Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,435,750	C→T	G272G (GGG→GGA)	cpdB ←	2',3'‑cyclic‑nucleotide 2'‑phosphodiesterase/3'‑nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,435,750	0	C	T	100.0%	166.8 / NA	55	G272G (GGG→GGA)	cpdB	2',3'‑cyclic‑nucleotide 2'‑phosphodiesterase/3'‑nucleotidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/33);  total (22/33)

GCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAAT  >  NC_000913/4435503‑4435994                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     gCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTTCCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATGAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACGCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCt                                                                                                                                                                                                                                                   <  1:517985/251‑1 (MQ=255) gCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCt                                                                                                                                                                                                                                                   <  1:421658/251‑1 (MQ=255)  cTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCtt                                                                                                                                                                                                                                                  >  1:63366/1‑251 (MQ=255)    tGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCTATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCtt                                                                                                                                                                                                                                                  <  2:154931/249‑1 (MQ=255)    tGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTc                                                                                                                                                                                                                                                 <  1:145685/250‑1 (MQ=255)    tGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTc                                                                                                                                                                                                                                                 <  1:545127/250‑1 (MQ=255)        gACGAACTGGCGTGTGGCATCGTGATCGGCTGTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAAGCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCGTCGata                                                                                                                                                                                                                                             <  2:243163/250‑1 (MQ=255)         aCGAACTGGCGTGTGGCAGCGGGATCGGCTTTGAGTGTTTCTCCCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACTCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATc                                                                                                                                                                                                                                           <  1:533717/251‑1 (MQ=255)          ccatatGGCGTGTGGCCTTGTTATCGGATTTGAGTGTTTCTGCCAGCTTGCTGTCTGCAGCCGCGACGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTGGCCTGCGTCACCTGCCATTTACCGCTGTCATTTCTGAGTTGTAAGTCGACCACTCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCTGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGAAATCa                                                                                                                                                                                                                                          <  2:138486/246‑1 (MQ=255)             aCTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGc                                                                                                                                                                                                                                        >  1:357818/1‑250 (MQ=255)               tGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCaa                                                                                                                                                                                                                                      >  1:14397/1‑250 (MQ=255)               tGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCaa                                                                                                                                                                                                                                      >  2:498589/1‑250 (MQ=255)                 gCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAAt                                                                                                                                                                                                                                   <  1:90941/251‑1 (MQ=255)                   gtgtGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTCGCGCTGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCt                                                                                                                                                                                                                                 >  1:277517/1‑251 (MQ=255)                   gtgtGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTATGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCCGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCt                                                                                                                                                                                                                                 >  2:250934/1‑251 (MQ=255)                          aTCGTGATCGGCCTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTGCGATATCAGCAAAATCTTTACCTg                                                                                                                                                                                                                          <  2:272135/251‑1 (MQ=255)                            cGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTggg                                                                                                                                                                                                                        <  1:577464/251‑1 (MQ=255)                             gTGATCGGCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTggg                                                                                                                                                                                                                        >  1:87651/1‑250 (MQ=255)                                          aGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGTATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGAGCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGc                                                                                                                                                                                                          <  2:469959/251‑1 (MQ=255)                                             gtTTCTACCAGCTTGCTGTCGTCCGCCGCGTGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTGGTAAGTCGACCACACCAAGATGATCGTCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATg                                                                                                                                                                                                       <  1:564426/251‑1 (MQ=255)                                                     cagctggctagcTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCTTAAATCGGTCGTTCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTCCTGAGTTGTAATTCGACCACACCAAGATGAGCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATTGCCAAACa                                                                                                                                                                                               <  2:478458/240‑1 (MQ=255)                                                               tCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGtt                                                                                                                                                                                     >  2:462490/1‑251 (MQ=255)                                                                  tCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTa                                                                                                                                                                                    <  1:275892/249‑1 (MQ=255)                                                                   ccgcctCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGAGATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCAGGGCCAAACATAATGGCGTTAACg                                                                                                                                                                                 <  2:525104/251‑1 (MQ=255)                                                                              gATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAACGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCCTGGCCAAACATAATGACGTTAACGACCGGAATTTc                                                                                                                                                                      >  1:16546/1‑251 (MQ=255)                                                                                 tttttATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACt                                                                                                                                                                   >  1:425900/1‑251 (MQ=255)                                                                                    ttattaGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTg                                                                                                                                                                  >  1:210287/1‑249 (MQ=255)                                                                                     tattaGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGACACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAATATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAgg                                                                                                                                                               >  1:568882/1‑251 (MQ=255)                                                                                        taGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGta                                                                                                                                                             <  1:350419/250‑1 (MQ=255)                                                                                             aTGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTGCGATATCAGCAAAATCTTTACCGGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAAc                                                                                                                                                       <  1:355924/251‑1 (MQ=255)                                                                                                      aTCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTc                                                                                                                                              <  2:357818/251‑1 (MQ=255)                                                                                                            cGTGTTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAACCTGAGTTTTCCGCCAt                                                                                                                                        <  2:417625/251‑1 (MQ=255)                                                                                                                  tCCGCTTTCGCCTGCGTCACCTGCCATGTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCGGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTTAGTTTTCCGCCATCACtt                                                                                                                                   <  1:462754/250‑1 (MQ=255)                                                                                                                           gCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATc                                                                                                                         >  2:532679/1‑251 (MQ=255)                                                                                                                           gCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATc                                                                                                                         >  2:5998/1‑251 (MQ=255)                                                                                                                             cTGCGTCACCTGCCATTTACCGCAGTCATTACTGAGTTGTAAGGCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTGACCTGGGAAAACGGCGTGAGCATGGCCAAACATAAGGGCGt                                                                                                                                                                                      <  2:96243/188‑1 (MQ=255)                                                                                                                             cTGCGTAACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATACTGGCGt                                                                                                                                                                                      >  1:96243/1‑188 (MQ=255)                                                                                                                                        gCCATTTACCGCTGTCATTACTGAGGTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGcc                                                                                                              <  1:366921/249‑1 (MQ=255)                                                                                                                                                              gAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATAGCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGAGCGGCAGATAGCCCGGAATGTGCCAGAACGacaaca                                                                                       <  2:583312/250‑1 (MQ=255)                                                                                                                                                                  tGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACAt                                                                                   <  1:101041/250‑1 (MQ=255)                                                                                                                                                                                    aGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACCCAATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACCTCGGCACCTTTCTCGCGCAt                                                                >  2:468604/1‑251 (MQ=255)                                                                                                                                                                                        gatCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTc                                                             >  1:90206/1‑250 (MQ=255)                                                                                                                                                                                         atCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTc                                                             >  2:258197/1‑249 (MQ=255)                                                                                                                                                                                                cacaTGCCTGGCCGTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCTCATTTCAGGCACGTa                                                    <  2:122010/251‑1 (MQ=255)                                                                                                                                                                                                     gCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCACGTATTTgc                                               >  1:120826/1‑251 (MQ=255)                                                                                                                                                                                                               aCCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCGTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGTCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCATGCACGTATGTGCGCACGGTTTc                                     <  1:177873/251‑1 (MQ=255)                                                                                                                                                                                                                ccgccgGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTGCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCg                                    <  2:291843/251‑1 (MQ=255)                                                                                                                                                                                                                      gAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCAGTTCAGGCACGTATTTGCGCACGGTTTCGGTAata                              <  1:231286/251‑1 (MQ=255)                                                                                                                                                                                                                           ccATTCAGCGTGCCTTTGGCGATAGCAGCTCCTTAGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGGCAACAACATCGGCACCTTTCTCGCTCAATTCAGGCACGTAGTTGGGCACGGTTTCGGTAATATCAtt                         <  2:58576/251‑1 (MQ=255)                                                                                                                                                                                                                            cATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGAGCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTc                        <  2:63366/251‑1 (MQ=255)                                                                                                                                                                                                                               tCAGCGTGCATTTGGCGTTATCAGCTCCTTAGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGCGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGAATCGGCAGATAGCCCGGAATGAGCCAGAACGACAACAACATCGGCACCTTTCTCGAGAATTTCAGGCACGTATTTGAGCACGGTTTCGGTAATATCATTCAcc                     >  2:209633/1‑251 (MQ=255)                                                                                                                                                                                                                                       ccTTTGGCGATATCAGCTCCTTCGATATCAGCAAAAGCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGAGCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCTCGCATTTCAGGCAAGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACtt              <  1:164197/250‑1 (MQ=255)                                                                                                                                                                                                                                          ttGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACt               <  1:194112/246‑1 (MQ=255)                                                                                                                                                                                                                                          ttGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACt               >  2:194112/1‑246 (MQ=255)                                                                                                                                                                                                                                                  atCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAAt  >  1:208771/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     GCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCTGTCTTCCGCCGCGAGGGATTTTTTATTAGCGATGTCGTAAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCCAGAACGACAACAACATCGGCACCTTTCTCGCGCATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAAT  >  NC_000913/4435503‑4435994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑