Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,284,077	C→T	A276T (GCG→ACG)	actP ←	acetate/glycolate:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,284,077	0	C	T	98.9%	288.3 / NA	87	A276T (GCG→ACG)	actP	acetate/glycolate:cation symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (50/36);  minor base G (0/1);  total (50/37)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGT‑GAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTACATCATC  >  NC_000913/4283831‑4284327                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                             cGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGT‑GAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCcgtcgt                                                                                                                                                                                                                                                           >  2:270113/1‑250 (MQ=255) cGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGT‑GAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCcgtcgt                                                                                                                                                                                                                                                           >  1:20906/1‑250 (MQ=255)      aaCAGGTTGCCGCCCACTGCATTCGCCAGGT‑GAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCaaa                                                                                                                                                                                                                                                     <  1:252070/251‑1 (MQ=255)        cAGGTTGCCGCCCACTGCATTCGCCAGGT‑GAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAAc                                                                                                                                                                                                                                                    >  2:303191/1‑250 (MQ=255)               ccgccCCCTGCATGCGC‑AGGTTGAACGGCCGCTATTTTGTTACCACCAATCAGATGGCCCGCCGCGTCGTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGGCAGAATATAGAAGTAGCCCATAAACCCGGGGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGGATGTGCTGCAAGCCCGTCGCACCAAAAATCAG‑Tcc                                                                                                                                                                                                                                           <  2:374556/251‑1 (MQ=255)                 gccCACTGCATTCGCCAGGT‑GAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGTTTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGACAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGCCTGTAAAGAAGCGCATCAGAATTTGCGGCAAGCCCGTCGTACCAAACAACAG‑Tccc                                                                                                                                                                                                                                          >  2:521453/1‑250 (MQ=255)                              gCCAGGT‑GAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCagagag                                                                                                                                                                                                                              >  1:233697/1‑249 (MQ=255)                               ccAGGT‑GAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAgc                                                                                                                                                                                                                           >  2:500385/1‑251 (MQ=255)                                cAGGT‑GAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑CCCCAGACCCAGAGAAAgcg                                                                                                                                                                                                                          >  2:330524/1‑251 (MQ=255)                                 aGGT‑GAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAgcgc                                                                                                                                                                                                                         <  1:148552/251‑1 (MQ=255)                                 aGGT‑GAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCAGCGTCTTCATATTCAGGATTCGAACCAACCAGCATGATCGCACCGAAGCCGATAATAAAGGACCGAATATAGAAGTAGCCAATAAACACGGTGGCGAAGCACAAGCTCTTAAGGGCTTCGCGGACATC‑GCTGACTGTAAAGAAGCGCATAAGAGTGTGCGGCAAGCACGTCGAACCAAACATAAG‑TCCCAGACCCAGAGAGAgcgc                                                                                                                                                                                                                         >  1:281997/1‑251 (MQ=255)                                      gAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCgg                                                                                                                                                                                                                       >  1:20759/1‑249 (MQ=255)                                      gAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCgg                                                                                                                                                                                                                       >  1:580655/1‑249 (MQ=255)                                       aaCGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGgag‑a                                                                                                                                                                                                                   <  1:500385/251‑1 (MQ=255)                                            ccgccATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCgg                                                                                                                                                                                                                       >  2:412445/1‑243 (MQ=255)                                             cgccATGTTGTTACCACCACTCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGAt                                                                                                                                                                                                             >  1:165375/1‑251 (MQ=255)                                              gccATGTTGTTACCACCACTCAACTGGCCCGCCGCGTCTTTATATTCCGGCTTCGCACCAACCAGCATGATCGCGCCGACGCCGATAATCAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATc                                                                                                                                                                                                            >  2:251062/1‑251 (MQ=255)                                                                aTCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACC‑AGAGAGGGCGCGGAGGACCGG‑TCTTTCACCCGCCCCCCCGGc                                                                                                                                                                                         >  1:110746/1‑251 (MQ=255)                                                                       ggCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCATAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCatg                                                                                                                                                                                   <  2:492390/251‑1 (MQ=255)                                                                         cccGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGCATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCatgat                                                                                                                                                                                 >  1:503520/1‑251 (MQ=255)                                                                          ccgccgCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTCAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCatgatg                                                                                                                                                                                >  1:404469/1‑251 (MQ=255)                                                                           cgccgcGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGt                                                                                                                                                                               >  1:181498/1‑251 (MQ=255)                                                                            gccgcGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTc                                                                                                                                                                              <  1:164469/251‑1 (MQ=255)                                                                             ccgcGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCAATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATCATGTcc                                                                                                                                                                             >  1:60147/1‑250 (MQ=255)                                                                                 gTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGATCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGacac                                                                                                                                                                         >  2:285643/1‑251 (MQ=255)                                                                                  tCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGCTGTCGACAcc                                                                                                                                                                        >  1:196764/1‑251 (MQ=255)                                                                                      tataTTCCGGATTCGCACCAACCCGCATGATCGCGCCGAAGCCGATAACAAAGGTCAGAATATAGAACTAGCCCACAAAACCGGCAGCGTAGCACACGCTCCTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTc                                                                                                                                                                    >  1:35621/1‑251 (MQ=255)                                                                                       atatTCGGGATTCGAACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGCCAGAATATAGAGGTAGCCCATAAACCCGGTGGAGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TACCAGACACAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCAGCCCGACTTCATGATGTCGACACCTTTCg                                                                                                                                                                   >  2:234335/1‑251 (MQ=255)                                                                                            ccGGATTCGCACCAACCAGCCTGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGgtgt                                                                                                                                                              >  1:444047/1‑251 (MQ=255)                                                                                             cGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGgtgt                                                                                                                                                              >  1:276525/1‑250 (MQ=255)                                                                                                   cGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGcc                                                                                                                                                        >  1:227175/1‑250 (MQ=255)                                                                                                   cGCACCAACCAGCATGACCGCGCCGAAGCCGAGAATAAAGGTCAGAATATAGAAGTAGCCCATAAGCCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGcc                                                                                                                                                        <  2:428003/250‑1 (MQ=255)                                                                                                     cACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGCGTGTCCCGCCATc                                                                                                                                                     <  1:330524/251‑1 (MQ=255)                                                                                                     cACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCGTCGCGGGCATC‑GCGGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGTCCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATc                                                                                                                                                     <  1:547865/251‑1 (MQ=255)                                                                                                         aaccaGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCtt                                                                                                                                                 <  1:132306/251‑1 (MQ=255)                                                                                                           ccaGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGTCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCCTCGCTTCa                                                                                                                                               <  2:138097/251‑1 (MQ=255)                                                                                                             aGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACt                                                                                                                                             <  2:109780/251‑1 (MQ=255)                                                                                                             aGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACt                                                                                                                                             <  1:134584/251‑1 (MQ=255)                                                                                                               cATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGa                                                                                                                                           <  1:72866/251‑1 (MQ=255)                                                                                                                         ccGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGAttgtt                                                                                                                                 <  1:198371/251‑1 (MQ=255)                                                                                                                               ccGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCt                                                                                                                           >  2:132027/1‑251 (MQ=255)                                                                                                                               ccGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TACCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCt                                                                                                                           >  1:393750/1‑251 (MQ=255)                                                                                                                                cGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGCATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGACATCGCTTCACTGAACAGATTGTTGAAGCt                                                                                                                           >  1:389059/1‑250 (MQ=255)                                                                                                                                 gATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTaa                                                                                                                         <  1:412445/251‑1 (MQ=255)                                                                                                                                  ataataAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGGCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTaaa                                                                                                                        <  2:165375/251‑1 (MQ=255)                                                                                                                                       aaaGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTAcc                                                                                                                                                           >  1:177561/1‑211 (MQ=255)                                                                                                                                       aaaGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTAcc                                                                                                                                                           <  2:177561/211‑1 (MQ=255)                                                                                                                                         aGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACg                                                                                                                 >  1:278150/1‑251 (MQ=255)                                                                                                                                             cAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGtt                                                                                                             >  1:406841/1‑251 (MQ=255)                                                                                                                                               gAATAGAGAAGTAGCCCATAAACCCGGTGGCGTAGATCACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCGCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTtc                                                                                                           <  2:44087/251‑1 (MQ=255)                                                                                                                                                   ataGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTtcatc                                                                                                        >  1:8552/1‑250 (MQ=255)                                                                                                                                                   ataGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCAGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTtcatca                                                                                                       <  1:18380/251‑1 (MQ=255)                                                                                                                                                    taGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCAc                                                                                                      >  1:417319/1‑251 (MQ=255)                                                                                                                                                      gAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCa                                                                                                    <  1:74892/251‑1 (MQ=255)                                                                                                                                                         gTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATaa                                                                                                 <  1:521453/251‑1 (MQ=255)                                                                                                                                                         gTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGAACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATa                                                                                                  >  1:114304/1‑250 (MQ=255)                                                                                                                                                           ggCCCATAACCCCGGGGGCGTGGAACCCGCCCTTAGGGGCTT‑GGGGGGATCCGCTGACGGAAAAGAAGGGCACCAGAAGGTGCGGCAAGCCCGTCGTCCCAACCA‑CAGGCCCCAGACC‑AGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCAGGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAgg                                                                                              <  2:110746/250‑1 (MQ=255)                                                                                                                                                           aGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAg                                                                                               <  2:8552/251‑1 (MQ=255)                                                                                                                                                           aGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAg                                                                                               <  2:528534/251‑1 (MQ=255)                                                                                                                                                            gCCCATAAACCAGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGACCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGCTTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAgg                                                                                              >  2:516792/1‑251 (MQ=255)                                                                                                                                                                  aaaCCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATa                                                                                         >  1:234969/1‑250 (MQ=255)                                                                                                                                                                    aCCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCTACGTGTGTCATCACCATAAAGGCCATAAAg                                                                                      <  1:167783/251‑1 (MQ=255)                                                                                                                                                                     cccGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGCTGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGCGTTTCATCACCATAAAGGCCATAAAGc                                                                                     >  2:136980/1‑251 (MQ=255)                                                                                                                                                                          tGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGCCGTGTTTCATCACCATAAAGGCCATAAAGCTGGc                                                                                 >  2:137108/1‑250 (MQ=255)                                                                                                                                                                              gTAGAACCCGCCCTTCCGTGCTTCG‑GGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGaa                                                                           <  2:99690/251‑1 (MQ=255)                                                                                                                                                                                     acGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGaacagca                                                                      >  1:118655/1‑250 (MQ=255)                                                                                                                                                                                          ctTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGaacagcaacagc                                                                 >  2:421308/1‑250 (MQ=255)                                                                                                                                                                                          ctTACGTGCTTCGCGGGCATC‑GCGGACTGTAAAGAAGCGCATCAGAAGGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑AGCGGATCTTCCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTGCATCACCATAAAGGCCATAAAGCTGGCACCGaacagcaacag                                                                  <  2:422646/249‑1 (MQ=255)                                                                                                                                                                                           ttACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCAc                                                               >  1:504199/1‑251 (MQ=255)                                                                                                                                                                                            tACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGGGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTGGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCAc                                                               <  2:233697/250‑1 (MQ=255)                                                                                                                                                                                               gTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCt                                                           >  1:108766/1‑251 (MQ=255)                                                                                                                                                                                               gTGCTACGCGGGCCTC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTCCCAAACATCAG‑TCCCAGACCCAGAGTGAGCGCGGAG‑ATCGGATATTTCAACAGCCCGCCCGGCTTCATGATGTAGAAACGTTTCGGGTGTACCGCAATCTCTTCACTGAACAGATTGTTGAAGCTAAAGCAGACGAGTTTCATCACCCTAAAGGCCATAAAGCTGGCACAGAGCAGCAACAGCACGGCt                                                           >  2:564940/1‑251 (MQ=255)                                                                                                                                                                                                tGCTTCGCGGGCATC‑GCTGAATGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCACGATGTCGACACCATTAGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAACACTGGCACCGAACAGCAACAGCACGGCtt                                                          >  2:587508/1‑251 (MQ=255)                                                                                                                                                                                                 gCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAGGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCtt                                                          <  2:426568/250‑1 (MQ=255)                                                                                                                                                                                                     cgcgGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGACCGTCGTACCAAACAGCAG‑GCCCAGCCCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCCCCCGGCTTCATGATGTCGACACCTGTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATa                                                     <  2:317198/251‑1 (MQ=255)                                                                                                                                                                                                       cgGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGGCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAAGAGCAACAGCACGGCTTTGATAAt                                                   <  1:137871/251‑1 (MQ=255)                                                                                                                                                                                                       cgGGCATC‑GATGTCTGTAACGTAGCGCATCAGAATGTGCGGCAAGACCGTCGTACCAAAGATCAG‑TCACAGACCCACAGAAAGCGCGGAG‑ATCGGCTCATACACCAGCACGACAGCCTTCCTGATGTCGACACCTTTCGGGTGTACCCCCACCGCTTCACTGACCAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCAGGCACCGAACCGCAACAGCACGGATTTGAtcat                                                   >  2:585128/1‑248 (MQ=255)                                                                                                                                                                                                         ggCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCAACATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAAttt                                                 >  1:45482/1‑251 (MQ=255)                                                                                                                                                                                                           cATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCCG‑TCCCAGACCCCGAGAGAGCGCGGAG‑ATCGGCTCTTTCACCAGCCCGCCAGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGCTTGTTGAAGCTAAAGCCGACGTGTTTCATCAACATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGAACGGCTTTGATAATTTGc                                               >  1:33106/1‑251 (MQ=255)                                                                                                                                                                                                                  tGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTAGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCACGCCCGGCTTCATGATGTCAACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCAccc                                           >  2:227313/1‑249 (MQ=255)                                                                                                                                                                                                                       ggaaaGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAggtggg                                    <  2:429249/249‑2 (MQ=255)                                                                                                                                                                                                                       gTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACACCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGAGGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCGCGTCGTTGATAAGTTGCACGCAggtggt                                    <  2:342182/251‑1 (MQ=255)                                                                                                                                                                                                                                    tCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATgccg                       >  1:162515/1‑251 (MQ=255)                                                                                                                                                                                                                                       gAATGTGCGGCAAGCCCGTCGTACCAAACATCAG‑TACCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGATTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCAACATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCCGGTAGTCGCCAGCCTGccgccg                    >  1:411846/1‑251 (MQ=255)                                                                                                                                                                                                                                           gtgCGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACa                <  1:54505/251‑1 (MQ=255)                                                                                                                                                                                                                                              cGGCAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGa             <  1:270113/251‑1 (MQ=255)                                                                                                                                                                                                                                                 cAAGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAACGGCCATAAAGCTGGCCCCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGt          >  2:400173/1‑251 (MQ=255)                                                                                                                                                                                                                                                   aGCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGCG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTAc        >  1:532441/1‑251 (MQ=255)                                                                                                                                                                                                                                                    gCCCGTCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTAca       <  1:312559/251‑1 (MQ=255)                                                                                                                                                                                                                                                         ccgTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACTCCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAAGTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTAcatcatc  <  2:114304/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                             CGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGT‑GAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATC‑GCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAG‑TCCCAGACCCAGAGAGAGCGCGGAG‑ATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTACATCATC  >  NC_000913/4283831‑4284327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑