Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	338,656	G→A	C111Y (TGC→TAC)	yahG →	DUF1116 domain‑containing protein YahG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	338,656	0	G	A	100.0%	235.4 / NA	69	C111Y (TGC→TAC)	yahG	DUF1116 domain‑containing protein YahG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/36);  total (33/36)

TAGAAGTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTGCGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGCAACATCGCTTATACCAACATGAGCGAGCAGATGGCGAAGATTTTGCGT  >  NC_000913/338410‑338900                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      tAGAAGTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTacggc                                                                                                                                                                                                                                                   >  2:326997/1‑251 (MQ=255)      gTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATACCATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGAt                                                                                                                                                                                                                                              <  1:485163/251‑1 (MQ=255)             accTCGACTGGACGCCGCCGGGGCAGGGCAAGAGGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGgc                                                                                                                                                                                                                                       <  2:180579/249‑1 (MQ=255)             aaCTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGgc                                                                                                                                                                                                                                       <  2:6889/251‑1 (MQ=255)                 cGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCGGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCgg                                                                                                                                                                                                                                   <  1:383533/251‑1 (MQ=255)                  gacggtaCGCCGCCGGGTCAGGCCAATAGGGAGGGGGAGGAGGCCATGGATAACATTGCCGATTCGCCGCGGGCGGACAAAAGCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCTCCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGt                                                                                                                                                                                                                                  <  2:481581/245‑1 (MQ=255)                  gACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATAACCTGGGAAAAACTGTACGGCGCGATGAAAGGCGCGGt                                                                                                                                                                                                                                  >  1:464302/1‑251 (MQ=255)                   aggggACGCCGCCGGGGCAGGGCAAGAGGCAGGTGGGGCAGGCGCGGGATAACATTTCGGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCTTGACGGCGAAAACCATTCTTCACGCCGGGCCGTCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTc                                                                                                                                                                                                                                 <  1:557475/248‑1 (MQ=255)                         cgccgcCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCgg                                                                                                                                                                                                                            >  1:1755/1‑250 (MQ=255)                          gccgccGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCggcg                                                                                                                                                                                                                          >  2:421536/1‑249 (MQ=255)                            cgccgGGGCAGGGCAATATGCAGGGGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCCGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGACAGGCGCGGTCCCCGGAgcg                                                                                                                                                                                                                        >  1:323713/1‑251 (MQ=255)                                 gggcagggcaATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATGCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGgt                                                                                                                                                                                                                   <  2:267889/251‑1 (MQ=255)                                         caATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGa                                                                                                                                                                                                             >  1:267337/1‑249 (MQ=255)                                                                     aaCATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAggcggc                                                                                                                                                                               >  1:328576/1‑251 (MQ=255)                                                                        gtggCCGAGTCGCCGCTGGCGAACAAAACCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGa                                                                                                                                                                            <  2:399292/248‑1 (MQ=255)                                                                           gCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAAc                                                                                                                                                                          <  1:43098/250‑1 (MQ=255)                                                                                    ccgcTGGCGGACAAACTCGCCGCCGCTAACCAGCAGGCGCTGGCGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTc                                                                                                                                                                >  1:169717/1‑251 (MQ=255)                                                                                         ggCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCgggg                                                                                                                                                           >  1:131703/1‑251 (MQ=255)                                                                                             gACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGAt                                                                                                                                                       <  2:131703/251‑1 (MQ=255)                                                                                              aCAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCgggg                                                                                                                                                           >  1:226081/1‑246 (MQ=255)                                                                                                  aaTCGACGCCGCTAACCAGCAGGCGATGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGCCGGCGAAAACCATTCTTCACGCCGGGACGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGCAAGATCTCGACGAGGCGGCTGTACTGGCGGCTTCCGGGGGGATCACC‑t                                                                                                                                                 >  2:292119/1‑251 (MQ=255)                                                                                                     cgccgccgcTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑Ttct                                                                                                                                              <  1:60535/251‑1 (MQ=255)                                                                                                         gccgcTAACCAGCAGGCGCTGGAGCGTATTATCCAGACGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCAAC‑TTCTCGcc                                                                                                                                          >  1:235456/1‑251 (MQ=255)                                                                                                              tAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGAGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGGGATCACC‑TTCTCGCCGTGTc                                                                                                                                     >  2:260852/1‑251 (MQ=255)                                                                                                               aaCCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCAGATTGGTTTTGATCAGGCGATTAACGTGGGGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCa                                                                                                                                    <  1:260852/251‑1 (MQ=255)                                                                                                               aaCCAGCAAGCGCTGGAGCGTATTGTCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCAGGGCCGCCGATCCCCTGGGAAAAAATGTACGGAGCGATGACAGCCGCGGTCACCGGAGCGCTGGCGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGATTCCGGGGAGATCACT‑TTTCCGCCGTGTc                                                                                                                                     >  2:291814/1‑250 (MQ=255)                                                                                                                   agcagGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAg                                                                                                                                <  1:421536/251‑1 (MQ=255)                                                                                                                    gcagGCGCTTGAGCGTACTATCCAGTCGCAGCCGGTGCTGATTGGTGTTGAGCAGGCGATTAACGTGGTGCCTGGCATGACGTCTAAAACCATTCTTCCCGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCTATGAAAGGCGCTGTCACCGTAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTTATCTGGCGGCTTCCGGTGAGATCACC‑TCCTCGCCGTGTCACGAGc                                                                                                                               <  1:189207/251‑1 (MQ=255)                                                                                                                      agGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCAc                                                                                                                             <  1:92885/251‑1 (MQ=255)                                                                                                                        gcgcTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAACGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACgg                                                                                                                           >  2:251035/1‑250 (MQ=255)                                                                                                                              gAGCGTATTATCCGGTCGCATCCGGAGCTGATTGGTTTTGATCAGGCGATTACCGTGATGCCGGGCATGACGGCGAAAACAATTCTTCACGCCGGGCCGCCGCTCACCTGGGACAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGGGCGCTGGAGTTCGAAGGACTGGCGAACGCTCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGA‑CACCCTTCTCGCCGTGTCCCGGGCACGACTGCgg                                                                                                                     >  2:282378/1‑250 (MQ=255)                                                                                                                                    attatCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATc                                                                                                               >  1:128160/1‑251 (MQ=255)                                                                                                                                         ccAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATg                                                                                                           <  1:251035/250‑1 (MQ=255)                                                                                                                                                    cGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCCCCGGAGCGCTGGTGTTCGAAGGCCTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTAcc                                                                                               >  1:518647/1‑251 (MQ=255)                                                                                                                                                     ggTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCt                                                                                              >  1:43141/1‑251 (MQ=255)                                                                                                                                                               ggTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGAt                                                                                    <  1:198651/251‑1 (MQ=255)                                                                                                                                                               ggTTTTGAGCGGGCGGTTAACGTGGTGCCGGTCATGACGGCGAAAACCTTGCTTCACGCCGGGCCGCCGATCACCTGGTAAAAAAGGTACGGCGCGAGGAAAGGCTCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTTCGTGGGATCGATTGCGGGTGTTACCTCGGCCTCGAt                                                                                    <  2:464302/251‑1 (MQ=255)                                                                                                                                                                     gATCAGGCGATTAACGTGTTGCCGGGCATGACGGCGAAAACCATTCTTCTCGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGAGCGATGGCGGGTGTTACCTCGGCCTCGATGTTTa                                                                               <  1:542141/250‑1 (MQ=255)                                                                                                                                                                       tCAGGAGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCTGATCACCTGGGAAAAAATGTACGGCGCGTTGAAAGGCGCGGGCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGCACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGGGTAACCTCCGCCTCGATGTTTATGc                                                                            >  2:477928/1‑251 (MQ=255)                                                                                                                                                                        cAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTc                                                                                       >  1:375173/1‑239 (MQ=255)                                                                                                                                                                        cAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTc                                                                                       <  2:375173/239‑1 (MQ=255)                                                                                                                                                                             gATTAACGTGGTGACGGGCATGACGGCGAAAACCATACTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCg                                                                      <  2:328576/251‑1 (MQ=255)                                                                                                                                                                             gATTAACGTAGTGCCGGGCCTGACGGCGAAAACCACTCTTCACCCAGGGCCGCCGATCAACTGGGAAAACATGAACGGCGCGACGAAAGGCGCGGTCCCCGGACCGCTGGTGATCGCGGGACTGGCGAAAGATCTCGACGAGGCGGCTGAAATGGCGGCCTCCGGGGAGATCACC‑TTCTCGCCGTGTCCCGAGCACGCCTGCGTGGGACTGATGGCGGGGGGTAACTCGGCCACGCCGTTTATgagcctcg                                                                      >  2:565908/1‑244 (MQ=255)                                                                                                                                                                                tAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCCCATCGTa                                                                    >  2:218569/1‑250 (MQ=255)                                                                                                                                                                                tAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATAGATGGCGGGTGTTACCTCTGCCTCAATGTTTATGCACCTCgca                                                                    >  1:301544/1‑248 (MQ=255)                                                                                                                                                                                  aCGTGGGGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCTCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCGCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTaaaa                                                                 <  2:495728/251‑1 (MQ=255)                                                                                                                                                                                      ggtgCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAaaaac                                                              <  1:265644/250‑1 (MQ=255)                                                                                                                                                                                         gCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAaaaacaaa                                                           <  1:326997/250‑1 (MQ=255)                                                                                                                                                                                            gggCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCt                                                       <  1:88092/251‑1 (MQ=255)                                                                                                                                                                                              gCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAggcg                                                                                                                                                                                 >  1:450067/1‑128 (MQ=255)                                                                                                                                                                                              gCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAggcg                                                                                                                                                                                 <  2:450067/128‑1 (MQ=255)                                                                                                                                                                                                aTGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACgg                                                   >  1:93198/1‑251 (MQ=255)                                                                                                                                                                                                 ggACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGc                                                  <  1:437292/250‑1 (MQ=255)                                                                                                                                                                                                   aCGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCCCGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACggaa                                                 >  1:280860/1‑248 (MQ=255)                                                                                                                                                                                                     ggCGAAAACCATGCTGCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGCAACa                                              <  2:482658/251‑1 (MQ=255)                                                                                                                                                                                                       cGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGCAACATc                                            <  1:1990/251‑1 (MQ=255)                                                                                                                                                                                                        gAAAACCATGCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGCAACATCg                                           <  2:435522/251‑1 (MQ=255)                                                                                                                                                                                                          aaaCCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGCAACATCGCt                                         <  1:46033/251‑1 (MQ=255)                                                                                                                                                                                                              cATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGCAACATCGCTtata                                     <  1:157982/251‑1 (MQ=255)                                                                                                                                                                                                               aTTCTTCACGCCGGGTCGCCGCTCACCTGGGAAACAATGTAAGGCGCGATGCAAGGCGCGGTCCCCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTAGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCTCC‑TTCTCGCCGGGTCACGAGCACGGCTGCGTGGGGTCGATGGAGGGGGTTTCCCCGGCCTCGATGGTTATGCACATCGTTAAAAAAAAAAACTACTGCAACATCGCCTATAc                                    >  2:248079/1‑251 (MQ=255)                                                                                                                                                                                                                         ccGGGCCGCCGATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGCAACATCGCTTATACCAACATgagc                          <  2:267337/251‑1 (MQ=255)                                                                                                                                                                                                                              ccgccgATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGCAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGAAACATCGCTTATACCAACATgagcgagc                      >  1:389040/1‑250 (MQ=255)                                                                                                                                                                                                                              ccgccgATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACCGCAACCTCGCTTATACCAACATgagcgagc                      >  2:160129/1‑250 (MQ=255)                                                                                                                                                                                                                                 ccgATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGCAACATCGCTTATACCAACATGAGCGAGCAGAt                  <  2:226081/251‑1 (MQ=255)                                                                                                                                                                                                                                   gATCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATTGCGGGGGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGCAACCTCGCTTATACCAACATGAGCGAGCAGGTgg                >  2:29462/1‑251 (MQ=255)                                                                                                                                                                                                                                     tCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGCaa                                                >  2:435490/1‑217 (MQ=255)                                                                                                                                                                                                                                     tCACCTGGGAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCGACGGCaa                                                <  1:435490/217‑1 (MQ=255)                                                                                                                                                                                                                                             gAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGCAACATCGCTTATACCAACATGAGCGAGCAGATGGCGAAGAtttt      <  2:128160/251‑1 (MQ=255)                                                                                                                                                                                                                                             gAAAAAATGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGCGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGAAACATAGCTTATACCAACATAAGCGAGCAGATGGCGAAGAtttt      >  1:373252/1‑251 (MQ=255)                                                                                                                                                                                                                                                  aaTGTACGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGCAACATCGCTTATACCAACATGAGCGAGCAGATGGCGAAGATTTTGCGt  >  1:382818/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      TAGAAGTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTGCGGCGCGATGAAAGGCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACC‑TTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGCAACATCGCTTATACCAACATGAGCGAGCAGATGGCGAAGATTTTGCGT  >  NC_000913/338410‑338900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑