Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,179,001	C→T	S41S (AGC→AGT)	rplA →	50S ribosomal subunit protein L1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,179,001	0	C	T	100.0%	219.9 / NA	66	S41S (AGC→AGT)	rplA	50S ribosomal subunit protein L1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/29);  total (37/29)

GCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGCGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTT  >  NC_000913/4178754‑4179247                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       gcgcTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGTCCAAGCGCATGCTTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGtgtg                                                                                                                                                                                                                                                     <  1:369296/251‑1 (MQ=255)  cgcTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTAGTAGAAAGTGTgg                                                                                                                                                                                                                                                    >  2:22717/1‑251 (MQ=255)           gCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCt                                                                                                                                                                                                                                            <  2:221285/250‑1 (MQ=255)                 aaTCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAAcc                                                                                                                                                                                                                                     >  1:270062/1‑251 (MQ=255)                          gACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCg                                                                                                                                                                                                                            <  1:146451/251‑1 (MQ=255)                                           tGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGAc                                                                                                                                                                                                           >  1:301364/1‑251 (MQ=255)                                            gACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGAc                                                                                                                                                                                                           <  2:171539/250‑1 (MQ=255)                                                     cGACATTGAAGCGATGACTCGCTCCATCGAAGGTACAGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGACAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAacgtac                                                                                                                                                                                                 >  2:421663/1‑251 (MQ=255)                                                       aCATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAacgtacgt                                                                                                                                                                                               >  2:324876/1‑251 (MQ=255)                                                       aCATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAacgtacgt                                                                                                                                                                                               >  1:432793/1‑251 (MQ=255)                                                         aTTGAAGCGATGACTCGCTCCAGCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCAGCGACGCTCGTAAATCTGACCAGAacgtacgtag                                                                                                                                                                                             <  2:482948/251‑3 (MQ=255)                                                          ttGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACgtggg                                                                                                                                                                                            >  1:96033/1‑250 (MQ=255)                                                                  aTGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGAGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGt                                                                                                                                                                                    >  1:23655/1‑251 (MQ=255)                                                                     aCTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACt                                                                                                                                                                                 <  2:441237/251‑1 (MQ=255)                                                                     aCTCGCTCCATCGAAGGTACTGCACGGTCCATTGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCTGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGTTTCAACTGTACt                                                                                                                                                                                 <  1:479475/251‑1 (MQ=255)                                                                            ccATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGc                                                                                                                                                                            <  1:283166/249‑1 (MQ=255)                                                                             cATCGAATGTACTGCACGTTCCATGGGCCTTGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGAGGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGCCTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGTCCATAACGTACGTGGTGCAACTGTACTGCCGctcg                                                                                                                                                                         <  1:457417/251‑4 (MQ=255)                                                                             cATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACg                                                                                                                                                                         >  1:441174/1‑251 (MQ=255)                                                                             cATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGGGGTGCAACTGTACTGCCGCACg                                                                                                                                                                         <  1:169804/251‑1 (MQ=255)                                                                                cGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGt                                                                                                                                                                       >  1:550904/1‑250 (MQ=255)                                                                                   aGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCACCTGTACTGCCGCACGGTACTg                                                                                                                                                                   >  2:106279/1‑251 (MQ=255)                                                                                    ggTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTgg                                                                                                                                                                  <  2:437043/251‑1 (MQ=255)                                                                                           cACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGccgttcc                                                                                                                                                           <  2:108479/251‑1 (MQ=255)                                                                                            aCGTTCGATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACTACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGccgttccg                                                                                                                                                          <  2:530013/251‑1 (MQ=255)                                                                                                tCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACTTGGTGCAACTGTACTGCCGCACGGTACTGGCcgttccgttc                                                                                                                                                       <  2:486379/250‑1 (MQ=255)                                                                                                  cATGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGAGTGTTATCCGCGAGAAAGATGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGTCGAATGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTAATGCCGCACGGTACTGGCCGGTCCGTTcgcg                                                                                                                                                    >  2:454647/1‑251 (MQ=255)                                                                                                    tGGGCCTGGTAGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGt                                                                                                                                                   >  1:215436/1‑250 (MQ=255)                                                                                                              aGTGGAGGACTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACCTCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAttt                                                                                                                                         >  1:216776/1‑250 (MQ=255)                                                                                                                     gaCTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAgg                                                                                                                                 <  2:432793/251‑1 (MQ=255)                                                                                                                       cTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCACGGTg                                                                                                                               >  2:541518/1‑251 (MQ=255)                                                                                                                         aaGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGc                                                                                                                              >  1:548956/1‑250 (MQ=255)                                                                                                                                     aaCTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAgct                                                                                                                 <  2:324101/251‑1 (MQ=255)                                                                                                                                      aCTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGAGCAAACGCTGAAgctg                                                                                                                >  2:359447/1‑251 (MQ=255)                                                                                                                                      aCTGACCAAGCGCATGCGCGGTATCCGCGAGACAGTAGATGCAACCAAGCAGTACGACATCGACGAAGCTATCGCACTGCTGAAAGAGCTAGCAAGTGCTAAATTCGTAGACAGTGTGGGCGTAGCTGTTAACCTCGGCATCGACGCTCGTCAATATGACCAGAACATACGTGGTGCAACTGTACTGCCGCAGGGTGCTGGCCGTTCCGGTCGCGGGGCCGTATTTACCAAAGGTGGAAAAGCGGAAgagg                                                                                                                >  2:465925/1‑248 (MQ=255)                                                                                                                                            cAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTaa                                                                                                            >  1:338287/1‑249 (MQ=255)                                                                                                                                             aaGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGc                                                                                                         <  1:145569/251‑1 (MQ=255)                                                                                                                                              aGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGGGCAAACGCTGAAGCTGCTAAAGcg                                                                                                        >  1:172507/1‑250 (MQ=255)                                                                                                                                                  cATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGc                                                                                                      >  1:341359/1‑249 (MQ=255)                                                                                                                                                              ccGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGAATGCTAAATTCGTAGACAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTgg                                                                                        >  1:379938/1‑251 (MQ=255)                                                                                                                                                                  gagaAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTggtagg                                                                                    >  2:295576/1‑251 (MQ=255)                                                                                                                                                                   agaAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTggtaggt                                                                                   >  1:411121/1‑251 (MQ=255)                                                                                                                                                                          tGATGCAACCAAACAGTACGACATCAACGACGCTATCGCACTGCTGAAAGAGATGGCGACTGCTAAATTAGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTCAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTggtaggactgga                                                                              >  1:583054/1‑243 (MQ=255)                                                                                                                                                                          tGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGa                                                                              >  1:147359/1‑249 (MQ=255)                                                                                                                                                                               cAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTg                                                                       <  2:96033/251‑1 (MQ=255)                                                                                                                                                                                aaCCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTgg                                                                      >  2:226506/1‑251 (MQ=255)                                                                                                                                                                                   cAAACAGTACGACATCACCGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTTGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCTCGTAGCCGTATTTACCCAAGGTGCAAACGCGGAAGCTGCTAGAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTg                                                                   <  1:59049/251‑1 (MQ=255)                                                                                                                                                                                                   aaCGAAGCTATCGCACGGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCTTAAATCTGACCAGAACGTACGTGGGGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCACACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGCTCACGAAAgg                                                   >  2:142598/1‑251 (MQ=255)                                                                                                                                                                                                     cGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCACGAAAGGCg                                                 >  2:219679/1‑251 (MQ=255)                                                                                                                                                                                                      gAAGCTAGCGCACTGCTGAAAGAGCTGGAGACTGCTAAATTCGTAGAAAGTGTGGCCGTAGCTGTTAACCTCGGCATCGACGCGCGTAAATCTGACCAGAATGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCGGCAGGCGCAGAACTGGTAGGGATGGAAGATCTGGTGGACCAGATCAAGAAAGGCGa                                                <  2:582838/251‑1 (MQ=255)                                                                                                                                                                                                         gCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAAt                                             >  2:485178/1‑251 (MQ=255)                                                                                                                                                                                                              cggaCTGCTGAAAGAGCTGTCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACt                                        <  2:441609/248‑1 (MQ=255)                                                                                                                                                                                                              cGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGGCCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTTCTAAAGCTGCAGGCGCAGAACTGGTATGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACt                                        <  2:338287/251‑1 (MQ=255)                                                                                                                                                                                                               gCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACtt                                       <  2:435602/251‑1 (MQ=255)                                                                                                                                                                                                               gCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACtt                                       >  2:437922/1‑251 (MQ=255)                                                                                                                                                                                                                       gAAATAGCTGGCTGCTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCACGGCATCGACGCTCGAAAAACTGCCCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTGCCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGCACTTTGACgtt                                <  2:324112/250‑1 (MQ=255)                                                                                                                                                                                                                       gAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACgtt                                <  2:311420/250‑1 (MQ=255)                                                                                                                                                                                                                       gAAAGAGCTGGCGACTGCTAAATACGTAGAAAGTGTGGACGTAGCTGTGAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTGTGACgttg                               <  2:216776/251‑1 (MQ=255)                                                                                                                                                                                                                            agCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTAtt                          >  1:270796/1‑251 (MQ=255)                                                                                                                                                                                                                              cTGGCGACTGATAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGAATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCCCGGTACTGGCCGTTCCGTTCGCGTAGCCGTCTTTACCCATGGTGCAAACGCAGACGCTGCTATAGCTGCAGGCGCAGAACAGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGCAAGGCGAAATGAACTTTGACGTTGTTATTGc                        >  1:361104/1‑251 (MQ=255)                                                                                                                                                                                                                                ggCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATTGACGCTCGTAAATTTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTTGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCtt                      <  1:294496/251‑1 (MQ=255)                                                                                                                                                                                                                                     cTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCCAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCg                 >  2:402890/1‑251 (MQ=255)                                                                                                                                                                                                                                     cTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCg                 >  2:138130/1‑251 (MQ=255)                                                                                                                                                                                                                                      tGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCgg                >  2:491614/1‑251 (MQ=255)                                                                                                                                                                                                                                       gCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTACCCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGa               <  2:441174/251‑1 (MQ=255)                                                                                                                                                                                                                                             ttCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGCTGCAAt         >  1:116180/1‑251 (MQ=255)                                                                                                                                                                                                                                                  aGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGcgcg    <  2:202803/251‑1 (MQ=255)                                                                                                                                                                                                                                                    aaaGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCgtt  <  1:226506/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       GCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGCGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATTTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTT  >  NC_000913/4178754‑4179247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑