| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,524,401 | C→T | G204S (GGC→AGC) | yjhH ← | putative 2‑dehydro‑3‑deoxy‑D‑pentonate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,524,401 | 0 | C | T | 97.1% | 191.7 / ‑6.5 | 71 | G204S (GGC→AGC) | yjhH | putative 2‑dehydro‑3‑deoxy‑D‑pentonate aldolase |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (37/30); total (40/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.56e-01 | |||||||||||
TTTTGCTTCTTCAGATGCTTCAAGAATCGGTGGTAAGCAATATGTCTCTACAGGCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGATCTGCT > NC_000913/4524153‑4524646 | ttttGCTTCTTCAGATGCTTCAAGAATCGGTGGTAAGCAATATGTCTCTACAGGCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGGTTA‑CGCACCGGCGCCGccc > 2:159656/1‑250 (MQ=255) ttcttcAGATGCTTCAAGAATCGGTGGTAAGCAATATGTCTCTACAGGCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑ATATCGACCCGGCGGCGCTCagcagc > 1:2761/1‑250 (MQ=255) cAGATGCTTCAAGAATCGGTGGTAAGCAATATGTCTCTACAGGCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTa < 2:8253/251‑1 (MQ=255) cTTCAAGAATCGGTGGTAAGCAATATGTCTCTACAGGCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGCGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGATGGCGG‑GTATCGCCCCGGCGCCGCTAAGCAGCCTCGTGTTCAGc > 2:158914/1‑251 (MQ=255) tCAAGAATCGGTGGTAAGCAATATGTCTCTACAGGCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCa < 1:486681/250‑1 (MQ=255) tCAAGAATCGGTGGTAAGCAATATGTCTCTACAGGCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCa < 1:450816/250‑1 (MQ=255) tCAAGAATCGGTGGTAAGCAATATGTCTCTACAGGCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGAGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGGCGCCGCTCCGCAGCATCGTATTccgcc > 1:229463/1‑246 (MQ=255) taggCAATATGTCTCTCCAGGCAGCCCGCCACACTGCATGCTGTCTTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTGTCGCACCCTAGCCGCTCACCAACATCGTATTCCGCCAATGATCATCGtcac > 2:172069/4‑248 (MQ=255) tAAGCAATATGTCTCTACAGGCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCAGGTCAGCAGCATCGTATTCCGCAAATGATCATCGTAAc > 1:62695/1‑251 (MQ=255) aaGCAATATGTCTCTACAGGCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAAcc < 2:111289/251‑1 (MQ=255) cAATATGTCTCTACAGGCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTTTCGCACCGTCGCCGCTCAGCAGCCTTGTATTTCGCAAATGCTCATCGTAACCGcc > 1:5150/1‑250 (MQ=255) atGTCTCTACAGGCAGCCCGACACACTGCATGCTGTATTTGAGCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAAt < 2:133916/251‑1 (MQ=255) tACAGGCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACACACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑ATATCGCACCGGCGCCGCTCAGCCGCATCGTCTTCAGCAAATGATCCTCGTAACCGCAGAATACCgca > 2:236186/1‑248 (MQ=255) aCAGGCAGTCCGACACTCTGCATGCTGTAATTGATCAGTGATACAAACTGTGTTTCGAGGGAGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCATCGGTCGCCAGATCGCCTTCCCGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCTGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGaa < 1:25568/249‑1 (MQ=255) aCAGGCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCCTCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGaaa > 1:200252/1‑250 (MQ=255) aCAGGCAGCCCGACAAACTGCATGCTGTATTTGATCAGTGAGACACACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGGAATCAGCAAATGATCATCGTAACCGCAGaaaaacggaa > 2:345770/1‑242 (MQ=255) aGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCCGCAGCATCGTATTCAGCCAATGATCATACTAACCCCAGAATACCGACAacg‑accg > 2:386019/1‑249 (MQ=255) gCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCACAGCCGCATCGTATTACGCAAATGATCATCGTAAACGCAGAATACCGAAAaag‑acgcg > 1:34455/1‑249 (MQ=255) gCCCGACACACTGCATGCTGTATTTGATCAGGGAGACAAACGGTGTTTCGAGGGCGTAAAGAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACggg < 2:99865/251‑1 (MQ=255) gACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGGAAACGCAGAATACCGaaaaagaacggcct > 1:381126/1‑240 (MQ=255) cacTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGa < 1:460483/251‑1 (MQ=255) gTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑Tgtgt < 1:358465/251‑1 (MQ=255) aTTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGtt < 1:281806/250‑1 (MQ=255) gtGATCAGGTAGACAAACGGTGTTGCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTGTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGTGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGGTAGCGCTGGCGT‑TTATCGCCCCGTCGCCGCTCATCAGCATCGTATTCAGCAAATGATCATCGTAACCGCATAATACCGAAAACG‑ACGGGCGTACCGCCTTAAC‑TGTGTTGa < 2:46618/249‑1 (MQ=255) aTCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCCTCGTATTTAGCAAAAGATCATCGTAACCCCAGAATAACGAAAACGCACGG‑CCTACCGACTTAAC‑TATGTTTatca > 2:508231/1‑250 (MQ=255) cGAGGGCGTAAATAGCGGGCAGTTGTAGTAGGTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCGCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTg < 2:507291/251‑1 (MQ=255) gAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGt < 1:347982/251‑1 (MQ=255) gggCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCg < 1:499930/251‑1 (MQ=255) cGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGGCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTGTCGCCACGGCGCCGCTCCTCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGCAAACA‑ACCGGAGTACCGACTTAACATG‑GTTTATCATCGTTCGCCAGTGACCCACGCAGTCgcat > 1:76392/1‑248 (MQ=255) aaaTAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGgtg > 1:242888/1‑251 (MQ=255) aaTAGCGGGCGGTTGTAGTAGTTTTTTAGTCAGCGGCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGGAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGCCTTAAC‑TGTGTTGATCATCGTACGCAAGTCACCAACGCTGTCG‑ATGgtgt < 2:134679/251‑1 (MQ=255) aaTAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGGAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCCAGTGACCAACGCTTTCG‑ATggggt > 1:467338/1‑248 (MQ=255) tAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCCCCGTCGCCCCTCCGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCCAAAACG‑ACGGGCGTAACGACATAAC‑TGGGTTGATCATCGTACGCCAGTGGCCAACCCTGTCG‑CTggggatt > 2:107140/1‑246 (MQ=255) gggCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCCCCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTTACCGCAGAATACCGCAAACG‑ACAGGCGTACCGACTTAAC‑TGTGTTGGTCATCGTACGCAAGTGACCAACGCTGGCG‑ATGGGGTCTTTgt > 2:500115/1‑250 (MQ=255) ggCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGGTCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGAGTCTTTGAt > 1:341647/1‑251 (MQ=255) gCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TTTGTTTATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGGGTCTTTGATg > 1:128980/1‑251 (MQ=255) aTTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCCCCGTCGCCGCTCAGCAGCATCGTATTCCGCACATGATCATCGTAACCGCCGAATAACGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCCACGCTGTCG‑CTGGTGTCTTTGATGCCCACGCTTTTCTCGtttt > 1:211050/1‑251 (MQ=255) ttCAGCGGCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCCCTCCGCAGCATCGTATTCCGCCAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGAGAACAAAGCTGTCG‑ATGGTGTCTTTTTTGCCAACGACATTTTCCttttt > 2:381941/1‑250 (MQ=255) cAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑ATATCGCCCCGTCGCCGCTCAGCAGCATCGTATTCCGCAAATGATCCTCGTAACCGCAGAATACCGCAAACG‑ACGGGCGTACCGACTTTAC‑TGTGTTTATCATCGTTCGCCAGTGACCAACCCGGTCG‑ATGGTTTCTTTGATGCCCACCATATTCTCGTTTTGc > 1:1498/1‑250 (MQ=255) gCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGGGCAACGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCCTCGTATTCAGCAAATGATCATCGTAAACGCAGAATACCGGAAACG‑ACGGGCGTCACGACTTACC‑TGTGTTGATCCTCTTACGCCAGTGACCCACGCTGTCG‑GTGGTGTCTTTTATGCCAACGATATTCTCGTTTTGCAGAGcc > 2:197215/1‑249 (MQ=255) aGCGGTCGCCAGATCGCCTGCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGGCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTGGATGCCAACGATATGCTCGTTTTGCAGAGCCAGAc < 2:211050/251‑1 (MQ=255) aGCGGTCGCCAGATAGACTTAACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCACAGGTAGCGCTGGCGG‑ATATCGCACCGGCGGCTCTCAACAGCATCGTAATCAGCAAATGATCATCGTAACCGCAGAAAACCGAAAACG‑AAGGGCGGTCCGACTTAAC‑CGTGTTGATCATAGTACGAAAGTGAACAACGCTGTCG‑ATGGGGTCTTTGATTCCAACGATATTATAGTTTTGCaaggcccac > 2:404494/1‑242 (MQ=255) gCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGAcg < 1:236186/251‑1 (MQ=255) gCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCGCGTTTTGCAGAGCCAGAcg < 2:251872/251‑1 (MQ=255) ggTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGAcgcg < 1:517153/251‑1 (MQ=255) ggTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGAcgcg > 1:427862/1‑251 (MQ=255) ggTCGACAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTCTTCAGCAAATGATCATCGGAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGGCTTTGAAGCCAAAGCTATTCTCGTTTTGCAGAGCCAGAcgcg > 2:234997/1‑251 (MQ=255) ccAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTGGATGCCAACGATATGCTCGTTTTGCAGAGCCAGACGCGTCACg < 2:203251/251‑1 (MQ=255) ccAGATCGCCTGCACGGCAGGCGCGGTAGAGGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TGATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCAGCGTACGCAAGTGGCCAACGCGGTCG‑ATGGTGTCTGTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACg < 2:226826/251‑1 (MQ=255) gATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAAACGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGACGAGCCCGAAGCGTCACGGtt > 1:46640/1‑251 (MQ=255) gCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGAGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCg < 2:341647/251‑1 (MQ=255) gcgcGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTg < 1:255991/251‑1 (MQ=255) cgcgGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCCCCGTCGCCGCTCCGCAGCATAGTATTCAGCCAATGATCATCGTAACCGCCGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGCTACTCGTACCCAAGTGACCAACGCTGTCGGATGG‑GTCTTTTATGCCAACGATATTCTCGTTTTGCAGGGCCAGACGCGTCCAGGTTTCCCGGGTTAAGTCCTgc > 2:272168/1‑250 (MQ=255) cgGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGAcc < 1:107140/251‑1 (MQ=255) aGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATTGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGt < 2:290601/250‑1 (MQ=255) cGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCCCAGCCGCAACGTATTCAGCAAATGATCATCGTAACCGCAGAATAACGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTTATCATCGTACGCCAGTGACCCACCCTGTCG‑ATGGGGGCTTTGATGCCAACGATATTTTCCTTTTTCAGAGCCCCAAGCGTTCCGGGTTCCCGGGTTTAGTCCCGACCCgctcggttcgg > 2:232067/1‑240 (MQ=255) gagaGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTAACGCCCCGGCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGGCTTAAA‑TGTGTTGATCATCGGACGGAAGGGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAAAGATCTTCTCGTTTTGCAGAGCCAGACGCCTCACGGTTTTCGGGGGTAAGCCCTGACCCggccagtttggaa > 2:9679/1‑238 (MQ=255) ccGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCTCCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCTACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCATATTCGGATAGTTGTa < 2:2761/250‑1 (MQ=255) cAAAGTTAGCGCTGGCGG‑TTAGCGCACCGTCGTCGCTCAGCAGCATCGTATTCAGCAAATTATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCGCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATTCGGAAAGTTGTACAGGATc < 1:336332/251‑1 (MQ=255) aaGTTAGCGCTGGCGG‑TTAGCGCACCGTCGTCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATTCGGAAAGTTGTACAGGATCAc < 2:496584/251‑1 (MQ=255) aGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCCTCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGCTATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATTCGGAAAGTTGTACAGGATCAcc > 1:269968/1‑251 (MQ=255) gTTAGCGCTGGCGG‑TTAGCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATTCGGAAAGTTGTACAGGATCACCg < 1:545273/251‑1 (MQ=255) aGCGCTGGCGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATTCGGAAAGTTGTACAGGATCACCGGTa < 1:345770/251‑1 (MQ=255) ggCGG‑TTATCGCACCGTCGCCGCTCATCAGCATCGTATTCAGCAACTGATCCTCGTAACCGCAGAATACCGAAAACG‑ACGGGAGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATTCGGAAAGTTGTACAGGATCACCGGTAGGGTg > 1:530758/1‑250 (MQ=255) cGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATTCGGAAAGTTGTACAGGATCACCGGTAGGGTGACg > 1:331281/1‑251 (MQ=255) cGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATTCGGAAAGTTGTACAGGATCACCGGTAGGGTGACg > 1:332469/1‑251 (MQ=255) cGG‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATTCGGAAAGTTGTACAGGATCACCGGTAGGGTGACg > 1:457663/1‑251 (MQ=255) gg‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCCTCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATTCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGc > 1:289599/1‑251 (MQ=255) gg‑TTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATTCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGc > 1:270286/1‑251 (MQ=255) aCCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATTCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCg > 2:194815/1‑250 (MQ=255) gTCGCCGATCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTCAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCAAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATTCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTCCGGGCGATct > 2:381724/1‑251 (MQ=255) cgccgcTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGGCACGGTTTCCGGGGTTAAGTTCTGACCCGTCAGATTCGGAAAGTTGTACAGGATCACCGGTAGGGTGTCGCTACGGGCGATctgc < 2:49535/251‑1 (MQ=255) gccgctCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATTCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGATctgct > 1:317395/1‑251 (MQ=255) gccgctCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATTCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGATctgct < 1:386019/251‑1 (MQ=255) | TTTTGCTTCTTCAGATGCTTCAAGAATCGGTGGTAAGCAATATGTCTCTACAGGCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGG‑TTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACG‑ACGGGCGTACCGACTTAAC‑TGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCG‑ATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGACGCTACGGGCGATCTGCT > NC_000913/4524153‑4524646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |