Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,803,945	C→T	G65S (GGT→AGT)	waaB ←	UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,803,945	0	C	T	100.0%	77.8 / NA	24	G65S (GGT→AGT)	waaB	UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/11);  total (13/11)

CATTTGCTGTTTAATGCCAGAGCTGATTGCTAAATGATAGTCGGCACAGGTGATATATTCAGCATGTTTTTTGTGATCTAAAGAAAAATGTGGCCATGAAAATACTGGCATATCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGG  >  NC_000913/3803700‑3804178                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          cATTTGCTGTTTAATGCCAGAGCTGATTGCTAAATGATAGTCGGCACAGGTGATATATTCAGCATGTTTTTTGTGATCTAAAGAAAAATGTGGCCATGAAAATACTGGCATATCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAAc                                                                                                                                                                                                                                      <  1:522678/251‑1 (MQ=255)                                          cGGCACAGGTGATATATTCAGCATGTTTTTTGTGATCTAAAGAAAAATGTGGCCATGAAAATACTGGCATATCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACTCAAAAAACTTAAACGAATAGTGCAAAATGAACATTAGTATATAATACCTTCCAg                                                                                                                                                                                              <  1:433850/250‑1 (MQ=255)                                                    gATATATTCAGCATGTTTTTTGTGATCTAAAGAAAAATGTGGCTATGAAAATACTGGCATATCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTCCTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTa                                                                                                                                                                                    <  1:131135/250‑1 (MQ=255)                                                           tCAGCATGTTTTTTGTGATCTAAAGAAAAATGTGGCCATGAAAATACTGGCATATCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCAttt                                                                                                                                                                             <  1:37461/250‑1 (MQ=255)                                                             aGCATGTTTTTTGTGATCTAAAGAAAAATGTGGCCATGAAAATACTGGCATATCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGt                                                                                                                                                                          >  2:419635/1‑251 (MQ=255)                                                                    tttttGTGATCTAAAGCAAAATGTGGCCATGAAAATACTGGCATATCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATCTCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATACATTTTGTCATTACg                                                                                                                                                                   >  2:477807/1‑251 (MQ=255)                                                                        tgtgATCTAAAGAAAAATGTGGCCATGAAAATACTGGCATATCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACa                                                                                                                                                                <  2:271005/250‑1 (MQ=255)                                                                                 aaGAAAAATGTGGCCATGAAAATACTGGCATATCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAAc                                                                                                                                                      >  1:8314/1‑251 (MQ=255)                                                                                                         cTGGCATATCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAACAGAAAAACATTTCACTTTGGATATGTTGTTGc                                                                                                                              >  1:79700/1‑251 (MQ=255)                                                                                                                 tCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGgt                                                                                                                      >  1:322529/1‑251 (MQ=255)                                                                                                                   aaTTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGACTATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGgt                                                                                                                      >  1:74126/1‑249 (MQ=255)                                                                                                                                              ggCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTcc                                                                                          >  1:411007/1‑250 (MQ=255)                                                                                                                                                cAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATg                                                                                       >  1:25160/1‑251 (MQ=255)                                                                                                                                                 aaaCAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCATCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTGGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATgc                                                                                      <  2:367718/251‑1 (MQ=255)                                                                                                                                                               tCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGa                                                                         >  1:318711/1‑250 (MQ=255)                                                                                                                                                                      aaTACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCtt                                                                 <  2:318711/251‑1 (MQ=255)                                                                                                                                                                            aaTAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCAATGCCGCCAAATCCAGAAACAGCTTCGCCg                                                            >  1:543725/1‑250 (MQ=255)                                                                                                                                                                                   atatCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGc                                                    <  1:19166/251‑1 (MQ=255)                                                                                                                                                                                         ggttggtACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATGGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTAttt                                               <  2:411007/250‑1 (MQ=255)                                                                                                                                                                                                     tGAAGACAATTACTTAGAACATGAATATGCTTCGCCCGAAGCAAAAAACTTCAACGAATCTTGGAGACTGAACAAAAGTCTTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTAACTTTGGATATGTTGTTGCCTGACGGTAGTAATAACATCACGGATGAAAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTCTTTTAATAGACActct                                  >  2:523796/1‑251 (MQ=255)                                                                                                                                                                                                        aGCCATTTACTTAGAGCATCAATATGTCTCGCCCGACGCAAACAACTTACACGAATATTGGAAAATGCACATGCGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCCCTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATACACGCTATTTTCATAGAAACTCTCAt                               >  2:243802/1‑251 (MQ=255)                                                                                                                                                                                                                aCTTAGAGCATGAATAGGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGAGAAACGCTATTTTCATAGAAACTCTCATTAATTGa                        <  2:74126/250‑1 (MQ=255)                                                                                                                                                                                                                         aTGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATGTTCATAGAAACTCTCATTAATTGAGTAAAACTCa              <  2:25160/251‑1 (MQ=255)                                                                                                                                                                                                                                     cGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACgg  >  2:151211/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          CATTTGCTGTTTAATGCCAGAGCTGATTGCTAAATGATAGTCGGCACAGGTGATATATTCAGCATGTTTTTTGTGATCTAAAGAAAAATGTGGCCATGAAAATACTGGCATATCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGG  >  NC_000913/3803700‑3804178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑