Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,571,280	C→T	E99K (GAG→AAG)	yjiR ←	fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,571,280	0	C	T	100.0%	149.4 / NA	48	E99K (GAG→AAG)	yjiR	fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/36);  total (12/36)

GATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTG  >  NC_000913/4571062‑4571526                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                         gatgTCATCGGCGTAGACCCCCGGGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGTACCTGATCCCACTGGGTaa                                                                                                                                                                                                                          <  2:39387/251‑1 (MQ=255)         cggcggTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCAcc                                                                                                                                                                                                                  >  1:185121/1‑251 (MQ=255)          ggcggTGACCACCGAGCCGCTGTCGAGCACCAGGGGGGCAATCTGCTCTCGCAATACTCGCTGACCGGTTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCg                                                                                                                                                                                                                 <  2:540795/251‑1 (MQ=255)          ggcggAGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTACCAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCAccc                                                                                                                                                                                                                 >  2:48075/1‑250 (MQ=255)             ggggACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCCCCACCCGGCTTAGCCCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGgc                                                                                                                                                                                                              <  1:525729/248‑1 (MQ=255)                gACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCt                                                                                                                                                                                                           >  2:206317/1‑251 (MQ=255)                   caccGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAAAACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGACACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTAGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGc                                                                                                                                                                                                         >  1:291558/1‑250 (MQ=255)                      ggggccacggTCGAGCATCAGGCGGACATTCTTCGCTCGCAATACCCGCTGACCGCCTAACAGGGCTTACCCGAGAACGGTTTGCAGATTAGGCTGCACCCCCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCg                                                                                                                                                                                                     <  2:9097/242‑1 (MQ=255)                       gAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCgg                                                                                                                                                                                                    >  1:4310/1‑251 (MQ=255)                                 cGAGAATCAGGCGGGCAACCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAGCGGTTTGCAGATTAGGCTGCACCCCCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATc                                                                                                                                                                                          <  2:107284/251‑1 (MQ=255)                                       gcAGGCGGGCATTCGGCCCTCGCAATACTCGCGGACCGGCTAACAAGTCATAACCGACACCGGTTTGCAGATTACGCTGCACCGCCCTGCTTGTCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTGCGACATCCGGTGTGCTTGTGCGTAACGGAACAATGTAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGt                                                                                                                                                                                    <  1:586020/250‑1 (MQ=255)                                           gCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCCTAACCGAGAACGGGTTGCAGATTCTGCTGCCCCACCCAGCTAAGCTCCCGCCACAGCGGTTCCAGGCTGGCCGCTTCGACATCCGGCGTGCTTTACCTACACGGAACCATGGAACTGACGCTCTGCGCCTTCAGCATATCCAGCACCTGCTACCACTGGGTAATTTCCACAGGACGCTGCACCGGACGCGTCATCGGAGGtgcct                                                                                                                                                                                >  1:293938/1‑247 (MQ=255)                                                      gctctcgcaatcccaccgaaCCGGCTAACCAGTCATAACGGAGAACGGTTTGCAGATTTTGCTGCCCCCGCCGTCTTAGCTCCCGCCACAGCGGGTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCGTATCCAGCGCCTGATCCCTCCGGGGAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTggg                                                                                                                                                                       <  2:234962/230‑1 (MQ=255)                                                          tcGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTggcggcgtgggtttac                                                                                                                                                                 >  1:420798/1‑241 (MQ=255)                                                            gCAATACTCGCTGAACGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCAAGCACCTGATCCCACTGGGTCATTTCCACCGGGCGCTGCACCGGACGCGTCATcggcggaaccagcgggctgggcgtttcg                                                                                                                                                               >  2:242214/1‑229 (MQ=255)                                                                        gACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCCCCCGGCTTAGCCCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACaaaa                                                                                                                                                  <  1:59980/251‑1 (MQ=255)                                                                          ccgtcTAACAACGCATAACGGAGACCGGTGTGCGGATTATGCTGCCCCACCCGGCTGAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATa                                                                                                                                                <  2:261273/247‑1 (MQ=255)                                                                                    aGTCATACCCGAGAACGGTTTGCAGATTATGCTGCACCCCCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAAcgc                                                                                                                                     <  1:432065/251‑1 (MQ=255)                                                                                      tCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGAACTTGCGGCGGGGGCTTT‑CGTtgtttggccaacaaaaacccacgcgc                                                                                                                                    >  2:524331/1‑228 (MQ=255)                                                                                        aTAACCGAGAACGGTTGGCAGATTAGGCTGCCCCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCt                                                                                                                                                                          <  2:453495/212‑1 (MQ=255)                                                                                          aaCCGAGAACGGTTTGCAGATTATGCCGCACCCCCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTg                                                                                                                                <  1:470885/250‑1 (MQ=255)                                                                                                gaACGTTTGGCAGATTAGGCTGCCCCCCCCGGCTTAGCCCCCGCCACAGCGGTGTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGtg                                                                                                                         <  2:97544/251‑1 (MQ=255)                                                                                                   cGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTTCGGTTTGCGCAAAAATAAACCCGA‑CGCGGCTGCgggggggtg                                                                                                                      >  1:474113/1‑244 (MQ=255)                                                                                                     gTTTGCAGATTAGGCTGCCCCCCCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAg                                                                                                                    <  2:222444/251‑1 (MQ=255)                                                                                                                      caccccCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCg                                                                                                   <  1:158608/251‑1 (MQ=255)                                                                                                                              gccTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATAc                                                                                           <  2:5529/249‑1 (MQ=255)                                                                                                                               gCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACg                                                                                          <  1:108653/251‑1 (MQ=255)                                                                                                                                 ttAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGGTTGG‑GTTTTCGTTGTGCGGCCAAAAAA‑ACCGCACGCGGCTGCGGGGGGGTGAGGTTCATCGTCTCCcgcgaccgttacgcc                                                                                        >  1:312459/1‑240 (MQ=255)                                                                                                                                          gCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACg                                                                               <  2:314775/251‑1 (MQ=255)                                                                                                                                               aGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCt                                                                          <  1:537490/251‑1 (MQ=255)                                                                                                                                                     ttCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATAc                                                                     <  1:582500/250‑1 (MQ=255)                                                                                                                                                      tCAGGCTGGGCGCTTCGACATCCGGGTTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATAGCCAGCTCCTGAGCCCACGGTGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTATTTGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCTTCTGATACGCCTGCTGCACGGTTCTGATACt                                                                    <  1:509038/250‑1 (MQ=255)                                                                                                                                                             gggCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGt                                                            <  2:546885/251‑1 (MQ=255)                                                                                                                                                                  cTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCt                                                         <  2:150533/249‑1 (MQ=255)                                                                                                                                                                    tCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGTGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCt                                                      <  1:395821/250‑1 (MQ=255)                                                                                                                                                                    tCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTcc                                                       <  1:498549/249‑1 (MQ=255)                                                                                                                                                                     cGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCGGATCCCACTGGGTAATTTCCACCGGGCGCTGCAACGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGa                                                    <  2:189702/251‑1 (MQ=255)                                                                                                                                                                                     tttGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑GTTTTCGTTGTGGGGCAAAAAAAACCCCAA‑GCGGCCGCGGGGGGGTGAGGTTCATCCGCTCCCCCGGCTGATACGCCCGCTGCCCGGGGGTGATACTTTCGCCGGGCTCCCGACTTTAGCTGCGCAccc                                    >  1:58356/1‑250 (MQ=255)                                                                                                                                                                                     tttGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCAc                                      <  1:206317/249‑1 (MQ=255)                                                                                                                                                                                      ttGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCg                                    <  1:147948/250‑1 (MQ=255)                                                                                                                                                                                             aCGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAAt                            <  1:212534/251‑1 (MQ=255)                                                                                                                                                                                                gAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGGGATGAGCTTCAGCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACGGACGCCGTGCTCCTGACt                                                  <  2:62387/226‑1 (MQ=255)                                                                                                                                                                                                     aTGGAACTGTCGCTATGCGCCTTCAGCAGACCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCTTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCCGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTcccc                    <  2:230238/251‑1 (MQ=255)                                                                                                                                                                                                       tgaacggctgCTATGCGCCTTCAGCATAGCCAGCACCTGTTCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGTCGTGCGCCTGACTGAAGCTGCGCACCGACGGCAATTTCTCCCCg                   <  2:399099/241‑1 (MQ=255)                                                                                                                                                                                                               cGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGGCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATAc          <  2:245459/251‑1 (MQ=255)                                                                                                                                                                                                                   aTGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGc       <  2:58356/250‑1 (MQ=255)                                                                                                                                                                                                                       gcCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTg  <  2:185121/251‑1 (MQ=255)                                                                                                                                                                                                                       gcCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCGGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTg  <  2:420798/251‑1 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                         GATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGG‑CTTTACGTTGTGCGACAAAATAA‑CCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTG  >  NC_000913/4571062‑4571526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑