BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,482,901	C→T	V313M (GTG→ATG)	valS ←	valine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,482,901	0	C	T	99.0%	354.7 / NA	102	V313M (GTG→ATG)	valS	valine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (44/57); minor base G (0/1); total (44/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 102 total aligned reads displayed.

CAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACACCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGA‑CATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCT  >  NC_000913/4482655‑4483139

cAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTgg                                                                                                                                                                                                                                             <  1:322868/251‑1 (MQ=255)

  cccgccgGTTTCGCCAGGACATCGGCACGCACGTACCACTGGGCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGgc                                                                                                                                                                                                                                           <  2:312301/250‑1 (MQ=255)

     gccgGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGATTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCt                                                                                                                                                                                                                                        <  1:246163/251‑1 (MQ=255)

       cgGTTTCGCCAGGACATCGGCCCGCACGTCCCACTGGTCGGTCAGCATTGTGTCGATAACTTCGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAAGTTCTTCCAGCAGGCCAAGCGCGTCAACTGCTGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGTAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGTCGCttt                                                                                                                                                                                                                                      <  2:414375/251‑1 (MQ=255)

            tCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACgg                                                                                                                                                                                                                                 >  1:165359/1‑251 (MQ=255)

            tCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACg                                                                                                                                                                                                                                  <  1:298521/250‑1 (MQ=255)

            tCGCCAGGACATCGGCACGCACGTACCACTGGGCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACg                                                                                                                                                                                                                                  <  2:118769/250‑1 (MQ=255)

              gCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGat                                                                                                                                                                                                                               >  1:126125/1‑251 (MQ=255)

                 aGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATc                                                                                                                                                                                                                            >  1:81750/1‑251 (MQ=255)

                   gACATCGGCACGCCCGTACCACTGGTCGGTCAGCATTGGTTCGATCACTACGCCGCCACGGTCGCCGTACGGAACGGTAAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGCGCGCTTTCACGGATATCGc                                                                                                                                                                                                                          >  2:79530/1‑251 (MQ=255)

                   gACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCCCGGATATCGc                                                                                                                                                                                                                          >  1:526295/1‑251 (MQ=255)

                    aCATCCGCAAGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCCACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGACCTCTGCAGGGATTTCGCTGGAATAAACGTCAGCTTCGTTACCTTTGGTATCGCACATCTGGGCGCTTTCACGGATATCGcc                                                                                                                                                                                                                         >  2:241502/1‑251 (MQ=255)

                            acgcacgTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAgg                                                                                                                                                                                                                 >  1:288373/1‑251 (MQ=255)

                            acgcacgTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAgg                                                                                                                                                                                                                 <  1:181040/251‑1 (MQ=255)

                             cgcacgTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGt                                                                                                                                                                                                                >  1:47297/1‑251 (MQ=255)

                                    aCCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGAt                                                                                                                                                                                                          <  2:159101/250‑1 (MQ=255)

                                         tGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTg                                                                                                                                                                                                      >  2:402306/1‑249 (MQ=255)

                                         tGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTg                                                                                                                                                                                                      <  1:402306/249‑1 (MQ=255)

                                         gggtcggtcAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGaa                                                                                                                                                                                                    <  2:16959/250‑2 (MQ=255)

                                              ggtcAGCATTGGTTCGCTAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCg                                                                                                                                                                                               >  2:412224/1‑251 (MQ=255)

                                               gtcAGCACGGGTTCGATAACTGCGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCAACgg                                                                                                                                                                                              <  2:147509/251‑1 (MQ=255)

                                                gcagcagTGGTTCGATAACTACGCCGCCACGTTCGCCGTAAGGAACGGTCAGGGCGTGCTGTGTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTTCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGTGAGGTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGc                                                                                                                                                                                             <  1:341780/250‑1 (MQ=255)

                                                 cAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGc                                                                                                                                                                                             <  1:555829/250‑1 (MQ=255)

                                                 cAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCa                                                                                                                                                                                            <  1:304049/251‑1 (MQ=255)

                                                     aTTGGTTCGATCAATACGACGCCACGGTCGCCGTCAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATAGCCGTCAAAGGTCAGGAAGTTGATCATCGGCAGGGc                                                                                                                                                                                        >  2:337052/1‑251 (MQ=255)

                                                     aTTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGTGATTTCGCTGGAATAAACGTCAGATGCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGc                                                                                                                                                                                        <  1:491729/251‑1 (MQ=255)

                                                     aTTGGATCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGCACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGc                                                                                                                                                                                        >  2:393518/1‑251 (MQ=255)

                                                       gggTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTGCCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGTTGTTGATCTTCGGCAGGGCGt                                                                                                                                                                                      <  2:271759/250‑1 (MQ=255)

                                                           tCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGAc                                                                                                                                                                                   <  2:145224/250‑1 (MQ=255)

                                                           tCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCGTTACGTGCAGCAAAACGCTCCAGGTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGAc                                                                                                                                                                                   <  2:288373/250‑1 (MQ=255)

                                                              aTAACTATGCCGCAACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATATCTTCCAGCAGGCCAAGCGCGACAACTGCGGCAACGACTGCTTTACGTGCAGCAACAGGCTCCAGTTTCTGGAACTCTGCAGGGTTTTCGCTGGAATATACGTCAGATTCGTTACCTTTGGTATCTCACATCTGGGCGCTTTCTCAGATATCGACGTCAAAGGTCAGGATGTTGATTATCGGCAGGCCGTGACGttt                                                                                                                                                                               >  1:417270/1‑251 (MQ=255)

                                                                aaCTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTAc                                                                                                                                                                             >  2:348697/1‑251 (MQ=255)

                                                                    aCGCCGCCACGGTCGCCTGAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCa                                                                                                                                                                          <  2:329997/250‑1 (MQ=255)

                                                                      gccgccACGGTCGCCGTAAGGAACGGTTAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACCGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTGCTGGAACTCTGTAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGTGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACtt                                                                                                                                                                       <  1:335306/251‑1 (MQ=255)

                                                                        cgccACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCg                                                                                                                                                                                                                        <  1:76106/200‑1 (MQ=255)

                                                                        cgccACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCg                                                                                                                                                                                                                        >  2:76106/1‑200 (MQ=255)

                                                                          ccACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGc                                                                                                                                                                                             <  2:157633/225‑1 (MQ=255)

                                                                          ccACGGTCGCCGTAAGGAACGGCCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGc                                                                                                                                                                                             >  1:157633/1‑225 (MQ=255)

                                                                               gTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGt                                                                                                                                                              <  1:241502/251‑1 (MQ=255)

                                                                                 cGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGtt                                                                                                                                                             >  2:295044/1‑250 (MQ=255)

                                                                                    cGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCGGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGGTTACCCACTTCATAGTCGTTAAAg                                                                                                                                                         <  2:562967/251‑1 (MQ=255)

                                                                                           aaCGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTgcg                                                                                                                                                  >  2:287988/1‑251 (MQ=255)

                                                                                            aCGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTgcgc                                                                                                                                                 <  1:455253/251‑1 (MQ=255)

                                                                                              ggTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCTCTTCATAGTCGGTAAAGTCGTGCGCCg                                                                                                                                               <  1:448385/251‑1 (MQ=255)

                                                                                                 cAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACTCTCCAGTTTCTGGAACGCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCATAGGGCAGGATGTTg                                                                                                                                                                                                      <  2:498348/193‑1 (MQ=255)

                                                                                                 cAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTg                                                                                                                                                                                                      >  1:498348/1‑193 (MQ=255)

                                                                                                      cGTGCGGTTTAAGTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGGTACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGAtt                                                                                                                                       <  2:505963/251‑1 (MQ=255)

                                                                                                       gTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCACCGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGAttt                                                                                                                                      >  2:78888/1‑251 (MQ=255)

                                                                                                         gCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGCCTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATAGCACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAAGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGTTTTTc                                                                                                                                    >  1:25810/1‑251 (MQ=255)

                                                                                                                aaTTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAg                                                                                                                              <  2:470287/250‑1 (MQ=255)

                                                                                                                 aTTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGcc                                                                                                                            <  1:412224/251‑1 (MQ=255)

                                                                                                                        ccAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGGGCGCCGGAGTGATTTGCACGCAGCCCGTGcc                                                                                                                      <  1:483197/250‑1 (MQ=255)

                                                                                                                                  aaGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGt                                                                                                           >  2:19407/1‑251 (MQ=255)

                                                                                                                                        gTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGat                                                                                                                                                                                                    <  2:16362/156‑1 (MQ=255)

                                                                                                                                        gTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGat                                                                                                                                                                                                    >  1:16362/1‑156 (MQ=255)

                                                                                                                                           aaCTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTTATCATCGGCAGGGCTTGACGTTTACCCTCTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGtt                                                                                                  <  1:321595/251‑1 (MQ=255)

                                                                                                                                            aCTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTtc                                                                                                 <  1:370535/251‑1 (MQ=255)

                                                                                                                                               gCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTtcgtc                                                                                              <  2:477083/251‑1 (MQ=255)

                                                                                                                                                   cAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGcc                                                                                           >  1:258407/1‑250 (MQ=255)

                                                                                                                                                    aaCGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCCTAGTCGTTAAAGCCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCa                                                                                          >  2:583471/1‑250 (MQ=255)

                                                                                                                                                     aCGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAAc                                                                                        <  1:437285/251‑1 (MQ=255)

                                                                                                                                                          tGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCg                                                                                   >  1:485919/1‑251 (MQ=255)

                                                                                                                                                             tttACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTAGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTAACCACTTCATAGTCGTTAAAGGCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGACTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGa                                                                                 >  1:294609/1‑250 (MQ=255)

                                                                                                                                                                aCGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATac                                                                             <  1:348697/251‑1 (MQ=255)

                                                                                                                                                                 cGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCCTGTCGGCGTGGTCGTCGCCAACGGTCGGAATacc                                                                            >  2:469856/1‑250 (MQ=255)

                                                                                                                                                                        caAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTa                                                                     <  2:166672/251‑1 (MQ=255)

                                                                                                                                                                           aaCGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAAcc                                                                  <  1:321214/251‑1 (MQ=255)

                                                                                                                                                                                 ccAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCgg                                                             <  2:113318/250‑1 (MQ=255)

                                                                                                                                                                                 ccAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCg                                                              <  1:393518/249‑1 (MQ=255)

                                                                                                                                                                                 ccAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGc                                                            <  1:19407/251‑1 (MQ=255)

                                                                                                                                                                                 ccAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGGCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGAGGTTTATCATCGGCAGGGCGTGACTTTTACCCTCTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACTGTTAACCAGCgg                                                             <  1:573558/250‑1 (MQ=255)

                                                                                                                                                                                 ccAGTTTCGGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGGATCGAACAGCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCgg                                                             <  2:258407/250‑1 (MQ=255)

                                                                                                                                                                                  cAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCa                                                           <  2:165359/251‑1 (MQ=255)

                                                                                                                                                                                    gTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGa                                                         <  2:157031/251‑1 (MQ=255)

                                                                                                                                                                                          ggAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCAGCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTGCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATg                                                      <  2:66333/248‑1 (MQ=255)

                                                                                                                                                                                              ttctGCCGGGATGTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCGGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGACTACGACGGTAAACCAGCGGCAGAAAGA‑CATAtt                                              >  2:355256/2‑251 (MQ=255)

                                                                                                                                                                                               tctGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAAACAGAGGCAGACTGA‑CATAttt                                             >  1:406485/1‑251 (MQ=255)

                                                                                                                                                                                                  gCAGGGATTTCGCTGGCCTAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTAAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGACTACGACGGTTAACCCGCGGCAGCATGA‑CATATTTGcc                                          >  2:558807/1‑251 (MQ=255)

                                                                                                                                                                                                     ggtattgcGCTGGAATACACGTCAGATGCGTTACCTTGGGTAGAGAACATCTGGGCGCTGTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTCTACCCACTGCATAGTCGTTAAAGACGTGCGCCGGAGTGATTTTCACGCAGGGGGTGTCTTTTTCCATGTCGGGGGGTTTGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCGGAATGA‑CATTTGTGCCAAt                                       <  2:25810/244‑1 (MQ=255)

                                                                                                                                                                                                     gggATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCAc                                                                                                                                  <  2:375962/161‑1 (MQ=255)

                                                                                                                                                                                                     gggATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCAc                                                                                                                                  >  1:375962/1‑161 (MQ=255)

                                                                                                                                                                                                      ggATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCgg                                                             <  1:367729/229‑1 (MQ=255)

                                                                                                                                                                                                      ggATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCgg                                                             >  2:367729/1‑229 (MQ=255)

                                                                                                                                                                                                      ggATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGAGCGGAATACGACGGTTAACCAGCGGCAGAATGA‑CATATTTGCCAATc                                      <  1:71320/251‑1 (MQ=255)

                                                                                                                                                                                                       gATTTAGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTACAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGA‑CATATTTGCCAATct                                     >  1:315030/1‑250 (MQ=255)

                                                                                                                                                                                                          ttCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGA‑CATATTTGCCAATc                                      >  1:26554/1‑247 (MQ=255)

                                                                                                                                                                                                           tCGCTGGAATAAACGTCAGATTCGGTACCTTTGGTAGCGAACATCTGGGCGCTTTCACGGATGTCGCCGTCAAAGGTCAGTATGTTGATCGTCGGCAGGGCGGGACGTGTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTGTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCATAATGA‑CATATTTGCCAATCAGAt                                  <  2:237618/250‑1 (MQ=255)

                                                                                                                                                                                                                gtaaTAAACGTCAGATTCGTTACCTGTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCAGCGGCAGGGCGGGACGTTTACCCACTTCATAGTCGTTAAAGTCTTGCGCCGGAGTGATTTTCACGCAGCCGGGGCCGTGTTCCATGGCGGCTGGTTCGTCGCCAACGATCGGAAGAGGACGGTTAACCAGCGGCAGAA‑GAGCATGTTTGCCTATCAGATCttt                              <  2:68051/247‑1 (MQ=255)

                                                                                                                                                                                                                gtaaTAAACGTCAGATTCGTTACCTCTGGGATCGAACATCTGGGCGCTGTCACGGATATCGCCGTCAAATGACAGGAGTTTGAGCATCGGCAGGGCGTGACGTTTTCCCACTTCATAGTCGTAAAAGTCGTGCGCCGGAGTGAGTTTCACGCGGCCGGTTCTTTGTTCCATGTCGGCGTACTCGTCGCCAACGGTCGGAAGTCGACGGTTAACCAGCGGCAGAATGA‑Cataatcgcgaaacggttgtgtgt                            <  2:236714/249‑21 (MQ=255)

                                                                                                                                                                                                                ggAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCACAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCCGCGGCAGAATGA‑CATATTTGCCAATCAGTTCTTTGt                            >  2:501380/1‑251 (MQ=255)

                                                                                                                                                                                                                 gAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGA‑CATATTTGCCAATCAGATCTTTGt                            >  1:185468/1‑250 (MQ=255)

                                                                                                                                                                                                                  aaTAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGA‑CATATTTGCCAATCAGATCTTTGTa                           >  2:437068/1‑250 (MQ=255)

                                                                                                                                                                                                                        cGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGA‑CATATTTGCCAATCAGATCTTTGTAACGCggg                    >  2:173958/1‑250 (MQ=255)

                                                                                                                                                                                                                         gTCAGATTCGTTACCTTTGGTATCGGACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGA‑CATATTTGCCCATCAGATCTTTGTAACGCGGAt                   >  2:286211/1‑251 (MQ=255)

                                                                                                                                                                                                                         gTCAGATTCGTTACCCTTGGTATCGAACATCTGGGCGCTTTCACGGATAGCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGGGTTCGTCGCCAACGATCGGAAGACGGCGGTTAACCAGCGGCAGAATGA‑CATATCTGCCAGTCAGATCGTTGTATCGCGGAt                   <  2:47266/251‑1 (MQ=255)

                                                                                                                                                                                                                          tCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGA‑CATATTTGCCAATCAGATCTTTGTAACGCGGATc                  >  1:499362/1‑251 (MQ=255)

                                                                                                                                                                                                                            aGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGACGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGA‑CATATTTGCCAATCAGATCTTTGTAACGCGGATCtt                >  1:521011/1‑251 (MQ=255)

                                                                                                                                                                                                                            aGATTCGGTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTTATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGACGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCTGAATACGACGGTTAACCAGCGGCAGAATGA‑CATATTTGCCAATCAGATCTTTGTAACGCGGATCtt                <  1:371745/251‑1 (MQ=255)

                                                                                                                                                                                                                                   ttACCATTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGGTAACCAGCGGCAGAATGA‑CCTATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGtt         >  2:531864/1‑251 (MQ=255)

                                                                                                                                                                                                                                           ggTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCCTCGGCAGGGCGTGCCGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGA‑CATATTTGCCAATCAGATCTTTGTAAAGCGGATCTTCCGGGTTACCGGCt  >  2:546008/1‑250 (MQ=255)

CAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACACCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGA‑CATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCT  >  NC_000913/4482655‑4483139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`