Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	340,248	G→A	E28E (GAG→GAA)	yahI →	carbamate kinase‑like protein YahI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	340,248	0	G	A	98.4%	206.3 / NA	61	E28E (GAG→GAA)	yahI	carbamate kinase‑like protein YahI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (28/32);  minor base C (1/0);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGGCTGGCGCGTCACGGCGAGAAGAAAGCCGT  >  NC_000913/340001‑340493                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      ggATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCaggcggaagcg                                                                                                                                                                                                                                                    <  2:139674/250‑1 (MQ=255)  aaacaGCGTAGCGCATCCGTCATTTGTCACCAGTGCCGGATGCGGCATGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAATCGTAGCGCATCAGGCAGTCAGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGCCATAACAACATGAAAGAGCTTGGGGTCGTTGCCATTGGTGGCAACAACATAAGCAAAGATACCGCGAGCCAGTCTATTGAGCATCaggcggaagcgg                                                                                                                                                                                                                                                   <  2:61219/247‑1 (MQ=25)             cgcATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTc                                                                                                                                                                                                                                        <  1:82100/251‑1 (MQ=255)                  cAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCTCCCGCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGGCGATTGAGCATCAGGCGGAAGCTGTGAAAGCCGTCGCCGa                                                                                                                                                                                                                                   <  2:585210/251‑1 (MQ=255)                   aGGCATTTGTCACCATTGCCGGAATCGGCGTGAACGCCTTTTCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGTGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCAGTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGAt                                                                                                                                                                                                                                  <  2:391492/251‑1 (MQ=255)                          tGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCTGTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCTATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTg                                                                                                                                                                                                                            <  1:45041/250‑1 (MQ=255)                             cACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCATACGAGCGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGAAATAACAACATGAAAGAGCTTGTGGTCGTTGCCCTTGGTGGCAACAACATTATCAAAGATAACGCCAGCCCGTCGATTGAGCATCCGGAGGGAGAGGAGAAAGCCGTCGCCGATACGGGGCTgg                                                                                                                                                                                                                        >  2:335933/1‑251 (MQ=37)                              aCCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGa                                                                                                                                                                                                                       <  2:27803/251‑1 (MQ=255)                                      cGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTgg                                                                                                                                                                                                               >  1:566137/1‑251 (MQ=255)                                       ggATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGAGCGAGAATTGTAGGCCTGATACGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACCTGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCCGCCAGTCGATTGAGCATCAGGCGGGAGCGGTGAAAGCCGTCGCCGCAACGGGGCTGGCAATGCttgc                                                                                                                                                                                                              >  2:568455/1‑248 (MQ=38)                                                    aCGCCTTATCCGGCATACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGAc                                                                                                                                                                                                 >  1:469133/1‑251 (MQ=255)                                                     cGCCTTATCCGGCCTACGAGGGGCGCGAGAATTGTAGGCCTGCTAAGCGTAGCGCATCAGGCAGGCTGGCGTTGGTCATAACCCCATCACCCTCTTTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACa                                                                                                                                                                                                <  2:165067/251‑1 (MQ=255)                                                       ccTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACAtt                                                                                                                                                                                              <  1:256946/251‑1 (MQ=255)                                                       ccTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGGGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACAtt                                                                                                                                                                                              <  2:301963/251‑1 (MQ=255)                                                                    aCGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACg                                                                                                                                                                                 <  2:440091/251‑1 (MQ=255)                                                                     cGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACgg                                                                                                                                                                                <  1:486107/251‑1 (MQ=255)                                                                      gAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGc                                                                                                                                                                               >  1:339266/1‑251 (MQ=255)                                                                              cgAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTGGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGcc                                                                                                                                                                       <  2:322226/251‑1 (MQ=255)                                                                               gagaATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCg                                                                                                                                                                      <  1:243791/251‑1 (MQ=255)                                                                                  aaTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGc                                                                                                                                                                     >  1:351993/1‑249 (MQ=255)                                                                                        aGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCggg                                                                                                                                                             >  1:511410/1‑251 (MQ=255)                                                                                        aGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCgg                                                                                                                                                              <  2:568745/250‑1 (MQ=255)                                                                                         ggCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGc                                                                                                                                                            >  1:108056/1‑251 (MQ=255)                                                                                                 aagagTAGCGCAGCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATGATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAATCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGGCATTTTGCTGACCCACGGCAACGGGCCGCAGGTCGGGTTGGATTTa                                                                                                                                                    <  2:282152/247‑1 (MQ=255)                                                                                                      tAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCg                                                                                                                                               >  2:552678/1‑251 (MQ=255)                                                                                                      gaGCGCATCAGGCAGTCTGGCGTTTGTCCAAACCCCATCACCCTCTGTAGCGGACATAACCACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCg                                                                                                                                               <  2:583203/250‑1 (MQ=255)                                                                                                           cATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGACCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGACGCGGTGAAAGCCGGCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGCCATTGGGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCgg                                                                                                                                          >  1:541288/1‑251 (MQ=255)                                                                                                            aTCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGAttt                                                                                                                                                     >  1:7825/1‑239 (MQ=255)                                                                                                            aTCAGGCAGTCGGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGAttt                                                                                                                                                     <  2:7825/239‑1 (MQ=255)                                                                                                              cAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGgaga                                                                                                                                       <  1:172491/251‑1 (MQ=255)                                                                                                               aGGCAGTCTGGCGTTGGTCAGAACCCCATCACCCTCTGGAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGAt                                                                                                                                      <  2:412122/251‑1 (MQ=255)                                                                                                                    gTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGCCATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGCTTGccc                                                                                                                                 >  1:277911/1‑251 (MQ=255)                                                                                                                        ggCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACTGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCcaca                                                                                                                              >  1:461765/1‑250 (MQ=255)                                                                                                                             tGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGcgcg                                                                                                                        >  1:436308/1‑251 (MQ=255)                                                                                                                                 cATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCGCGGCATCGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAgg                                                                                                                    <  2:43048/251‑1 (MQ=255)                                                                                                                                        ccATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGccc                                                                                                             >  1:246605/1‑251 (MQ=255)                                                                                                                                              cccTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAAcg                                                                                                       <  2:246605/251‑1 (MQ=255)                                                                                                                                                  ctGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTAGCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAAcgcc                                                                                                     <  1:470858/249‑1 (MQ=255)                                                                                                                                                    gTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAAcgccg                                                                                                    <  1:470958/248‑1 (MQ=255)                                                                                                                                                     tAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTgg                                                                                                >  2:151407/1‑251 (MQ=255)                                                                                                                                                       gCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCg                                                                                              >  2:574179/1‑251 (MQ=255)                                                                                                                                                           aCATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACt                                                                                          >  1:397236/1‑251 (MQ=255)                                                                                                                                                                acaacaTGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACtgtgtg                                                                                     >  2:369891/1‑251 (MQ=255)                                                                                                                                                                       gAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCAGTCGCCGATCCGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGCTTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCCGGCGCACTGTGTGGCAGAt                                                                               >  2:37614/1‑250 (MQ=255)                                                                                                                                                                             gCTTGTGGTCGTTGCCATTGGGGGAAACAACATTATCAAAGATAACGCCCGCCAGACGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGACGATACGGTGCTGGAAATGCTGGCTTCCGATTACGCCATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGTACTGTGTGGCAGCTACGCAgg                                                                        >  2:297714/1‑251 (MQ=255)                                                                                                                                                                                     gtggtGCCGTTGGTGGCAATAACAGTACGAAAGATACCGCCAGCCAGTCGATTGACCACCAGGCGGAATGGGTGAAAGCCGTCGCTGATACGGTGCTGGAAATGCTTGCTTCCGATTACGACATTGTGTTGACCCACGGCAACGGGCCGCAGGTCGGGATGGATTTACGCAGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATGCGCAGGGCGGAATc                                                                <  2:541288/247‑1 (MQ=255)                                                                                                                                                                                               tggtggCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCCTCGGCTATCTg                                                       >  2:40068/1‑250 (MQ=255)                                                                                                                                                                                               tggtggCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTg                                                       >  1:115312/1‑250 (MQ=255)                                                                                                                                                                                                ggtggCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGAt                                                     <  2:108056/251‑1 (MQ=255)                                                                                                                                                                                                 gtggCAACAACATTAACAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATc                                                    <  1:572972/251‑1 (MQ=255)                                                                                                                                                                                                      aacaacATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACa                                               <  2:56219/251‑1 (MQ=255)                                                                                                                                                                                                       acaacATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCTGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAg                                              <  2:508226/247‑1 (MQ=255)                                                                                                                                                                                                           cATTATCAAAGATAACGCCCGCCAGTAGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGACGCCGCAACGTTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATATACGCCGTGCGGAGATTGAACACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACTCCGGGGGGCGTCGGCTATCTGTTcccaacggcgg                                          >  2:385439/1‑242 (MQ=255)                                                                                                                                                                                                           cATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGCAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGAAACGGGCCGCAGGTCGGACTGGATTTACGCCGTGAGGAGATTGCCAACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTTATCCAACAggagg                                          >  2:413598/1‑248 (MQ=255)                                                                                                                                                                                                           cATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGgcgc                                          >  1:42286/1‑251 (MQ=255)                                                                                                                                                                                                               aTCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGa                                       >  1:237448/1‑250 (MQ=255)                                                                                                                                                                                                                     gATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCg                                <  2:339266/251‑1 (MQ=255)                                                                                                                                                                                                                      aTAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCgg                               <  2:115312/251‑1 (MQ=255)                                                                                                                                                                                                                                ccagTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGGCTGGCGCGTc                     <  1:33012/251‑1 (MQ=255)                                                                                                                                                                                                                                    gcGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGGCTGGCGCGTCACgg                 <  2:187320/250‑1 (MQ=255)                                                                                                                                                                                                                                         tGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTTGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGGCTGGCGCGTCACGGCgag             <  2:351993/250‑1 (MQ=255)                                                                                                                                                                                                                                             cATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGGCTGGCGCGTCACGGCGagaaga         >  1:123870/1‑250 (MQ=255)                                                                                                                                                                                                                                              aTCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGGCTGGCGCGTCACGGCGAgaagaa        >  1:503378/1‑250 (MQ=255)                                                                                                                                                                                                                                                   gcggaagcggTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCCAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGGCTGGCGCGTCACGGCGAGAAGAAAGCCGt  <  1:21193/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      TGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAACTGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGGCTGGCGCGTCACGGCGAGAAGAAAGCCGT  >  NC_000913/340001‑340493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑