Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,278,488	C→T	P4S (CCA→TCA)	ghxP →	guanine/hypoxanthine transporter GhxP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,278,488	0	C	T	100.0%	163.4 / NA	48	P4S (CCA→TCA)	ghxP	guanine/hypoxanthine transporter GhxP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/23);  total (25/23)

ATCGTCAAGACAATAAAAGAGAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCTGGTTGCCGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATT  >  NC_000913/4278242‑4278733                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        aacGTCAAGACAATAAAAGAGAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTAGGCGCGGAGCTAAACGGTTGCTTTTTTGCGACGCATCAAATTGTCTCAAACCTGGAGCAGGAATATAACGTTGCGCTGGCAGGGGATTGTCCGCCACGCTTCTTTACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATc                                                                                                                                                                                                                                                     <  1:533037/249‑1 (MQ=255)              tAAAAGAGAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACcggc                                                                                                                                                                                                                                        >  2:12313/1‑251 (MQ=255)               aaaaGAGCAAAAAGCAGCAACCTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCAACGCAGCAAATTGTCGCAAACCTGGAGCAGGCAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGAATCTTGACGAAAATTAAACACTCAGGGGATGTTTTCTTATGTCTACGTCCTCAGCCAGTACcggcg                                                                                                                                                                                                                                       >  2:357548/1‑251 (MQ=255)                  agagAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTGTTTGCGACGCAGCAAATTGTTGCAAACCTGGAGCAGGAAGATAACGTTGCGCTGGCAGGGGATTTTCCGCCACGCAGCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCATCGCGTACCGGCGGtt                                                                                                                                                                                                                                    <  2:52024/251‑1 (MQ=255)                   gagaAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCTTTTGCTTTTTTGACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTc                                                                                                                                                                                                                                   <  1:448526/251‑1 (MQ=255)                          aaGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCTCCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGTATGTGTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGAc                                                                                                                                                                                                                            <  2:201373/251‑1 (MQ=255)                                               aaaGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCCACGCCTGGTTT‑ATAAATTcacc                                                                                                                                                                                                       >  2:313453/1‑249 (MQ=255)                                                aaGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAAc                                                                                                                                                                                                     <  2:305028/251‑1 (MQ=255)                                                      cTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTACGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGGTCACCCGACCACCGGTTT‑AAAAATTCCCAAcacggg                                                                                                                                                                                                >  1:324307/1‑245 (MQ=255)                                                                cGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAACTTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTTACTCCACGCCTGGTTT‑AAAATTTCACAACATgggatacccgttcc                                                                                                                                                                                     >  2:513117/1‑239 (MQ=255)                                                                cGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCCGGGTT‑AAAATTTTACAAAGTGGGAGCACTGTCCg                                                                                                                                                                                     >  1:13845/1‑251 (MQ=255)                                                                 ggATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATc                                                                                                                                                                                                                                                     <  1:213826/187‑1 (MQ=255)                                                                 ggATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATc                                                                                                                                                                                                                                                     >  2:213826/1‑187 (MQ=255)                                                                  gATAATCGTTTGCTTTTTTTACCACACGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTTACTCGACGCCTGGTTT‑TAAAttttccaacgagggaaacctgtccctc                                                                                                                                                                                   >  2:298540/1‑227 (MQ=255)                                                                        cGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTAACTCTACGCCTGGTTTTAAAATTT‑ACCACGTGGAGTCACTGTTCGTCACGaa                                                                                                                                                                              >  2:177268/1‑250 (MQ=255)                                                                                        caccCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGG‑TTaac                                                                                                                                                            <  2:536757/251‑1 (MQ=255)                                                                                          cccGTTTTGTATGAGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTACACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTTAATCGACGACTGGGAT‑GAAAATTAAAAAAGGGGGAGCACTGGCCGTAAGGGAGTGGTTGCCGGG‑GTaacc                                                                                                                                                           >  1:94996/1‑249 (MQ=255)                                                                                                      gcgcGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGG‑TTAACAACGTTTCTGGCg                                                                                                                                               <  2:45957/250‑1 (MQ=255)                                                                                                             gCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAACGTGGAAGCACTGTCCGTCAGGGAGTGGTTGCCGGG‑TTAACAACGATTCTGGCGATGGTCt                                                                                                                                        >  1:458916/1‑250 (MQ=255)                                                                                                                       tGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCGTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGG‑TTAACAACGttt                                                                                                                                                     <  1:385176/227‑1 (MQ=255)                                                                                                                           tttttGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCAtcgtc                                                                                                                          <  1:162750/250‑1 (MQ=255)                                                                                                                               tGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTGCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGACTACGTCATCAGCGCGTACCGGCGGTTCACGCGACGCCTGGTTT‑AAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGTG‑TTAACAACGGTTCTGGCGATGGTCTACTCGGTCATCGTCGTTc                                                                                                                      <  1:485951/250‑1 (MQ=255)                                                                                                                                  gACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGATCACTCCACGCCTGGTTT‑TAAATTTTACAAAGTGGGAGCACTGTCCGTCAGGAAGGGGTTGCCGGG‑GTAACAACGTTTCTGGCGATGGTCTACTCGGtacacgtcgtttccgg                                                                                                                  >  2:392487/1‑236 (MQ=255)                                                                                                                                     gcagcaAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑TAAAATTCCCAACGGGGGAGCACTGTCCGTCAGGGAGTGGGTGCCGGG‑TTAACAACGTTTCTGGCGGTGGTCTTCTCGGTCATCGTCGTTcccgggat                                                                                                               >  1:483537/1‑245 (MQ=255)                                                                                                                                      cagcaAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATg                                                                                                              <  1:70565/251‑1 (MQ=255)                                                                                                                                                 cGCAAACATGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGCTTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTTACTCGACGCCTGGTTT‑AAAATTTTCCAAAGTGGGAGCACTGTCCGTCAGGAAGGGGTTTCCGGGGTTAA‑AACGTTTTTGGCGGTGGGCTACTCGGGCACCGTCGGTCCCGGGATGTTGGGGAAAGc                                                                                                   >  1:159658/1‑251 (MQ=255)                                                                                                                                                   cAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGGCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGG‑TTAACAACGTGTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAg                                                                                                 <  2:199003/251‑1 (MQ=255)                                                                                                                                                            aGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTTACTCCGCGCCCGGGTT‑AAAATTTTACAAAGTGGGAGCACTGTCCGTCCGGAGGGGGGTGCCGGG‑GTAACAACGTTTCTGGCGGTGGGCTACTCGGTCATCGTCGTTCCCGGGATGTTTGGTAAAGCAGGCTTcccc                                                                                         >  2:418074/1‑249 (MQ=255)                                                                                                                                                            aGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTccc                                                                                          <  1:418074/249‑1 (MQ=255)                                                                                                                                                              cAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCt                                                                                      <  2:133441/251‑1 (MQ=255)                                                                                                                                                                   aGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGGTTACTCGACGCCCGGTTT‑TAAATTTCCCCACCTGGAAGCACATTCCCTCAGGAAGGGGTTGCCGGGGTTAAAAAGGTTT‑TGGCGATGGTCTACTCGGTCATCGTCGTTCCCGGGCTGTTTGGTAAAGGCGGCTTCCCCCCtcgcgc                                                                                 >  2:246960/1‑246 (MQ=255)                                                                                                                                                                     aTAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGCCGCCTGGTTT‑AAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGG‑TTAACAAAGTTTCTGGCGGTGGGCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCCCCTGCGGc                                                                                 >  1:68546/1‑249 (MQ=255)                                                                                                                                                                                   gCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTTACAACGTGGGAGCACTGTCCGGCAGGAAGGGGTTGCCGGG‑TTAACAACGTTTTTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGGATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCCGTTTTCGTTGCaaac                                                                 >  1:208441/1‑249 (MQ=255)                                                                                                                                                                                       gggATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTTACTCGACGCCTGGTTT‑AAAATTTCACAACGTGGGAGCACTGTCCGGCAGGAAGGGGTTGCCGGG‑GTTACAACGTTTCTGGCGATGGGCTACTCGGTCATCGCCGTTCCAGGTATGTTGTGTAAAGCAGGCCTCCCCCCTGCGGCAGTTTTTGTTTGAACCTGtt                                                             >  2:156573/1‑250 (MQ=255)                                                                                                                                                                                             gTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCTGGTTg                                                       <  1:313453/251‑1 (MQ=255)                                                                                                                                                                                                  ccACGCATCTTGACGAACATTACACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAAATTCACCACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGG‑TTAACAAAGTTTCTGGCGATGGTCTACTCGGGCATCGTCGTTTCAGGTATGTTGGGGAAAGCAGGCTTCCCGCCTGCGGCAGTTTTTGTTGGAAACTGTCTGGTTGCCggg                                                  >  1:173065/1‑250 (MQ=255)                                                                                                                                                                                                   cccGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGGCTCCGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAACTTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCTGGTTGCCGGAc                                                 <  2:483537/249‑1 (MQ=255)                                                                                                                                                                                                     cGCACCTTGACGAAAATTAAACTCGACGGTGATGTTTCTTTAGGTCTACTTCAGTAGCGGGTACCGGCGGTTCACTCGACGCTTGGTTT‑AAATTTTCACAACGTGTAAGCACTGTCCGTCATGAAGTGGTTGCCGGT‑TTAACAACGTTTCTGGCGATGGTCGACTCGGTCATCGGCGTCCCAGTTAGGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACTTGGCTGGTTGCCGGACTc                                               <  2:395297/251‑1 (MQ=255)                                                                                                                                                                                                         tCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCCTCAGCGCGTACCGGCGGTTTACTCGCCACCTGGATT‑TAAATTTCACAACGGCGAAGCACTGTCCCTCAAGAAGTGGTTGCCGGG‑TTAAAAAACTTTCTGGCGATGGTCTACTCCGGCAACGTCGTTCCCGGGATGTTGGGTAAAACAGGCTTTCCGCCTGCGGCAGGTTTCCTTGCAAACTGTCTGGGTGCCggccccgggt                                           >  1:267621/1‑243 (MQ=255)                                                                                                                                                                                                              aCGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGAAGACTGGTTT‑AAAATTTCACCACGGGGGAGCACTTTCCGTCAGGTAGTGGGTGCCCTG‑TTAAAAACGTTTCTGGCGGTGGTCTACTCGGTCATCGTCCTTCCCGGGCTTTTGGGTAAAGCAGGCTTCCCGCCCGCGGAGGGTTTCGTTGCAACCTGTCTGGGTGcccggcccgggtctttt                                      >  1:479914/1‑236 (MQ=255)                                                                                                                                                                                                                gAAAATTAAACTCTCAGGGGATTTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTGGCCGGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCTGGTTGCCGGACTCGGTTCTATCGt                                    <  1:58942/251‑1 (MQ=255)                                                                                                                                                                                                                 aaaaTTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGGCGCCTGGTTT‑AAAATTTCACAACGTGGGAGCACTGTCCGTCCGGAAGTGGTTGCCGGG‑GTAAAAACGTTTCTGGCGATGGGCTACTCGGTCCTCGGCGTTCCAGGGATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGGATGGTTGCCgggatcgggtctttcggg                                   >  1:187262/1‑235 (MQ=255)                                                                                                                                                                                                                 aaaaTTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCTGGTTGCCGGACTCGGTTCTATCGTg                                   <  1:246960/251‑1 (MQ=255)                                                                                                                                                                                                                  aaaTTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCTGGTTGCCGGACTCGGTTCTATCGTGa                                  <  1:120521/251‑1 (MQ=255)                                                                                                                                                                                                                    aTTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTTCCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAACGTGGAAGCACTGGCCGTCAGGAAGTGGTTGCCGGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCTGGTTGCCGGACGCGGTTCTATCGTGATg                                <  1:227577/251‑1 (MQ=255)                                                                                                                                                                                                                         aCTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTTACTCTGCGCCCGGTTT‑TAAATTTTACAACGTGGAAGCACTGTCCGTCAGGAAGGGGTTGACgg                                                                                                                                                                   >  2:420793/1‑116 (MQ=255)                                                                                                                                                                                                                         aCTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGTTTGCCgg                                                                                                                                                                   <  1:420793/116‑1 (MQ=255)                                                                                                                                                                                                                                                  cTACGTCATCAGCGCGTACCGGCGGTTTCCCCGCCGCCCGGTTT‑AAAATTTCACCACGTGGGAGCACCGTCCGTCAGGGAGTGGTTGCCGGG‑TTAACAACGTTTCTGGCGCTGGTCTACTCGGGCATCGGCGTTCCCGGTATGTTGGGTAAAGGAGGCTTCCCCCCTGCGGCAGGTTTCGTTGCAAACTGTCTTGGTGCCGGGCTCGGTTCTCTCTTGATGGGTCTGTGGGCTAATCTGCCgcttgggatt  >  2:463447/1‑242 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        ATCGTCAAGACAATAAAAGAGAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCTGGTTGCCGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATT  >  NC_000913/4278242‑4278733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑