Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,414,625 C→T R117R (CGC→CGT)  aidB → putative acyl‑CoA dehydrogenase AidB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,414,6250CT98.6% 223.8 / NA 72R117R (CGC→CGT) aidBputative acyl‑CoA dehydrogenase AidB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (38/33);  minor base G (1/0);  total (39/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACACCACCC  >  NC_000913/4414383‑4414869
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    
ggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCgtgcggcg                                                                                                                                                                                                                                                <  2:165458/249‑1 (MQ=255)
    gggATAGCGATTTTCTAGCCAGTAGTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGttt                                                                                                                                                                                                                                           <  1:87304/250‑1 (MQ=255)
    gggATAGCGATGTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACGGGGGCGGCTTGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCGCCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCGGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGtttt                                                                                                                                                                                                                                          <  1:525085/251‑1 (MQ=255)
        aaGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGt                                                                                                                                                                                                                                      <  2:438134/250‑1 (MQ=255)
        aaGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCGGGACGATGTTCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGGGGCTCGTGCGGCGCGTTTTATGt                                                                                                                                                                                                                                      <  2:340229/250‑1 (MQ=255)
                      ccAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACCTGCGCAGGtt                                                                                                                                                                                                                        >  2:454591/1‑251 (MQ=255)
                            ttGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGACTTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGAACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGc                                                                                                                                                                                                                   >  1:355309/1‑250 (MQ=255)
                             tGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGGGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGc                                                                                                                                                                                                                   >  1:57364/1‑249 (MQ=255)
                                     agTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGGGGCAGGGTCGttt                                                                                                                                                                                                         >  2:455569/1‑250 (MQ=255)
                                        tAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCACGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGAAGGGTCGTTTTGt                                                                                                                                                                                                      >  1:567978/1‑251 (MQ=255)
                                         aGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGt                                                                                                                                                                                                      <  1:358905/250‑1 (MQ=255)
                                              cGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGGGGCAGGGTCGTTATGTCCGAt                                                                                                                                                                                                 >  1:307177/1‑250 (MQ=255)
                                                gCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAAc                                                                                                                                                                                              >  2:5992/1‑251 (MQ=255)
                                                          ttGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGAGCTACGAAGCGCAAGGACGACGTCTGGACGCTGTGCGTTTTCACCCCGCCTCGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACCTGCGCAGGGTTAGGCAGGGTCGGTATGGCCGCTAAACATGCCCttt                                                                                                                                                                                    >  2:567908/1‑251 (MQ=255)
                                                                  gggCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCCCCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCcgccac                                                                                                                                                                            <  1:392001/251‑1 (MQ=255)
                                                                   ggcggcTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTAGTTATGTCCGATAACCATGACCTTTGCcgccacg                                                                                                                                                                           >  1:90041/1‑251 (MQ=255)
                                                                       gcTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCCCCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGGGGCGCGTGCGGCGCGTTGTATGTTACATGCGCAGGTTTAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCAt                                                                                                                                                                       <  1:271019/251‑1 (MQ=255)
                                                                        cTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGTTAACCATGACCTTTGCCGCCACGCCAtt                                                                                                                                                                      >  2:174610/1‑251 (MQ=255)
                                                                                 gtgAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGTCCTTTGCCGCCACGCCATTGTTGTTACa                                                                                                                                                             <  2:320178/251‑1 (MQ=255)
                                                                                  ggAATCCGCCTGAATTATTGCGCTCCGATGCGTAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTGTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAg                                                                                                                                                            <  2:441382/250‑1 (MQ=255)
                                                                                    aaTCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGGCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGGTGTTACCGAt                                                                                                                                                          >  2:323907/1‑251 (MQ=255)
                                                                                        cGCCTGAATTATTGCGCTCCGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGtt                                                                                                                                                       >  2:520365/1‑250 (MQ=255)
                                                                                                  gttGCGCTACGATGCGCAAGGACGCCGACTGGACGATGTGCGTTTGCACCCCGCCTGGGACCGGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACTCTCGCTCCGGCGCATTGGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTGGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCg                                                                                                                                             <  2:136520/249‑1 (MQ=255)
                                                                                                          cctaTGCGCAAGGACTCCGTCGGTACGAGGTGCGTTTTCACCCCGCTTGGCACCTGCTGATGCAGTCTCTATGTACCAACCGGGTGCACAATCTGGCCTGGGAAGAAGACGCTCGCGCCGGCGCTTTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCTTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGAc                                                                                                                                    <  2:439945/248‑1 (MQ=255)
                                                                                                          aCGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAgg                                                                                                                                      <  1:200051/249‑1 (MQ=255)
                                                                                                           cgtggcgcAAGGACGCCGTCTGGACGAGGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAAGCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTGGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACt                                                                                                                                   <  2:467146/247‑1 (MQ=255)
                                                                                                           cGATGCGCAAGGACGCCGTCTGGCCGATGTGAGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCAGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGCTAACCATGACCTTTGCCGCCACGCCATTGTTGTTACCGATGTTACCCGCGCCGTTTAAGGACt                                                                                                                                   >  2:211039/1‑251 (MQ=255)
                                                                                                            gATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTg                                                                                                                                  >  2:290807/1‑251 (MQ=255)
                                                                                                            gATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGGCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTg                                                                                                                                  >  1:75460/1‑251 (MQ=255)
                                                                                                                        cGCCGTCGGGACGTTGGGGGTGTTCACCCCGCCTGGCCCCTGCTGATGAAGGCGCTATGTACCAATCGGGGGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTGGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGCCCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCt                                                                                                                      <  2:309084/251‑1 (MQ=255)
                                                                                                                              cTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTTTTg                                                                                                                  >  2:444142/1‑249 (MQ=255)
                                                                                                                                ggACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGc                                                                                                               <  2:167560/250‑1 (MQ=255)
                                                                                                                                    gATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCg                                                                                                          >  1:419194/1‑251 (MQ=255)
                                                                                                                                         gCGTTTTCCCCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACg                                                                                                     <  1:425492/251‑1 (MQ=255)
                                                                                                                                          cGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCCTGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGCGCGATCGCTACgc                                                                                                    >  2:555814/1‑250 (MQ=255)
                                                                                                                                                      gCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATa                                                                                                                                                                                                <  2:165169/147‑1 (MQ=255)
                                                                                                                                                      gCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATa                                                                                                                                                                                                >  1:165169/1‑147 (MQ=255)
                                                                                                                                                      gCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTAtt                                                                                        >  1:31439/1‑251 (MQ=255)
                                                                                                                                                          ggCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGcc                                                                                     >  1:120711/1‑250 (MQ=255)
                                                                                                                                                             cccTGCTGATGCAGGCGCTATGTACCAATCGGGGGCACAATCTGGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTGGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTAGGTCCGATAACCATGCCCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCAc                                                                                             <  2:297437/238‑1 (MQ=255)
                                                                                                                                                             aCCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCCCGCCGCTATTGAGCGATCGCTACGATTCTCAc                                                                                             >  1:297437/1‑239 (MQ=255)
                                                                                                                                                              ccTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTg                                                                                <  2:10776/251‑1 (MQ=255)
                                                                                                                                                                      tGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCaaaa                                                                         >  1:371453/1‑250 (MQ=255)
                                                                                                                                                                             gcTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCCAAAACGCGGtt                                                                 >  1:69251/1‑251 (MQ=255)
                                                                                                                                                                               tATGTACCAATAGGGAGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGAGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCCTTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCCAAAACGCGGTttg                                                               >  2:306150/1‑251 (MQ=255)
                                                                                                                                                                                  gTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTttgtt                                                             <  2:163933/250‑1 (MQ=255)
                                                                                                                                                                                     ccAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGAtt                                                         <  1:455569/251‑1 (MQ=255)
                                                                                                                                                                                      cAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTg                                                        <  1:174610/251‑1 (MQ=255)
                                                                                                                                                                                      cAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGAGGGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTCTGTCCGATAACCATGACATTTGCCGCCCCGCCCTTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGCCCACGCCGCTCTTGAGCGCTCGCTACGCTTTTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTg                                                        >  1:521479/1‑251 (MQ=255)
                                                                                                                                                                                        aTCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCCGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCCGGTGGGCAAAAACGCGGTTTGTTGATTGGc                                                      >  2:496965/1‑251 (MQ=255)
                                                                                                                                                                                            ggTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATgg                                                  <  1:290807/251‑1 (MQ=255)
                                                                                                                                                                                              tGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATggg                                                 >  2:268246/1‑250 (MQ=255)
                                                                                                                                                                                               gCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGaa                                               <  1:315490/251‑1 (MQ=255)
                                                                                                                                                                                                      cTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACgg                                         >  1:17378/1‑250 (MQ=255)
                                                                                                                                                                                                         gCCTGGGAAGAAGACTCTCGCTCGTGCGCTTTTGTGGCGGGTGCGGCGCTTTTTATGTTACATGCGCAGTTTGAGGCAGGGTCGTTAGGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGGCCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGaaaa                                     <  1:292017/251‑1 (MQ=255)
                                                                                                                                                                                                              ggAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCCCTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGGTTGGCATGGGAATGACGGAAAAGCAgg                                >  2:421567/1‑251 (MQ=255)
                                                                                                                                                                                                              ggAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCCCAGGTTGAGGCAGGGTCGTTATGTCCGATAACAATGACCTTTGCCGCCACGCCCTTGTTGTTACAGATGTTACCCGCCCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCCGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGaaacacagg                                >  2:276824/1‑245 (MQ=255)
                                                                                                                                                                                                                aagaagACGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGc                              <  1:426248/251‑1 (MQ=255)
                                                                                                                                                                                                                 agaagaCGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCg                             >  1:432556/1‑251 (MQ=255)
                                                                                                                                                                                                                  gaagaCGCTCGCTCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCCCCCCGCTATTGAGCGATCGCTACGATTCTCACTTCTTGCCAGGGGGGCAAAAAAGAGGTTTGTTGATTTGCATGGGAATGACGGAAAAGCAGGGCgg                            >  2:494312/1‑251 (MQ=255)
                                                                                                                                                                                                                  gaagaCGCTCGCTCAGGCGCATTTGTGGCCCGTCCGGCGCGTTTTATGTTGCATGCGCAGGTTGAGGCAGGGTAGTTCTGTCAGATCACCATGACCTTTGCCGCCACGCCATTGTTGTTACAAATTTTACCCGCCCCGGTTCAGGACTGAACCACGCCGATAATGAGCGATCGCTACGATTCTCACTTATTGCCGGGTGGGCAAAAACGAGGTTTGTTGATTGGCATGGGAATGCCGGGAACGCAGGGCgg                            >  2:75887/1‑251 (MQ=255)
                                                                                                                                                                                                                   aagtcgctcgctCCGGCGCATTTGTGGCGCGTGCCGCGCGATTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACCCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTCTTGGGCGATCGCTACGATTCTCACTTATTTCCAGGTGGGCCAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGt                           >  2:146170/5‑251 (MQ=255)
                                                                                                                                                                                                                   aagaCGCTCGCTCCGTCGCTTTTGTGGCGCGTGGTGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGTTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCATGACTGGACCACGCCGCTATTGAGCGATCTCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGt                           <  1:497733/251‑1 (MQ=255)
                                                                                                                                                                                                                       cgctcgctCCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGGAAAGCAGGGCGGTTCCg                       >  2:148929/1‑251 (MQ=255)
                                                                                                                                                                                                                        gctcgctcCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGa                      <  1:520365/251‑1 (MQ=255)
                                                                                                                                                                                                                          tcgctcCGGCGCATTTGTTGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGGCGGTATGTTCGATAACCATGACCTTTGCCGCCACGCCACTGTTGTTACTGGTGTTACCCGCGCCGTTTCAGGACAGGACAACGCCGCTACTGAGCGAGCGCTACGATTATCACTTATTGCCAGGTGTGCAAAAAGCCGGTTTGGTGATTGGCCTGGGAATGACGGAAAAGCAGGGCGGTTCCGATg                    >  2:219153/1‑251 (MQ=255)
                                                                                                                                                                                                                           cgctcCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGAt                     <  1:434228/249‑1 (MQ=255)
                                                                                                                                                                                                                           cgctcCGGCGCATTTGTGGCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGGCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCCTGGGAATGACGGAAAAGCAGGGCGGGTCCGATGt                   >  2:417901/1‑251 (MQ=255)
                                                                                                                                                                                                                               ccGGCGCATTTGGGGCGCGTGCGGCGCGTTTTATGTTACAGGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTGTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATg               <  1:311905/251‑1 (MQ=255)
                                                                                                                                                                                                                                gggggcaggtttgGCGCGTGCGGCGTGTTTTATGTTACATGCGCAGGTGGAGGCAGGGTTGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGTGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGa              <  2:130464/240‑1 (MQ=255)
                                                                                                                                                                                                                                         tgtgGCGCGTGCGGCGCGTTTTTTGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATGGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAAcacc      <  1:306150/250‑1 (MQ=255)
                                                                                                                                                                                                                                            ggCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACACCAccc  >  1:444097/1‑251 (MQ=255)
                                                                                                                                                                                                                                            ggCGCGTGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACACCAccc  <  1:188403/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    
GGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACACCACCC  >  NC_000913/4414383‑4414869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑