Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,713,979	C→T	P110S (CCG→TCG)	yiaC →	peptidyl‑lysine N‑acetyltransferase YiaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,713,979	0	C	T	97.7%	138.5 / ‑3.8	44	P110S (CCG→TCG)	yiaC	peptidyl‑lysine N‑acetyltransferase YiaC
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (23/20);  total (24/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.16e-01

AAAGCGAATTACTGGCGTGACTGCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAACCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCA  >  NC_000913/3713736‑3714223                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     aaaGCGAATTACTAGCGTGACTGCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGGCTGGGAAGAAGACGGTAAGCTTCTCGGATTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGACCGTCAGGCTCGGTATTGGTAAGTCGCTGCTGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGAt                                                                                                                                                                                                                                               >  1:432889/1‑251 (MQ=255)      gAATTACTGGCGTGACTGCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAAtt                                                                                                                                                                                                                                          >  1:230313/1‑251 (MQ=255)         ttACTGGCGTGACTGCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAAttttt                                                                                                                                                                                                                                       >  1:531830/1‑251 (MQ=255)              ggCGTGACTGCATTCCGCTGGTGCGGGATGCCTTTCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTAcccg                                                                                                                                                                                                                                  <  2:375523/251‑3 (MQ=255)                       gCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAggg                                                                                                                                                                                                                          <  2:155758/250‑1 (MQ=255)                                 ggTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCCCCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACAt                                                                                                                                                                                                                 <  2:13934/249‑1 (MQ=255)                                 ggTGCGGGATGCATATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGTTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTTGTAAGGCGCTGATGCAGTATGTGCAGCAGCTCCATCCCCACCTGATGCTGGAGGTTGAGCAAAAAAATCAATCGGCGATAAATTTTTACCATGCACAGGGTTTTCACATTg                                                                                                                                                                                                               <  2:480848/251‑1 (MQ=255)                                      gggATTCCTATCTTGCCAACGCGCAAAACTGGTTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTGCTGGCAGCGATGTTTGTCGCACCGAAGGCCTTCAGGCGCGGGATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTAACAAAAAAATCAATCGGCGATAAAATTTTACCAGGCACAGGGTTTGCACAGTGTCGAt                                                                                                                                                                                                          <  2:288684/251‑1 (MQ=255)                                         aTGCCTATCTTGCCAACGCGCACAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTAGCACCGAAGGCCGTCCGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACACGGTTTTCCCATTGTCGATTgc                                                                                                                                                                                                       >  2:519022/1‑251 (MQ=255)                                                 cTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGc                                                                                                                                                                                                >  1:51990/1‑250 (MQ=255)                                                          gcgcAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAAc                                                                                                                                                                                      <  1:205065/251‑1 (MQ=255)                                                            gcAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGCTGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGATGGAGGTTTATCAAAAAACTCAATCGGCGATAAATTTTTACCAGGCCCAGGGTTTTCACATTGTCGAATGCGCATGGCAGGCTGAAAccc                                                                                                                                                                                    >  2:490152/1‑251 (MQ=255)                                                            gcAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAAccc                                                                                                                                                                                    >  2:401163/1‑251 (MQ=255)                                                               aaaCTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGACGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAAc                                                                                                                                                                                 >  2:48996/1‑251 (MQ=255)                                                                    gggTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGGCGATTGCGCATGGCAGGATGAAACCCAACTAccc                                                                                                                                                                            <  2:535220/251‑1 (MQ=255)                                                                        cTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAACCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACAt                                                                                                                                                                        >  1:76027/1‑251 (MQ=255)                                                                                            ttgtcGGATTTGTCAGAATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCAGAGAAGGCCGTCCGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCACCACCTGATGATGGAGATTTATCAAAAAACTCAATCGGCGATACCTTTTTACCAGGCACAGGGTTTTCACATTGTCGATTTCGCATTGCAGGATGAAACCCACCTACCCAAATGGAATATGAGCTGGACggtg                                                                                                                                                    >  2:136601/4‑251 (MQ=255)                                                                                                      tGTCAGCATTATGGAAAGACGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTCTGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGAGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGc                                                                                                                                          >  2:274266/1‑251 (MQ=255)                                                                                                                  ggAAGGCCGATTTCGGGCAGCGATGTTTGGCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTCTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCGGTAAgtggca                                                                                                                               <  1:464856/250‑4 (MQ=255)                                                                                                                                    aGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTAttt                                                                                                            <  1:436423/251‑1 (MQ=255)                                                                                                                                          gTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTcc                                                                                                        >  2:268883/1‑249 (MQ=255)                                                                                                                                                   aCCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGGGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGcc                                                                                               >  2:444234/1‑249 (MQ=255)                                                                                                                                                       aaGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGcc                                                                                         >  1:434286/1‑251 (MQ=255)                                                                                                                                                        aGGCCTTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCg                                                                                        <  2:177368/251‑1 (MQ=255)                                                                                                                                                             gTCAGGCGCGGTATTGGTAAGGCGCTGATGAAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGtctt                                                                                    >  1:226255/1‑247 (MQ=255)                                                                                                                                                               cAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTAc                                                                                  <  2:473623/250‑1 (MQ=255)                                                                                                                                                               cAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTAc                                                                                  <  1:166048/250‑1 (MQ=255)                                                                                                                                                                           tGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGcc                                                                                         >  2:493849/1‑231 (MQ=255)                                                                                                                                                                           tGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGcc                                                                                         <  1:493849/231‑1 (MQ=255)                                                                                                                                                                                      tGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGACGATAAATTTTTACCAGGCACAGGGTTTTCACCTTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATAAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGAATTACCCGCACAGCCATTCCCCAGCcgcg                                                          >  1:279184/1‑251 (MQ=255)                                                                                                                                                                                       gATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGAcc                                                                                                                     <  1:101672/191‑1 (MQ=255)                                                                                                                                                                                       gATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGAcc                                                                                                                     >  2:101672/1‑191 (MQ=255)                                                                                                                                                                                                      gccgcgcCAGCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCACTTACCCTCATTGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCttt                                          <  1:482243/248‑1 (MQ=255)                                                                                                                                                                                                                ccccACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCCCAGGGTTTGCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGGTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACg                                <  2:242927/251‑1 (MQ=255)                                                                                                                                                                                                                ccccACCTGATGCTGGAGGTTTATAAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACg                                >  2:421944/1‑251 (MQ=255)                                                                                                                                                                                                                ccccACCGGATGCTTGAGGTTTATCAAAAAAATCAATCGGCGATAAATTGTTACCAGGCCCAGGTTTTTCACATTGGCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGTTTCAAACGCGGTAAGTTTCAGTTCCGGACTGTTGTATTTTTCCAGCCATGCCTGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCGGGGGAGATCTTTCGTCAGCACg                                <  2:395364/251‑1 (MQ=255)                                                                                                                                                                                                                       tGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTg                         >  1:275235/1‑251 (MQ=255)                                                                                                                                                                                                                        gATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTcc                                                                   >  1:278311/1‑208 (MQ=255)                                                                                                                                                                                                                        gATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTcc                                                                   <  2:278311/208‑1 (MQ=255)                                                                                                                                                                                                                                  gTTTATCAACAAAGTCAATCGGCGATAACTTTTTACCAGGCACAGGGTTTTAACATAGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGAGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTCCCCGCACAGCCATTCCCCCGCCGCGAGCTGGGGCGATCTTTGGTCAGCACGTTCACTGCGCCGgtttt               >  2:47667/1‑250 (MQ=255)                                                                                                                                                                                                                                  gTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTa              <  1:268883/251‑1 (MQ=255)                                                                                                                                                                                                                                  gTTTAGCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTT‑GGTCAGCACGTTCACTGCGCCGTTTTTAc             >  2:562743/1‑251 (MQ=255)                                                                                                                                                                                                                                        cAAAAAATTCAATCGGCGATAAATTTTTACCAGGCACAGGGTGTTCACATTGTCGATTGCGCATGGCAGGCTGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGCCCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTGTTTACAAAt         <  1:490152/250‑1 (MQ=255)                                                                                                                                                                                                                                           aaaaaTCAATCGGCGATAAATTTTTACCAGGCACAGGGTCTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCg     >  1:465375/1‑251 (MQ=255)                                                                                                                                                                                                                                              aatcaatcGGCGATAAATTATTACCAGGCACAGGGTGTTCACATTGTCGTTTGCGCATGGCAGGATGAAACCCAACTACCCACATGGACTATGAGCTGGCCGGTGGTGCAAACGGTGTAAGTTTCAGTTCCGGACCGTTGGATTTTTCCAGCCATGCCAGCGCCGTAGTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACCGCGCCGTTTTTACAAATACCGTCa  <  2:226255/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     AAAGCGAATTACTGGCGTGACTGCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAACCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCA  >  NC_000913/3713736‑3714223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑