Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,373,421	C→T	A272T (GCG→ACG)	yjeJ ←	protein YjeJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,373,421	0	C	T	100.0%	229.3 / NA	68	A272T (GCG→ACG)	yjeJ	protein YjeJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/32);  total (36/32)

GGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGCGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAATGGTCGAACAAGT  >  NC_000913/4373174‑4373666                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       ggCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGcgtgcg                                                                                                                                                                                                                                                     >  2:177700/1‑251 (MQ=255)     gggTGAAAAACGGATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTa                                                                                                                                                                                                                                                   >  2:149269/1‑249 (MQ=255)       gTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCa                                                                                                                                                                                                                                               <  2:28132/251‑1 (MQ=255)        tGACAAACGAATAATGGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGAAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCCGCGTGCGTAAGCAt                                                                                                                                                                                                                                              >  2:487063/1‑251 (MQ=255)         gACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCAtt                                                                                                                                                                                                                                             >  1:286245/1‑251 (MQ=255)                     aTTGCCTGATGCGCTTCGCTTATGAGGCCTACATAAGGCAGTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCTGCATGACCAACGATCACGTGATCAGTCATTTCAGGCTTGATTGCTCCAGCCTCGCATGAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTTCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTa                                                                                                                                                                                                                                 <  2:292907/251‑1 (MQ=255)                        gCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGAGAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGc                                                                                                                                                                                                                               <  1:395101/250‑1 (MQ=255)                            gATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTg                                                                                                                                                                                                                          <  1:32981/251‑1 (MQ=255)                                      gcttATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGTTGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTc                                                                                                                                                                                                                >  1:300594/1‑251 (MQ=255)                                          aTCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTcgccg                                                                                                                                                                                                            >  2:297067/1‑251 (MQ=255)                                                ccTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACgg                                                                                                                                                                                                      >  1:247504/1‑251 (MQ=255)                                                       aaGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAAc                                                                                                                                                                                              >  1:159749/1‑251 (MQ=255)                                                          gCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGCTGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACAt                                                                                                                                                                                            >  2:312002/1‑250 (MQ=255)                                                               gCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGAcc                                                                                                                                                                                       <  1:70068/250‑1 (MQ=255)                                                               gCAACTGATTGAATGCTTGAGAATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGAcc                                                                                                                                                                                       <  1:203704/250‑1 (MQ=255)                                                                         gAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCATGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCTTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGcac                                                                                                                                                                            <  2:346354/251‑1 (MQ=255)                                                                         gAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGACATTTCAGGCTGGATGGCTCCAGCCTCGCCTTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑GGCGAACCTAGACCTGACATGGcac                                                                                                                                                                            >  1:311137/1‑251 (MQ=255)                                                                           aTGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACTCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTGAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCacac                                                                                                                                                                          <  2:257986/251‑1 (MQ=255)                                                                           aTGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCacac                                                                                                                                                                          <  1:543920/251‑1 (MQ=255)                                                                             gCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACAccc                                                                                                                                                                        <  1:569921/251‑1 (MQ=255)                                                                             gCTTGAGAATTTGTAGGCCGGATAAGGCGGTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACAccc                                                                                                                                                                        <  2:123663/251‑1 (MQ=255)                                                                               ttGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCcgc                                                                                                                                                                      >  1:467012/1‑251 (MQ=255)                                                                                         gTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCCCACCCGCGAGTTTTTTc                                                                                                                                                            >  1:288320/1‑251 (MQ=255)                                                                                           aGGCCGGATAAGGGGTTTACACCGAATCCGGCATGCCTAACGATCACGTTATCAGTCATTTCAGGCTGGATGTCTCCAGCCTCGCATTAGCAAAGGTGAGATTATTTAGCCTGTGGTGACGTTTTGCTGGTGGATTGAACACGTACGTGATGCAGAGTGCGTAAGCCTCGATCCTGGGTTAGCGGTTGGGCGCTATTCGCCGAAAAGT‑TACGAACATAGACCTGACATGGCCAACCCGCACGtttttgca                                                                                                                                                           >  2:496656/1‑247 (MQ=255)                                                                                           aGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTc                                                                                                                                                            >  1:77484/1‑249 (MQ=255)                                                                                             gCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTc                                                                                                                                                        <  1:100555/251‑1 (MQ=255)                                                                                                 gATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTgg                                                                                                                                                    >  1:3204/1‑251 (MQ=255)                                                                                                                  gcATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTACGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACACGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTc                                                                                                                                   >  1:337560/1‑251 (MQ=255)                                                                                                                  gcATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTc                                                                                                                                   >  1:171581/1‑251 (MQ=255)                                                                                                                  gcATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTc                                                                                                                                   <  1:4974/251‑1 (MQ=255)                                                                                                                                  aCGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTc                                                                                                                   >  1:316020/1‑251 (MQ=255)                                                                                                                                    gATCACGTTAGCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATGTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTa                                                                                                                 <  1:1410/251‑1 (MQ=255)                                                                                                                                     ctCACGTTGTCAGCCATTTCAGGCTGGATGGCTCCAGCCTCCCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGCTGCAGCGTGCGTAAGCATTGCTCCTGGGTTAGAGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATCGACCTGACATGGCACCCCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCtgt                                                                                                                >  2:195318/2‑249 (MQ=255)                                                                                                                                          gTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCtt                                                                                                           >  1:326464/1‑251 (MQ=255)                                                                                                                                                aGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCg                                                                                                     >  1:172499/1‑251 (MQ=255)                                                                                                                                                   cATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCg                                                                                                  >  1:405116/1‑251 (MQ=255)                                                                                                                                                        cAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCAGTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTgat                                                                                             <  2:189024/251‑3 (MQ=255)                                                                                                                                                             tGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGGGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCAcg                                                                                        >  2:280798/1‑251 (MQ=255)                                                                                                                                                             tGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCAcg                                                                                        >  1:330821/1‑251 (MQ=255)                                                                                                                                                                  ggCTCCAGCCTCGCATTAGCAAATATGAGATTATTTCGCCTGTGGTGCTGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTATCGGTTGGGCGTTATTCGCCTCTACGG‑TGCGAACATAGACCTTACATGGCACATCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCTAGTTATCGCTTCTATTTCTGTACCCGGCGCTGCTTCACGCGttt                                                                                   <  2:359252/251‑1 (MQ=255)                                                                                                                                                                              gCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTgcgc                                                                       >  2:239792/1‑251 (MQ=255)                                                                                                                                                                                       aggATGAGATTATTTCGCCTGTGGGGCAGTTTTTCTGGTGGCTTGAACGCGTAAGTGGTGCCGGGTGCGCAAGCATTGATCCGGGGTTAGCGGGTGGTCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACCCCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGGTGCTTCACGCGTTTTCACCAGTgcgtagctacatt                                                              <  2:466781/249‑11 (MQ=255)                                                                                                                                                                                          aTGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGtt                                                           >  1:198681/1‑251 (MQ=255)                                                                                                                                                                                                  attTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGcc                                                    <  1:415354/250‑1 (MQ=255)                                                                                                                                                                                                  attTCGCCTGTGGGGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGcc                                                    <  2:172499/250‑1 (MQ=255)                                                                                                                                                                                                      cGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACttt                                               >  1:318331/1‑251 (MQ=255)                                                                                                                                                                                                          tgtgGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACt                                                                           >  2:537275/1‑219 (MQ=255)                                                                                                                                                                                                             ggtgCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCttt                                        >  2:158033/1‑251 (MQ=255)                                                                                                                                                                                                                  aGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGc                                   <  1:446173/251‑1 (MQ=255)                                                                                                                                                                                                                     tttGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCATGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGt                                >  1:363493/1‑251 (MQ=255)                                                                                                                                                                                                                       tGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTa                               <  1:297067/250‑1 (MQ=255)                                                                                                                                                                                                                          tggtggATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGc                           >  2:276150/1‑251 (MQ=255)                                                                                                                                                                                                                          tggtggATTCAACACGTAAGTGATGCAGCGTGAGTAAGCATCGATCCAGGGTCCGCGGTTGGGCGTTATTCGCCGC‑ACGGGTGCGAACATAGACCTGCCATGGCAAAACCGCGAGTTTTTTCAGCCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTACATTGATCTTCGCCTCTTTCTTCTTTAACGCGGtcactc                           >  2:550382/1‑246 (MQ=255)                                                                                                                                                                                                                               gATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTGTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGc                      <  2:286245/251‑1 (MQ=255)                                                                                                                                                                                                                                  tGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTc                                                                                                                                   >  2:439439/1‑139 (MQ=255)                                                                                                                                                                                                                                  tGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTc                                                                                                                                   <  1:439439/139‑1 (MQ=255)                                                                                                                                                                                                                                   gAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGcc                     >  1:131089/1‑248 (MQ=255)                                                                                                                                                                                                                                    aaCACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCTAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGAt                 <  1:220090/251‑1 (MQ=255)                                                                                                                                                                                                                                     acacGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTa                               >  2:374326/1‑236 (MQ=255)                                                                                                                                                                                                                                     acacGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTa                               <  1:374326/236‑1 (MQ=255)                                                                                                                                                                                                                                       ccGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTGAGCGGGTGGGCGTTATGCGCCGCAACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATGCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTGGTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAAt              <  1:482425/250‑1 (MQ=255)                                                                                                                                                                                                                                       acGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAAt              >  1:468030/1‑251 (MQ=255)                                                                                                                                                                                                                                         gTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGTTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTGCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTTCCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAATgg            <  1:81530/251‑1 (MQ=255)                                                                                                                                                                                                                                           aaGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAATGGTc          <  1:279930/251‑1 (MQ=255)                                                                                                                                                                                                                                           aaGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑GGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAATGGTc          <  2:330821/251‑1 (MQ=255)                                                                                                                                                                                                                                            aGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAATGGTCg         <  2:77484/251‑1 (MQ=255)                                                                                                                                                                                                                                                 tGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACACGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCt                                          >  2:190541/1‑213 (MQ=255)                                                                                                                                                                                                                                                 tGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACACGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCt                                          <  1:190541/213‑1 (MQ=255)                                                                                                                                                                                                                                                   cAGCGTGCGTAAGCATTGATCCTGGGCTAGCGGTTGGGCGTTATTCGCCGCTACGG‑GTCGAACATAGACCTGACATGGCACACCCGCGAGTTTTGTCAGTCGTGGGCTGAAATCAAGCATTCGGTGAGTGATCGCTTCTATTTCTTTACCCGTCGATGCTTCACGCGTGTTCACCACTGTGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAGGCGGGAAAGCTCAGCCAGATAATGGTCTAACAAGt  <  2:364896/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       GGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGCGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGG‑TGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAATGGTCGAACAAGT  >  NC_000913/4373174‑4373666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑