| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,249,684 | C→T | A44V (GCT→GTT) | malM → | maltose regulon periplasmic protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,249,684 | 0 | C | T | 98.6% | 244.4 / NA | 71 | A44V (GCT→GTT) | malM | maltose regulon periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (47/23); minor base A (1/0); total (48/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATCCGGCATAAAAACAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGCTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGACCCCATCAGCCTTCTTCCCC > NC_000913/4249438‑4249928 | cATCCGGCATAAAAACAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTgttgcg > 2:9352/1‑251 (MQ=255) ccGGCATAAAAACAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTgttgcgctg < 1:271339/251‑1 (MQ=255) ccGGCATAAAAACAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTgttgcgctg < 1:181628/251‑1 (MQ=255) aaaCAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTc > 2:270284/1‑251 (MQ=255) aaCAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTc > 2:41016/1‑250 (MQ=255) tGTCATTATCTGCAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCACCAACTCACCTGGac > 1:484600/1‑251 (MQ=255) cATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGCATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACAcc > 1:371523/1‑251 (MQ=255) cATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGacac < 2:479129/250‑1 (MQ=255) aTCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTc > 1:351974/1‑251 (MQ=255) tCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCACCAACTCACCTGGACACCGGTCg > 2:462298/1‑251 (MQ=255) gAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCAGTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTc < 2:60448/247‑1 (MQ=255) aGGGGCGACAGCCCCTCTGATTATCGGGTTTAGCGCGCTCTTGCCTGGCTACCGCTGATCTCAAGATTTTGAGGTGACAACAATGAAAATGACTAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAGGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCAGATGCGCTGCAACAACTCACCTGGACACCGATCGAACAAt > 2:544394/1‑251 (MQ=255) cGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTaa > 2:359798/1‑250 (MQ=255) gATTATAGGGTTGAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTGGATGTGAAAACAATGAAAATGAAAAAAAGTCTCATCGTCCTCTTTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATGAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCACCTACTCGCCTGGACACCTGTCGATCAATCTAAACCCCAGaccacc < 2:300936/250‑1 (MQ=255) gcTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGcc > 2:117550/1‑251 (MQ=255) gCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGGTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCcaacaa < 2:263786/250‑1 (MQ=255) ggCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAAGAAAAGGCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCCATCGCGCCTGGAATTATCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCTGACAACAACTGaa < 2:25927/251‑1 (MQ=255) ccGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTc > 2:496357/1‑251 (MQ=255) cGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAATAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACGCCCCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACGGAACGTTcc < 1:419066/251‑1 (MQ=255) gCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTccc < 2:495136/251‑1 (MQ=255) gCTGAGATCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTccc > 1:469674/1‑251 (MQ=255) cAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGt > 1:482768/1‑251 (MQ=255) gTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTtgc > 1:177013/1‑251 (MQ=255) tGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTtgct > 1:245396/1‑251 (MQ=255) gAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTtgctg > 1:446736/1‑251 (MQ=255) aaCAATGAAAATGAATAAAAGTCTCATCGTCGTCTGTTTATCAGCAGCGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGCTGTTAACTACGTAACGCAAAACACCAGCGAAGCGACAGCCATTCCATCTGTTGCGATGCAACAACTCACCTGGACACCGGTCCATCAATCTAAAACCCAGACCACCCAACAGGAGTCCGGCGACCAACAAATGAACGTTCCCGGCATCAGAGGTCCGGTTGCTGCGt > 1:244412/1‑251 (MQ=255) aaaatgaaTAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGt > 1:190561/1‑251 (MQ=255) aaaatgaaTAAAAGTCTCATAGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGt > 1:224264/1‑251 (MQ=255) tgaaTAAAAGTCTCATCGACCTCTGTTTATCAGCAGGGTTACTGACAAGCCCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAAAACCCGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGCCACCGGTCGATCAATCTAAAACCCAGACCACACAACTGGCGACCGGCGGCCAACAACTGAACGTCCCCGCCATCAGTGGTCAGGTTGCTGCGTACAGCGTCCCg > 1:72313/1‑251 (MQ=255) aaTAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGc > 1:114563/1‑251 (MQ=255) aTAAAAGTCACATCGTCCTCTGTTTAGCAGCAGGGTTACTGGCAAGCTCGCCTTGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCAGTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTTGAGCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGGACGTTCCCGGCATGAGTGGTCCGGGTGCTGCGTACAGCGTCCCgtca < 2:484600/251‑4 (MQ=255) aaGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAAc > 1:266341/1‑250 (MQ=255) aGTCTCATCCTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTCCGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACACAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCCAACAt > 2:377923/1‑251 (MQ=255) gTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCa < 1:434755/151‑1 (MQ=255) gTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCa > 2:434755/1‑151 (MQ=255) aTCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGcc < 2:314907/184‑1 (MQ=255) aTCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGcc > 1:314907/1‑184 (MQ=255) tcgtcaactgtttcaccgcagGGTTACTGGCACGCGCACCTGGAATTACACTTGCCGCTGCTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGCTCAATCTAAAACACCGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGAAATCAGTGGTCCGGTGGCTGCGTACAGCGTCCCGACAAACATTGGCGa > 2:551576/18‑250 (MQ=255) tATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACg > 1:145743/1‑251 (MQ=255) cagcagGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTg < 1:558889/251‑1 (MQ=255) agGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGAcc > 1:206292/1‑250 (MQ=255) aCTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAg < 1:543401/251‑1 (MQ=255) aCTGGCAAGCGCGCCTGGAATTAGCCTTGACGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGGCCCGGCAAACATGGGCGAACTGACCCTGAAGCTGCCCAGCGaa > 2:204665/1‑250 (MQ=255) ggCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTg < 1:270284/250‑1 (MQ=255) cAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACAAGCGAAGTGaac > 1:458936/1‑251 (MQ=255) gcCTGGAATTAGCCTTGACGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCAAGCCCTTCAATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATAAGTGGTCCGGTTGCTGAGTCAAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAacaaacaa > 2:572532/1‑250 (MQ=255) ccTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACaaacaaac < 2:190561/251‑1 (MQ=255) tGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAAcc > 1:437286/1‑250 (MQ=255) aaTTAGCCTTGCCGATGTTAACTACGTACCGCAAAACCCCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTGCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCg < 2:351974/251‑1 (MQ=255) aGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGtttt < 2:216633/251‑1 (MQ=255) cTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTgc > 1:231621/1‑251 (MQ=255) cTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGGTGCTGCGTACAGCGTCCCGGCACACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCACCGTTTTTgc > 2:21346/1‑251 (MQ=255) gATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGa > 1:292859/1‑250 (MQ=255) gTTAACTACGTACCGCAAAACGCCAGCGACGCTCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTTCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGt < 1:287151/251‑1 (MQ=255) gTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGt > 2:566407/1‑218 (MQ=255) gTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGt < 1:566407/218‑1 (MQ=255) tAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCACCAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGc > 1:37545/1‑251 (MQ=255) aaCTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGCCCCTGACGCTGACCAGCGAAGTGAACAAACACACCAGCGTTTTTGCGCCGAACGTGCt > 1:364781/1‑251 (MQ=255) aaCTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCt > 2:433430/1‑251 (MQ=255) cTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTg > 1:104504/1‑250 (MQ=255) aCGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGAt < 2:159861/250‑1 (MQ=255) tACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCtt > 1:258177/1‑251 (MQ=255) ccGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGCAGTGCACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTg > 2:372833/1‑250 (MQ=255) cGCAAAACACAAGCGACGCGCCAGCCATTACATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACAACACAACGGGCGACCGGCGGCCAACAACTGACCGTTCCCGGCATCAGTGGTCCTGTTGCTGCGTACAGAGTCCGGGCAAACATGGGAGAACTGACCCTGACGATGACCAGCGAAGTGAACAAAAAAACCAGCGTTTTTGCACCGGACGAGCAGAGTCTTGAt > 1:480597/1‑251 (MQ=255) gCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGGACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATc > 2:138050/1‑251 (MQ=255) cAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCACCAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATc > 2:147766/1‑250 (MQ=255) aaaaCACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTTCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTGTTGCGCCGAACGTGCTGATTCTTGATCAg < 1:430126/251‑1 (MQ=255) aaaCACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGTTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAATTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGa < 1:361028/251‑1 (MQ=255) aCGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGAcccc > 1:547503/1‑250 (MQ=255) cgcCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGACCCCATCa < 1:496357/251‑1 (MQ=255) gccagccaTTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTGTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGACCCCATCAg < 2:99112/251‑1 (MQ=255) ttCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACAGGCGGCCAACAACTGAACGTTACCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGCACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGCTTCTTGATCAGAACATGACCCCATCAGCCttcttc > 2:512125/1‑251 (MQ=255) ccATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCAAACGTGCTGATTCTTGATCAGAACATGACCCCATCAGCCTTCTTccc > 2:445801/1‑251 (MQ=255) aTCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTACAACCCAGACCACCCAACTGGCGACCGGCGGCCCACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGCCCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGACCCCATCAGCCTTCTTcccc > 1:578488/1‑250 (MQ=255) | CATCCGGCATAAAAACAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGCTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGACCCCATCAGCCTTCTTCCCC > NC_000913/4249438‑4249928 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |