Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,342,825	C→T	N305N (AAC→AAT)	melA →	alpha‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,342,825	0	C	T	100.0%	219.2 / NA	64	N305N (AAC→AAT)	melA	alpha‑galactosidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/26);  total (38/26)

ACCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTC  >  NC_000913/4342578‑4343058                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          ccccGGAACTGTTGGCGGCTTAGGAAGCAGGGCAGGCACCTAAGCCAAATATTCAGGGCAATACTCGCGGCCAGAATATCGTGCCCTCCGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGATTACCCACCGTGTTTTATTA‑GGCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGTATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGc                                                                                                                                                                                                                                       <  2:273768/250‑1 (MQ=255) aCCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTgg                                                                                                                                                                                                                                        >  1:132424/1‑251 (MQ=255) aCCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTgg                                                                                                                                                                                                                                        <  1:287325/251‑1 (MQ=255)  cccGGAACTGCTGGCGGCTTATGAAGCATGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTTAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGTATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGc                                                                                                                                                                                                                                       <  1:503934/251‑1 (MQ=255)   ccGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGAGTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCa                                                                                                                                                                                                                                      <  1:582427/251‑1 (MQ=255)              ggcggaTTATGAAGCAGGGCAGGCCCCGAAGCCGAAAAGTCATGGCAATACTCGCTGCCAGAATATTGTGAGCGACGAAATGTTCAAAAAGCTGGGCTATTGCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGATGATTTGATTGAGCGTTAGAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTg                                                                                                                                                                                                                            <  2:30/250‑1 (MQ=255)                        gAAGCAGGGAAGGCAACGAAGCCGAATATTCATGGCAATACTCGCTCCCAGGATATTGTGCGCTACGAAATGTTCAAAAAGCTAGGATGTTTCGTAACGGCATAGTCAGAACATTTTGCTGAGTACACACCGTGGGTTATGAAGCCAGGTAGTGAGAATTTGATTGAGCGTTATAAAGAACCGCTGGATGAGTACCCGAAACGGTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGATGGAGGAGgata                                                                                                                                                                                                                  >  1:50595/1‑250 (MQ=255)                          aGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATaaa                                                                                                                                                                                                                >  1:85235/1‑250 (MQ=255)                             agggcaggCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATaaaaa                                                                                                                                                                                                              >  2:155293/1‑249 (MQ=255)                             agggcaggCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATaaaaa                                                                                                                                                                                                              >  1:281854/1‑249 (MQ=255)                                   ggcaCCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAACAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTccc                                                                                                                                                                                                      >  1:521659/1‑251 (MQ=255)                                     caCCGAAGCCTAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCgg                                                                                                                                                                                                    <  2:281854/251‑1 (MQ=255)                                         gAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGATGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGACTTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTg                                                                                                                                                                                                >  2:586216/1‑251 (MQ=255)                                               gAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTa                                                                                                                                                                                          <  1:313925/251‑1 (MQ=255)                                                 atatTCATGGCAATACTCGCTGGCAGACTCTTGTGCGATACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGCTGAGTACCCGAAACGCTGCGTCGAGAAGCTGGCGAATTGGCATAAAGAGCTGGCGGCGTCTAAAAAAGCCTCCCGGATTGATATTaa                                                                                                                                                                                         >  2:261940/1‑250 (MQ=255)                                                                           aaTATTGTGCGCTACAAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAAt                                                                                                                                                              <  2:21369/251‑1 (MQ=255)                                                                              atTGAGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACAGTGGTTTATTAAGCCCGGTCGTGAGGATTTGATTGAGCGATATAAAGTACCGCTGGATGAGTACCCGAAACGATGCGTCGAGCAGCTGGCGCATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTACCGGATTGATATTAAACCGTCAAGGGAATATGCCAGCACAATCAt                                                                                                                                                           >  1:211263/1‑251 (MQ=255)                                                                                tgtgCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATg                                                                                                                                                          >  1:50055/1‑250 (MQ=255)                                                                                      cTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCg                                                                                                                                                                                     >  1:471922/1‑217 (MQ=255)                                                                                      cTACGAAATGTTCAAAAAGCTGGGCTATTTCGGCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCg                                                                                                                                                                                     <  2:471922/217‑1 (MQ=255)                                                                                         cGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCt                                                                                                                                                >  2:390468/1‑251 (MQ=255)                                                                                           aaaTGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGATGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTgg                                                                                                                                              >  1:46757/1‑251 (MQ=255)                                                                                                ttCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTgg                                                                                                                                         >  1:76371/1‑251 (MQ=255)                                                                                                     aaaGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGc                                                                                                                                    <  2:564592/251‑1 (MQ=255)                                                                                                       aGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTGGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCg                                                                                                                                  <  2:132424/251‑1 (MQ=255)                                                                                                        gCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGTCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGATTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGa                                                                                                                                 <  1:88928/251‑1 (MQ=255)                                                                                                                aTTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGAtt                                                                                                                         >  1:136786/1‑251 (MQ=255)                                                                                                                 tttCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCGGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGCttt                                                                                                                        >  2:44386/1‑251 (MQ=255)                                                                                                                 tttCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGAttt                                                                                                                        >  1:303898/1‑251 (MQ=255)                                                                                                                  tgcGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGAGATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGAttt                                                                                                                        <  1:261940/248‑1 (MQ=255)                                                                                                                       cACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGc                                                                                                                   >  1:243021/1‑250 (MQ=255)                                                                                                                             aTCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTcc                                                                                                            <  1:510033/251‑1 (MQ=255)                                                                                                                             aTCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAAAGTcc                                                                                                            >  2:494920/1‑251 (MQ=255)                                                                                                                                 tCAGAACATTTTGCTGTGTACACGCCGTGGTTTATTAAGCCAAGTCGAGAGCGTTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTGCCCGAAACGCTGCGTCGAGCAGATGGCGCATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACTCTATCTGGATTGCAGAGCAGAGTGTGATTTCCGGCAACGTCTGTa                                                                                                         >  2:344313/1‑250 (MQ=255)                                                                                                                                        aTTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGt                                                                                                 >  2:11627/1‑251 (MQ=255)                                                                                                                                           ttGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGttt                                                                                               <  2:187522/250‑1 (MQ=255)                                                                                                                                              cTGAGTACACACCGTGGTTTATTAAGCCAGGACGAGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGCGTACCCGAAACGCTGCGTCGAGAAGCTGGCGAATTGGCATAAAGAGCTGGAGGCGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGAATGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTttgatt                                                                                           >  1:492654/1‑251 (MQ=255)                                                                                                                                                gAGTACACACCGTGGTTTATTAAGCCAGGTCGTGATGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGGCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGAGTGGTGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTttgatt                                                                                           <  2:77657/249‑1 (MQ=255)                                                                                                                                                gAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTttgattg                                                                                          >  1:393427/1‑250 (MQ=255)                                                                                                                                                   tACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGGGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATa                                                                                       >  2:429594/1‑250 (MQ=255)                                                                                                                                                           gTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACTCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGGCCGTAACGATGGTTTGATTGATAACCTGCcac                                                                              <  1:421887/251‑1 (MQ=255)                                                                                                                                                               tttATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAgg                                                                          >  1:83894/1‑251 (MQ=255)                                                                                                                                                                     aaGCCAGGTCGTGAGGATTTGATTGAGCGTTATACAGTACCGCTGGATGAGTACACGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGTGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAAACGTAACGGGAATATGCAAGCACAATAATGAACGCAATCGGGACTGGCGAGCCGAGTGGGATTTACGGCACAGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTg                                                                    >  2:539875/1‑251 (MQ=255)                                                                                                                                                                     aaGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGtt                                                                     <  1:317469/250‑1 (MQ=255)                                                                                                                                                                     aaGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTg                                                                    >  1:389845/1‑251 (MQ=255)                                                                                                                                                                      aGCCAGGACGCGAGGATTTGATTGAGCGTTATAAAGTACTGCAGGATGAGAAACAGAACCGCTGCGTCGGGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCAAGCACAATCATGAAAGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGAAGTTGc                                                                   >  2:392049/1‑251 (MQ=255)                                                                                                                                                                                tGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGt                                                           >  1:552728/1‑249 (MQ=255)                                                                                                                                                                                         gattgaGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTgg                                                <  2:157966/251‑1 (MQ=255)                                                                                                                                                                                               gCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATg                                          <  1:429594/251‑1 (MQ=255)                                                                                                                                                                                                          tACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCAtt                               >  1:467986/1‑251 (MQ=255)                                                                                                                                                                                                            ccGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCa                             <  1:118264/251‑1 (MQ=255)                                                                                                                                                                                                            ccGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCa                             <  1:177752/251‑1 (MQ=255)                                                                                                                                                                                                             cGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAACAAAGCCTCCCGGCTTGATATTAAACCGTCACGGGAATATGACAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGCTTGATAACCTGCCACACGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTc                              >  2:257287/1‑249 (MQ=255)                                                                                                                                                                                                                  gATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGAc                       <  1:12013/251‑1 (MQ=255)                                                                                                                                                                                                                       gTACCCGAAACGCTGCGTCGGGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGCGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCac                    >  1:553183/1‑248 (MQ=255)                                                                                                                                                                                                                       gTACCCGAAACGCTGCGTCGAGCAGCGGGCGAATGGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGAACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAg                  <  2:166527/251‑1 (MQ=255)                                                                                                                                                                                                                          cccGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCg               >  1:277675/1‑251 (MQ=255)                                                                                                                                                                                                                              aaaCGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGCGTATAAAAAAGCCTCCCGGATTGATATTCAACCGTCACGGGAATATGCCAGCACAATCATGAACGCAATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTACTGGCATCAAGCCGACCAAAGTCGGTAc           >  1:537900/1‑251 (MQ=255)                                                                                                                                                                                                                              aaaCGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTttgattga                                                                                         >  1:437094/1‑173 (MQ=255)                                                                                                                                                                                                                              aaaCGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTttgattga                                                                                         <  2:437094/173‑1 (MQ=255)                                                                                                                                                                                                                                aCGCTGCGTCGAGCAGATGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGc         >  1:180316/1‑251 (MQ=255)                                                                                                                                                                                                                                    ggCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCGGGACTGGCGAGCCGAGTGTGAGTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTAcc     <  1:539875/250‑1 (MQ=255)                                                                                                                                                                                                                                       gTCGAGCCGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTc  >  1:458292/1‑251 (MQ=255)                                                                                                                                                                                                                                       gTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTc  >  2:459001/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          ACCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTCAGCCGACCAAAGTCGGTACGCTACCTTC  >  NC_000913/4342578‑4343058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑