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTAT  >  NC_000913/343283‑343761                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   gtcgtTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGGCTGGGACACTTGGGGGTTTAGCTGGCCCACCC‑CGTGGGGGGCC‑ATGTGGTGGGAATT‑AACACTTCTTGGGGAAAACGCGAAGCGA‑C‑aaaaagccc                                                                                                                                                                                                                                        >  2:187738/1‑246 (MQ=255)    gtTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGGCTGGGGCATATTGGGATTAAGCTGGCCCACGCCGA‑GGGGGCACCAGGTGG‑GGGAATT‑ACCACTTCTGTGGCAAAACGCGGAGCGA‑C‑AAAAGCCC‑Cggg                                                                                                                                                                                                                                    >  1:493825/1‑251 (MQ=255)           aGCGATATGTCCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGGGCAGGGATCACCCCGTAGTCGCCGCTTCGTCACTGGTAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAA‑CGGAC‑AAACGCCC‑TGGGGGCCGAt                                                                                                                                                                                                                             <  2:169929/251‑1 (MQ=255)                 atGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAgtt                                                                                                                                                                                                                       <  1:233219/251‑1 (MQ=255)                   gTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGGCTGGGGGATATGGGGGTTAAGCCGGCCCCCGC‑GATGGGGGGAC‑ATGTGGGGGCCTTT‑ACCACATCTGAGGCAAAAAGCGAAGCGC‑C‑AAAAGACC‑TGGGGGCCGATGAAggtgg                                                                                                                                                                                                                     >  1:70746/1‑247 (MQ=255)                            atgcGTCGCCCGCAGGAGCGGCTGGCGGCGGTGGGTCCTTTGTTGTGTGCAGGAATCGCCACGGATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTGTGGGACATATGGGGATTAAGCTGGCACACGC‑GATGGGGGCAC‑AGGTGGTGGCATTT‑ACCCCTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGATGTTATCTCAc                                                                                                                                                                                                             <  2:51427/247‑1 (MQ=255)                                gTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGATTAAGCATGCACACGC‑GGTGGGGGCAC‑ATGTGGTGGCATTTTACC‑CTTCTGAGGGAAAACCGGAAGCGG‑CCAAAACACC‑TGGGGGC‑GGTTAAGTTGTTAACTCACGCaa                                                                                                                                                                                                         >  2:388969/1‑250 (MQ=255)                                gTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGTATTT‑ACCACTTCTGAGGCAACACGCGAAGCGA‑C‑aaa                                                                                                                                                                                                                                              <  2:324537/214‑1 (MQ=255)                                gTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTAGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCAAATGGGGGGTAAGCTGGCCCCCGC‑GCTGGGGGCAC‑ATGTGGTGGGCTTT‑ACCACTTCTTAGGCAAAAAGCGAGACGG‑A‑AAAAACCC‑CGGGGGCCGATGGAGGTGTTTACTccaacaa                                                                                                                                                                                                         >  2:128496/1‑244 (MQ=255)                                  cACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGc                                                                                                                                                                                                      <  1:410141/251‑1 (MQ=255)                                      cGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCCTCTGGGGATTAAGGTGGCCCACGC‑GGTGGGGGCCC‑CTGTGGGGGCATTT‑AACACTTCTGGGGCAAAACGCGCAGCGA‑A‑AAAAGCCC‑TTGGGGGCGGTGAAgtttgtacatcaagcaatgccg                                                                                                                                                                                                    >  1:69252/1‑230 (MQ=255)                                               agcTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGGTTTAGCTGGCCCACGC‑GATGGGGGGAA‑ATGTGGGGGGCTTT‑ACCACTTCTTAGGCAAAAAGCGAAGCGA‑C‑AAAAACCC‑TGGGGGCCGGTGAAGTTGTTAACTCACGccaagccggatagaaggcg                                                                                                                                                                                         >  1:277766/1‑233 (MQ=255)                                                     gggcgttggcatcTTTGTTGTGTGCAGGGTACACCACGTATTCGCCGCTACGTCACTGGCATGCCGTGCCGGGTAAAAAAGTGGGCGGGGTCGGCATCGGCGGTCTTGGACATAGGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTTGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGAGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTGGTTAACTCACGCAATGACGATGAGATGGCGGCTCAt                                                                                                                                                                                   <  2:50490/239‑1 (MQ=255)                                                       gcggTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTACAAAAGCGGGCGTGGTCGGCATCGGCGGACTGGGACATATGGGGATTAAACTGGACCCCGC‑GATGGGGGGCC‑ATGTGGGGGGATTT‑ACCACTTCTGAGGCACAAAGCGCAGCGA‑C‑AAAAACCC‑TGGGGGCCGATGGAGATTTTAAATCACGCAATGCCGGTGAGATggcgggccatct                                                                                                                                                                                 >  1:92877/1‑244 (MQ=255)                                                        cggTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑Tg                                                                                                                                                                               <  1:223910/251‑1 (MQ=255)                                                           tGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAAAGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCCCTGAAGTTGTTAAATCACGCAAAGCCGATGAGATGGCGGCTCATC‑TGa                                                                                                                                                                              >  1:425035/1‑249 (MQ=255)                                                                ccTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGttt                                                                                                                                                                       <  1:35830/251‑1 (MQ=255)                                                                   ttgttgTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCg                                                                                                                                                                     <  1:463650/250‑1 (MQ=255)                                                                             cAGGGATCACCACGGATTCGCCGCTACGTCCCTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTg                                                                                                                                                          <  2:478253/251‑1 (MQ=255)                                                                              aGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATAAGGGGGTTAAGCTGGCCCAAGC‑GGTGGGGGCAC‑AAGTGGGGGCATTT‑ACCACTTCTGAGGCAAAACGCGGAGCGA‑C‑ACAAGCCCCTGGGGGC‑GGTTAAGGTGGTAACTCACGCAAAGCCGATGAGATGGCGGCCCATC‑TGCAGAGGTTCGATTTccatttg                                                                                                                                                          >  1:73093/1‑244 (MQ=255)                                                                                    caccacGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCCCTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAg                                                                                                                                                   <  1:538918/251‑1 (MQ=255)                                                                                     cccacGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCGTGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGTCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGTGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGt                                                                                                                                                  <  1:541468/250‑1 (MQ=255)                                                                                      ccacGTATTCGCCGCTGCGTCACGGGCTGGTCGGGCCGGGTAAAAAAGGGGGCGTGGTCGGCATGGGCGGTCTGGGACATAGGGGGATTAAGCTGGCCCACGC‑GATTGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACTCGAAGCTA‑C‑AAAAGCCC‑TGGGGGACGATGAAGTTGTTAACTCACGCAATGCCGAGGAGATGGCGGATCATC‑TGAAGAGTTTCGAGTTCATTGTGAATACAGTa                                                                                                                                                 <  2:564563/251‑1 (MQ=255)                                                                                      ccacGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCAGC‑TGAAGAGTTTCGATGTCATTTTGAATACAGTa                                                                                                                                                 <  1:488082/251‑1 (MQ=255)                                                                                      ccacGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCAATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTa                                                                                                                                                 <  1:564524/251‑1 (MQ=255)                                                                                                  cgcTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACAt                                                                                                                                     <  1:517368/251‑1 (MQ=255)                                                                                                   gcTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGGATATGGGGATTACGCCGGGCCACGC‑GGTGGGGGCAC‑ACGTGGTGGCGTTT‑ACCCATTTTGTGGCAAAAAGCGGAGCGA‑C‑AAAAATCC‑TGGGGGCCGCGAAAGATGTTATATCACGCCATGCCGCTGAGATGGCGGCCCCTCATGA‑GAGTTTTCATTTCATTTTGAATACAATAGCTGCGCCCCAt                                                                                                                                     >  1:554276/1‑250 (MQ=255)                                                                                                    cTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGGTTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAAAGCGAAGCGG‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATg                                                                                                                                                                                                 >  1:5633/1‑190 (MQ=255)                                                                                                    cTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATg                                                                                                                                                                                                 <  2:5633/190‑1 (MQ=255)                                                                                                         tCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGACTGGGGCATATGGGGGTTAAGCTGGGCCACGC‑GATGGGGGCAC‑ATGTGGTGGGCTTT‑ACCACTTCTTAGGCAAAAAGCGAAGCGA‑A‑AAAAAACC‑TGGGGGCCGCTGAAGTTGTTAAATCACGCAAAGCCGAGGGGAAGGGGGCGCATC‑TTAAAAGTTTCGGTTTCATTTTGAATACAATAGCTGCGCCACCTAATCTcg                                                                                                                              >  1:301569/1‑251 (MQ=255)                                                                                                           ccTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGTGACATATGGGGATTAAGCGGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATGT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCGCATC‑TGAAGAGTTTTGATTGCATTTTGAATACAGTAGCTGCGCCACATAATCTcgac                                                                                                                            <  2:270891/250‑1 (MQ=255)                                                                                                                     cgggccgggTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTacca                                                                                                                  <  1:338236/251‑1 (MQ=255)                                                                                                                            ggTAAAAAAGTGGGCGTGGTCGGGATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTGACCCCCTTGCt                                                                                                           <  2:57527/251‑1 (MQ=255)                                                                                                                                         gCGTGGGCGGCATCGGCGGTCTGGGACATATGGGGATGAAGCTGGCCCACGC‑TATGGGGGCAC‑ATGTGGTTGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGGTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATGTCATTTTGAATACAGTAGCTGCGCCACAGAATCTCGACGATTTTACCACCTTTCTGAATCGTTATGGc                                                                                              <  2:303466/251‑1 (MQ=255)                                                                                                                                                  gCATCGGCGGTCTGGGACCTATGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAACCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTTAATACAGTAGCt                                                                                                                                              >  1:393291/1‑194 (MQ=255)                                                                                                                                                  gCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCt                                                                                                                                              <  2:393291/194‑1 (MQ=255)                                                                                                                                                           gTCTGGGACATATGGGGATTAAGCTGGCCCACGC‑GCTGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCtggttggt                                                                            >  2:253128/1‑251 (MQ=255)                                                                                                                                                               gggACATATGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCTCCATGACGCTGGTTGGTg                                                                           <  1:412646/248‑1 (MQ=255)                                                                                                                                                               gggACATATGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTgc                                                                          <  1:368016/249‑1 (MQ=255)                                                                                                                                                                     tatGGGGATTACGCTGGCCAACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGCGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCg                                                                   >  2:344279/1‑250 (MQ=255)                                                                                                                                                                            aTTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGc                                                            >  2:8421/1‑250 (MQ=255)                                                                                                                                                                             ttAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCtggt                                                                                <  1:430518/229‑1 (MQ=255)                                                                                                                                                                             ttAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCtggt                                                                                >  2:430518/1‑229 (MQ=255)                                                                                                                                                                                 gCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGAGTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAAt                                                      <  1:291747/251‑1 (MQ=255)                                                                                                                                                                                 gCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGAGGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGGTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAAGCTCGACGAGTTTACCACCTTGCTGAGGCGGGATGGCACCATTACGCTGGTTGGTGCGCCTGCGACACCGCATAAAt                                                      <  1:222586/251‑1 (MQ=255)                                                                                                                                                                                   tGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGAAGCTGGTTGGTGCGCCTGCGACACCGCATAAATCg                                                    >  1:213110/1‑251 (MQ=255)                                                                                                                                                                                    ggCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGc                                                   >  1:353430/1‑251 (MQ=255)                                                                                                                                                                                            c‑gATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGtt                                           <  2:425035/251‑1 (MQ=255)                                                                                                                                                                                                  gtggCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTGTTTGGTGCGCCTGCGACACCGCATAAATCGCCTGAAGTTTTCaa                                      <  2:343622/249‑1 (MQ=255)                                                                                                                                                                                                     gCAC‑ATGTGGTGGCATTT‑ACCCCTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCGTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCt                                   <  1:222477/251‑1 (MQ=255)                                                                                                                                                                                                        c‑aTGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGAt                                <  2:64501/251‑1 (MQ=255)                                                                                                                                                                                                          aTGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCCTGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTgttc                               >  1:256107/1‑248 (MQ=255)                                                                                                                                                                                                             tggtggCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATg                            >  2:280081/1‑251 (MQ=255)                                                                                                                                                                                                              ggtggCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGa                           <  1:535820/251‑1 (MQ=255)                                                                                                                                                                                                                 ggCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGaaa                         <  1:187738/250‑1 (MQ=255)                                                                                                                                                                                                                  gCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCt                                   <  2:170072/239‑1 (MQ=255)                                                                                                                                                                                                                  gCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCt                                   >  1:170072/1‑239 (MQ=255)                                                                                                                                                                                                                    aTTT‑ACCACTTCTGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGcc                     >  1:471087/1‑251 (MQ=255)                                                                                                                                                                                                                                 tGAGGCAAAACGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCg         >  1:242023/1‑251 (MQ=255)                                                                                                                                                                                                                                        aaaCGCGAAGCGA‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTAt  <  1:527085/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   GTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGC‑GATGGGGGCAC‑ATGTGGTGGCATTT‑ACCACTTCTGAGGCAAAACGCGAAGCGG‑C‑AAAAGCCC‑TGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATC‑TGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTAT  >  NC_000913/343283‑343761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑