Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,222,743 C→T intergenic (‑256/+61) arpA ← / ← iclR regulator of acetyl CoA synthetase/DNA‑binding transcriptional repressor IclR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,222,7430CT98.2% 180.7 / ‑5.7 55intergenic (‑256/+61)arpA/iclRregulator of acetyl CoA synthetase/DNA‑binding transcriptional repressor IclR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (37/17);  minor base A (0/1);  total (37/18)
Fisher's exact test for biased strand distribution p-value = 3.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

TTATTGATGTCTTGAATTTTAACTATCAGAACAGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACCCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCC  >  NC_000913/4222504‑4222987
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    
ttATTGATGTCTTGAATTTTAACTATCAGAACAGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTAAATCTCATAATGCAGCCGTAAAATTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTAtt                                                                                                                                                                                                                                           >  1:3243/1‑251 (MQ=255)
  aTTGATGTCTTGAATTTTAACTATCAGAACAGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAAGCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCTATTGTGTTAATAATTAAGTGGTTTTATGTTTAAAAATAATGCTGATTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACACTTATTCTATTg                                                                                                                                                                                                                                          <  1:238253/250‑1 (MQ=255)
            tGAATTTTAACTATCAGAACAGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAgg                                                                                                                                                                                                                                 <  2:478129/249‑1 (MQ=255)
                   tAACTATCAGAACAGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATggg                                                                                                                                                                                                                        >  1:450678/1‑251 (MQ=255)
                             aaCAGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGTTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATGTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACTCTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTg                                                                                                                                                                                                              <  2:48047/251‑1 (MQ=255)
                                    aaaaaaTTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGc                                                                                                                                                                                                       >  2:51219/1‑251 (MQ=255)
                                       aaaTTTAATATGATTACAACTAAAGAAATATCATAAAACGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGAGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAATGTGTGTGAAGTGTATGACATTAAGTTATTATGGCGTCAATGAGATTAACAGACACTATTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCg                                                                                                                                                                                                    >  2:119372/1‑251 (MQ=255)
                                               tatGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGaaa                                                                                                                                                                                             >  2:281631/1‑250 (MQ=255)
                                                atGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTGCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGaaaaa                                                                                                                                                                                           <  1:51219/251‑1 (MQ=255)
                                                      aCAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTATTAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAgc                                                                                                                                                                                     <  2:184306/251‑1 (MQ=255)
                                                       cAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAgcg                                                                                                                                                                                    >  1:369600/1‑251 (MQ=255)
                                                           tAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCAtt                                                                                                                                                                                >  1:111793/1‑251 (MQ=255)
                                                              aGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTcc                                                                                                                                                                              >  1:248980/1‑250 (MQ=255)
                                                              aGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGCCAATGCGATTAACAGACCCTCTTATTCTATTGCCACTAAGGTATGATGGGCAGAATATGGCCTCTGCCCGCCAGAAAAAGTCAGCGCATGCCa                                                                                                                                                                             <  2:369600/251‑1 (MQ=255)
                                                                aaaTATCATAACTCGCTCAATCTCATAATGCAGCCGTAAAAGATTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCTTCTGCACGCCAGACAAAGTCAGCGCATTCCAcc                                                                                                                                                                           >  2:378409/1‑251 (MQ=255)
                                                                   aatCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTa                                                                                                                                                                        <  2:450678/250‑1 (MQ=255)
                                                                                          aaTGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTcgcc                                                                                                                                                  <  1:299135/250‑1 (MQ=255)
                                                                                                   ggaaaaGTTTCGGGGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATc                                                                                                                                         >  2:115300/3‑250 (MQ=255)
                                                                                                   gTAAAAGCTTCGGAGGAATGAGATCTTGCGATTTTCTTACTAATTAAGTGGTTTGATCTTTAAAACTAATGATGAGTTAATAAAAGTGTGTGAAGTGTCTGCCATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTATATTGCCACTCAGGTATGATGTGCAGAATATTGCCTCTGCCAGCCAGAAAAAGTCAGCGCATTCCACCGTAAGCCAGCGTCACTTCCTTCGCCGCTTTAATc                                                                                                                                         >  2:243047/1‑250 (MQ=255)
                                                                                                         gTTTCGGTGGAATGAGATCTTTCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGTGTCAATGCGATTAACAGACACTCTTATGCTATTGCCACTCAGGTATGATGGGTAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATcg                                                                                                                                  <  1:401080/251‑1 (MQ=255)
                                                                                                             cGGTGGAATGAGCTCTTGCGATTGTGTTACTACTTAAGTTGTTTGATGTTTACCAATAATGCTGAGTTAATACAAGTGAGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATTATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCATCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGcc                                                                                                                              >  2:466090/1‑251 (MQ=255)
                                                                                                                             tGCGATTTTGGTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGGGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCAGCGCGCCAAACTCGGTCAc                                                                                                                  <  1:115300/247‑1 (MQ=255)
                                                                                                                              gCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGt                                                                                                             <  1:281631/251‑1 (MQ=255)
                                                                                                                               cGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATcg                                                                                                                                  >  1:214592/1‑229 (MQ=255)
                                                                                                                               cGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGGCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATcg                                                                                                                                  <  2:214592/229‑1 (MQ=255)
                                                                                                                                gATTTTGTTAATAATTAAGTGGGTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCa                                                                                                           <  1:570837/251‑1 (MQ=255)
                                                                                                                                   tttGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAACGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGCATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCg                                                                                                        >  1:558147/1‑251 (MQ=255)
                                                                                                                                               tAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAACAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGa                                                                                             >  2:546206/1‑250 (MQ=255)
                                                                                                                                                           tggttAAAAATAATGCTGAGGTAATAAAAGTGTGAGAAGCGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAACTCGGTCCGGa                                                                                 >  2:194759/4‑250 (MQ=255)
                                                                                                                                                           tGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGa                                                                                 >  1:130368/1‑250 (MQ=255)
                                                                                                                                                                 aaaaTAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGACATCGGTCCGGAAAtcga                                                                           >  2:186548/1‑247 (MQ=255)
                                                                                                                                                                 aaaaTAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAAGGCGATTAACAGACACTCTTATTCGATTGACACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGaa                                                                          <  2:111793/251‑1 (MQ=255)
                                                                                                                                                                       atGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCg                                                                    >  1:52391/1‑251 (MQ=255)
                                                                                                                                                                          cTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGc                                                                  >  2:302277/1‑250 (MQ=255)
                                                                                                                                                                                      aaGTGTGGGAAGTTTATTACATTAAGTTATTTTGGCGTCAAGGCGATTAAAAGCCTCTCTTATTCAATTGCCACTCAGGTAGTATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCAGCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACgg                                                     <  2:477624/251‑1 (MQ=255)
                                                                                                                                                                                         tgtgtgAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGc                                                  >  1:274299/1‑251 (MQ=255)
                                                                                                                                                                                                 gtgtATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGa                                           >  1:217672/1‑250 (MQ=255)
                                                                                                                                                                                                  tgtATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGaa                                          >  2:7454/1‑250 (MQ=255)
                                                                                                                                                                                                   gtATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGaaa                                         >  1:435026/1‑250 (MQ=255)
                                                                                                                                                                                                    tATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAAt                                       >  2:481979/1‑251 (MQ=255)
                                                                                                                                                                                                        aCATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATacac                                   >  2:103776/1‑251 (MQ=255)
                                                                                                                                                                                                            tAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCa                                                                                                                                                                                       >  2:170915/1‑99 (MQ=255)
                                                                                                                                                                                                            tAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCa                                                                                                                                                                                       <  1:170915/99‑1 (MQ=255)
                                                                                                                                                                                                                   ttttGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGACATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGc                         >  1:77556/1‑250 (MQ=255)
                                                                                                                                                                                                                   ttttGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGCATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCATACGGTTCACGGTGCTCATCGAAAATACACGCTGCACGGc                         >  1:379480/1‑250 (MQ=255)
                                                                                                                                                                                                                       ggCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGt                    >  1:327376/1‑251 (MQ=255)
                                                                                                                                                                                                                        gCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTa                   <  1:7454/251‑1 (MQ=255)
                                                                                                                                                                                                                           tCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGcc                >  1:435884/1‑251 (MQ=255)
                                                                                                                                                                                                                            aaaTGCGATTAACAGACACTCTTATTATATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGccc               >  2:79952/2‑251 (MQ=255)
                                                                                                                                                                                                                               tGCGATTAAAAGACACTCTTATTCTGTTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCAGCCCGAAACAGTCAGCGCATTCAACCGTACGCCAGAGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGAGAACTCGATCCGGAAATAGAACTTGCGGCAAACGGTGCACGGTGCTCATCGAAAATACACGCTACAAGGCAACGGAGcccc              >  2:348347/1‑249 (MQ=255)
                                                                                                                                                                                                                                  gATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGc         >  2:343000/1‑251 (MQ=255)
                                                                                                                                                                                                                                       aCAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATc                                             >  2:179298/1‑210 (MQ=255)
                                                                                                                                                                                                                                       aCAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATc                                             <  1:179298/210‑1 (MQ=255)
                                                                                                                                                                                                                                         aGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTcc  >  1:218950/1‑251 (MQ=255)
                                                                                                                                                                                                                                         aGACACTCTTATTCTATTGACACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTcc  >  1:439819/1‑251 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    
TTATTGATGTCTTGAATTTTAACTATCAGAACAGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACCCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCC  >  NC_000913/4222504‑4222987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑