Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,366,668	C→T	A36T (GCC→ACC)	dcuA ←	C4‑dicarboxylate transporter DcuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,366,668	0	C	T	100.0%	113.7 / NA	35	A36T (GCC→ACC)	dcuA	C4‑dicarboxylate transporter DcuA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/11);  total (24/11)

AACTGGCAGTGTCGCCAGAGAGATGTTGCC‑AGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGT  >  NC_000913/4366432‑4366911                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    aaCTGGCAGTGTCGCCAGAGAGATGTTGCC‑AGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCAccc                                                                                                                                                                                                                                       >  1:363873/1‑251 (MQ=255)  aCTGGCGGTGCCGCAAGAGAGA‑GTTGCCCAGTACAAGCAAAGAAAGTAAGAAAATGGGCCACGACCGGTGCGGGGTTGGTGATTTTTTCCGGGTTCGGGCGCAGCATTTTTTCTGTCTGA‑AAACCAAGATAGTCCAGCCCGCCAGAAACCTGCATGGAAAAAATGGCGGCAATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCAcccc                                                                                                                                                                                                                                      <  1:536958/251‑1 (MQ=255)       cAGTGTCGCCAGAGAGATGTTGCC‑AGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGCGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGATTTTCTGTCTGATGAACCA‑GCTAGTCCAGACCACCAGCAACCTGCATGGCAGAAATAGCGGCGGTAACCGCCATGATAATGGAGATGAAATCGAACGGGATGTTACCGGGTTTAACGCACATAGCGGTAAGAACCAGCACCCCCAATc                                                                                                                                                                                                                                 >  1:248291/1‑251 (MQ=255)        aGTGTCGCCAGAGAGATGTTGCC‑AGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGGGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGATTCACGCCAATAGCGGTAAGAACTAGCACACCCAATcc                                                                                                                                                                                                                                >  2:177306/1‑251 (MQ=255)               ccAGAGAGATGTTGCC‑AGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACAAGCACCCCCAATCCGCCTGc                                                                                                                                                                                                                          >  1:295613/1‑250 (MQ=255)               ccAGAGAGATGTTGCC‑AGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGCGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGc                                                                                                                                                                                                                          >  1:361635/1‑250 (MQ=255)                 agagagATGTTGCC‑AGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGcca                                                                                                                                                                                                                        >  2:123817/1‑248 (MQ=255)                    gagaTGTTGCC‑AGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAAcc                                                                                                                                                                                                                    >  2:456044/1‑251 (MQ=255)                           tGCC‑AGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCt                                                                                                                                                                                                             >  1:430346/1‑251 (MQ=255)                                          aaGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGCTGAACCA‑GATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGAGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCt                                                                                                                                                                                               >  2:552733/1‑251 (MQ=255)                                                  cAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGccc                                                                                                                                                                                        >  1:334685/1‑250 (MQ=255)                                                            gTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGACCAAGAAGATCGc                                                                                                                                                                             >  1:481681/1‑251 (MQ=255)                                                                                  tGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATc                                                                                                                                                                               >  2:197902/1‑227 (MQ=255)                                                                                   gATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTACCGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGACGATCGCCAGCAAAACTATGATGAGTTCt                                                                                                                                                       >  2:56367/1‑250 (MQ=255)                                                                                        aTTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACt                                                                                                                                                 >  2:516144/1‑251 (MQ=255)                                                                                                  aCGGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATAtt                                                                                                                                         >  2:480881/1‑249 (MQ=255)                                                                                                   cGGCGCAGCAGCTTTTATGTCTGATGAACCA‑GATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCACTCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGc                                                                                                                                      >  1:220656/1‑251 (MQ=255)                                                                                                              cTTTTCTGTCTGATGAACCA‑GATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGttt                                                                                                                           >  1:59645/1‑251 (MQ=255)                                                                                                                                             aCCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGCTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTAtttt                                                                                             >  1:477029/1‑251 (MQ=255)                                                                                                                                                 ccAGCAACCTGCAGGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAg                                                                                         <  1:480881/251‑1 (MQ=255)                                                                                                                                                      aacctgaaggaaaaaaaggggggcgATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCACCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACt                                                                                    <  1:290598/230‑1 (MQ=255)                                                                                                                                                                acaaaagtgtcggtggaaaCCGCCATGATAATGGAGATGACCTCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGTTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCGGGCGCCCAAGAATATCGCCAGCAAAACTATGAGGAGTTCTACAACTAGCATATTAGCCGTCCTTTTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGa                                                                          <  2:171739/234‑1 (MQ=255)                                                                                                                                                                  aGAAATGGCGGAGATAAACGCCATGATAATTGAGATGACATCGAACGGGATGTTAACGGGCTTAACGCCAATAGCGGTAAGAAACAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCACAATCTGGCGCCCAAGAAGATCGCCAGCACAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTCGATTGTATTTTTTAAGTTACTCCTCACAAGaa                                                                         >  2:94310/1‑250 (MQ=255)                                                                                                                                                                      aTAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGccttcct                                                                                                                                >  1:512391/1‑191 (MQ=255)                                                                                                                                                                        aGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGc                                                                  >  2:17180/1‑251 (MQ=255)                                                                                                                                                                                  accgcaatgctcaTGGACATGACATAGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGAACCCCCAATCCGCCTGCAAACCCAATACCTATTCCCCACAATCTGGCGCCCCAGAAGATCGCCAGCACAACTATGATGAGTTCTCCAACTAGCATATTAGCCTTCCTTGATTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTg                                                        >  2:37848/13‑251 (MQ=255)                                                                                                                                                                                   ccGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTa                                                                                                                                                                                                            <  1:166576/102‑1 (MQ=255)                                                                                                                                                                                   ccGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTa                                                                                                                                                                                                            >  2:166576/1‑102 (MQ=255)                                                                                                                                                                                    cGCCATGATAAGGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTGTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGAc                                                      <  2:361635/251‑1 (MQ=255)                                                                                                                                                                                        aggATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGc                                                  <  2:201165/249‑1 (MQ=255)                                                                                                                                                                                                        cATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGtact                                  <  2:363873/251‑1 (MQ=255)                                                                                                                                                                                                          tCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGtactac                                >  1:402730/1‑251 (MQ=255)                                                                                                                                                                                                                  gATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACg                        <  1:516144/251‑1 (MQ=255)                                                                                                                                                                                                                       tACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTa                    <  2:577689/250‑1 (MQ=255)                                                                                                                                                                                                                                        aTAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTTTTATTTTTAAGTTGCTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCGTGTACGATTACTGTTTGCTTTCATCAGt  <  2:551638/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    AACTGGCAGTGTCGCCAGAGAGATGTTGCC‑AGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGT  >  NC_000913/4366432‑4366911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑