Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	312,865	G→A	intergenic (‑88/+276)	ykgM ← / ← ykgR	50S ribosomal subunit protein L31B/putative membrane protein YkgR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	312,865	0	G	A	100.0%	109.2 / NA	35	intergenic (‑88/+276)	ykgM/ykgR	50S ribosomal subunit protein L31B/putative membrane protein YkgR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/18);  total (17/18)

TTTAGAAGAGACATCAATTGTCACGTATGGATACGTTACGCCATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAA  >  NC_000913/312622‑313109                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     tttAGAAGAGACATCAATTGTCACGTATGGATACGTTACGCCATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTtgtgaatgtgg                                                                                                                                                                                                                                                >  1:317780/1‑250 (MQ=255)    agaagaGACATCAATTGTCACGTATGGATACGTTACGCCATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTAATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAAtt                                                                                                                                                                                                                                            <  2:125221/251‑1 (MQ=255)                   tGTCACGTATGGATACGTTACGCCATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGCCTCTTTGTGAATGTGGAATTTTCGCAACGGGGAAc                                                                                                                                                                                                                             >  2:140499/1‑251 (MQ=255)                           aTGGATACGTTACGACATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCCCAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGACCTCTGGCAt                                                                                                                                                                                                                     >  2:528031/1‑251 (MQ=255)                             ggATACGTTACGCCATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACAGGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCAtt                                                                                                                                                                                                                    <  2:516944/250‑1 (MQ=255)                               aTACGTTACGCCATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGtt                                                                                                                                                                                                                 >  2:300552/1‑251 (MQ=255)                                tACGTTACGCCATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGtt                                                                                                                                                                                                                 >  1:494028/1‑250 (MQ=255)                                         ccagccAGCTCAATCTCACGGTCTGTTTTGATAGCCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGTAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCAtt                                                                                                                                                                                                       <  1:151902/247‑1 (MQ=255)                                          cATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGCACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCACCGGGGAACTCTGGCATTGTTAGCAGCCATTg                                                                                                                                                                                                      >  1:398217/1‑251 (MQ=255)                                                              tCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGcc                                                                                                                                                                                  >  1:26800/1‑251 (MQ=255)                                                                            cGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGtgatt                                                                                                                                                                     <  1:300552/250‑1 (MQ=255)                                                                                                   caACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGGTTGATGATGGTTTTGCTGAGAGTATg                                                                                                                                             >  1:224702/1‑251 (MQ=255)                                                                                                           gtgtCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTc                                                                                                                                     <  1:120732/251‑1 (MQ=255)                                                                                                            tgtCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGCGTATGGATTTTTCg                                                                                                                                    >  2:154289/1‑251 (MQ=255)                                                                                                                                                   tGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACTCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGTCCGATAAttt                                                                                              <  2:317780/250‑1 (MQ=255)                                                                                                                                                   tGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAAttt                                                                                              >  1:303121/1‑250 (MQ=255)                                                                                                                                                   tGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAAttt                                                                                              >  2:167297/1‑250 (MQ=255)                                                                                                                                                         tcatcaTTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGc                                                                                         <  1:140499/249‑1 (MQ=255)                                                                                                                                                                ttttACCTGTTATGTTATAACATAACCATAAAGATACATGCTCAACCGGATCATTTCAACACTCAATCCATGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTAtgat                                                                                 <  2:21516/250‑1 (MQ=255)                                                                                                                                                                      cTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTAc                                                                          >  1:124676/1‑251 (MQ=255)                                                                                                                                                                             gttatAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGGTTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACa                                                                    <  1:532566/250‑1 (MQ=255)                                                                                                                                                                                       aacCATAAAGATACATGCTCATCCGGATCATTTCAGCACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGAAGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGCTAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTcc                                                         >  2:516295/1‑251 (MQ=255)                                                                                                                                                                                            tAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGATAGCGTGTATTCCTCTcc                                                    >  2:368530/1‑251 (MQ=255)                                                                                                                                                                                                gATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACg                                                <  2:142234/251‑1 (MQ=255)                                                                                                                                                                                                 aTACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACgg                                               <  1:209651/251‑1 (MQ=255)                                                                                                                                                                                                  tACATGCTCATCCGGATCATTCCAAGACTCAATCCCTATGACTCTTAGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGGGGTTATCTCAACTTTACTGGCCGATAATTTATCGCAGTATGCTTGCTTACCTGACAGCGTGTATTCCTCTCCCACgg                                               >  2:76103/1‑250 (MQ=255)                                                                                                                                                                                                    cATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTa                                                             >  1:32173/1‑234 (MQ=255)                                                                                                                                                                                                    cATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGAGTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTa                                                             <  2:32173/234‑1 (MQ=255)                                                                                                                                                                                                            accGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAAtt                                    <  1:180438/250‑1 (MQ=255)                                                                                                                                                                                                             ccGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAAtta                                   <  1:528031/251‑1 (MQ=255)                                                                                                                                                                                                              cGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGAATGTGGACTTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAAtta                                   >  2:266299/1‑250 (MQ=255)                                                                                                                                                                                                                           cacTCAATCCCTGTGACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAggg                     <  1:148360/251‑1 (MQ=255)                                                                                                                                                                                                                                       gtgACTGTTTGAGAATGTGGAATTTTCGCAACGGCGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTGTGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTCTCGCAGGATGATTGATTACCTGACAGCGTGTATTACTCTCCTACGGATATAATAATTACTTACATAAAAGGGGGAGGAAGCat          >  2:112346/1‑250 (MQ=255)                                                                                                                                                                                                                                       gtgACTCTTTGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCat          <  1:167297/250‑1 (MQ=255)                                                                                                                                                                                                                                               ttGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGATAGGATGCATATTTTaaa  <  1:428719/250‑1 (MQ=255)                                                                                                                                                                                                                                               ttGTGAATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTaa   <  2:144460/249‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     TTTAGAAGAGACATCAATTGTCACGTATGGATACGTTACGCCATCCAGCTCAATCTCACGGTCTGTTTTGATAGTCGAGCCGATTTTAAAGTACTCATCAACACTGGTGTCGTGGAACACCACAGTACGATACTCAGGATGGATATTGGGCTTCATCATTTTTACCTGTTATGTTATAACATAACCATAAAGATACATGCTCATCCGGATCATTTCAACACTCAATCCCTGTGACTCTTTGTGGATGTGGAATTTTCGCAACGGGGAACTCTGGCATTGTTAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAA  >  NC_000913/312622‑313109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑