Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,325,644	C→T	S33N (AGC→AAC)	yjdN ←	PF06983 family protein YjdN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,325,644	0	C	T	100.0%	212.4 / NA	64	S33N (AGC→AAC)	yjdN	PF06983 family protein YjdN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/27);  total (37/27)

GCCATTTCGATTTTTCCGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGCTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGA  >  NC_000913/4325398‑4325887                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    gCCATTTCGATTTTTCCGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGAt                                                                                                                                                                                                                                                 <  1:148416/251‑1 (MQ=255)     tttCGATTTTTCCGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGAtttt                                                                                                                                                                                                                                              >  1:74135/1‑250 (MQ=255)       tCGATTTTTCCGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTtat                                                                                                                                                                                                                                            <  1:434598/250‑1 (MQ=255)              ttCCGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAg                                                                                                                                                                                                                                     <  1:562234/250‑1 (MQ=255)               tCCGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGt                                                                                                                                                                                                                                    <  1:414994/250‑1 (MQ=255)                  gTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCcg                                                                                                                                                                                                                                >  1:477481/1‑251 (MQ=255)                         gCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCaa                                                                                                                                                                                                                         <  2:451433/251‑1 (MQ=255)                          cAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAac                                                                                                                                                                                                                        >  1:536680/1‑251 (MQ=255)                               ttGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCCGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAacgtacg                                                                                                                                                                                                                   >  2:104323/1‑251 (MQ=255)                                   cAAACCAGCGTTTTCCTTCTTCGACCTGTGTCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCGGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTg                                                                                                                                                                                                                <  2:342826/250‑1 (MQ=255)                                    aaaCCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGCTGTCGCTTCCGGCAATGCGCAAATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCACCGTACGTTGat                                                                                                                                                                                                              >  2:284401/1‑251 (MQ=255)                                     aaCCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGCTTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGat                                                                                                                                                                                                              >  2:286131/1‑250 (MQ=255)                                         aGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGataat                                                                                                                                                                                                           >  1:130198/1‑249 (MQ=255)                                              tttCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAAt                                                                                                                                                                                                    >  2:215304/1‑251 (MQ=255)                                                    tcttcGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTc                                                                                                                                                                                              >  1:82924/1‑251 (MQ=255)                                                               ttGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGGCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCTGAACAGTTa                                                                                                                                                                                    <  2:394594/250‑1 (MQ=255)                                                                  cGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCgg                                                                                                                                                                                >  1:146493/1‑251 (MQ=255)                                                                    aaTCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATTGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCTCTTTCCTGCGCTGATTTTGGCAGTTCTCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCa                                                                                                                                                                              <  1:391086/251‑1 (MQ=255)                                                                       cGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGACCAGTTACCGGCaaa                                                                                                                                                                            >  2:458778/1‑250 (MQ=255)                                                                            acaaGCGTAAAGACGGAGTAGCAGGCTTCCCCTGAAGGAAAGGCATCGCTCATCATGATGTAGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAACTTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCATGCGATGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCAGCGCCCAACGTACGTTGATAATAGGCAATCGAGTCGGAACAGTTACCGGCAACagag                                                                                                                                                                        >  2:497147/4‑249 (MQ=255)                                                                              cAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGt                                                                                                                                                                    <  1:250229/251‑1 (MQ=255)                                                                                  gTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAggg                                                                                                                                                                >  1:272690/1‑251 (MQ=255)                                                                                       gCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACtt                                                                                                                                                            >  1:164896/1‑250 (MQ=255)                                                                                           gAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTCTCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCCACGTACGTTGATAATAGGCAATCGCGTCGGAACAGGTACCGGCCACAGAGCGGTAGGGACATAACgg                                                                                                                                                       >  2:418060/1‑251 (MQ=255)                                                                                            aGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGTGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGCGAGGTAGGGAGTTAACgac                                                                                                                                                      >  2:435709/1‑249 (MQ=255)                                                                                                       tttCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTg                                                                                                                                             <  2:45481/249‑1 (MQ=255)                                                                                                          ccTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGGAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTc                                                                                                                                        >  1:159327/1‑251 (MQ=255)                                                                                                          ccTGACGGAATGGCAGCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCt                                                                                                                                         <  2:24120/250‑1 (MQ=255)                                                                                                                    tggcatCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGCAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGAATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGACCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTaaa                                                                                                                                 >  1:537758/1‑247 (MQ=255)                                                                                                                              catcatGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGtt                                                                                                                      <  1:182166/249‑1 (MQ=255)                                                                                                                                 catGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAgtg                                                                                                                 <  1:418060/251‑1 (MQ=255)                                                                                                                                          gCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGaa                                                                                                        <  2:495506/251‑1 (MQ=255)                                                                                                                                               cGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGCAGTTGATTTTATAGAGCAGTTCCGCGACCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAAAGGCATGATGGTGACCTCAGTTAAGAGAAGCCCGTTAAGTGTAGTTCGACAttt                                                                                                    >  2:551056/1‑250 (MQ=255)                                                                                                                                                           caTTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGCCTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACCGATgag                                                                                         >  2:550317/1‑249 (MQ=255)                                                                                                                                                                  gTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCt                                                                                <  2:528415/251‑1 (MQ=255)                                                                                                                                                                  gTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCGGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTGCGAAATTTATTCAGATGAGAGGCGGCCt                                                                                <  2:323228/251‑1 (MQ=255)                                                                                                                                                                    gggCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCatgatg                                                                                                                                                >  1:260961/1‑185 (MQ=255)                                                                                                                                                                    gggCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCatgatg                                                                                                                                                <  2:260961/185‑1 (MQ=255)                                                                                                                                                                         aTGGCGGTATCGGGAAATGGCATTCCGTAAGGGCAGTTCTCGGCGCTGTCCGGCGCTGATTGTGGCATTGCGCCGAAGTTGATTTTAGAGAGCAGTTCCGCGCCCAACGTACGTTGATAGTAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTGAGCGGCATGATGGTGACCTCAGTCAAGAGATGCCAGTGAAGTGGAGTTCGAAATTTATACAGATGAGATGCGGCCGGTTAAGa                                                                         <  2:565743/251‑1 (MQ=255)                                                                                                                                                                           ggCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGAc                                                                        <  2:488167/250‑1 (MQ=255)                                                                                                                                                                               gTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTa                                                                                                                     >  2:130337/1‑201 (MQ=255)                                                                                                                                                                               gTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTa                                                                                                                     <  1:130337/201‑1 (MQ=255)                                                                                                                                                                               gTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGAcgcgcg                                                                   >  2:98366/1‑251 (MQ=255)                                                                                                                                                                                tATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGAcgcgcgg                                                                  >  2:223446/1‑250 (MQ=255)                                                                                                                                                                                           tGCATTCCGGACTGTCAGTTCTCGGCGCTGCCCTGCGCTGATTTCGGCATTTCTCCGAAGTTTATTTTATAGAGCAGTTCCGCGCCCTACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATGCAGATGAGAGGCGGCCTGATAAGACGCGCTAGCGCCGCATc                                                        <  2:328949/250‑1 (MQ=255)                                                                                                                                                                                            gCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGCGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGCGGTAGGGACTTAACGGCATGATGGTGACCTCCGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAggcggc                                                                                  >  2:380517/1‑223 (MQ=255)                                                                                                                                                                                            gCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAggcggc                                                                                  <  1:380517/223‑1 (MQ=255)                                                                                                                                                                                             cATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATccgg                                                     >  2:398433/1‑248 (MQ=255)                                                                                                                                                                                                  cGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGTAGTc                                                >  2:194248/1‑251 (MQ=255)                                                                                                                                                                                                  cGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGGTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGTAGTc                                                <  2:477481/251‑1 (MQ=255)                                                                                                                                                                                                     aaGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCCTCAGGTAGTCGGc                                             >  2:146262/1‑251 (MQ=255)                                                                                                                                                                                                     aaGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGTAGTCGGc                                             >  2:3520/1‑251 (MQ=255)                                                                                                                                                                                                      aGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGTAGTCGGc                                             >  1:197189/1‑250 (MQ=255)                                                                                                                                                                                                                  ggCGCTGTCCTGCGCTGACTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCAAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGAATCAGGTAGTCGGCACTGTTGCCGGAt                                >  2:361306/1‑251 (MQ=255)                                                                                                                                                                                                                     gcggTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGTAGTCGGCACTGTTGCCGGATgcg                             <  1:458778/248‑1 (MQ=255)                                                                                                                                                                                                                           cTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTAACGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGTAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGTAGACGGCACTGTTGCCGGATGCGGCGTa                        >  1:230012/1‑250 (MQ=255)                                                                                                                                                                                                                                 tGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGTAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCt                 >  1:460774/1‑251 (MQ=255)                                                                                                                                                                                                                                        ggCATTTCGCCGAAGTTGATTTTATAGAGCAGTTACGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGTAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCg           <  2:146593/250‑1 (MQ=255)                                                                                                                                                                                                                                        ggCATTGCGCCGAAGTTGATTTTATAGAGCAGTCCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGGCGTAACAGTTACCGTCAAAAGAGATGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGTAGTCGTCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCgg          <  1:255186/251‑1 (MQ=38)                                                                                                                                                                                                                                           aTTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGTAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt       >  1:277691/1‑251 (MQ=255)                                                                                                                                                                                                                                           aTTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGAGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGTAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCggact       >  1:498638/1‑248 (MQ=255)                                                                                                                                                                                                                                              tCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGTAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACg    >  1:435188/1‑251 (MQ=255)                                                                                                                                                                                                                                                gCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGTAGTCGGCACTGTTGCCGGCTGCGGCGTAAACGCCTTCTCCGGCCTACgg   >  2:539163/1‑250 (MQ=255)                                                                                                                                                                                                                                                gCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGTAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGa  <  1:281504/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    GCCATTTCGATTTTTCCGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGCTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGA  >  NC_000913/4325398‑4325887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑