Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,870,464 C→T V423M (GTG→ATG)  dgoT ← D‑galactonate:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,870,4640CT98.6% 234.1 / NA 69V423M (GTG→ATG) dgoTD‑galactonate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (42/26);  minor base G (0/1);  total (42/27)
Fisher's exact test for biased strand distribution p-value = 3.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCTCGCTGGAGGGGATTAGCTATGCGCTGGATAGCACAGACATTCTGCGTTCGGTGTTACTGAAGCAGCCAGAGAAGCTCAATACCGCTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGACCAGGTAATCGAAGCAAAACCGTTACCGAAGAATGCCAGCGCCATCA  >  NC_000913/3870217‑3870703
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               
aGCTCGCTGGAGGGTATTAGCTATGCGCTGGATAGCACAGACATTCTGCGTTCGGTGTTACTGAAGCAGCCAGAGAAGCTCAATACCGCTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATcag                                                                                                                                                                                                                                              <  2:372100/251‑1 (MQ=255)
         gAGGGGATTAGCTATGCGCTGGATAGCACAGACATTCTGCGTTCGGTGTTACTGAAGCCGCCAGAGAAGCTCAATACCGCTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGAACGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGatat                                                                                                                                                                                                                                      >  2:52035/1‑250 (MQ=255)
         gAGGGGATTAGCTATGCGCTGGATAGCACAGAAATTCTGCGTTCGGTGTTACTGAAGCAGCCAGAGAAGCTCAATACCGCTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGCTTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAAAGCGCTTCACATCGCCCATCAGCAGGatat                                                                                                                                                                                                                                      >  2:340803/1‑250 (MQ=255)
                  aGCTATGCGCTGGATAGCACAGACATTCTCCGTTCGGTGTTACTGAAGCAGCCAGAGAAGCTCAATACCGCTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAgcgc                                                                                                                                                                                                                            >  1:410576/1‑251 (MQ=255)
                          gcTGGATAGCACAGACATTCTGCGTTCGGTGTTACTGAAGCAGCCAGAGAAGCTCAATACCGCTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCa                                                                                                                                                                                                                    <  2:220188/251‑1 (MQ=255)
                            gggATAGCACAGACATTCTGCGTTCGGTGTTACTGAAGCAGCCAGAGAAGCTCAATACCGCTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAAc                                                                                                                                                                                                                  <  2:509258/250‑1 (MQ=255)
                                   cacaGACATTCTGCGTTCGGTGTTACTGAAGCAGCCAGAGAAGCTCAATACCGCTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcgg                                                                                                                                                                                                           >  2:263805/1‑251 (MQ=255)
                                   cacaGACATTCTGCGTTCGGTGTTACTGAAGCAGCCAGAGAAGCTCAATACCGCTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcgg                                                                                                                                                                                                           >  1:39174/1‑251 (MQ=255)
                                    acaGACATTCAGCGTTCGGTGTTACTGAAGCAGCCAGAGAAGCTCAATACCGCTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGCTTAGCCACCGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGAGCGCCACTCAACGCGCcggc                                                                                                                                                                                                          >  2:355378/1‑251 (MQ=255)
                                           ttCTGCGTTCGGTGTTACTGAAGCAGCCAGAGAAGCTCAATACCGCTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGat                                                                                                                                                                                                    >  2:215077/1‑250 (MQ=255)
                                               gCGTTCGGTGTTACTGAAGCAGCCAGAGAAGCTCAATACCGCTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATaaa                                                                                                                                                                                               <  2:508092/251‑1 (MQ=255)
                                                     ggtggtACTGAAGCAGCCAGAGAAGCTCAATACCGCTTACGGGCTCGCCACCCGCAAACTGCGTTTAAAACGCTACGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTGGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCATCGCGACGGCGGAGATATAAACCAGTg                                                                                                                                                                                         <  2:111580/251‑1 (MQ=255)
                                                        gtTACTGAAGCAGCCAGAGAAGCTCAATACCGCTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAg                                                                                                                                                                                      <  1:192152/251‑1 (MQ=255)
                                                          tACTGAAGCAGCCAGAGAAGCTCAATACCGCTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGt                                                                                                                                                                                    >  2:100751/1‑251 (MQ=255)
                                                              gAAGCAGCCAGAGAAGCTCAATACCGCTTACTGGCGCGCCACCCGCAAACTGCGTTTAAACCTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGAGTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCATCGCGACGGCGGAGATATAAACCAGTGCAGGTGCg                                                                                                                                                                                 <  1:313994/250‑1 (MQ=255)
                                                                     ccAGAGAAGCTCAATACCGCTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGt                                                                                                                                                                          >  2:227381/1‑250 (MQ=255)
                                                                     ccAGAGAAGCTCAATACCGCTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTa                                                                                                                                                                         <  1:139806/251‑1 (MQ=255)
                                                                           aaGCTCAATACCGCTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTg                                                                                                                                                                   >  2:459256/1‑251 (MQ=255)
                                                                             gCTCAATACCGCTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTgcg                                                                                                                                                                 >  1:47855/1‑251 (MQ=255)
                                                                                 aaTACCGCTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGCTGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCACCGCGACGGCGGAGATCTAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGcc                                                                                                                                                               >  1:553617/1‑249 (MQ=255)
                                                                                     ccGCTTACTGGCGAGCCGCCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTCACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAACCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTAc                                                                                                                                                         <  2:451908/251‑1 (MQ=255)
                                                                                        cTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTAcccc                                                                                                                                                      >  1:474925/1‑251 (MQ=255)
                                                                                         ttACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAAGTGCGAAACCGTAACCCTGCGCCAGGTAcccc                                                                                                                                                      >  2:14558/1‑250 (MQ=255)
                                                                                           aCTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCccacc                                                                                                                                                   <  1:96293/251‑1 (MQ=255)
                                                                                                   gcCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCgg                                                                                                                                           >  1:503129/1‑251 (MQ=255)
                                                                                                    ccACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCgg                                                                                                                                           >  1:148146/1‑250 (MQ=255)
                                                                                                      aCCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGACAGGTACCCCACCACCAGCGGAAc                                                                                                                                        >  1:392438/1‑251 (MQ=255)
                                                                                                              aCTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATgc                                                                                                                                >  2:193903/1‑251 (MQ=255)
                                                                                                                  cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATgccgcc                                                                                                                            >  1:41521/1‑251 (MQ=255)
                                                                                                                   gTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGCTGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCACTCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGCTGCCGccc                                                                                                                           >  1:212833/1‑251 (MQ=255)
                                                                                                                        aaaCTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGacc                                                                                                                      <  1:193903/251‑1 (MQ=255)
                                                                                                                                  gCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAg                                                                                                            <  1:497210/251‑1 (MQ=255)
                                                                                                                                     aGATAGTAAAAAGACGATGCCTGACCGCACCGGCTGTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGGAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGGGATGCCGCCCATACCACCGGCGAATTTa                                                                                                         <  2:339552/251‑1 (MQ=255)
                                                                                                                                      gATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTa                                                                                                         >  1:213226/1‑250 (MQ=255)
                                                                                                                                      gATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGAGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGAGATGCCGCCCAGACCACCGGCGAAGTTa                                                                                                         >  2:575406/1‑250 (MQ=255)
                                                                                                                                                   cGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACAGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGt                                                                                           >  1:127534/1‑251 (MQ=255)
                                                                                                                                                    gATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGtt                                                                                          >  1:178596/1‑251 (MQ=255)
                                                                                                                                                           gACCGCACCGGCTTGAAGAAAGTGGAGTATGCGCAGTGGTGTGGCACATAGTCCACAGTTGAAGGATTAGGCAACGCGCTTCACATCGCCCATCATCAGTATATAAGAGAGCGCTCCAAGCAACGCGACGGCGGAGATAGAAACCAGGGCATGTGCGAAACCGTCACCCTTCGCCAGTTACCCCACCACCTGCGGAACGGTGATGCCGCCCAGACCACCGGCGAATTTAAACACGCCCCCGGTTAACCCaa                                                                                   <  2:573034/251‑1 (MQ=255)
                                                                                                                                                            aCCGCACCGGCTTTAAGAAAGAGGATTATGCGCAGTGGTGTGGCACATATTACACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGAAGGCTATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACAAGTGCAGGCGCGAAACCGTAACCCTGCGCCAGGTACCCCAACACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGACGTTAAACACGCCGCCGGTTAAAACAAt                                                                                  >  1:285966/1‑251 (MQ=255)
                                                                                                                                                                 aCCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGgc                                                                             <  1:109201/251‑1 (MQ=255)
                                                                                                                                                                    ggCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCATGCGCa                                                                          <  2:536432/251‑1 (MQ=255)
                                                                                                                                                                        ttAAGAAAGTGGATTATGCGCAGTGGTGGGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCg                                                                       <  2:474925/250‑1 (MQ=255)
                                                                                                                                                                        ttAAGAAAGTGGATTATGCGCAGTGGCGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACCTCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCgg                                                                      >  1:336024/1‑251 (MQ=255)
                                                                                                                                                                         tAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGt                                                                     >  1:498858/1‑251 (MQ=255)
                                                                                                                                                                          aaGAAAGTGGATGATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGCTTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAAGCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTg                                                                    <  1:406765/251‑1 (MQ=255)
                                                                                                                                                                           aGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATAGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGAGCGCAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGAGATGCCGCACAGACAACCGGCGAAGTTAAACACGCCGCCGGGTAAACCACTCAGGCGCATCggcgc                                                                   >  1:344506/1‑248 (MQ=255)
                                                                                                                                                                              aaGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGcc                                                                  >  1:188801/1‑249 (MQ=255)
                                                                                                                                                                                  ggATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGCCGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTCACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACAGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAgaga                                                             >  1:349688/1‑250 (MQ=255)
                                                                                                                                                                                  ggATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACCCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAgaga                                                             >  1:195989/1‑250 (MQ=255)
                                                                                                                                                                                      tATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAGAgaagaa                                                         <  1:100751/250‑1 (MQ=255)
                                                                                                                                                                                             agtggagtggCACATCTTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGCTATAAGAGAGCGCGCCAATCAACGCGAAGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGCACGGTGATGCCGCCCAGACCACCGGCGCAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAGAGAAGAAACCAGCg                                                  >  2:540533/1‑250 (MQ=255)
                                                                                                                                                                                                ggtgTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGACCa                                              <  1:52035/251‑1 (MQ=255)
                                                                                                                                                                                                   gtgGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGACCAGGt                                           >  1:231642/1‑251 (MQ=255)
                                                                                                                                                                                                      gCACATATTCCACAGTTGACGGATTAGCCAACGCGCTTCACGTCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGCGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGCCCAGGAAAt                                        >  2:315703/1‑251 (MQ=255)
                                                                                                                                                                                                           tatTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCACTCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGACAAGGTAATCGa                                     >  1:69290/1‑249 (MQ=255)
                                                                                                                                                                                                           tatTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGACCAGGTAATCGa                                     >  2:238655/1‑249 (MQ=255)
                                                                                                                                                                                                             ttCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAg                                                                                                                                              <  2:202357/142‑1 (MQ=255)
                                                                                                                                                                                                             ttCCACAGTTGAAGGATTAGCCAACGCGCATCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGGGCGACACCGTAACCCTGCGCCAGGTACCCCACCACCAg                                                                                                                                              >  1:202357/1‑142 (MQ=255)
                                                                                                                                                                                                                      tGAAGGATTAGCCAACGCGCTTCACATCGCCCAGAAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGACCAGGTAATCGAAGCAAAACCGtt                         <  2:47855/250‑1 (MQ=255)
                                                                                                                                                                                                                          ggATTAGCCAACGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCCTCGGTGCCAGAGAAGAAACCAGCGACCAGGTAATCGAAGCAAAACCGTTACCg                     >  1:542906/1‑250 (MQ=255)
                                                                                                                                                                                                                                   aaCGCGCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGACCAGGTAATCGAAGCAAAACCGTTACCGAAGAATGCCa           <  2:213226/251‑1 (MQ=255)
                                                                                                                                                                                                                                     cgcgCTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTTCAGGTGCGAATCCGTAACCCTGCGCCAGGTAACCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGAGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGACCAGGTAATCGAAGCAAAACCGTTACCGAAGAATGCCAgc         <  1:541678/251‑1 (MQ=255)
                                                                                                                                                                                                                                       cgcTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGCAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGACCAGGTAATCGAAGCAAAACCGTTACCGAAGAATGCCAgcgc       >  2:289617/1‑251 (MQ=255)
                                                                                                                                                                                                                                        gcTTCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGACCAGGTAATCGAAGCAAAACCGTTACCGAAGAATGCCAGCGcc      >  1:86987/1‑251 (MQ=255)
                                                                                                                                                                                                                                           tcgcaTCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAGCCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAAGCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGACCAGGTAATCGAAGCAAAACCGTTACCGAAGAATGCCAGCGCCa     <  2:39174/246‑1 (MQ=255)
                                                                                                                                                                                                                                           tCACATCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGACCAGGTAATCGAAGCAAAACCGTTACCGAAGAATGCCAGCGCCATc   >  1:7798/1‑251 (MQ=255)
                                                                                                                                                                                                                                            cacaTCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCTGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGACCAGGTAATCGAAGCAAAACCGTTACCGAAGAATGCCAGCGCCATCa  <  1:355378/251‑1 (MQ=255)
                                                                                                                                                                                                                                            cacaTCGCCCATCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGACCAGGTAATCGAAGCAAAACCGTTACCGAAGAATGCCAGCGCCATc   <  2:148146/250‑1 (MQ=255)
                                                                                                                                                                                                                                             acaTCGCCCATCAGCAGGATATCAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGACCAGGTAATCGAAGCAAAACCGTTACCGAAGAATGCCAGCGCCATc   >  1:229315/1‑249 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               
AGCTCGCTGGAGGGGATTAGCTATGCGCTGGATAGCACAGACATTCTGCGTTCGGTGTTACTGAAGCAGCCAGAGAAGCTCAATACCGCTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGACCAGGTAATCGAAGCAAAACCGTTACCGAAGAATGCCAGCGCCATCA  >  NC_000913/3870217‑3870703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑