Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,297,469	C→T	G633D (GGC→GAC)	fdhF ←	formate dehydrogenase H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,297,469	0	C	T	100.0%	237.4 / NA	71	G633D (GGC→GAC)	fdhF	formate dehydrogenase H
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/30);  total (41/30)

CTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGCCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACC  >  NC_000913/4297233‑4297710                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    cTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGAACTACTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTCTTTGTACTCCGGCGGTTTAGTAATCGGGCTTAAGTTTTAGGTAACCCGCTCGTTCAAGGCACCAATCCACCACTGGTAGGTCTTGTAAATCGCCCCTTTGTTCGGACCATCGCTGACCTGCGAACGGGTGATAAATTTATCTTTACTCGAGTGc                                                                                                                                                                                                                                       >  1:581072/1‑251 (MQ=255)   tCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCa                                                                                                                                                                                                                                      <  2:382145/250‑1 (MQ=255)       gcAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCaa                                                                                                                                                                                                                                 <  2:248594/251‑1 (MQ=255)             gAGTTTTAAACTTGTTGTACTCGTCAATCACGTACCGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGCACTCCGGCGCTTTCGTAATAGGGCTTAGGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATAGCCCCTTTGTTCGGACGATCGCTGACCCGCGCCCGGGTGATCATTTTGTCTTTACGCGAGTGCACCCAAAcccct                                                                                                                                                                                                                           >  2:352793/1‑248 (MQ=255)                ttttCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCTCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTCAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAAGGcc                                                                                                                                                                                                                        >  2:83616/1‑251 (MQ=255)                    cAACTTGTTGTACTCGTCAATCACGTTCTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAt                                                                                                                                                                                                                    <  1:356288/251‑1 (MQ=255)                    cAACTTGTTGTACTCGTCAATAGCGTACTGCTCGGCGGCGCGCTGATCGGCTATCGGCTCGACGCGAACGGCGCAGTATTTGTTCTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCCAACCAATGCCTCa                                                                                                                                                                                                                     <  2:314895/250‑1 (MQ=255)                    cAACGTGTTGTGCTCGTCAATCACGTGCTGCTCGTCTGCGCGCTGATCGGCGCTCGGCTCGACTCGAACGGCGCAGTATTTGTACTCCGTCGTTTTCGTAATCGGGCTTAAGTTGTCGGTAACCAGCTCGTTACAGGCACCAATCCGCCACTGGTAGGTCATGTAAATCGCCCTTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAt                                                                                                                                                                                                                    <  1:294082/251‑1 (MQ=255)                         tgttgtACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑Ctt                                                                                                                                                                                                                <  1:145644/249‑1 (MQ=255)                         tgttgtACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTc                                                                                                                                                                                                               >  1:124166/1‑250 (MQ=255)                              tACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCCAACCAATGCCTCAT‑CTTCAATAcc                                                                                                                                                                                                         >  2:198173/1‑251 (MQ=255)                               gcgcgtgaaacACGTACTGCTAGGCGGCGCGCGGATCGGCTATCGGCGATACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGCAATCGGGCTTAAGTTGTCGGTAACCAGCTCGTGACAGGCACCAATCCTCCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGGCGAGCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAATCCAATGCCTCAG‑CTTCAATAccc                                                                                                                                                                                                        <  2:235352/241‑1 (MQ=255)                                         cACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTg                                                                                                                                                                                              <  1:197118/250‑1 (MQ=255)                                          aCGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGc                                                                                                                                                                                            >  1:312237/1‑251 (MQ=255)                                           cGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTCCAGGCACCAATCCACCCCTGGTGGGTAATGTAAATCCCCCCTTTGTTCGGCAGATCGCTGACCTGCGCCCGGGTGATAATTTTTTCTTTACGCGGGGGACCCCAAACCAATGCC‑CATTCTCCAATACC‑AGACGGTTTGGCg                                                                                                                                                                                           >  1:394672/1‑251 (MQ=255)                                              aCTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTc                                                                                                                                                                                         <  1:142992/250‑1 (MQ=255)                                              aCTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCt                                                                                                                                                                                        <  1:557557/251‑1 (MQ=255)                                                 gCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCg                                                                                                                                                                                     <  1:376245/251‑1 (MQ=255)                                                     ggcggcGCGCTGATCGGCGATCGGCTAGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGATTGCCCCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTCGCGTCGTCGGTAt                                                                                                                                                                                 <  2:91547/251‑1 (MQ=255)                                                     ggcggaGCGCTGATCGGCGATCGGATCGACGCGAACGGCGCAGTCTTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCCCCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGCTCGCTGACCTGCGCCCGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTAt                                                                                                                                                                                 >  2:492086/1‑251 (MQ=255)                                                        ggcgCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGCCCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCCT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGGATTg                                                                                                                                                                               >  2:109165/1‑250 (MQ=255)                                                        ggcgCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCAccc                                                                                                                                                                                                                                   <  1:298732/200‑1 (MQ=255)                                                        ggcgCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCAccc                                                                                                                                                                                                                                   >  2:298732/1‑200 (MQ=255)                                                        ggcgCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGCCG‑TTTGGCGTCTTCGGTATTg                                                                                                                                                                               >  2:472150/1‑250 (MQ=255)                                                          cgcgcTGATCGGCGAGCGGGTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCTGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCTTTTGTTCGGACGATCGGTGTGCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGGGCATCCAATGCAATTGCTCAG‑CTTCGAGACCCAGACG‑TTTGGCGTCTGCGGTAGTGAtt                                                                                                                                                                            <  2:79974/251‑1 (MQ=255)                                                            cgcTGATCGGCGATCGGCTCGACGAGAACGGCGCAGTATTTGTAATCCGGCGTATTCGTCATCGGGCTTAAGTTTTCGGAAACCAGCTAGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATAGCTGTCCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTtg                                                                                                                                                                          >  2:575069/1‑251 (MQ=255)                                                                   cgtcgatcggcTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTGTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTa                                                                                                                                                                    <  2:76081/247‑1 (MQ=255)                                                                   cggcgatcggcTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGt                                                                                                                                                                     >  1:463989/1‑249 (MQ=255)                                                                   cggcgatcggcTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAg                                                                                                                                                                   <  2:1448/251‑1 (MQ=255)                                                                    ggcgatcggcTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGc                                                                                                                                                                  >  2:280996/1‑251 (MQ=255)                                                                          cggcTAGACGCGACCGGCGCAGTATTTGTACTACGGCGGTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGAGTAGCCAGGtt                                                                                                                                                            >  2:496121/1‑251 (MQ=255)                                                                                gACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTtcatc                                                                                                                                                        >  1:96194/1‑249 (MQ=255)                                                                                         ggCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGcgccg                                                                                                                                             >  2:14997/1‑251 (MQ=255)                                                                                           cgcAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCgccgcc                                                                                                                                           >  2:147488/1‑251 (MQ=255)                                                                                           cgcAGTATTTGTACTCCGGCGTGTTCGTAATCGGGCTTAAGTTTTCGGGAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGGGCGTAGCCAGGTTCATCAGCCAGCgccgcc                                                                                                                                           <  2:6926/251‑1 (MQ=255)                                                                                             cAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGCTTTGTGCGTAGCCAGGTTCATCAGCCAGCgccgcc                                                                                                                                           >  2:570620/1‑249 (MQ=255)                                                                                                             gCGTTTGCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCCCCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGGGCACCCAAACAAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGGGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCg                                                                                                                         <  2:463989/251‑1 (MQ=255)                                                                                                                      tAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGa                                                                                                                 >  2:438641/1‑250 (MQ=255)                                                                                                                          cGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGc                                                                                                            >  2:509879/1‑251 (MQ=255)                                                                                                                               ttAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAag                                                                                                         >  2:175648/1‑249 (MQ=255)                                                                                                                                tAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCGGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAagag                                                                                                       <  1:388530/250‑1 (MQ=255)                                                                                                                                 aagttgtgggTAACCAGCTCGTTACAGGCACAAAACCACCGCTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCTCTGACCTGCGCCCGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCTTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTTCTTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTTCCGGTTATTTAACGGCAAGagta                                                                                                     <  2:245159/243‑1 (MQ=255)                                                                                                                                        cGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGAcc                                                                                               >  2:175918/1‑250 (MQ=255)                                                                                                                                               cAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACATGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCAc                                                                                       >  1:554383/1‑251 (MQ=255)                                                                                                                                                aGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGagt                                                                                                      >  1:390290/1‑235 (MQ=255)                                                                                                                                                aGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAAGGCCTCAT‑CTTCAATCCCCAGACG‑TTTGGCGTCTTCGGTATTGATGTGTGCGTACCCAGGTCCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGagt                                                                                                      <  2:390290/235‑1 (MQ=255)                                                                                                                                                 cctctttACAGGCTCCAACCCACCACTGGTCGGGTATGTAATTCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTTTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGATATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGc                                                                                     <  2:331140/246‑1 (MQ=255)                                                                                                                                                  cTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGTACGGCAAGAGTAGTGACCAACTTCACGc                                                                                     >  1:98585/1‑250 (MQ=255)                                                                                                                                                     gTTACAGGCACCAATCCCCCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAAGACCCAGACG‑TTTGGCGTCTCCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCg                                                                                 <  1:472150/251‑1 (MQ=255)                                                                                                                                                            gCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGAc                                                                            >  1:47371/1‑249 (MQ=255)                                                                                                                                                                 aaTCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTAcc                                                                      >  2:355867/1‑250 (MQ=255)                                                                                                                                                                   tCCACCCCTGGTAGGTCATGTAAATAGCCCCTTTGTTCGGACGATCACTGACTTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAACCCACTGCCTCAT‑CTTCAATACCCAGCCG‑TTTGGCGTCTTAGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCCTAGAACGGCGAGAGTACTGACCAACTTCACGCACCGTTGACAGTACCATc                                                                   >  2:72512/1‑251 (MQ=255)                                                                                                                                                                         acTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTAc                                                             <  1:280996/251‑1 (MQ=255)                                                                                                                                                                           tGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCCAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACtc                                                           >  2:287725/1‑251 (MQ=255)                                                                                                                                                                           tGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTCTTGATTTGTGCGTAGCAAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACtc                                                           >  2:287714/1‑251 (MQ=255)                                                                                                                                                                             gTAGGTCATGTAAATCGCCCCTTTGTCCGGACGATCGCTGACCTGCGCACGGGGGATAATTTTGTCTTTCCGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATCCCCAGACG‑TTTGGCGTCTGCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACtcgt                                                         <  2:312237/251‑1 (MQ=255)                                                                                                                                                                                    aggTAAATCTCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATTCCTCCT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAg                                                  <  2:446876/249‑1 (MQ=255)                                                                                                                                                                                      gTAAATCGCCCCTTTGTTCGGACGATCGCGGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCGCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTGGACAGTACCATCGGGTACTCGTCGGTGAGtt                                                <  1:492086/251‑1 (MQ=255)                                                                                                                                                                                         aaTCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTg                                                    >  2:289960/1‑244 (MQ=255)                                                                                                                                                                                         aaTCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTg                                                    <  1:289960/244‑1 (MQ=255)                                                                                                                                                                                             gCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGAt                                         >  2:34801/1‑251 (MQ=255)                                                                                                                                                                                                   ttGTTCGGACGATCGCTGACATGCGAACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCACTGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCCCAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGCTTGgctg                                   >  2:587172/1‑249 (MQ=255)                                                                                                                                                                                                        cGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTAccc                              >  1:117178/1‑251 (MQ=255)                                                                                                                                                                                                          gACGATCGCTGACCTGCGCACGGGTGATAATTTTTTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCAg                            <  1:198173/251‑1 (MQ=255)                                                                                                                                                                                                             gATCGCTGACCTGCGCACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCCCGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTAGATTGGCGCTACCCAGTCg                         >  2:330437/1‑251 (MQ=255)                                                                                                                                                                                                                           gcACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCAGTCGCAGGTGAAGAACTg           >  1:485514/1‑251 (MQ=255)                                                                                                                                                                                                                            cACGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGCGTTTGTCGATTGGCGCTACCCAGTCGCAGGTGAAGAACTgc          >  1:406903/1‑251 (MQ=255)                                                                                                                                                                                                                              cGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCAGTCGCAGGTg                   <  1:240085/240‑1 (MQ=255)                                                                                                                                                                                                                              cGGGTGATAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCAGTCGCAGGTg                   >  2:240085/1‑240 (MQ=255)                                                                                                                                                                                                                               gggTGATAATTTTGTCTTTACGCGAGTGCACCCAACCCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGCCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCCGTCGCAGGTGAAGAACTGCGcc       >  2:71327/1‑251 (MQ=255)                                                                                                                                                                                                                                    aTAATTTTGTCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTATGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGAcc  >  1:309596/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    CTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCGTTTTCGTAATCGGGCTTAAGTTTTCGGTAACCAGCTCGTTACAGGCACCAATCCACCACTGGTAGGTCATGTAAATCGCCCCTTTGTTCGGACGATCGCTGACCTGCGCACGGGTGATAATTTTGCCTTTACGCGAGTGCACCCAAACCAATGCCTCAT‑CTTCAATACCCAGACG‑TTTGGCGTCTTCGGTATTGATTTGTGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACC  >  NC_000913/4297233‑4297710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑