Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,401,607	C→T	T312T (ACC→ACT)	hflX →	ribosome rescue factor HflX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,401,607	0	C	T	99.0%	307.3 / NA	95	T312T (ACC→ACT)	hflX	ribosome rescue factor HflX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (46/48);  minor base G (0/1);  total (46/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

ACCCTCGACCCGACGTTGCGGCGTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGT  >  NC_000913/4401362‑4401848                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   aCCCTCGACCCGACGTTGCGGCGTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTctgct                                                                                                                                                                                                                                               >  1:194052/1‑251 (MQ=255)       gACCCGACGTTGCGGCGTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGtgat                                                                                                                                                                                                                                         >  1:119982/1‑251 (MQ=255)       gACCCGACGTTGCGGCGTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGtgat                                                                                                                                                                                                                                         >  2:183048/1‑251 (MQ=255)             gcgtggcggcgTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACaa                                                                                                                                                                                                                                   <  1:393732/246‑1 (MQ=255)             aCGTTGCGGCGTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACaa                                                                                                                                                                                                                                   <  2:172696/251‑1 (MQ=255)              cGTTGCGGCGTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGGGACTA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTATTCTGGTGATGAACa                                                                                                                                                                                                                                    >  1:430450/1‑249 (MQ=255)               gTTGCGGCGTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGAGACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGGACCAGACAAAATCGCAGCGGTGAATA‑CGGGTCTTGTAGAGGCTGACGCTCCAGAGATCCCAACTCAGCTGGGGTTGAACAAgc                                                                                                                                                                                                                                 >  2:37576/1‑250 (MQ=255)                 tGCGGCGTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACa                                                                                                                                                                                                                                    >  1:554245/1‑246 (MQ=255)                  gcggcgTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACa                                                                                                                                                                                                                                    >  1:297818/1‑245 (MQ=255)                    ggcgTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGat                                                                                                                                                                                                                            <  1:333313/251‑1 (MQ=255)                             ccGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGTTTTATTCGCCCCCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGaa                                                                                                                                                                                                                   <  2:333952/250‑1 (MQ=255)                              cGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGGAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGGGATGAACAAGATCGATATGCTGGa                                                                                                                                                                                                                    >  2:17404/1‑249 (MQ=255)                                  gCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGAtt                                                                                                                                                                                                               <  2:205570/250‑1 (MQ=255)                                        gTCGGTGAAACCGTACTTGCAGATTCCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGGGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAAcc                                                                                                                                                                                                        <  2:562628/251‑1 (MQ=255)                                           ggTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACcgcg                                                                                                                                                                                                     <  1:486122/251‑1 (MQ=255)                                             tGAAACCGGACTTGAAGAGACCGTAGGGCTGATTCACCACATGCCGCACGATCTGGTGGCGGCATTTAAAGACACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTACGTGGACACGAAAAAAGCGAGACGGTGAATA‑CGGGACTTGGAGAGATCGACCATCTAGAGCTCCCAAATCTCCTGGGGATGAACAAGACCGATCTGcaggcaaacttagaaccgcgat                                                                                                                                                                                                   >  2:414068/1‑230 (MQ=255)                                              gAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCCCGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTAt                                                                                                                                                                                                  >  1:241406/1‑251 (MQ=255)                                              gAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTAt                                                                                                                                                                                                  <  2:146631/251‑1 (MQ=255)                                                   cGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGGATTGATc                                                                                                                                                                                             >  1:147502/1‑251 (MQ=255)                                                      aCTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGAGCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCggg                                                                                                                                                                                          <  2:491544/251‑1 (MQ=255)                                                         tgctgATACCGTAGGGTTTATTCGCCACCTGCCGCACGTACTGGCGGCTGCATTTAAAGCCACTTTACAAGAGACGAGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGa                                                                                                                                                                                         <  2:400833/245‑1 (MQ=255)                                                            aGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTTAAGGGATCGACGCTCACGAGATCCCCACTCTGCTGGGGATGAACAAGATCGATCTGCTGGAAGATTTCGAACCGCGTATTGATCGGGACgc                                                                                                                                                                                      >  1:493885/1‑248 (MQ=255)                                                             gATACCGTAGGGTTTATTCGCCAGCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGAGGCGGCGGATGTGCGTGTACAAGAAAACAGCGAAGCGGTGAATA‑CGGTTCTTGAAGAGAACGACGCTCGCGAGATCGCAACGCTGCTGGTGATGATCAAGGTCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAaga                                                                                                                                                                                   <  1:522147/251‑1 (MQ=255)                                                             gATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACACTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCTAACCGCGTATTGATCGGGACGAaga                                                                                                                                                                                   <  2:90460/251‑1 (MQ=255)                                                                aCCGTAGGGATTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGCGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGCAGCGGTGAATA‑CGGTTCTTGAAGAGGTCGACGCTCACGAGATTCCAACTCTGCTGGTGATGAACAAGATCGATATTCTGGAAGATTTCGAACCGCGTATTGATCGGGGCGAAgaga                                                                                                                                                                                 >  2:352931/1‑250 (MQ=255)                                                                      gggTTTATTCGCCCCCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaacc                                                                                                                                                                          <  1:253349/251‑1 (MQ=255)                                                                      gggTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaacc                                                                                                                                                                          >  1:465209/1‑251 (MQ=255)                                                                       ggTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaaccg                                                                                                                                                                         >  1:471724/1‑251 (MQ=255)                                                                         tttATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGGGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGGTCGGGGCGAAGAGAACAaaccg                                                                                                                                                                         >  2:903/1‑249 (MQ=255)                                                                         tttAGTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaaccg                                                                                                                                                                         <  1:903/249‑1 (MQ=255)                                                                           tATTCGCCACCGGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaaccgaac                                                                                                                                                                      <  2:49091/250‑1 (MQ=255)                                                                             ttCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACACGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTCTTGATCGGGACGAAGAGAACAAACCGAACCgt                                                                                                                                                                   >  1:376082/1‑251 (MQ=255)                                                                              tCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCgt                                                                                                                                                                   <  1:528606/250‑1 (MQ=255)                                                                               cGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGCTGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGCGATCCCAACTCTGCTGGTGATGAACAAAATCGATATGCTGGAAGATTTCGACCCGCGTATTGATCGGGACGAAGAGAAAAAACCGAACCgtgt                                                                                                                                                                 >  2:96729/1‑251 (MQ=255)                                                                                   aCCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGACAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTgg                                                                                                                                                             >  1:329928/1‑251 (MQ=255)                                                                                   aCCTGCCGCACGATCTGCTAGCGGCATTTAAAGACACGTTACAAGAGCCGCGGCAAGCCACATTACTGCTGCACGTAATTGCTGCGGCGGATGAGAGCGTACAAGCAAACATCGAAGCGGTGAATA‑CGGTGCTTGAAGAGAACGACGCTCACGAGCTCCCAAATCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGACACGCGTATTGACGGGGACGAAGAGAACAAACAGCAACGTGTCTgg                                                                                                                                                             >  2:346513/1‑251 (MQ=255)                                                                                        ccGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGGTGTGCGGGTACAAGAAAACATCGAAGCGGGGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGGGATGAACAAGATCGGTATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAAAAAACCGAACCGTGTCTGGCtttt                                                                                                                                                        >  2:458125/1‑250 (MQ=255)                                                                                          gCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGAAGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGCAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATCTGCTGGAAGATTTCGAACCGCGTATTGATCGGGCCGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCg                                                                                                                                                      >  1:557119/1‑251 (MQ=255)                                                                                             cGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAAATCTGCTGGTGATGAACAAGATCGATATGATGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGGGAACCAACCGAACCGTGTCTGGCTTTCCGca                                                                                                                                                    >  1:496820/1‑250 (MQ=255)                                                                                                    gtggCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCgg                                                                                                                                            <  2:127440/251‑1 (MQ=255)                                                                                                    gtggCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTAAAAGAAAAAATCGAAGCGGGGAATA‑CGGTTTTTGAAGAGGTCGACGCTCACGAGATCCCAACTCTGCTGGGGATTAACAAGATAGGTATGATGGGAGATTTTGAAACGGGTATTGATCGGGACGAAGGGAACAAAACGAACCGGGTCTGGCTTTcccaacaaaccgg                                                                                                                                            >  2:425659/1‑240 (MQ=255)                                                                                                               tAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAg                                                                                                                                                 <  1:432404/235‑1 (MQ=255)                                                                                                               tAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGGGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGAAAGATTTCGCACCGCGTATTGATCGGGACGAAGGGAACAAACAGAACCGGGTCTGGCTTTCCGccccgagaacggaagagcggc                                                                                                                                 >  2:432404/1‑231 (MQ=255)                                                                                                                   gCCACGTTACAAGAGACGCGGCAAGCAACATTACTGCTGCACGTCATTGATGAGGCGGATGTGCGTGGACAAGGCAACGTCGAAGCGGTGAAAA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATACCAAATCTGCTGGTGATGAACACGGTCGGTATGGTGGAAGATTTCGAAACGCGTATTGGTCGAGAAGAAGGGGACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGGGCGGGGAGACcac                                                                                                                              >  1:356004/1‑250 (MQ=255)                                                                                                                       cGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGGGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTTGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACAGTGTCTGGCTTTCCGCACAGGCCGGGGCGGGGATACCACC‑GCtt                                                                                                                        >  2:210378/1‑251 (MQ=255)                                                                                                                           aCAAGAGACGCGGCAAGCCACAATACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAAACTCAAAGCGGTGAATAACGGT‑ATTGGAGAGAACGGAGCTCACGAGATCCCAACTCTGCAGGGGGTGAACAAGATTGATATGATGGAAAATTTTGAACCGCGTATTAATCGGGAAGAAGAGAACAAACCGAAACATGGCTGGCTTTCAGCACAGTCCGGCGCGAGGATCCCAAA‑GAttttt                                                                                                                     >  2:330949/1‑250 (MQ=255)                                                                                                                                 gaCGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACACGATCGATATGCTGGAAGATTTCGAACCGCGTATTTATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACA‑GCTTTTTCCGGCtt                                                                                                              >  1:404930/1‑251 (MQ=255)                                                                                                                                   cgcgGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTg                                                                                                            <  2:441055/251‑1 (MQ=255)                                                                                                                                         aaGCCACAGTACTGCTGCACGTCATTGAGGTGTCGGATGTGCGTGTACAAGAAAACATCTAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAATAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGCTACCACA‑GCTTTTTCAGGCGTTGACGGAg                                                                                                      <  2:479366/251‑1 (MQ=255)                                                                                                                                          aGCCACATTACTGCTGCACGTCATTGATGCGGCGGATTTGCGTGGACAAGAAAACCTCCAAACGGTGAATA‑CGGGTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAAATCGATATGATGGGAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCCAACCGTGTCTGGCTTTCCGCCCAGAACGGAGCGGGGATACC‑CACGCTTTTTCCTGCTTTGACGGAGc                                                                                                     >  2:154042/1‑251 (MQ=255)                                                                                                                                              acaTTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCt                                                                                                 <  2:138405/251‑1 (MQ=255)                                                                                                                                               caTTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGGGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCTGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCtt                                                                                                <  2:554245/251‑1 (MQ=255)                                                                                                                                                aTTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTTAGGCTTTGACGGAGCGGCttt                                                                                               >  2:31947/1‑251 (MQ=255)                                                                                                                                                      gctgcACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTGTCAGGCTGTGACGGAGCGGCTTTCCGGCg                                                                                         <  2:119982/251‑1 (MQ=255)                                                                                                                                                      gctgcACGTCATTGATGCGGCGGATGGGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCTGGGATACCACC‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCg                                                                                         <  1:414068/251‑1 (MQ=255)                                                                                                                                                          cACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGt                                                                                     <  2:471724/251‑1 (MQ=255)                                                                                                                                                                   gATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCgcagc                                                                             <  1:31947/250‑1 (MQ=255)                                                                                                                                                                      gcggcgGATGTGCTTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATAc                                                                         <  2:440624/251‑1 (MQ=255)                                                                                                                                                                       cggcggATGGGCGTGTACAAGAAAACATCGAAGCTGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGTTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATGGATCGGGACGAAGAGAACAAACCGAGCCGTGTCTGGCTTTCCGCACAGTCCGGAGCGGGGATACCACA‑GCTGTTTCAGGCTTTGACGGAGCGGCTTGCCGTCGAGGTGGCGTGGCATACa                                                                        <  2:221148/251‑1 (MQ=255)                                                                                                                                                                         gcggATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACAtt                                                                      <  1:75994/251‑1 (MQ=255)                                                                                                                                                                          cggATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCtt                                                                                                                        >  1:31975/1‑200 (MQ=255)                                                                                                                                                                          cggATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCtt                                                                                                                        <  2:31975/200‑1 (MQ=255)                                                                                                                                                                            gATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCCGGCTTTGCCGGAGCGGCTTTCCGGCGAGGTGGCgcagactacctttcg                                                                   >  2:257375/1‑240 (MQ=255)                                                                                                                                                                            gATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCg                                                                   <  1:535549/251‑1 (MQ=255)                                                                                                                                                                               gtgCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCTGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGTCTTTCCGGCGAGGTGGCGCAGCATACATGGCGTc                                                                 <  2:262571/250‑1 (MQ=255)                                                                                                                                                                               gtgCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCt                                                                >  1:262142/1‑251 (MQ=255)                                                                                                                                                                                ggCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCGCAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTg                                                               <  2:111455/250‑1 (MQ=255)                                                                                                                                                                                 gCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGCCTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGACGAGAACCAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGc                                                              >  1:220810/1‑251 (MQ=255)                                                                                                                                                                                     gtACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCAc                                                           <  1:367386/250‑1 (MQ=255)                                                                                                                                                                                      tACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGAGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCg                                                         >  2:553553/1‑251 (MQ=255)                                                                                                                                                                                       aCAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCTCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCg                                                         <  1:2666/250‑1 (MQ=255)                                                                                                                                                                                          aGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAgg                                                     <  2:194052/251‑1 (MQ=255)                                                                                                                                                                                            aaaaCATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGaa                                                   <  2:297818/251‑1 (MQ=255)                                                                                                                                                                                              aaCATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAgg                                                 <  1:386321/251‑1 (MQ=255)                                                                                                                                                                                               aCATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAggg                                                <  1:49719/251‑1 (MQ=255)                                                                                                                                                                                               aCATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAggg                                                <  1:96729/251‑1 (MQ=255)                                                                                                                                                                                                    gAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCt                                           >  1:493684/1‑251 (MQ=255)                                                                                                                                                                                                    gAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCAAACTATGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCt                                           >  1:377626/1‑251 (MQ=255)                                                                                                                                                                                                     aaGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTg                                          <  2:163839/251‑1 (MQ=255)                                                                                                                                                                                                      aGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTgg                                         >  2:176758/1‑250 (MQ=255)                                                                                                                                                                                                           tGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAgg                                    >  2:113581/1‑250 (MQ=255)                                                                                                                                                                                                           gtgaatccgtttcctgaaaggaTCGACGCTCACGAGTACCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAATATTTCGAACCGCGTATCGATCGGGACGAAGAGATCAAACCGAACCGTGTCTGCCTTTCCGCCGAGACCGGAGCGGGGATATCCCA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGCTGACGCATCCTACATTGCGTCTGACTCGGTATGAAGGCCGTCTGAGAAg                                     <  2:254240/231‑1 (MQ=255)                                                                                                                                                                                                            gAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGGCCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTc                                   <  1:481015/251‑1 (MQ=255)                                                                                                                                                                                                                 acggggcTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCGTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGttt                               <  2:155561/245‑1 (MQ=255)                                                                                                                                                                                                                  cGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGtttt                              <  1:183048/251‑1 (MQ=255)                                                                                                                                                                                                                   ggTTCTTGAAGAGATCGACGCTCAAGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGGTACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGttttt                             <  2:456999/251‑1 (MQ=255)                                                                                                                                                                                                                     ttCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTAt                           >  1:344334/1‑251 (MQ=255)                                                                                                                                                                                                                         tGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCa                         <  1:180741/249‑1 (MQ=255)                                                                                                                                                                                                                              agaTCGACGCTCACGAGATCCCCACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGGCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGACGTTTTTATCAGCTTc                    >  1:323059/1‑249 (MQ=255)                                                                                                                                                                                                                              agaTCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTc                    >  1:506985/1‑249 (MQ=255)                                                                                                                                                                                                                              agaTCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGGATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTGTTATCAGCTTCAg                  <  2:493885/251‑1 (MQ=255)                                                                                                                                                                                                                                aTCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCTCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGCGAAGTCGTTTTTATCAGCTTCCGGc                >  2:22335/1‑251 (MQ=255)                                                                                                                                                                                                                                     cGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGCTATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGACGTCGTTTTTATCAGCTTCAGGCAAt             >  2:301301/1‑249 (MQ=255)                                                                                                                                                                                                                                          aCGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGaaaa       >  2:450817/1‑250 (MQ=255)                                                                                                                                                                                                                                              gaTCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGt  <  2:431828/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   ACCCTCGACCCGACGTTGCGGCGTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATA‑CGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACA‑GCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGT  >  NC_000913/4401362‑4401848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑