Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,950,099	C→T	intergenic (‑130/‑223)	yifB ← / → ilvL	putative magnesium chelatase YifB/ilvXGMEDA operon leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,950,099	0	C	T	96.3%	70.7 / NA	27	intergenic (‑130/‑223)	yifB/ilvL	putative magnesium chelatase YifB/ilvXGMEDA operon leader peptide
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (10/16);  minor base G (0/1);  total (10/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

GTAAGCCCACCATCGTTAAGCCGGGTAGACCTTTACTGATATGTACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGAT  >  NC_000913/3949858‑3950344                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      gTAAGCCCACCATCGTTAAGCCGGGTAGACCTTTACTGATATGTACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCaaaa                                                                                                                                                                                                                                               >  1:177412/1‑250 (MQ=255)                  aaGCCGGGTAGACCTTTACTGATATGTACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGAt                                                                                                                                                                                                                             <  2:13012/251‑1 (MQ=255)                                       atatGTACCTCAACAGAGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGaaca                                                                                                                                                                                                         >  1:409950/1‑250 (MQ=255)                                        tatGTACCTAAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCACTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTAAGAGGAAGGGaaaaac                                                                                                                                                                                                       >  2:92412/1‑251 (MQ=255)                                             aCCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTTGAATGATGGTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCa                                                                                                                                                                                                  <  2:94393/251‑1 (MQ=255)                                             aCCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCTCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCa                                                                                                                                                                                                  <  1:226132/251‑1 (MQ=255)                                                  aaCAGTGATCGGGGGCGCATTTTCTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCCTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTg                                                                                                                                                                                             <  2:327933/251‑1 (MQ=255)                                                   aCAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTCTGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGACTTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGATTGATGTTCGCAAATATGCAGCGGACAAAGGATGCACTACGAGGAAGGGAACAACATTCATACTg                                                                                                                                                                                             >  1:565814/1‑250 (MQ=255)                                                                        aCTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTtcactca                                                                                                                                                                        <  1:25445/250‑1 (MQ=255)                                                                                       cgGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTaaa                                                                                                                                                        <  1:282854/251‑1 (MQ=255)                                                                                         ggTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTaaaaa                                                                                                                                                      <  1:142253/251‑1 (MQ=255)                                                                                                aCAATTGACAGTGACATAAGCCCTCCGTGAGTCACCATTATGTGCATAAGATATCGCTTCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAcaacaa                                                                                                                                               <  1:398955/251‑1 (MQ=255)                                                                                                   aTTGACAGTGACATAAGCCCTCCGTGAGTCTCCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATGCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATTAACTACGAGGAAGGTAACAACATTCATACTGAAATTGAATTTGTTTCACTCACTATTTTATTTTTAAAAAACAACAAttt                                                                                                                                            <  2:334631/251‑1 (MQ=255)                                                                                                     tGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTCATTCGTGAATTTTAGTGGCTGATTTCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTACTAACTATTTTATTTTTAAAAAAAAACAATTtat                                                                                                                                          >  1:388716/1‑251 (MQ=255)                                                                                                        cAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATAtt                                                                                                                                       <  1:4697/251‑1 (MQ=255)                                                                                                                        cctTGAGTCACCATTATGTGCATACGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGACTGATGTTTGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGGAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATTTTGCAATTATTAAACGc                                                                                                                        >  2:126480/1‑250 (MQ=255)                                                                                                                                       aTGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTTAATTTTAGTGGCTGATTCCTGCTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGAGGAACTACGAGGAAGGGAACAACATTCATAGTGAAATTGAATTTTTTTCACTCACTATTTTATTGTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGcc                                                                                                        <  1:92412/251‑1 (MQ=255)                                                                                                                                         gtgCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAAGCGGCCa                                                                                                       <  1:434094/250‑1 (MQ=255)                                                                                                                                                                  ccGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACaaa                                                                              >  2:422141/1‑250 (MQ=255)                                                                                                                                                                   cGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAAGCGGCCAAAAAATATCTTGTACTATTTACAAAAc                                                                            <  2:79393/251‑1 (MQ=255)                                                                                                                                                                                     aGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAACATGCAGCTGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTCATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAgg                                                          <  1:541100/251‑1 (MQ=255)                                                                                                                                                                                                             gCAAGTGCAATTGACTTGTTCTAGCGGTGGCCTGCTGTTCGCCAAAATGCCGCCGACAAAGGATGACCTACGAGGAAGGGAACAACCTTCCTAATGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAAAAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTTTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGAtaca                                     >  2:252963/1‑245 (MQ=255)                                                                                                                                                                                                                     agttgagttgTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACaa                          <  2:213991/251‑1 (MQ=255)                                                                                                                                                                                                                     agttgagttgTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACGCGTTAGGCATTCCTGCGTACAAGATGCAAGAAAAGACaa                          <  2:565814/251‑1 (MQ=255)                                                                                                                                                                                                                             tgttCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTAACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACa                   <  1:302466/250‑1 (MQ=255)                                                                                                                                                                                                                             tgttCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAAATACGAGGAAGGGAAAAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAAAAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTAAAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAACAGACAAAATGAc                    >  2:21267/1‑249 (MQ=255)                                                                                                                                                                                                                                             tgatgTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGAt  >  1:500253/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      GTAAGCCCACCATCGTTAAGCCGGGTAGACCTTTACTGATATGTACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGAT  >  NC_000913/3949858‑3950344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑