| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,388,120 | C→T | A417A (GCG→GCA) | mscM ← | miniconductance mechanosensitive channel MscM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,388,120 | 0 | C | T | 98.2% | 383.9 / NA | 113 | A417A (GCG→GCA) | mscM | miniconductance mechanosensitive channel MscM |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (52/58); minor base G (1/1); total (54/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 113 total aligned reads displayed.
GAAAACGGGTGAAATAGCGGCGCGAGTAAATACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATC > NC_000913/4387873‑4388362 | gAAAACGGGTGAAATAGCGGCGCGAGTAAATACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCa < 2:122042/251‑1 (MQ=255)gAAAACGGGTGAAATAGCGGCGCGAGTAAATACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCa < 1:429221/251‑1 (MQ=255) aaCGGGTGAAATAGCGGCGCGAGTAAATACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCAtcc < 2:253452/251‑1 (MQ=255) gggTGAAATAGCGGCGCGAGTAAATACTGCAACCGCACAGAATCAACGCGCCCAACAGCGGCAAAATTGTCTCTTTGCTGGCCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGCCGCAGATCCTGGGCGTTTTCCAGAGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGAGCGTTGCTTCGTTCACCTCTTTCAGTGCAtcctcc > 1:16168/1‑251 (MQ=255) ggTGAAATAGCGGCGCGAGTAAATACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCAtcctcc > 1:60661/1‑250 (MQ=255) gTGAAATAGCGGCGCGAGTAAATACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCAtcctcc > 1:572577/1‑249 (MQ=255) aaaTAGCGGCGCGAGTAAATACTGCAACCGACCAGAAGCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGTTCAGCATCATCACACTGGCTTTGCCCAGCTGGCTGAAGGTGTCCAGCGAAATGAGACGACTCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGTTCATCGGGCGCACGTCAGAGGTTTAGATCAGATAGTGGTGCGTTGCTTCTTTCACCTCGTTCAGTGCAtcctcaatttg < 2:39811/251‑7 (MQ=255) aTAGCGGCGCGAGTAAATACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGcc < 1:43677/251‑1 (MQ=255) aTAGCGGCGCGAGTAAATACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTGGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCTCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGcc < 1:301409/251‑1 (MQ=255) gAGTAAATACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGAc < 1:238670/251‑1 (MQ=255) aGTAAATACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACt > 1:397226/1‑251 (MQ=255) gTAAATACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACtt < 1:231019/251‑1 (MQ=255) aaTACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCACAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCCGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGGCCAGACCAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTc > 2:560799/1‑250 (MQ=255) aTACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTc > 2:220311/1‑249 (MQ=255) aTACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTATCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTc > 1:97322/1‑249 (MQ=255) tGCAAACGACCAGAATCAACACGCCCAACAGCGGCGAAATTGTCTATTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCCGCTTg > 2:334338/1‑251 (MQ=255) aaCCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTc < 1:438081/251‑1 (MQ=255) aCCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCa < 1:473066/251‑1 (MQ=255) cGACCAGAATCCACGCGCCAAACATCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGt > 2:491104/1‑251 (MQ=255) gACCAAAATCACCGCGACAAACAGCGGCAAAATTGTCTCTTTGCAGGTCCGCAGCATCACACTGGCTTTGCCAAACTGACTGAAGGTGTCCAGCGAAATGCGACGACGCAGCTCCTGGGCGATTTACAGCGGCCAGGCGACGGACATCGGGCGCACGTCAGAGGTCCAGAACAGATCGCGGCGCGGTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGtt > 2:285673/1‑251 (MQ=255) cAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTcc > 1:254553/1‑250 (MQ=255) aaTCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAAt > 1:493763/1‑250 (MQ=255) acAACGCGCCAAACAGCGGCAAAATTGTCTCTGTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTTGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCCCGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGGTGCCC‑GTGGGAGACGTGCAGCTTTGTCAGTTCCAAtagt < 2:367526/250‑1 (MQ=255) aaCGCGCCAAACAGCGGCCAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGCTAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCA‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAAtagt > 2:301237/1‑249 (MQ=255) aaCGCGCCAAACAGCGGCAAAAATGTATATTTGCTGGTAAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGCAATGAGACGACGCAGATCATGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAAtagt > 2:280235/1‑249 (MQ=255) cgcgCCAAACAGAGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCACTAGTAGCg > 2:583282/1‑251 (MQ=255) gcCAAACCGCGGCAAAATCGTCTCGTGGCGGGTCAGCATCATCACACGGGCTTTGCCCAACGGACTGAAGGTGGCCAGCGAAATGAGACGCCGCAGTTCCTGGGCGATTGCCAGCGGCCTGTCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTGCAGTGCATCCTCGAGTGGCCC‑GTTGGAGACTGACAGCTTGGTCGGCTCCAAGAGTAGCgtgt < 2:559862/251‑1 (MQ=255) aaCAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGcc > 1:494341/1‑251 (MQ=255) cGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCt > 1:508877/1‑251 (MQ=255) ggCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGTCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCGCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATATTATCGTGTCGCCACCCTg < 2:525090/251‑1 (MQ=255) aaaaTTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGca > 1:440760/1‑250 (MQ=255) tctTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGtt > 1:160004/1‑251 (MQ=255) ttGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCTATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCGCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCa < 1:250699/250‑1 (MQ=255) cAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCAc < 1:473712/251‑1 (MQ=255) gCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGc > 2:200174/1‑251 (MQ=255) atcatcACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTg < 2:60661/251‑1 (MQ=255) atcaCACTGGCTTTGCCCCACTGACTGAAGGTGTCCAGCGAAATGAGCCGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGACCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGt > 2:59654/1‑251 (MQ=255) cTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTgcgc > 2:529427/1‑249 (MQ=255) tGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCCGCAACTCACGCTGAGTGCGCAGt > 1:306840/1‑251 (MQ=255) ggCTTTGCCCAACTGTCGGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGt < 2:542137/250‑1 (MQ=255) ggCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGtt < 1:106303/251‑1 (MQ=255) ggCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGt < 1:328798/250‑1 (MQ=255) cTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTtgt > 1:355640/1‑251 (MQ=255) ttGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTtgtg < 1:293732/250‑1 (MQ=255) gCCCAACTGACTGAAGGTTTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGTTCCAGAACAGATAGCGGTGCGGTTCGTCGTTCACCTCTATCAGTGCATCCTCCAGTTGCCC‑GTTGGAGATTTTCAGCTTGGTCAGTTCCAATAGTATTGTGTCGCCTCCCTGCAACAA‑TGAGTTCAGCATCTCGCGCTGAGTGCTCAGTTGTGCt < 2:559662/250‑1 (MQ=255) gCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCt < 2:446109/250‑1 (MQ=255) ccAACTGACTGAAGGAGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTcc > 2:216364/1‑251 (MQ=255) ggaAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGGTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACgg < 2:63249/250‑1 (MQ=255) gaAGGTGTCGAGCGAAATGAGAAGAGTCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCCTCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGGGTTGATTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGGCAGTTCCAAGAGTAGCGTGTCGCCACCCTGCAACAA‑GGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGt < 2:458817/251‑1 (MQ=255) aaGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGtt < 2:440760/251‑1 (MQ=255) gtCCAGCTAAATGAGACGACGTAGATCCTGGGCGATTTCCAGCTGCCAGGCGATGGTCAGCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCAGCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTGGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTTAGCAACTCACGCTGAGTGCGCAGGTGTGCTTCCAGAATTCTGTTTTGc < 2:354508/250‑1 (MQ=255) gccAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTc < 1:334338/250‑1 (MQ=255) cAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCgg < 2:170205/251‑1 (MQ=255) aTGAGACGACGCAGATCCTGGGCGAATTCCAGCGGCCAGGCGATGGTCATCCGGCGCACGAAAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCAAGTAGCAC‑GTTGGAGACTTTCAGCTTGGCCAGTTCCAATAGAAGCGTGTCGCCAACCTGCCACAC‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGCGCTTCCAGAAAACGGGTTTGCTCGGCAGTc > 2:567812/1‑249 (MQ=255) acgacgCAGATCCTGGTAGATTTACAGCGGCCTGGCGATGATCATCGGGAGCCCGTAAGAGGTCCCGAACAGATAGCGGCGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCTGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAC‑TGAGACCCGCACCTCACGCTGGGTACGCAGTTGTGCTTCCAGAATCCGTTTTTGcgaggcggtccgcgtct > 2:28292/1‑235 (MQ=255) acgCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTTCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTTCTTCCAGAATACGGTGTTGCTCGGCAGTCAGCGGCTGAc < 2:577862/251‑1 (MQ=255) aGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTc > 1:143853/1‑251 (MQ=255) aGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGAGGCTGACCGTc > 2:430118/1‑251 (MQ=255) gATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGACCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCg < 1:59654/251‑1 (MQ=255) aTCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCgg > 1:105235/1‑251 (MQ=255) tCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGGGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGc > 1:51185/1‑251 (MQ=255) gCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCtgatg < 2:263158/251‑1 (MQ=255) gCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGACAGTCAGCGGCTGACCGTCGGCCtgatg < 1:298873/251‑1 (MQ=255) cGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCtgatga < 2:19661/251‑1 (MQ=255) ggtttCCATCGGCCAGGCGATGCTCATCGGGCGCACGTCCGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCCTCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCtgatga > 2:359054/3‑250 (MQ=255) aTTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAAt > 1:71388/1‑251 (MQ=255) ttCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAAtt < 1:430118/250‑1 (MQ=255) ttCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAAtt < 2:105235/250‑1 (MQ=255) ggCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTag < 1:226334/251‑1 (MQ=255) gCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGCTGAATTTGCCGTagc > 1:98633/1‑251 (MQ=255) cAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATCGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTagc > 2:475792/1‑249 (MQ=255) cAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTagc > 1:551985/1‑249 (MQ=255) gCGATGGTCAGCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCGGACCGTCGGCCGGATGAATTTGCCGTAGCAGCgg < 2:66453/251‑1 (MQ=255) cGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGCTGAATTTGCCGTAGCAGCGGc > 1:17163/1‑251 (MQ=255) ggatggcATCGGGCGCACGTCAGAAGGCCAGAACAGGTAACGGTTCGTTGCCTCGTTCACCTTCTTCCGTGCATCCTCCAGTTGCCCCGTGGGAGACTTCCA‑CGTGGGCAGGTCCAAAAGTTGCGTGGCGGCACCCCGCAACAAATG‑GGTCATCAACTCACGCCGAGTGCGCAATTGGTCCTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGAGGAATTTGCCGTAGCATCGGCt < 2:239416/245‑1 (MQ=255) gATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCt > 2:267841/1‑251 (MQ=255) tCATCGGGAGCACGTCAGAGGTACAGGACAGATAGAGGAGAGTTGCTTCGTTCACCTCTTTCAGTGCTTCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TAAGTTCAGCAACTCACACTGAGTGCGCTGTTGTGCTCCCATAATACGGTTTTGCTCTGCAGTCAGCGGCTGCACGTCGGCCTGATGAATTTGCCTTAGCAGCGGCTGttt > 2:412596/1‑250 (MQ=255) cATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTttat < 2:160004/251‑1 (MQ=255) aTCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTttatt < 2:493763/251‑1 (MQ=255) gcgcACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCATCTCACGCTGAGTGCGCAGTTGTGCGTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCa < 1:540105/251‑1 (MQ=255) cACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTGTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAt < 2:52610/251‑1 (MQ=255) agagGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGAATTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGCTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCAt < 1:320401/251‑1 (MQ=255) gagGTCCAGCACAGATAGCGGAGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGAAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCAt > 2:103457/1‑250 (MQ=255) aCAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGt > 1:572085/1‑251 (MQ=255) aCAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGt < 2:401752/251‑1 (MQ=255) aGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGt > 2:298272/1‑207 (MQ=255) aGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGt < 1:298272/207‑1 (MQ=255) gATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGc < 1:560799/251‑1 (MQ=255) ggTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGc > 2:204700/1‑251 (MQ=255) gTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGc > 1:513093/1‑250 (MQ=255) gTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTg < 1:258851/251‑1 (MQ=255) ttCGTTAACCTCTTTCAGGGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCGTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGGGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGGTGACCGTCGGCCTGATGAATTTTCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGccc > 2:102080/1‑250 (MQ=255) ttCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTc > 1:337122/1‑251 (MQ=255) ttCACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTc > 1:119532/1‑251 (MQ=255) ttCACCTCTTTCAATCCATCCTCCAGTTCCCC‑GTTACAGACTTTCAGCTTCATCACTTCCCCTATTAGCTTCTCGCCTCCCTTCAACAA‑TGATTTCAGCAACTCACCCTTTGTGCGCTATTTTGCTTCCAGAATACGGTTTTTCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTgcgcaat < 1:148014/248‑4 (MQ=255) tCACCTCTTTCAGTGCATCCTCCAGCTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCg > 1:269479/1‑251 (MQ=255) cACCTCTTTCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAGCGTAACCGGTGCACACGCAACTGCGCCATTTCg < 2:397226/250‑1 (MQ=255) cACCTCTTTCAGGGCCTCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCgg < 2:285038/251‑1 (MQ=255) ttCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATc < 1:529427/248‑1 (MQ=255) gtCAGTGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATGCGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCTTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACTGAACCGTGGCACACGCAACTGCGCCATTTCGGTATc < 2:71388/247‑1 (MQ=255) | GAAAACGGGTGAAATAGCGGCGCGAGTAAATACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCC‑GTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA‑TGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATC > NC_000913/4387873‑4388362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |