Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,744,369	C→T	A14A (GCC→GCT)	yiaM →	2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,744,369	0	C	T	100.0%	125.4 / NA	39	A14A (GCC→GCT)	yiaM	2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/25);  total (14/25)

GTTGTATTATGCAAACAGCCTCTGAGATACGAAAAATTGTGGTTAAAGTCGCATTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGATGAATTGTCACGTTATTTATTTGTCTGGTTAACGTTTATTGGCGCGATTGTAGCTTTTATGGATAACGCCCACGTTCAGGTCACCTTTCTGGTGGAGAAACTCTCCCCCGCATGGCAGCGACGAGTCGCTCTGGTTACCCATTCCTTAATCTTATTTATTTG  >  NC_000913/3744140‑3744602                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          gTTGTATTATGCAAACAGCCTCTGAGATACGAAAAATTGTGGTTAAAGTCGCATTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTAttt                                                                                                                                                                                                                      <  2:238555/251‑1 (MQ=255)    gTATTATGCAAAAAGCCTCTGAGATACGAAAAATTGTGGTTAAAGTCGCATTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTtat                                                                                                                                                                                                                    >  1:124703/1‑250 (MQ=255)        tatGCAAACAGCCTCTGAGATACGAAAAATTGTGGTTAAAGGCGCATTAACGGCGCTGAATTAAGCGAATAATCGTCTACGCCAGAACGCCTTGGTAATGTTATTGCTCTAAGTATATTAATAATTCAGGTTGCTGCACATGCGGCTTGAATTAGGACAGTCATATCCTCGAGGAATAGCTATGAAAAAAATACGCGAGGCTATTCGGGTGATTAAGCTCGCTGTACTTTCCTTTATGGGAGTTATAAAc                                                                                                                                                                                                                <  2:302304/250‑1 (MQ=255)               aCAGCCTCTGAGATACGAAAAATTGTGGTTAAAGTCGCATTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTAtttt                                                                                                                                                                                                        <  1:46931/251‑1 (MQ=255)                   ccTCTGAGATACGAAAAATTGTGGTTAAAGTCGCATTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGa                                                                                                                                                                                                    <  2:460452/251‑1 (MQ=255)                      ttGAGATACGAAAAATGGTGGTTAAAGCCGCATTACCGGCGCGGAATTAATCGAATAATCGTTTACGCAAGAACGCTTGGGTAATTTTATTGC‑ATTACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGata                                                                                                                                                                                                  <  1:152110/248‑1 (MQ=255)                                         gggtAAAGTCGCATTAACTGCTCTTAATTCATCTAATAATCGTGTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAAAAATTCAGGTGGCTGCACATGCGACTTGAATTACGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTAGCTGTACTTTCCTGTATTGGATTTATAAACATTATTTTAAGATATGGTTTGCAGACAAGCAtg                                                                                                                                                                               <  2:309669/247‑2 (MQ=255)                                          gTTAAAGTCGCATTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGtt                                                                                                                                                                                             >  2:430736/1‑235 (MQ=255)                                          gTTAAAGTCGCATTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGtt                                                                                                                                                                                             <  1:430736/235‑1 (MQ=255)                                           ttAACGTCGCATTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAAAGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGGATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCAtttt                                                                                                                                                                             >  2:322598/1‑250 (MQ=255)                                                  cGCATTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTtg                                                                                                                                                                     <  1:38928/251‑1 (MQ=255)                                                    cATTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTtgat                                                                                                                                                                   <  1:194175/251‑1 (MQ=255)                                                     aTTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTa                                                                                                                                                                            <  1:499656/241‑1 (MQ=255)                                                     aTTAACGGAGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTACTAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTa                                                                                                                                                                            >  2:499656/1‑241 (MQ=255)                                                       tAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTtgatga                                                                                                                                                                 <  2:52733/250‑1 (MQ=255)                                                        aaCGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTATAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGATGAAt                                                                                                                                                               <  2:141189/251‑1 (MQ=255)                                                                         cGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGATGAATTGTCACGTTatttattt                                                                                                                                              >  1:175013/1‑251 (MQ=255)                                                                          gAATAATCGTCTACGACAGAACGCCGTGGTAATGTTATTGCTCTAGCTATATTAATAATTCATGTTGCTGCACATGCGACTTGAATTATGACAGACATATCCTCAAGGAATAGCGATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATAGGGTTTTCTGACAAGCAATTTATCTGTTGATGAATTGTCACGTTATTTATTTg                                                                                                                                             <  1:558918/251‑1 (MQ=255)                                                                           aataatCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGTAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGATGAATTGTCACGTTATTTATTTGt                                                                                                                                            <  2:455552/251‑1 (MQ=255)                                                                              aatCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGATGAATTGTCACGTTATTTATTTGTCt                                                                                                                                          <  1:5471/250‑1 (MQ=255)                                                                                       cGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGATGAATTGTCACGTTATTTATTTGTCTGGTTAACGtt                                                                                                                                <  2:271358/251‑1 (MQ=255)                                                                                            gAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCTCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGTCAAGCATTTTATCTGTTGATGAATTGTCACGTTATTTATTTGTCTGGTTATCGTTTATTg                                                                                                                           <  2:187469/251‑1 (MQ=255)                                                                                             aaCGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGATGAATTGTCACGTTATTTATTTGTCTGGTTAACGTTTATTgg                                                                                                                          <  1:107567/251‑1 (MQ=255)                                                                                             aaCGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGATGAATTGTCACGTTATTTATTTGTCTGGTTAACGTTTATTgg                                                                                                                          <  1:128471/251‑1 (MQ=255)                                                                                               cGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGATGAATTGTCACGTTATTTATTTGTCTGGTTAACGTTTATTGgcg                                                                                                                        <  1:99170/251‑1 (MQ=255)                                                                                                       tAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGATGAATTGTCACGTTATTTATTTGTCTGGTTAACGTTTATTGGCGCGATTGt                                                                                                                 >  2:582058/1‑250 (MQ=255)                                                                                                         aTGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTTCACATGCGACGTGAATTATGACATACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAATCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTAGTTTATTTATAAACATTATTTTAAGATATGTGTTTCATACAAGCATTTCATCTGTTGATGAATTGTCACGTTATTTATTGGTCTGGTTAACGTTGATTGTCGCGTTTGTAGc                                                                                                              <  2:48717/251‑1 (MQ=255)                                                                                                            ttATTGCTCTAACTATATTAATAATTAAGGTTGCTGCACATGCGACTTGAATTATAACAGACATCACCTATAGGGATAGCTATGAACAAACTCCTCGAAGCAATACTGGCGATTAATCTCGTTGTACTTTCTTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGATGAATTATCACGTTATTTATTTGTATTGTTACAGTTTATTGGCGCGTTTGTAGCttt                                                                                                           >  2:546304/1‑251 (MQ=255)                                                                                                                tGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGATGAATTGTCACGTTATTTATTTGTCTGGTTAACGTTTATTGGCGCGATTGTAGCTTTTAt                                                                                                        <  1:318889/250‑1 (MQ=255)                                                                                                                  ctctAAATATATTAATAATTCACGTTGCTGCACATGCGACTTGAATTATGACAGCCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGATGAATTGTCACGTTATTTATTTGTCTGGTTAACGTTTATTGGCGCGATTGTAGCTTTTATgg                                                                                                      >  2:387376/1‑250 (MQ=255)                                                                                                                                                   aTGCGACTTGAATTATGACAGCCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGATGAATTGTCACGTTATTTATTTGTCTGGTTAACGTTTATTTGCGCGATTGTAGCTTTTATGGATAACGCCCACGTTCAGGTCACCTTTCtggtgg                                                                     >  1:286666/1‑250 (MQ=255)                                                                                                                                                        aCTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGATGAATTGTCACGTTATTTATTTGTCTGGTTAACGTTTATTGGCGCGATTGTAGCTTTTATGGATAACGCCCACGTTCAGGTCACCTTTCTGGTGGAGAAAc                                                               >  2:480861/1‑251 (MQ=255)                                                                                                                                                         cTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGATGAATTGTCACGTTATTTATTTGTCTGGTTAACGTTTATTGGCGCGATTGTAGCTTTTATGGATAACGCCCACGTTCAGGTCACCTTTCTGGTGGAGAAAct                                                              >  2:5563/1‑251 (MQ=255)                                                                                                                                                             aaTTATGACAGACATAGCCTCATGGAATAGCTATGAAAAAAATACTCGAAGCCATACTGGCGATTAATATCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATATGTTGATGAATTGTCACGTTATTTATTTGTCTGGTTAACGTTTATTGGCGCGATTGTAGCTTTTATGGATAACGCCCACGTTCAGGTCCCCTTTCTGGTGGAGAAACTCTcc                                                          >  1:497912/1‑251 (MQ=255)                                                                                                                                                                                      aaTAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGATGAATTGTCACGTTATTTATTTGTCTGGTTAACGTTTATTGGCGCGATTGTAGCTTTTATGGATAACGCCCACGTTCAGGTCACCTTTCTGGTGGAGAAACTCTCCCCCGCATGGCAGCGACGAGTCGctc                                 >  1:493720/1‑251 (MQ=255)                                                                                                                                                                                       aTAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCTGTTCTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGATGAATTGTCACGTTATTTATTTGTCTGGTTAACGTTTATTGGCGCGATTGTAGCTTTTATGGATAACGCCCACGTTCAGGTCACCTTTCTGGTGGAGAAACTCTCCCCCGCATGGCAGCGACGAGTCGctct                                <  1:571112/251‑1 (MQ=255)                                                                                                                                                                                                    aaaTACTCGAAGCAATACTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGATGAATTGTCACGTTATTTATTTGTCTGGTTAACGTTTATTGGCGCGATTGTAGCTTTTATGGATAACGCCCACGTTCAGGTCACCTTTCTGGTGGAGAAACTCTCCCCCGCATGGCAGCGACGAGTCGCTCTGGTTACCCATTcc                   <  1:322598/251‑1 (MQ=255)                                                                                                                                                                                                                    aCTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGATGAATTGTCACGTTATTTATTTGTCTGGTTAACGTTTATTGGCGCGATTGTAGCTTTTATGGATAACGCCCACGTTCAGGTCACCTTTCTGGTGGAGAAACTCTCCCCCGCATGGCAGCGACGAGTCGCTCTGGTTACCCATTCCTTAATCTtatttatt    <  1:138990/250‑1 (MQ=255)                                                                                                                                                                                                                     cTGGCGATTAATCTCGCTGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGATGAATTGTCACGTTATTTATTTGTCTGGTTAACGTTTATTGGCGCGATTGTAGCTTTTATGGATAACGCCCACGTTCAGGTCACCTTTCTGGTGGAGAAACTCTCCCCCGCATGGCAGCGACGAGTCGCTCTGGTTACCCATTCCTTAATCTTATTTATTtg  >  1:359105/1‑251 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          GTTGTATTATGCAAACAGCCTCTGAGATACGAAAAATTGTGGTTAAAGTCGCATTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGATGAATTGTCACGTTATTTATTTGTCTGGTTAACGTTTATTGGCGCGATTGTAGCTTTTATGGATAACGCCCACGTTCAGGTCACCTTTCTGGTGGAGAAACTCTCCCCCGCATGGCAGCGACGAGTCGCTCTGGTTACCCATTCCTTAATCTTATTTATTTG  >  NC_000913/3744140‑3744602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑