Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,234,479	C→T	A241V (GCG→GTG)	pgi →	glucose‑6‑phosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,234,479	0	C	T	100.0%	182.4 / NA	59	A241V (GCG→GTG)	pgi	glucose‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/29);  total (30/29)

GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTG  >  NC_000913/4234238‑4234718                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                ggCCCATACATGGTGAACGAAGCTCTGCGTCCGTACAAAACCCACCTGAACATGCAATTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTc                                                                                                                                                                                                                                        >  2:369041/1‑251 (MQ=255)  gCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTcc                                                                                                                                                                                                                                       <  1:276217/251‑1 (MQ=255)        aCATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAAt                                                                                                                                                                                                                                 >  1:535076/1‑251 (MQ=255)            ggTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTTAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGTTGAAAAACACGTTGCATACCACTTTGTGGTGCTTTCCACCAATGcc                                                                                                                                                                                                                              >  1:432562/1‑250 (MQ=255)                 ccGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGcc                                                                                                                                                                                                                        <  1:490022/251‑1 (MQ=255)                             gTCCGTACAAAAACCACCTGCACATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGGGATGAAAAACACGTTGCAACACACTTTGTGGCGCTTTCCCCCAATGCCACAGCCGTTGGCGAGttt                                                                                                                                                                                                            >  2:558897/1‑251 (MQ=255)                                      aaaaCCACCTGACCATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGCGTTTGGTATTGAt                                                                                                                                                                                                   >  2:290973/1‑251 (MQ=255)                                        aaCCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATAc                                                                                                                                                                                                 >  1:4144/1‑251 (MQ=255)                                         accgccTGAACATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACGCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACGTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACt                                                                                                                                                                                                <  2:287042/247‑1 (MQ=255)                                             cctgaaaatgccctgagtgtCTAACGTCGATGGGCCGCACAGCGCGGAAGTGCTGAAAAAATTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAATCCTTCACCACTCAGTAAACGAGGACCAAATCCCATAGCGCGCGTGACTGGTGCCTGAAAGCGGCAGGGGATGAAAAACACGTTGCAAAACACTTTGTGTCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCa                                                                                                                                                                                            <  2:317321/232‑1 (MQ=255)                                                gAACATGCGCTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCaa                                                                                                                                                                                           <  2:456204/249‑1 (MQ=255)                                                      gCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCg                                                                                                                                                                                   >  1:229405/1‑251 (MQ=255)                                                      gCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCg                                                                                                                                                                                   >  1:477285/1‑251 (MQ=255)                                                      gCACGTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTc                                                                                                                                                                                    <  1:558897/250‑1 (MQ=255)                                                        aCTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGGCCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAg                                                                                                                                                                                 <  1:274982/251‑1 (MQ=255)                                                               ttCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTgg                                                                                                                                                                           <  1:410759/250‑1 (MQ=255)                                                                      gTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCAGCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGt                                                                                                                                                                   <  1:301105/251‑1 (MQ=255)                                                                         gATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGCTGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGAATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTgg                                                                                                                                                                >  1:551787/1‑251 (MQ=255)                                                                            gggACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGCCTGGGTTggcgg                                                                                                                                                             >  2:512432/1‑251 (MQ=255)                                                                                       cgcgGAAGTGCTGAAACAAGAAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTAACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCACACCTGTTCGAGTTCTGGGACTGGGTTGGCGGCCGATACTCtt                                                                                                                                                  >  2:464240/1‑251 (MQ=255)                                                                                           gAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACGCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTtgtg                                                                                                                                              <  1:565263/251‑1 (MQ=255)                                                                                             aGTGCTGAAAAAAGAAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAACCACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGt                                                                                                                                            >  2:249619/1‑251 (MQ=255)                                                                                                gCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACGCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCTCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAGTGATACTGCCAACAGGTTCGAGTTCTGGGACTGGGGTGGCGGCCGTTACTCTTTGTGGTCAg                                                                                                                                         <  2:181015/251‑1 (MQ=255)                                                                                                     aaaaaGTAAACCCGTAAACCACGCTGTTCTTGGTAGCATCTAAAACCTGCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGTCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGGTCGAGTTCTGGGACTGGGTTGGCGGCCTTTACTCGTTGTGGTCTGCGAtt                                                                                                                                    <  2:380183/251‑1 (MQ=255)                                                                                                                 cGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGAtt                                                                                                                        >  1:127301/1‑251 (MQ=255)                                                                                                                    aaaCCACGCTGTTCTTGGTAGCTTCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGAGAGCGTGACTGGTTACTGAAAGCGGCAGGAGAAGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATCATGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGtt                                                                                                                     >  2:112476/1‑251 (MQ=255)                                                                                                                      accccgcggTTCTTGTTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACATTTTGTGGCGCTTTCCTCCAATGCCAAAGCCGTTGGCGAGTTCGGTATTGCTACTGCCAACAGGTTCGAGTGCTGGTACTGGGTTGGCGGCTTTTTCTCTCTTTGGTCAGCGATTGGCCTGTCGCTTTTtct                                                                                                                   <  2:307987/243‑1 (MQ=255)                                                                                                                             tGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATc                                                                                                            >  2:99190/1‑251 (MQ=255)                                                                                                                              gTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAATCTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCCTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGAGACTGCCAACATGTTCGAGTTCTGGGACTGGCTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATc                                                                                                            <  1:257107/250‑1 (MQ=255)                                                                                                                                tCTTGGTAGCATCTAAAACCGTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCTTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCgg                                                                                                          <  1:498639/250‑1 (MQ=255)                                                                                                                                    ggTAGCATCTAAAACCTTCACCACTCAGTAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGTCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTg                                                                                                     <  2:340347/251‑1 (MQ=255)                                                                                                                                       aGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATa                                                                                                  <  2:535076/251‑1 (MQ=255)                                                                                                                                         cATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAAc                                                                                                <  1:410315/251‑1 (MQ=255)                                                                                                                                             tAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGAGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCg                                                                                            >  1:584963/1‑251 (MQ=255)                                                                                                                                              aaaaCCTTCACCTCTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAATCACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTc                                                                                             <  1:535401/249‑1 (MQ=255)                                                                                                                                                             cAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGGTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTcc                                                                              <  1:527359/249‑1 (MQ=255)                                                                                                                                                               ggAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGTCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAAc                                                                                      <  2:394102/239‑1 (MQ=255)                                                                                                                                                               ggAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAAc                                                                                      >  1:394102/1‑239 (MQ=255)                                                                                                                                                                         cccatcGCCCATAGCGTGCGGGACTTGTTCCTGAAATCGGCAGGTGATGAAAAACACGTGGCAAAACACTTTGTGGCGCTTTCCTCCAATGCCAAAGCCGTGGGCGAGTTTGGTATTTATACTGCCAACATGTTCGAGTGCTGGGACTGGGTTGGCGGCCGTTGCTCTTTGTGGTTAGCTATTGGCCTGGCGATTGTTCCCTCCAGCGGCTTTGATAACTTCGTTGAACTGCTTTCCCGCGCACTCGCGAt                                                                <  2:233980/246‑1 (MQ=255)                                                                                                                                                                           cAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTTGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATgg                                                              <  1:259818/251‑1 (MQ=255)                                                                                                                                                                            aaCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGGTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCGCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGa                                                             <  1:369041/251‑1 (MQ=255)                                                                                                                                                                            aaCGCACATAGCGCGAGTGACTGGTTCATGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATgg                                                              >  2:337658/1‑250 (MQ=255)                                                                                                                                                                                            gTGACTGGTTACTGAAATCGGCAGGTGACGAAAAACACGTTGCAAAACACTTTCTGGCGCTTTCCACCCATGCCAACGACGATGGCGCGTTTGGTATTGATACTGCCAACATGTTCGAGTTATGGGACTGGGTTGGCGGCCGTTACTCTTTGTAGTCAGCGATTGGCCTGTCGATTGTTTTCTCCATCGGCTTTGATAACTTCGTTGAACTGGTTTCCGGCGCACACGCGATGGACAAGCATTTCTccacc                                             >  2:270156/1‑251 (MQ=255)                                                                                                                                                                                              gACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTGCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTccacca                                            <  1:399664/250‑1 (MQ=255)                                                                                                                                                                                                  ggTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCt                                       >  2:156828/1‑251 (MQ=255)                                                                                                                                                                                                    ttCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGCTTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGc                                     >  2:404360/1‑251 (MQ=255)                                                                                                                                                                                                       cTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCgg                                  >  2:174566/1‑250 (MQ=255)                                                                                                                                                                                                               ggCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAAcc                          >  1:450637/1‑251 (MQ=255)                                                                                                                                                                                                                    gTGATGAAAAACCCGTGGCAAACCACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCTTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAAccggcct                     <  2:476421/251‑1 (MQ=255)                                                                                                                                                                                                                         gaAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGgcgc                                                                         >  1:70803/1‑194 (MQ=255)                                                                                                                                                                                                                         gaAAAACACGTTGCAAAACACGTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGgcgc                                                                         <  2:70803/194‑1 (MQ=255)                                                                                                                                                                                                                           aaaaCTCGTTGCTAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAATCCTGCCTGTActgc              <  2:433281/251‑1 (MQ=255)                                                                                                                                                                                                                             aaCACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTActgctg            >  1:243103/1‑251 (MQ=255)                                                                                                                                                                                                                               cacGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGgc          >  2:245220/1‑251 (MQ=255)                                                                                                                                                                                                                                acGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGgcg         >  1:230536/1‑251 (MQ=255)                                                                                                                                                                                                                                    tGCAAACCACTTTGTGGCGCTTTCCAACAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTATGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTg      >  2:534056/1‑250 (MQ=255)                                                                                                                                                                                                                                     gCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACGGGGTTGGCGTCCGTTACTCTTTGTGGTCAGCGATTGGCCTGGCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTGCTCCACCACGCCTGCCGAGAAAAACCTGCCGGTACTGCTGGCGCTGAt    <  2:204786/251‑1 (MQ=255)                                                                                                                                                                                                                                      cAAAACACTTTGTGGAGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGCCTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGAtt   >  1:497761/1‑251 (MQ=255)                                                                                                                                                                                                                                       aaaaCACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTg  >  1:438542/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTG  >  NC_000913/4234238‑4234718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑