| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,529,039 | C→T | G402D (GGT→GAT) | sgcC ← | putative PTS enzyme IIC component SgcC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,529,039 | 0 | C | T | 98.7% | 242.7 / ‑5.7 | 79 | G402D (GGT→GAT) | sgcC | putative PTS enzyme IIC component SgcC |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (38/40); minor base A (0/1); total (38/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
GGCTTTGTCGATAACCCAGTTCATCCCCAAC‑TGCGCATCAAAACTCGGGTCGCCGGGCAATGCACGTAGA‑T‑GGCACAT‑GGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGG > NC_000913/4528792‑4529283 | ggCTTTGTCGATAACCCAGTGCATCCCCAAC‑TGCGCATCAAAACTCGGGGCGCCGGGCAATGCACGTAGA‑T‑GGCACAT‑GGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAAAA‑CAGCACGACGCTGTCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGa < 1:378355/251‑1 (MQ=255) tttGTCGATAACCCAGTTCATCCCCAAC‑TGCGCATCAAAACTCGGGTCGCCGGGCAATGCACGTAGA‑T‑GGCACAT‑GGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAAc > 1:432907/1‑250 (MQ=255) tttgtcgtaaaccaggtccttcccAACCTGCGCAGCAAATCCCGGGTGGCCGGTCAATGCACGTAGA‑T‑GGCACAT‑GGCGATGTCCGCTTTTTCCTTCCAAATAACCTCTGTCAGCCACCGCATTAAAAGA‑GTTCCTTATCCCGATAATTCAC‑TTTAACTAAAAAGCAGCAGGAAGCGGGGCACAAACCCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAAc < 1:523243/229‑1 (MQ=255) gTCGATAACCCAGTTCATCCCCAAC‑TGCGCATCAAAACTCGGGTCGCCGGGCAATGCACGTAGA‑T‑GGCACAT‑GGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACatat > 1:157096/1‑251 (MQ=255) gTCGATAACCCAGTTCATCCCCAAC‑TGCGCATCAAAACTCGGGTCGCCGGGCAATGCACGTAGA‑T‑GGCACAT‑GGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACaact > 1:209908/1‑248 (MQ=255) gTCGATAACCCAGTTCATCCCCAAC‑TGCGCATCAAAACCCGGGTCGCCGGGCAATGCACGTAGA‑T‑GGCACAT‑GGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCACCTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGAAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACatat < 1:221191/251‑1 (MQ=255) cgataacccatgcaacccccAAC‑TGCGCATCAAACCGCGGGGCGCCGGGCAAGGAACGTAGA‑T‑GGCACAT‑GGCGAGGACCGCTTTTTCCGCCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACCCCTTATTCCGATAATTCCGGTTTACGTAAGAA‑CAGCACGAGGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTg < 2:72272/235‑1 (MQ=255) agATAACCCAGTTCCTCCCCAAC‑TGCGCATCAAAACGCGGGTCTCCGGGCAATGCACTAAGA‑T‑GGCACAT‑GGCGATGCCCGCTTTTCCCGTCCCAATAACCTCTTGCAGCCACAGCATTGAAAGT‑ACTCCTTATCCCTATAATTCCCGTTTACGTAAGAA‑CACCCCGCCGCGGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAAGCGACCAATCGAACATATTg < 2:26146/250‑1 (MQ=255) aaCCCAGTTCATCCCCAAC‑TGCGCATCAAAACTCGGGTCGCCGGGCAATGCACGTAGA‑T‑GGCACAT‑GGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGccc > 1:88810/1‑250 (MQ=255) accccagtgcaccccaaacggccaaTCAAAACCCGGGTCGCCGGGCAATGCACGTAGA‑T‑GGCAAAT‑GGCGATGACCGCTTTTTCCTTCCCAATAACCTCTTACAGCCAACCCATTGAAAGT‑ACCCCTTATTCCGATAATTCCCGTTTACGTAAGAA‑CGGCACGACGCTGGCAACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCa < 1:206854/227‑1 (MQ=255) aCCCAGTTCATCCCCAAC‑TGCGCATCAAAACTCGGGTCGCCGGGCAATGCACGTAGA‑T‑GGCACAT‑GGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGCAATCGACCAATCGAACATATTGCCCAc > 1:203394/1‑251 (MQ=255) cccATTTCAGCCCCAAC‑TGCGCATCAAAACACGGGCCGCCGGGAAATGCACGTAGA‑T‑GGCACAT‑GGCGATGCCCGCTTTTTCCGCCCCAATACCCTCTTTCACCCAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCCCGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACg < 1:401515/251‑1 (MQ=255) ccAGTTCATCCCCAAC‑TGCGAATCAAAACTCGGGTCGCCGGGCAATGCACGTAGA‑T‑GGCACAT‑GGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACgg > 2:173430/1‑251 (MQ=255) cAGTTCATCCCCAAC‑TGCGCATCAAAACTCGGGTCGCCGGGCAATGCACGTAGA‑T‑GGCACAT‑GGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACgg > 2:100526/1‑250 (MQ=255) tCATCCCCAAC‑TGCGCATCAAAACTCGGGTCGCCGGGCAATGCACGTAGA‑T‑GGCACAT‑GGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAAc > 1:363477/1‑251 (MQ=255) caccccaaactccccATCAAAACTCGGGTCGCCGGGAAATGCACGTAGA‑T‑GGCACAT‑GGCGATGACCGCTTTTCCCGTCCCAATAACCTCTTTCAGCCAACTAATTGAAAGT‑ACTCCTTATCCCGATAATCCACGTTTACGTAAGAA‑CAGCACGACGCGGGCAACAATACCTACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACAGATTGCCCACGGACAACg < 2:584477/237‑1 (MQ=255) ccAAC‑TGCGCATAAAAACTCGGGTCGCCGGGAAATGCACGTAGA‑T‑GGCAAAT‑GGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACTCCTTATCCCGATAATTCACGTTTACTTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTgag < 2:423654/251‑1 (MQ=255) aaC‑TGCGAATCAAACCTCGGTGCGCCGGGCAAGTCCCGTAGA‑T‑GGCACTT‑GGCAATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACCCATTGAAAGT‑ACTCCTTATACCAATAATTCCCGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATCCCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGAt < 2:54859/251‑1 (MQ=255) gcaacAAACCTCGGGTCGCCGGGCAATGCACGTAGA‑T‑GGCACAT‑GGCGATGACCGCTTTTTCCGTCCCAATAACCGCTTTCAGCCAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTgcgc < 1:42512/247‑1 (MQ=255) aaTCAAAACCCGGGTCGCCGGGCAATGAACTTA‑A‑TAGGCAAAT‑GGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTCCAGCCAACTCATTGAAAGT‑ACTCTTTATTCCGATAATTCACGTTTCCGTAAGAA‑CAGCACGACGCGGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGATATTTGCGCa < 2:42209/250‑1 (MQ=255) aTCAAAACTCGGGTCGCCGGGAAATGCACGTAGA‑T‑GGCACAT‑GGCGATGACCGCTTTGTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACTCCTTATTCCGAAAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCAc < 1:257130/251‑1 (MQ=255) cAAAACTCGGGTCGCCGGGCAATGCACGTAGA‑T‑GGCACAT‑GGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATCCCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACt < 1:100526/250‑1 (MQ=255) aaaaCTCGGGTCGCCGGGCAATGCACGTAGA‑T‑GGCACAT‑GGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACACCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACttt > 1:10353/1‑251 (MQ=255) aacccGGGTCGCCGCTCAATGCACGTAAA‑C‑GGC‑CATTGGCGATATCGGCTTTTTCCGCCCCAAAAACCTTTTTCAGCCAACTCATTGAA‑GTGACTCCTTATGCCGATAATTCCCTTTCTCGTAAGAA‑CAGAACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAACCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACACCGCTGAGATTTGCGCACTGTCa < 2:105229/247‑1 (MQ=255) cgggggTCCCCGGGCAAGGCCCGTAGAGT‑GACA‑AT‑GGATATGACCCCTTTTCCCGTCCCAATAACCCCTTTCACCCAACTCATTGAAAGT‑CCTCCTTATACCAATAATTCACGTTTACGTAAGAA‑CAGCCCGACGCTGGCGACAATCCCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCAcc < 2:432907/248‑1 (MQ=255) cGCCGGGCAATGCACGTAGA‑T‑GGCACAT‑GGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCa > 2:38741/1‑213 (MQ=255) cGCCGGGCAATGCACGTAGA‑T‑GGCACAT‑GGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCa < 1:38741/213‑1 (MQ=255) cGGGCAATGCACGTAGA‑T‑GGCACAT‑GGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTg > 2:320888/1‑251 (MQ=255) gTAGA‑T‑GGCACAT‑GGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTg > 1:220933/1‑251 (MQ=255) gt‑GGCACAG‑GGCGATGACCGCTTTTCCCGCCCCAAAACCCTCTTTCACCCAACCCATTGAAAGT‑ACTCCTTATTCCGATAATTCCCGTTTACGTAAAAA‑CAGCACGACGCTGGCGAAAATCCCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGGTTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAgcg < 2:91744/250‑1 (MQ=255) t‑GGCGATGACCGCTTTTTCCGTCACCATAACCTCTTTCAGCCACGTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCCCGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGCGCGCCATTTc > 2:45773/1‑250 (MQ=255) ggCGATGACCGCTTTTTCCATCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAACACTGAAACCGCCTTTAAGCGCCATTTc > 1:506197/1‑249 (MQ=255) caaggACCGTTTTTCCCGCCCAAATACCCTCTTTCAGCAAACTCATGAAAAGT‑CCTCCTTATTCGAAAAATTCACGTTTACGAAAAAA‑CAGCACGACGGGGGCGACAATCCCGCCCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTg < 1:365995/247‑1 (MQ=255) gATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTg > 1:292291/1‑250 (MQ=255) gcaccccttttccccccccataaaCCCCTTTCAGCCACCGCATGGAAAGT‑CCTCCTTATCCCGAAAATTCCTGTTTACGAAAGAA‑CGGCACGACGTGGGAGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAg < 1:3725/229‑1 (MQ=255) tttcccGTCCAAATACCCTCTTTCAGCCAACTAATTAAAAGT‑ACTCCTAATCCCGATAATCCCCGTTAACGTAAAAA‑CAGAACGACCCTGGCGACAAGCCCGCCCGCCACGACACCGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTGTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGgcg < 1:552623/246‑1 (MQ=255) cGTCCAAATACCCTCTTTCAGCAACCTAATTGAAAGT‑CCTCCTTATCCCGATAATCCCCGTTTACGAAAGAC‑CAGCCCGCCGCTGGCAAAAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACGGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACt < 1:353096/251‑1 (MQ=255) gTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTg > 1:321553/1‑251 (MQ=255) ctTTCAGCCAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATcagc > 1:366691/1‑251 (MQ=255) cAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGACACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACggg > 2:19131/1‑250 (MQ=255) ccTCATTGAAATT‑ACTCCTTATTCCTATATTTCACTTTTACGTAAGAA‑CAGCACGACGCGGGCGACAAGACCGACCGCCACGACAACGCCAATAACCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTaa < 1:257082/250‑1 (MQ=255) cATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTTGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGCAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAatc > 1:257378/1‑250 (MQ=255) aTTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAatcat > 1:399386/1‑251 (MQ=255) tGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAatcatc > 1:223329/1‑250 (MQ=255) ttATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGc > 1:427592/1‑251 (MQ=255) aTTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCtt > 1:228760/1‑251 (MQ=255) ccGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATc > 1:578005/1‑251 (MQ=255) cACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAAAGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGCACTGGGTGGCGATCAGCAGGACGGTAATCATCCCGATGACCCCGCTTATCAACGTCCTg > 1:234157/1‑251 (MQ=255) gTTTACGTAAGAA‑CAGCACGCAGCTGGCGACAATACCGACCGCCACGACAAAGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGCTCAGCAGTACGGTAATCATCACGATGACCCCGCTTATCACCGTCCTGAcc > 1:200752/1‑251 (MQ=255) ttACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACACCGCCAATAATCCCAAGCGACATCAATTCAGCAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGAcc > 1:490358/1‑249 (MQ=255) aaGAA‑CAGCCCGACCCGGGCAACAACCCCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACTGTAATCATCACGATGTCCCCGCTTATCAACGTCCTGACCAGGTCTcc < 1:199737/251‑1 (MQ=255) aaGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGCTCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTcc > 2:417715/1‑251 (MQ=255) aa‑CAGCCCGACGCGGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCcgcg < 1:89621/251‑1 (MQ=255) aa‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCACAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCtt > 1:342821/1‑225 (MQ=255) gcccgccgcgggCGAAAATCCCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTgg < 1:320888/241‑1 (MQ=255) gcacgcggcgggcaccaacaccgaccgCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCAGCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTgg < 2:251929/232‑1 (MQ=255) gacgCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATa > 1:440325/1‑250 (MQ=255) cgggCGACAATCCCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCGGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGt < 1:19131/249‑1 (MQ=255) gggcgaacaTACCGCCCGCCACGACAACGCCAATAATCCCAAGCGACATAAATTCAGAAATCGACCAATCGAACATATTGCCCCCGGACACCGCGGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGTCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTa < 2:474931/243‑1 (MQ=255) gggcaaCAATACCAACCGCCACGACAACGCCAATAACCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTa < 1:498981/246‑1 (MQ=255) aCAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCACACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCAt > 1:347979/1‑251 (MQ=255) aaaacccgcccgCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATa < 2:228760/242‑1 (MQ=255) ccACGACAACGCAAATAATCCCAAGCGACACCAAGTCAGAAATCGACCAAACGAACATATTGCCCACGTACAACGCTGAGATTTGCGCCCTTTCACCGGCAAAACGGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAAc < 2:209908/251‑1 (MQ=255) ccGACAACGCCAATAATCCCAAGCGACAACAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTGGCGCACTTTCCCCGGCAAAACTGAAACCGCCTTTGCGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACgg < 1:388268/250‑1 (MQ=255) cGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGt < 1:417715/251‑1 (MQ=255) cGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGt > 1:163124/1‑251 (MQ=255) aacaccAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGAGTGGCGCACGTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGGGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTa < 2:321908/247‑1 (MQ=255) aaCGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCt > 1:11134/1‑250 (MQ=255) aCGCCAAAAACCCCAAGCGACATCAATCCAGAAACCAACCAATCGAACATATTGCCCTCGGACAACGCTGAGATTGGCGCACTTTCCCCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTAc < 2:195403/251‑1 (MQ=255) ataataCCAAGCGACATCAATTCAGTAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCCCTTTCACCGGCAAAACTGAAACCGCCTTTGAGAGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCCGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCAACCGGc > 2:396038/1‑249 (MQ=255) aGCGACATCAATTCAGAATGCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCTCTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAg < 1:268689/251‑1 (MQ=255) gACATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAgggg > 2:345238/1‑251 (MQ=255) aCATCAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTGTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGt < 1:67618/251‑1 (MQ=255) cAATTCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCCCCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAaca < 1:311663/251‑1 (MQ=255) ttCAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAacaatt > 2:207090/1‑248 (MQ=255) cAGAAATCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTttg > 1:570219/1‑251 (MQ=255) aaTCGACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCCCCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGc < 1:289101/251‑1 (MQ=255) cGACCAATCGAACATATTGCCCACGAACAACGCTGAGATTTGCGCCCTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCg < 2:11134/251‑1 (MQ=255) gACCAATCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGGAACACTTTGTTGCCCgg > 1:499002/1‑251 (MQ=255) | GGCTTTGTCGATAACCCAGTTCATCCCCAAC‑TGCGCATCAAAACTCGGGTCGCCGGGCAATGCACGTAGA‑T‑GGCACAT‑GGCGATGACCGCTTTTTCCGTCCCAATAACCTCTTTCAGCCAACTCATTGAAAGT‑ACTCCTTATTCCGATAATTCACGTTTACGTAAGAA‑CAGCACGACGCTGGCGACAATACCGACCGCCACGACAACGCCAATAATCCCAAGCGACATCAATTCAGAAATCGACCAACCGAACATATTGCCCACGGACAACGCTGAGATTTGCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGG > NC_000913/4528792‑4529283 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |