Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,351,590	C→T	V25I (GTC→ATC)	dcuS ←	sensor histidine kinase DcuS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,351,590	0	C	T	100.0%	191.7 / NA	58	V25I (GTC→ATC)	dcuS	sensor histidine kinase DcuS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/37);  total (21/37)

AAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGA  >  NC_000913/4351345‑4351829                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                aaGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTa                                                                                                                                                                                                                                            <  2:390973/251‑1 (MQ=255)   gACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTa                                                                                                                                                                                                                                            >  2:435052/1‑249 (MQ=255)   aaCTTTGCATATCGGTAACGACAATAAACAGCAGAGCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCTCCGAGAACAGTACCGCACTGATCATTAAg                                                                                                                                                                                                                                          <  1:375835/250‑1 (MQ=255)    aCTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTTCCGCACTGATCATTAAGa                                                                                                                                                                                                                                         <  1:397968/251‑1 (MQ=255)       ttGCATATCGGTAACGACAATAAACAGCAGAGCGTTGCGTTTGCGGACGGCTTCCGCGATGGCCGGGATGCCACGCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGAt                                                                                                                                                                                                                                        <  1:353632/249‑1 (MQ=255)          cATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCCCTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACtg                                                                                                                                                                                                                                   >  1:479939/1‑251 (MQ=255)            tatCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACtgtg                                                                                                                                                                                                                                 >  1:492039/1‑251 (MQ=255)             atCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTAATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGTTCACtgggg                                                                                                                                                                                                                                >  2:437834/1‑248 (MQ=255)               cGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGt                                                                                                                                                                                                                               >  1:154406/1‑250 (MQ=255)               cGGTAACGACAATAAACAGCAGAGCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCGGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGt                                                                                                                                                                                                                               <  2:459152/250‑1 (MQ=255)                 gTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGCTGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACt                                                                                                                                                                                                                            >  1:266898/1‑251 (MQ=255)                        cAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCAACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACCGATCATTAAGATCACTGTGGTACTCAAttcc                                                                                                                                                                                                                     >  2:219684/1‑249 (MQ=255)                         aaTAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGCGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCACTAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTc                                                                                                                                                                                                                     >  1:375866/1‑250 (MQ=255)                             aaCAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCgg                                                                                                                                                                                                                >  2:389438/1‑251 (MQ=255)                             aaCAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCGGCGGGTTTTGCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTTGTACTCAATTTCATCgg                                                                                                                                                                                                                <  2:308719/251‑1 (MQ=255)                                         tttcgtttgcgtACGGCTTCCGCGATGGCCGTGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACAGAGAACAGTACCTCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTa                                                                                                                                                                                                     <  2:568784/247‑1 (MQ=255)                                               ttgcgtACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGc                                                                                                                                                                                               <  1:425011/250‑1 (MQ=255)                                                 gcgtACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTGCCGCACTGATCAGTAAGATCACTGTGGTACTCAATTTCATCGGCCGTTTGCGTAACATGCgg                                                                                                                                                                                             <  2:333311/250‑1 (MQ=255)                                                  cgtACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGAGTTGCGAGAAGTAAATCAGATGCGCCACCAATAGCACCGAGAACAGGACCGCACGGATCATTAAGAGCACTGTGGTACTCAATTTCATCGGAGGTGTGCGGAACATGCGGTa                                                                                                                                                                                           <  2:349193/251‑1 (MQ=255)                                                   gtACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAg                                                                                                                                                                                          <  1:441700/251‑1 (MQ=255)                                                     aCGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGACCTGTCGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCAATGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCTCCGAGAACAGTTCCGCACTGATCATTAAGATCACTGTGGTACTCATTTTCATCGGACGGTTGCGTAACATGCGGTAgg                                                                                                                                                                                         <  1:431329/250‑1 (MQ=255)                                                       ggCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCa                                                                                                                                                                                      <  1:484862/251‑1 (MQ=255)                                                           tCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCCTTAAGATCACTGTGGTACTCAATTTCATCGGACGCTTGCGTAACATGCGGTAGGGCaatg                                                                                                                                                                                   >  2:482736/1‑250 (MQ=255)                                                                 aTGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGtct                                                                                                                                                                            <  2:512945/251‑1 (MQ=255)                                                                   ggCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCtca                                                                                                                                                                          <  1:317531/251‑1 (MQ=255)                                                                        ggATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCtcatca                                                                                                                                                                       <  1:76541/249‑1 (MQ=255)                                                                         gATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCGCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCCCCAAGAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCt                                                                                                                                                                    <  1:334574/251‑1 (MQ=255)                                                                                 cccccggCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTtgtg                                                                                                                                                            <  2:361284/245‑1 (MQ=255)                                                                                     cTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGAc                                                                                                                                                        >  1:440168/1‑251 (MQ=255)                                                                                       gCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACa                                                                                                                                                       >  1:280159/1‑250 (MQ=255)                                                                                       gCGGTTTTTCCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCAGCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACa                                                                                                                                                       <  2:23963/250‑1 (MQ=255)                                                                                        cGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACaaa                                                                                                                                                     <  1:416398/251‑1 (MQ=255)                                                                                            tttttCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAAttt                                                                                                                                                  <  1:259394/250‑1 (MQ=255)                                                                                                tCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCATCTTCCTTGTGTGACAAATTTCTTa                                                                                                                                              >  2:325358/1‑250 (MQ=255)                                                                                                   gCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGc                                                                                                                                           >  1:251257/1‑250 (MQ=255)                                                                                                         ccTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTAtct                                                                                                                                    >  1:521022/1‑251 (MQ=255)                                                                                                           tGACGGATTTCCGGCGAGTCGGCGAGGGTCCGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTAtct                                                                                                                                    <  2:421133/249‑1 (MQ=255)                                                                                                               ggATTGCCGGCGAGTCGGCGAGGGTTCGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCtgatg                                                                                                                              <  2:375866/251‑1 (MQ=255)                                                                                                                         cGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTa                                                                                                                     >  1:342055/1‑250 (MQ=255)                                                                                                                            gTCGGCGAGGGTACGCGCCACTGCCAGCGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTc                                                                                                                 <  1:7693/251‑1 (MQ=255)                                                                                                                                         cgcgCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGa                                                                                                    <  2:330159/251‑1 (MQ=255)                                                                                                                                                     aGTGCATTGTCGGCTAGACCATCTCGTGTCATATCAATGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCAACGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGAAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGGAATTTAAAGGGAGTAAGaaaggttggttat                                                                                        >  2:552582/1‑240 (MQ=255)                                                                                                                                                            tgttgGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAgg                                                                                   <  1:9254/249‑1 (MQ=255)                                                                                                                                                             gttgGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGa                                                                                <  1:86467/251‑1 (MQ=255)                                                                                                                                                                       ccATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGGAATTCAAAGGGAGGAAGAATGATTGGCTATATAGGGGGAGAGActatg                                                                      >  1:490997/1‑248 (MQ=255)                                                                                                                                                                                  cATATCACTGCTTTGCGAGCAGTAAATCAGATGCACCACCAATAGCCCCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGAAATTTAAAGGGAGGAAGAAAGAATTGCTATATAGGGGAAGAGGCTCTGGGAACGGAAAc                                                           >  1:540884/1‑251 (MQ=255)                                                                                                                                                                                      tCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGc                                                                                           <  1:7335/215‑1 (MQ=255)                                                                                                                                                                                           gATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGTAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGTAAACTGCCAGt                                                    <  2:235276/249‑1 (MQ=255)                                                                                                                                                                                              ttGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTg                                                <  1:437834/250‑1 (MQ=255)                                                                                                                                                                                                                  gCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATg                           <  1:372086/251‑1 (MQ=255)                                                                                                                                                                                                                     ccaccaATAGCACCGAGAACAGTACCGCTCTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCtt                        <  2:492039/251‑1 (MQ=255)                                                                                                                                                                                                                     ccaccaATAGCACCGAGAACAGTACCGCACTGATCATGAAGAGCACTGTTGTACTCAATTTCATCGGGCGTTTGCCTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCATGTGTGACGAATGTCGTAAGCATTATCTCGGATGAGGGGGGTAAATTAAAGGGAGAAAGAATGATTGGTTATATAGGGGAAGAGACTCTGGCGACGGAAAGTGTCAGTGCTGTATGAAGATTCCGGGGCTAAGCtt                        <  2:481987/251‑1 (MQ=255)                                                                                                                                                                                                                      caccaATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGGCTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTta                       <  1:551692/251‑1 (MQ=255)                                                                                                                                                                                                                          aaTAGCACCGAGAACAGTACCGCACTGATCATTAATATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAATAATGATTTGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGc                   <  1:257733/251‑1 (MQ=255)                                                                                                                                                                                                                                            aCCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGCGATAATCATACtgatga  <  1:389438/250‑1 (MQ=255)                                                                                                                                                                                                                                            aCCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGGAATTCAAAGGGAGGAAGAATGATTGGCTATATTGGGGGAGCGACTCTGGCCACGGAAAACGCCACTGCCGTTTGTAGGTTTCGGGGCTATGCTTATAGCGATAATCCTACttatt   >  2:175411/1‑245 (MQ=255)                                                                                                                                                                                                                                                  cTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCTAAGGGGGTAAGAATGATTGGATATTTAGGGGGAGAGACTTTGGCAACGGAAACTGCCAGTGCTGTATg                                            >  1:47069/1‑202 (MQ=255)                                                                                                                                                                                                                                                  cTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATg                                            <  2:47069/202‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                AAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGA  >  NC_000913/4351345‑4351829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑