Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,803,453	C→T	E229K (GAA→AAA)	waaB ←	UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,803,453	0	C	T	100.0%	105.2 / NA	32	E229K (GAA→AAA)	waaB	UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/18);  total (14/18)

ATATCTCCCGGTTGATACAGATGGCCATTGACATCAGGTTGAATAATGTCAGCAGGCCCGGAGACACAATCGGCACTGATACAAGGAATCCCCCACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTCTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGCGCGGGAATGACTGAGTCTTTAGTTTCGACGGGATTAAAAATAACATTTATCGTCGATTCTGCTAC  >  NC_000913/3803212‑3803687                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                           atatCTCCCGGTTGATACAGCAGGCCATTGACATCAGGTTCAATAATGTCAGCAGGCCCGGAGACACAATCGGCACTGATACAAGGAATCCCCCACGACAAGGCTTCAAGTAGGGCCATTGGGAAACCTTCATAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGATGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACACTTCGATCTTCAATATTTAATTTTCTACCAt                                                                                                                                                                                                                                    >  2:320162/1‑250 (MQ=255)   atCTCCCGGTTGATACAGATGGCCATTGACATCAGGTTGAATAATGTCAGCAGGCCCGGAGACACAATCGGCACTGCTACAAGGAATCCCCCACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATa                                                                                                                                                                                                                                   >  1:359595/1‑249 (MQ=255)       cccGGTTGATACAGATGGCCATTGACATCAGGTTGAATAATGTCAGCAGGCCCGGAGACACAATCGGCACTGATACAAGGAATCCCCCACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCTCTTTTTCAATATCTTGCTGGGCCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAg                                                                                                                                                                                                                                 <  1:88452/247‑1 (MQ=255)                                               tGTCAGCAGGCCCGGAGACACAATCGGCACTGATACAAGGAATCCCCCACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGt                                                                                                                                                                                     >  1:466557/1‑251 (MQ=255)                                                          ccGGAGACACAATCGGCACTGATACAAGGAATCCCCCACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTAcc                                                                                                                                                                          <  1:492758/251‑1 (MQ=255)                                                             gagaCACAATCGGCACTGATACAAGGAATCCCCCACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTg                                                                                                                                                                                                                             <  2:538990/197‑1 (MQ=255)                                                             gagaCACAATCGGCACTGATACAAGGAATCCCCCACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTg                                                                                                                                                                                                                             >  1:538990/1‑197 (MQ=255)                                                                       cGGCACTGATACAAGGAATCCCCCACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAAGATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGa                                                                                                                                                              <  1:50015/250‑1 (MQ=255)                                                                        ggCACTGATACAAGGAATCCCCCACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGAt                                                                                                                                                             >  2:163948/1‑250 (MQ=255)                                                                                 aCAAGGAATCCCCCACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCa                                                                                                                                                    <  1:403948/250‑1 (MQ=255)                                                                                      gAATCCCCCACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATGTAATTTTCTACCATAAGCCTGACATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGGTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAgg                                                                                                                                              <  2:359595/251‑1 (MQ=255)                                                                                          cccccACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCtt                                                                                                                                          >  1:217632/1‑251 (MQ=255)                                                                                          cccccACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATAGCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAAGCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCtt                                                                                                                                          <  2:387098/251‑1 (MQ=255)                                                                                                      ggCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCt                                                                                                                              >  2:187750/1‑251 (MQ=255)                                                                                                        cTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTgg                                                                                                                            >  2:19166/1‑251 (MQ=255)                                                                                                          tCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCGTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGc                                                                                                                           <  2:77038/250‑1 (MQ=255)                                                                                                                             aaCCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATc                                                                                                                                                                                                              <  1:162804/148‑1 (MQ=255)                                                                                                                                              gTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGaaa                                                                                                                                                                                                                >  2:139048/1‑129 (MQ=255)                                                                                                                                              gTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGaaa                                                                                                                                                                                                                <  1:139048/129‑1 (MQ=255)                                                                                                                                               tGAGCAATAAAGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAACTAAACGTTGcc                                                                                     >  1:475459/1‑251 (MQ=255)                                                                                                                                                gAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGcc                                                                                     <  1:368230/250‑1 (MQ=255)                                                                                                                                                           cactcactTTTTCAATACCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTcc                                                                         >  1:129025/1‑251 (MQ=255)                                                                                                                                                               cactTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTTATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCg                                                                      <  2:333034/250‑1 (MQ=255)                                                                                                                                                               cactTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCgg                                                                     <  1:187750/251‑1 (MQ=255)                                                                                                                                                               cactTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCg                                                                      <  1:221069/250‑1 (MQ=255)                                                                                                                                                                    tttCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGcgcg                                                                >  1:215454/1‑251 (MQ=255)                                                                                                                                                                           atCTTGCTGGACCAATTCCAAGGGATATTGTCGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGACTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGCGCGGGAATg                                                          >  2:348237/1‑250 (MQ=255)                                                                                                                                                                                     aCCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGCGCGGGAATGACTGAGTCttt                                               <  2:466557/251‑1 (MQ=255)                                                                                                                                                                                                              aaCCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCTCCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGACGTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTGCCTCGGGCGCGGGAATGACTGAGTCTTTAGTGTCGACGGGATTAAAAATAACa                      <  1:348237/251‑1 (MQ=255)                                                                                                                                                                                                               aCCATACCAGACAATTCGATCATCAACATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTGCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGCGCGGGAATGACTGAGTCGTTAGTTTCGACGGGATTAAAAATAACAt                     <  2:217632/251‑1 (MQ=255)                                                                                                                                                                                                                    aCCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGCGCGGGAATGACTGAGTCTTTAGTTTCGACGGGATTAAAAATAACATTTAt                 <  1:83279/250‑1 (MQ=255)                                                                                                                                                                                                                                  tcatcaATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGCGCGGGAATGACTGAGTCTTTAGTTTCGACGGGATTAAAAATAACATTTATCGTCGATTCTGCTac  >  1:367718/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                           ATATCTCCCGGTTGATACAGATGGCCATTGACATCAGGTTGAATAATGTCAGCAGGCCCGGAGACACAATCGGCACTGATACAAGGAATCCCCCACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTCTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGCGCGGGAATGACTGAGTCTTTAGTTTCGACGGGATTAAAAATAACATTTATCGTCGATTCTGCTAC  >  NC_000913/3803212‑3803687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑